Vdac1

Functionⁱ

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:25628567). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (By similarity).By similarity1 Publication

Activity regulationⁱ

Inhibited by nitric oxide.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	73	Critical for channel structural stability and gatingBy similarity		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	242 – 244	NADBy similarity		3
Nucleotide bindingⁱ	260 – 264	NADBy similarity		5

GO - Molecular functionⁱ

anion channel activity
Source: RGD
Inferred from direct assayⁱ
- 12438411
calcium channel activity Source: Reactome
nucleotide binding Source: UniProtKB-KW
porin activity Source: UniProtKB-KW
protein-containing complex binding
Source: RGD
Inferred from direct assayⁱ
- 9459579
voltage-gated anion channel activity
Source: RGD
Inferred from direct assayⁱ
- 12438411
- 15351738

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

apoptotic process Source: UniProtKB
behavioral fear response Source: Ensembl
learning Source: Ensembl
mitochondrial calcium ion transmembrane transport
Source: RGD
Inferred from direct assayⁱ
- 12438411
negative regulation of reactive oxygen species metabolic process Source: Ensembl
neuron-neuron synaptic transmission Source: Ensembl
regulation of autophagy of mitochondrion Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Porin
Biological process	Apoptosis, Ion transport, Transport
Ligand	NAD, Nucleotide-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Voltage-dependent anion-selective channel protein 1 Short name: VDAC-1 Short name: rVDAC1 Alternative name(s): Outer mitochondrial membrane protein porin 1
Gene namesⁱ	Name:Vdac1
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 10

Organism-specific databases

Rat genome database More...RGDⁱ	621575 Vdac1

RGDⁱ

621575 Vdac1

Topology

Feature key	Position(s)	DescriptionActions	Length
Transmembraneⁱ	26 – 35	Beta strandedBy similarity	10
Transmembraneⁱ	39 – 47	Beta strandedBy similarity	9
Transmembraneⁱ	54 – 64	Beta strandedBy similarityAdd BLAST	11
Transmembraneⁱ	69 – 76	Beta strandedBy similarity	8
Transmembraneⁱ	80 – 89	Beta strandedBy similarity	10
Transmembraneⁱ	95 – 104	Beta strandedBy similarity	10
Transmembraneⁱ	111 – 120	Beta strandedBy similarity	10
Transmembraneⁱ	123 – 130	Beta strandedBy similarity	8
Transmembraneⁱ	137 – 145	Beta strandedBy similarity	9
Transmembraneⁱ	150 – 158	Beta strandedBy similarity	9
Transmembraneⁱ	163 – 175	Beta strandedBy similarityAdd BLAST	13
Transmembraneⁱ	178 – 185	Beta strandedBy similarity	8
Transmembraneⁱ	189 – 198	Beta strandedBy similarity	10
Transmembraneⁱ	202 – 211	Beta strandedBy similarity	10
Transmembraneⁱ	218 – 227	Beta strandedBy similarity	10
Transmembraneⁱ	231 – 238	Beta strandedBy similarity	8
Transmembraneⁱ	242 – 251	Beta strandedBy similarity	10
Transmembraneⁱ	254 – 263	Beta strandedBy similarity	10
Transmembraneⁱ	273 – 282	Beta strandedBy similarity	10

Keywords - Cellular componentⁱ

Cell membrane, Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	137	S → E: Channel conformation is shifted toward the open state. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000050503	2 – 283	Voltage-dependent anion-selective channel protein 1Add BLAST		282

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylalanine1 Publication	1
Modified residueⁱ	13	Phosphoserine1 Publication	1
Modified residueⁱ	19	PhosphothreonineCombined sources	1
Modified residueⁱ	20	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	20	N6-succinyllysine; alternateBy similarity	1
Cross-linkⁱ	20	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linkⁱ	53	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residueⁱ	67	PhosphotyrosineBy similarity	1
Modified residueⁱ	107	PhosphothreonineBy similarity	1
Modified residueⁱ	109	N6-acetyllysine; alternateBy similarity	1
Cross-linkⁱ	109	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linkⁱ	110	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residueⁱ	137	Phosphoserine1 Publication	1
Cross-linkⁱ	161	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residueⁱ	193	Phosphoserine; by NEK1By similarity	1
Modified residueⁱ	240	PhosphoserineBy similarity	1
Modified residueⁱ	252	N6-acetyllysineBy similarity	1
Modified residueⁱ	266	N6-acetyllysine; alternateBy similarity	1
Cross-linkⁱ	266	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity

Post-translational modificationⁱ

Phosphorylation at Ser-193 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.By similarity

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.By similarity

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q9Z2L0
PRIDEⁱ	Q9Z2L0

2D gel databases

The World-2DPAGE database More...World-2DPAGEⁱ	0004:Q9Z2L0

World-2DPAGEⁱ

0004:Q9Z2L0

PTM databases

CarbonylDBⁱ	Q9Z2L0
iPTMnetⁱ	Q9Z2L0
PhosphoSitePlusⁱ	Q9Z2L0
SwissPalmⁱ	Q9Z2L0

Expressionⁱ

Tissue specificityⁱ

Widely expressed. High levels in heart and kidney with lower levels in brain and ascitic tumor. Very low levels in liver.1 Publication

Gene expression databases

Bgeeⁱ	ENSRNOG00000006375 Expressed in 10 organ(s), highest expression level in skeletal muscle tissue
Genevisibleⁱ	Q9Z2L0 RN

Interactionⁱ

Subunit structureⁱ

Interacts with hexokinases including HK1. The HK1-VDAC1 complex interacts with ATF2. Interacts with BCL2L1. Interacts with BAK1. Interacts with RTL10/BOP (via BH3 domain). Interacts with amyloid-beta and APP; induces VDAC1 dephosphorylation. Component of the mitochondrial permeability transition pore complex (mPTPC), at least composed of SPG7, VDAC1 and PPIF. Interacts with SPG7, NIPSNAP2 and SLC25A30.By similarity

Protein-protein interaction databases

BioGridⁱ	249746, 4 interactors
CORUMⁱ	Q9Z2L0
Database of interacting proteins More...DIPⁱ	DIP-37068N
IntActⁱ	Q9Z2L0, 8 interactors
Molecular INTeraction database More...MINTⁱ	Q9Z2L0
STRINGⁱ	10116.ENSRNOP00000008477

Structureⁱ

3D structure databases

SMRⁱ	Q9Z2L0
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domainⁱ

Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity (By similarity).By similarity

Sequence similaritiesⁱ

Belongs to the eukaryotic mitochondrial porin family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGⁱ	KOG3126 Eukaryota ENOG410ZBK1 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000011336
HOVERGENⁱ	HBG054036
InParanoidⁱ	Q9Z2L0
KEGG Orthology (KO) More...KOⁱ	K05862
OMAⁱ	KYKWAEH
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0F0O
PhylomeDBⁱ	Q9Z2L0
TreeFamⁱ	TF315091

Family and domain databases

Gene3Dⁱ	2.40.160.10, 1 hit
InterProⁱ	View protein in InterPro IPR023614 Porin_dom_sf IPR001925 Porin_Euk IPR027246 Porin_Euk/Tom40 IPR030270 VDAC1
PANTHERⁱ	PTHR11743 PTHR11743, 1 hit PTHR11743:SF13 PTHR11743:SF13, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01459 Porin_3, 1 hit
PRINTSⁱ	PR00185 EUKARYTPORIN
PROSITEⁱ	View protein in PROSITE PS00558 EUKARYOTIC_PORIN, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9Z2L0-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAVPPTYADL GKSARDVFTK GYGFGLIKLD LKTKSENGLE FTSSGSANTE 
        60         70         80         90        100
TTKVNGSLET KYRWTEYGLT FTEKWNTDNT LGTEITVEDQ LARGLKLTFD 
       110        120        130        140        150
SSFSPNTGKK NAKIKTGYKR EHINLGCDVD FDIAGPSIRG ALVLGYEGWL 
       160        170        180        190        200
AGYQMNFETS KSRVTQSNFA VGYKTDEFQL HTNVNDGTEF GGSIYQKVNK 
       210        220        230        240        250
KLETAVNLAW TAGNSNTRFG IAAKYQVDPD ACFSAKVNNS SLIGLGYTQT 
       260        270        280 
LKPGIKLTLS ALLDGKNVNA GGHKLGLGLE FQA

Length:283

Mass (Da):30,756

Last modified:January 23, 2007 - v4

Checksum:ⁱD32B05B8A8D75732

GO

Sequence cautionⁱ

The sequence AAH60558 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	55	N → T in AAD02476 (PubMed:9714728).Curated	1
Sequence conflictⁱ	83	T → S in AAD02476 (PubMed:9714728).Curated	1
Sequence conflictⁱ	129	V → M in AAD02476 (PubMed:9714728).Curated	1
Sequence conflictⁱ	148	G → GFDIAGPSIRGALVLGYEG in AAD02476 (PubMed:9714728).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF048828 mRNA Translation: AAD02476.1 AB039662 mRNA Translation: BAB13473.1 AF268467 mRNA Translation: AAF80115.1 BC060558 mRNA Translation: AAH60558.1 Different initiation. BC072484 mRNA Translation: AAH72484.2 BC087573 mRNA Translation: AAH87573.2 BC087657 mRNA Translation: AAH87657.2 BC104684 mRNA Translation: AAI04685.2 BC127491 mRNA Translation: AAI27492.1
NCBI Reference Sequences More...RefSeqⁱ	NP_112643.1, NM_031353.1
UniGeneⁱ	Rn.54594

Genome annotation databases

Ensemblⁱ	ENSRNOT00000008477; ENSRNOP00000008477; ENSRNOG00000006375
GeneIDⁱ	83529
KEGGⁱ	rno:83529
UCSC genome browser More...UCSCⁱ	RGD:621575 rat

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9Z2L0	Isoform Mt-VDAC1 of Voltage-dependent anion-selective channel protein 1		MOUSE		283	UniRef100_Q9Z2L0
UPI0003D75DBC		MOUSE		365

Protein	Similar proteins		Species	Score	Length	Source
Q9Z2L0	Voltage-dependent anion-selective channel protein 1	)	HUMAN		283	UniRef90_P21796
Voltage-dependent anion-selective channel protein 1	)	MOUSE		296
Voltage-dependent anion-selective channel protein 1		PIG		283
VDAC1 isoform 1		PANTR		283
voltage-dependent anion-selective channel protein 1 isoform X1		LIPVE		283
+46

Protein	Similar proteins		Species	Score	Length	Source
Q9Z2L0	Voltage-dependent anion-selective channel protein 1	)	HUMAN		283	UniRef50_P21796
Voltage-dependent anion-selective channel protein 1	)	MOUSE		296
Voltage-dependent anion-selective channel protein 1		PIG		283
Voltage-dependent anion-selective channel protein 2		BOVIN		294
Voltage-dependent anion-selective channel protein 2		RABIT		294
+185

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF048828 mRNA Translation: AAD02476.1 AB039662 mRNA Translation: BAB13473.1 AF268467 mRNA Translation: AAF80115.1 BC060558 mRNA Translation: AAH60558.1 Different initiation. BC072484 mRNA Translation: AAH72484.2 BC087573 mRNA Translation: AAH87573.2 BC087657 mRNA Translation: AAH87657.2 BC104684 mRNA Translation: AAI04685.2 BC127491 mRNA Translation: AAI27492.1
RefSeqⁱ	NP_112643.1, NM_031353.1
UniGeneⁱ	Rn.54594

3D structure databases

SMRⁱ	Q9Z2L0
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	249746, 4 interactors
CORUMⁱ	Q9Z2L0
DIPⁱ	DIP-37068N
IntActⁱ	Q9Z2L0, 8 interactors
MINTⁱ	Q9Z2L0
STRINGⁱ	10116.ENSRNOP00000008477

PTM databases

CarbonylDBⁱ	Q9Z2L0
iPTMnetⁱ	Q9Z2L0
PhosphoSitePlusⁱ	Q9Z2L0
SwissPalmⁱ	Q9Z2L0

2D gel databases

World-2DPAGEⁱ	0004:Q9Z2L0

World-2DPAGEⁱ

0004:Q9Z2L0

Proteomic databases

PaxDbⁱ	Q9Z2L0
PRIDEⁱ	Q9Z2L0

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSRNOT00000008477; ENSRNOP00000008477; ENSRNOG00000006375
GeneIDⁱ	83529
KEGGⁱ	rno:83529
UCSCⁱ	RGD:621575 rat

Organism-specific databases

CTDⁱ	7416
RGDⁱ	621575 Vdac1

Phylogenomic databases

eggNOGⁱ	KOG3126 Eukaryota ENOG410ZBK1 LUCA
GeneTreeⁱ	ENSGT00390000011336
HOVERGENⁱ	HBG054036
InParanoidⁱ	Q9Z2L0
KOⁱ	K05862
OMAⁱ	KYKWAEH
OrthoDBⁱ	EOG091G0F0O
PhylomeDBⁱ	Q9Z2L0
TreeFamⁱ	TF315091

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9Z2L0

PROⁱ

PR:Q9Z2L0

Gene expression databases

Bgeeⁱ	ENSRNOG00000006375 Expressed in 10 organ(s), highest expression level in skeletal muscle tissue
Genevisibleⁱ	Q9Z2L0 RN

Family and domain databases

Gene3Dⁱ	2.40.160.10, 1 hit
InterProⁱ	View protein in InterPro IPR023614 Porin_dom_sf IPR001925 Porin_Euk IPR027246 Porin_Euk/Tom40 IPR030270 VDAC1
PANTHERⁱ	PTHR11743 PTHR11743, 1 hit PTHR11743:SF13 PTHR11743:SF13, 1 hit
Pfamⁱ	View protein in Pfam PF01459 Porin_3, 1 hit
PRINTSⁱ	PR00185 EUKARYTPORIN
PROSITEⁱ	View protein in PROSITE PS00558 EUKARYOTIC_PORIN, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	VDAC1_RAT
Accessionⁱ	Q9Z2L0Primary (citable) accession number: Q9Z2L0 Secondary accession number(s): A1L125 , Q3MHT8, Q5M944, Q5M972, Q6IN28, Q6P9W9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
	Last sequence update:	January 23, 2007
	Last modified:	November 7, 2018
	This is version 138 of the entry and version 4 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

UniProtKB

UniParc

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Supporting data

UniProtKB - Q9Z2L0 (VDAC1_RAT)

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Voltage-dependent anion-selective channel protein 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Mitochondrion

Other locations

Mitochondrion

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ