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Protein

Protein transport protein Sec31A

Gene

Sec31a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.1 Publication

GO - Molecular functioni

  • structural molecule activity Source: GO_Central

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-204005 COPII-mediated vesicle transport
R-RNO-2132295 MHC class II antigen presentation
R-RNO-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein Sec31A
Alternative name(s):
SEC31-like protein 1
SEC31-related protein A
Vesicle-associated protein 1
Gene namesi
Name:Sec31a
Synonyms:Sec31l1, Vap1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi620233 Sec31a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002951501 – 1249Protein transport protein Sec31AAdd BLAST1249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei423Asymmetric dimethylarginineBy similarity1
Modified residuei526PhosphoserineCombined sources1
Modified residuei531PhosphoserineCombined sources1
Cross-linki647Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei799PhosphoserineBy similarity1
Modified residuei1190PhosphothreonineBy similarity1
Modified residuei1192PhosphoserineCombined sources1
Cross-linki1246Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex, leading to regulate the size of COPII coats.By similarity

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9Z2Q1
PRIDEiQ9Z2Q1

PTM databases

iPTMnetiQ9Z2Q1
PhosphoSitePlusiQ9Z2Q1

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSRNOG00000002251 Expressed in 10 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasiQ9Z2Q1 baseline and differential
GenevisibleiQ9Z2Q1 RN

Interactioni

Subunit structurei

COPII is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1. SEC13 and SEC31 make a 2:2 tetramer that forms the edge element of the COPII outer coat. The tetramer self-assembles in multiple copies to form the complete polyhedral cage. Interacts (via WD 8) with SEC13 (PubMed:10574704, PubMed:10788476). Interacts with PDCD6; interaction takes place in response to cytosolic calcium increase and leads to bridge together the BCR(KLHL12) complex and SEC31A, leading to monoubiquitination. Interacts with KLHL12 (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi250159, 2 interactors
IntActiQ9Z2Q1, 2 interactors
STRINGi10116.ENSRNOP00000003072

Structurei

3D structure databases

ProteinModelPortaliQ9Z2Q1
SMRiQ9Z2Q1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati4 – 47WD 1Add BLAST44
Repeati64 – 111WD 2Add BLAST48
Repeati120 – 160WD 3Add BLAST41
Repeati166 – 206WD 4Add BLAST41
Repeati209 – 254WD 5Add BLAST46
Repeati258 – 298WD 6Add BLAST41
Repeati301 – 342WD 7Add BLAST42
Repeati397 – 429WD 8; interaction with SEC13PROSITE-ProRule annotationAdd BLAST33

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni161 – 470Interaction with SEC13By similarityAdd BLAST310
Regioni800 – 1142Interaction with PDCD6By similarityAdd BLAST343

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi873 – 879ALG-2-binding site motif-2 (ABS-2),By similarity7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi808 – 1120Pro-richAdd BLAST313

Domaini

The ALG-2-binding site motif-2 (ABS-2) contains a PXPGF sequence that binds hydrophobic pocket 3 of PDCD6.By similarity

Sequence similaritiesi

Belongs to the WD repeat SEC31 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0307 Eukaryota
ENOG410XQ1D LUCA
GeneTreeiENSGT00390000003175
HOGENOMiHOG000230582
HOVERGENiHBG055626
InParanoidiQ9Z2Q1
KOiK14005
PhylomeDBiQ9Z2Q1

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR024298 ACE1_Sec16_Sec31
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF12931 Sec16_C, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Z2Q1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKLKEIDRTA MQAWSPAQNH PIYLATGTSA QQLDATFSTN ASLEIFELDL
60 70 80 90 100
SDPSLDMKSC ATFSSSHRYH KLIWGPHKMD SKGDVSGVLI AGGENGNIIL
110 120 130 140 150
YDPSKIIAGD KEVVIAQKDK HTGPVRALDV NIFQTNLVAS GANESEIYIW
160 170 180 190 200
DLNNFATPMT PGAKTQPPED ISCIAWNRQV QHILASASPS GRATVWDLRK
210 220 230 240 250
NEPIIKVSDH SNRMHCSGLA WHPDVATQMV LASEDDRLPV VQMWDLRFAS
260 270 280 290 300
SPLRVLENHA RGILAIAWSM ADPELLLSCG KDAKILCSNP NTGEVLYELP
310 320 330 340 350
TNTQWCFDIQ WCPRNPAVLS AASFDGRIRV YSIMGGSIDG LRQKQVDKLS
360 370 380 390 400
SSFGNLDPFG TGQPLPPLQI PQQTSQHSIV LPLKKPPKWI RRPVGASFSF
410 420 430 440 450
GGKLVTFENV TGQPQQGAEQ PRRQPVFISQ VVTEKDFLSR SEQLQHVVQS
460 470 480 490 500
QGFISYCQKK IDASQTDFEK NVWSFLKVNF EEDSRGKYLE LLGYRREDLG
510 520 530 540 550
EKIALALNRV DGSDVALKDS DRVAQSDGEE SPAEEGQLLG ERIKEEKQEC
560 570 580 590 600
DFLPSAGGGT FNISVSGDID GLITRALLTG NFESAVDLCL HDNRMADAII
610 620 630 640 650
LAIAGGQELL AQTQKKYFAK SQSKITRLIT AVVMKNWKEI VESCDLKNWR
660 670 680 690 700
EALAAVLTYA KPDEFSALCD LLGARLESEG DSLLRTQACL CYICAGNVER
710 720 730 740 750
LVACWTKAQD GSNPLSLQDL IEKVVILRKA VQLTQALDTN TVGALLAEKM
760 770 780 790 800
SQYANLLAAQ GSIAAALAFL PDNTNQPDIV QLRDRLCRAQ GRSVPGQESS
810 820 830 840 850
RSSYEGQPLP KGGPGPLAGH PQVSRVQSQQ YYPQVRIAPT VTTWSDRTPT
860 870 880 890 900
ALPSHPPAAC PSDTQGGNPP PPGFIMHGNV VPNSPAPLPT SPGHMHSQPP
910 920 930 940 950
PYPQPQPYQP AQQYSLGTGG SAVYRPQQPV APPASKRYPN APYVSPVASY
960 970 980 990 1000
SGQPHMYTAQ PASSPTSSSA PLPPPPSSGA SFQHGGPGAP PSSSAYALPP
1010 1020 1030 1040 1050
GTTGTPPAAS ELPASQRTGP QNGWNDPPAL NRVPKKKKLP ENFMPPVPIT
1060 1070 1080 1090 1100
SPIMNPGGDP QPQGLQQQPS ASGPRSSHAS FPQPHLAGGQ PFHGIQQPLA
1110 1120 1130 1140 1150
QTGMPPSFSK PNTEGAPGAP IGNTIQHVQA LPTEKITKKP IPDEHLILKT
1160 1170 1180 1190 1200
TFEDLIQRCL SSATDPQTKR KLDDASKRLE CLYDKLRDQT LSPTIISGLH
1210 1220 1230 1240
SIARSIETRN YSEGLTVHTH IVSTSNFSET SAFMPVLKVV LSQASKLGV
Length:1,249
Mass (Da):135,272
Last modified:July 10, 2007 - v2
Checksum:i8069E577903231B5
GO
Isoform 2 (identifier: Q9Z2Q1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     503-541: Missing.
     834-864: Missing.
     907-1019: PYQPAQQYSL...SELPASQRTG → R

Note: No experimental confirmation available.
Show »
Length:1,067
Mass (Da):116,879
Checksum:iFCC63F7487497926
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V699G3V699_RAT
Protein transport protein Sec31A
Sec31a Sec31l1, rCG_37919
1,249Annotation score:
A0A0G2K0X9A0A0G2K0X9_RAT
Protein transport protein Sec31A
Sec31a
1,216Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti209D → N in AAD01990 (PubMed:10574704).Curated1
Sequence conflicti213R → W in AAD01990 (PubMed:10574704).Curated1
Sequence conflicti449Q → E in AAD01990 (PubMed:10574704).Curated1
Sequence conflicti479N → K in AAD01990 (PubMed:10574704).Curated1
Sequence conflicti817L → F in AAD01990 (PubMed:10574704).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_026754503 – 541Missing in isoform 2. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_026755834 – 864Missing in isoform 2. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_026756907 – 1019PYQPA…SQRTG → R in isoform 2. 1 PublicationAdd BLAST113

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034582 mRNA Translation: AAD01990.1
BC085722 mRNA Translation: AAH85722.1
PIRiT14150
RefSeqiNP_148981.1, NM_033021.1
XP_006250751.1, XM_006250689.3 [Q9Z2Q1-2]
XP_006250752.1, XM_006250690.2 [Q9Z2Q1-2]
UniGeneiRn.6322

Genome annotation databases

EnsembliENSRNOT00000033489; ENSRNOP00000038196; ENSRNOG00000002251 [Q9Z2Q1-2]
GeneIDi93646
KEGGirno:93646

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034582 mRNA Translation: AAD01990.1
BC085722 mRNA Translation: AAH85722.1
PIRiT14150
RefSeqiNP_148981.1, NM_033021.1
XP_006250751.1, XM_006250689.3 [Q9Z2Q1-2]
XP_006250752.1, XM_006250690.2 [Q9Z2Q1-2]
UniGeneiRn.6322

3D structure databases

ProteinModelPortaliQ9Z2Q1
SMRiQ9Z2Q1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250159, 2 interactors
IntActiQ9Z2Q1, 2 interactors
STRINGi10116.ENSRNOP00000003072

PTM databases

iPTMnetiQ9Z2Q1
PhosphoSitePlusiQ9Z2Q1

Proteomic databases

PaxDbiQ9Z2Q1
PRIDEiQ9Z2Q1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000033489; ENSRNOP00000038196; ENSRNOG00000002251 [Q9Z2Q1-2]
GeneIDi93646
KEGGirno:93646

Organism-specific databases

CTDi22872
RGDi620233 Sec31a

Phylogenomic databases

eggNOGiKOG0307 Eukaryota
ENOG410XQ1D LUCA
GeneTreeiENSGT00390000003175
HOGENOMiHOG000230582
HOVERGENiHBG055626
InParanoidiQ9Z2Q1
KOiK14005
PhylomeDBiQ9Z2Q1

Enzyme and pathway databases

ReactomeiR-RNO-204005 COPII-mediated vesicle transport
R-RNO-2132295 MHC class II antigen presentation
R-RNO-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Miscellaneous databases

PROiPR:Q9Z2Q1

Gene expression databases

BgeeiENSRNOG00000002251 Expressed in 10 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasiQ9Z2Q1 baseline and differential
GenevisibleiQ9Z2Q1 RN

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR024298 ACE1_Sec16_Sec31
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF12931 Sec16_C, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSC31A_RAT
AccessioniPrimary (citable) accession number: Q9Z2Q1
Secondary accession number(s): Q5RKK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: November 7, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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