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Protein

Glutamate receptor 3

Gene

Gria3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate (By similarity).By similarity

Miscellaneous

The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei474Glutamate1 Publication1
Binding sitei509Glutamate1 Publication1
Binding sitei731Glutamate1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-399710 Activation of AMPA receptors
R-MMU-399719 Trafficking of AMPA receptors
R-MMU-416993 Trafficking of GluR2-containing AMPA receptors
R-MMU-438066 Unblocking of NMDA receptor, glutamate binding and activation
R-MMU-8849932 Synaptic adhesion-like molecules

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor 3
Short name:
GluR-3
Alternative name(s):
AMPA-selective glutamate receptor 3
GluR-C
GluR-K3
Glutamate receptor ionotropic, AMPA 3
Short name:
GluA3
Gene namesi
Name:Gria3
Synonyms:Glur3, Kiaa4184
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:95810 Gria3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 546ExtracellularBy similarityAdd BLAST524
Transmembranei547 – 567HelicalBy similarityAdd BLAST21
Topological domaini568 – 596CytoplasmicBy similarityAdd BLAST29
Intramembranei597 – 612Helical; Pore-formingBy similarityAdd BLAST16
Intramembranei613 – 615By similarity3
Topological domaini616 – 621CytoplasmicBy similarity6
Transmembranei622 – 642HelicalBy similarityAdd BLAST21
Topological domaini643 – 817ExtracellularBy similarityAdd BLAST175
Transmembranei818 – 838Helical; Name=M4By similarityAdd BLAST21
Topological domaini839 – 888CytoplasmicBy similarityAdd BLAST50

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2096617

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000004223023 – 888Glutamate receptor 3Add BLAST866

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi57N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi85 ↔ 334By similarity
Glycosylationi260N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi374N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi409N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi416N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi615S-palmitoyl cysteineBy similarity1
Disulfide bondi744 ↔ 7991 Publication
Lipidationi841S-palmitoyl cysteine1 Publication1
Modified residuei871PhosphotyrosineCombined sources1
Modified residuei881PhosphotyrosineCombined sources1

Post-translational modificationi

Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-615 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-841 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9Z2W9
PeptideAtlasiQ9Z2W9
PRIDEiQ9Z2W9

PTM databases

iPTMnetiQ9Z2W9
PhosphoSitePlusiQ9Z2W9
SwissPalmiQ9Z2W9

Expressioni

Gene expression databases

BgeeiENSMUSG00000001986 Expressed in 212 organ(s), highest expression level in prefrontal cortex
CleanExiMM_GRIA3
ExpressionAtlasiQ9Z2W9 baseline and differential
GenevisibleiQ9Z2W9 MM

Interactioni

Subunit structurei

Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with PRKCABP, GRIP1 and GRIP2 (By similarity). Found in a complex with GRIA1, GRIA2, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with CACNG5 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207340, 7 interactors
IntActiQ9Z2W9, 7 interactors
MINTiQ9Z2W9
STRINGi10090.ENSMUSP00000075687

Structurei

Secondary structure

1888
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Z2W9
SMRiQ9Z2W9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Z2W9

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni502 – 504Glutamate binding3
Regioni680 – 681Glutamate binding2

Domaini

The M4 transmembrane segment mediates tetramerization and is required for cell surface expression.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1054 Eukaryota
ENOG410XPSH LUCA
GeneTreeiENSGT00910000143978
HOGENOMiHOG000234372
HOVERGENiHBG051839
InParanoidiQ9Z2W9
KOiK05199
TreeFamiTF315232

Family and domain databases

InterProiView protein in InterPro
IPR001828 ANF_lig-bd_rcpt
IPR019594 Glu/Gly-bd
IPR001508 Iono_rcpt_met
IPR001320 Iontro_rcpt
IPR028082 Peripla_BP_I
PfamiView protein in Pfam
PF01094 ANF_receptor, 1 hit
PF00060 Lig_chan, 1 hit
PF10613 Lig_chan-Glu_bd, 1 hit
PRINTSiPR00177 NMDARECEPTOR
SMARTiView protein in SMART
SM00918 Lig_chan-Glu_bd, 1 hit
SM00079 PBPe, 1 hit
SUPFAMiSSF53822 SSF53822, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.iShow all

Isoform Flip (identifier: Q9Z2W9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGQSVLRAVF FLVLGLLGHS HGGFPNTISI GGLFMRNTVQ EHSAFRFAVQ
60 70 80 90 100
LYNTNQNTTE KPFHLNYHVD HLDSSNSFSV TNAFCSQFSR GVYAIFGFYD
110 120 130 140 150
QMSMNTLTSF CGALHTSFVT PSFPTDADVQ FVIQMRPALK GAILSLLGYY
160 170 180 190 200
KWEKFVYLYD TERGFSILQA IMEAAVQNNW QVTARSVGNI KDIQEFRRII
210 220 230 240 250
EEMDRRQEKR YLIDCEVERI NTILEQVVIL GKHSRGYHYM LANLGFTDIV
260 270 280 290 300
LERVMHGGAN ITGFQIVNNE NPMVQQFIQR WVRLDEREFP EAKNAPLKYT
310 320 330 340 350
SALTHDAILV IAEAFRYLRR QRVDVSRRGS AGDCLANPAV PWSQGIDIER
360 370 380 390 400
ALKMVQVQGM TGNIQFDTYG RRTNYTIDVY EMKVSGSRKA GYWNEYERFV
410 420 430 440 450
PFSDQQISND SSSSENRTIV VTTILESPYV MYKKNHEQLE GNERYEGYCV
460 470 480 490 500
DLAYEIAKHV RIKYKLSIVG DGKYGARDPE TKIWNGMVGE LVYGRADIAV
510 520 530 540 550
APLTITLVRE EVIDFSKPFM SLGISIMIKK PQKSKPGVFS FLDPLAYEIW
560 570 580 590 600
MCIVFAYIGV SVVLFLVSRF SPYEWHLEDN NEEPRDPQSP PDPPNEFGIF
610 620 630 640 650
NSLWFSLGAF MQQGCDISPR SLSGRIVGGV WWFFTLIIIS SYTANLAAFL
660 670 680 690 700
TVERMVSPIE SAEDLAKQTE IAYGTLDSGS TKEFFRRSKI AVYEKMWSYM
710 720 730 740 750
KSAEPSVFTK TTADGVARVR KSKGKFAFLL ESTMNEYIEQ RKPCDTMKVG
760 770 780 790 800
GNLDSKGYGV ATPKGSALRT PVNLAVLKLS EQGILDKLKN KWWYDKGECG
810 820 830 840 850
AKDSGSKDKT SALSLSNVAG VFYILVGGLG LAMMVALIEF CYKSRAESKR
860 870 880
MKLTKNTQNF KPAPATNTQN YATYREGYNV YGTESVKI
Length:888
Mass (Da):100,527
Last modified:July 27, 2011 - v2
Checksum:i9F68C5D80E81DC02
GO
Isoform Flop (identifier: Q9Z2W9-2)
Sequence is not available
Length:
Mass (Da):

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B0QZW1B0QZW1_MOUSE
Glutamate receptor 3
Gria3
888Annotation score:
F2Z488F2Z488_MOUSE
Glutamate receptor 3
Gria3
818Annotation score:
B0QZW2B0QZW2_MOUSE
Glutamate receptor 3
Gria3
83Annotation score:

Sequence cautioni

The sequence BAD90527 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti300T → I in BAD90527 (Ref. 4) Curated1
Sequence conflicti769R → G in BAA37124 (Ref. 1) Curated1
Sequence conflicti769R → G in BAE06153 (Ref. 1) Curated1
Sequence conflicti769R → G in AAL90768 (PubMed:11471062).Curated1
Sequence conflicti769R → G in AAL90769 (PubMed:11471062).Curated1
Sequence conflicti769R → G in BAD90527 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022342 mRNA Translation: BAA37124.1
AB079072 mRNA Translation: BAE06153.1
AF483494 mRNA Translation: AAL90768.1
AF483495 mRNA Translation: AAL90769.1
AK220530 mRNA Translation: BAD90527.1 Different initiation.
CH466570 Genomic DNA Translation: EDL29035.1
AL672290, AL672232 Genomic DNA Translation: CAQ11290.1
AL672232, AL672290 Genomic DNA Translation: CAQ12028.1
BC129855 mRNA Translation: AAI29856.1
CCDSiCCDS30097.1 [Q9Z2W9-1]
RefSeqiNP_001268858.1, NM_001281929.1
NP_058582.3, NM_016886.4
XP_006541583.1, XM_006541520.3
XP_017174034.1, XM_017318545.1 [Q9Z2W9-1]
UniGeneiMm.327681

Genome annotation databases

EnsembliENSMUST00000076349; ENSMUSP00000075687; ENSMUSG00000001986 [Q9Z2W9-1]
GeneIDi53623
KEGGimmu:53623
UCSCiuc009tal.3 mouse [Q9Z2W9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGRIA3_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2W9
Secondary accession number(s): A2VDF4, Q5DTJ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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