UniProtKB - Q9Z2L0 (VDAC1_RAT)
Voltage-dependent anion-selective channel protein 1
Vdac1
Functioni
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 73 | Involved in ceramide and phosphatidylcholine binding. Critical for channel structural stability and gatingBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 242 – 244 | NADBy similarity | 3 | |
Nucleotide bindingi | 260 – 264 | NADBy similarity | 5 |
GO - Molecular functioni
- anion channel activity Source: RGD
- calcium channel activity Source: Reactome
- ceramide binding Source: Ensembl
- cholesterol binding Source: Ensembl
- identical protein binding Source: Ensembl
- ion channel binding Source: RGD
- nucleotide binding Source: UniProtKB-KW
- phosphatidylcholine binding Source: Ensembl
- porin activity Source: UniProtKB-KW
- protein-containing complex binding Source: RGD
- protein kinase binding Source: RGD
- voltage-gated anion channel activity Source: RGD
GO - Biological processi
- anion transport Source: RGD
- apoptotic process Source: UniProtKB
- behavioral fear response Source: RGD
- chemical synaptic transmission Source: RGD
- epithelial cell differentiation Source: RGD
- learning Source: RGD
- mitochondrial calcium ion transmembrane transport Source: RGD
- negative regulation of reactive oxygen species metabolic process Source: RGD
- neuron-neuron synaptic transmission Source: RGD
- regulation of autophagy of mitochondrion Source: RGD
Keywordsi
Molecular function | Porin |
Biological process | Apoptosis, Ion transport, Transport |
Ligand | Lipid-binding, NAD, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-5205685, PINK1-PRKN Mediated Mitophagy R-RNO-5689880, Ub-specific processing proteases R-RNO-70268, Pyruvate metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Voltage-dependent anion-selective channel protein 1Short name: VDAC-1 Short name: rVDAC1 Alternative name(s): Outer mitochondrial membrane protein porin 1 |
Gene namesi | Name:Vdac1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621575, Vdac1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Mitochondrion
- Mitochondrion outer membrane By similarity; Multi-pass membrane protein By similarity
Other locations
- Membrane raft By similarity; Multi-pass membrane protein By similarity
Mitochondrion
- mitochondrial nucleoid Source: RGD
- mitochondrial outer membrane Source: RGD
- mitochondrial permeability transition pore complex Source: UniProtKB
- mitochondrion Source: RGD
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- membrane Source: UniProtKB
- membrane raft Source: UniProtKB-SubCell
- protein-containing complex Source: RGD
- synaptic vesicle Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 26 – 35 | Beta strandedBy similarity | 10 | |
Transmembranei | 39 – 47 | Beta strandedBy similarity | 9 | |
Transmembranei | 54 – 64 | Beta strandedBy similarityAdd BLAST | 11 | |
Transmembranei | 69 – 76 | Beta strandedBy similarity | 8 | |
Transmembranei | 80 – 89 | Beta strandedBy similarity | 10 | |
Transmembranei | 95 – 104 | Beta strandedBy similarity | 10 | |
Transmembranei | 111 – 120 | Beta strandedBy similarity | 10 | |
Transmembranei | 123 – 130 | Beta strandedBy similarity | 8 | |
Transmembranei | 137 – 145 | Beta strandedBy similarity | 9 | |
Transmembranei | 150 – 158 | Beta strandedBy similarity | 9 | |
Transmembranei | 163 – 175 | Beta strandedBy similarityAdd BLAST | 13 | |
Transmembranei | 178 – 185 | Beta strandedBy similarity | 8 | |
Transmembranei | 189 – 198 | Beta strandedBy similarity | 10 | |
Transmembranei | 202 – 211 | Beta strandedBy similarity | 10 | |
Transmembranei | 218 – 227 | Beta strandedBy similarity | 10 | |
Transmembranei | 231 – 238 | Beta strandedBy similarity | 8 | |
Transmembranei | 242 – 251 | Beta strandedBy similarity | 10 | |
Transmembranei | 254 – 263 | Beta strandedBy similarity | 10 | |
Transmembranei | 273 – 282 | Beta strandedBy similarity | 10 |
Keywords - Cellular componenti
Cell membrane, Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 137 | S → E: Channel conformation is shifted toward the open state. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000050503 | 2 – 283 | Voltage-dependent anion-selective channel protein 1Add BLAST | 282 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 13 | Phosphoserine1 Publication | 1 | |
Modified residuei | 19 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 20 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 20 | N6-succinyllysine; alternateBy similarity | 1 | |
Cross-linki | 20 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Cross-linki | 53 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 67 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 107 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 109 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 109 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Cross-linki | 110 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 137 | Phosphoserine1 Publication | 1 | |
Cross-linki | 161 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 193 | Phosphoserine; by NEK1By similarity | 1 | |
Modified residuei | 240 | PhosphoserineBy similarity | 1 | |
Modified residuei | 252 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 266 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 266 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q9Z2L0 |
PaxDbi | Q9Z2L0 |
PRIDEi | Q9Z2L0 |
2D gel databases
World-2DPAGEi | 0004:Q9Z2L0 |
PTM databases
CarbonylDBi | Q9Z2L0 |
iPTMneti | Q9Z2L0 |
PhosphoSitePlusi | Q9Z2L0 |
SwissPalmi | Q9Z2L0 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000006375, Expressed in skeletal muscle tissue and 20 other tissues |
Genevisiblei | Q9Z2L0, RN |
Interactioni
Subunit structurei
Homodimer and homotrimer; in response to cyclic AMP or calcium.
Interacts with hexokinases including HK1. The HK1-VDAC1 complex interacts with ATF2.
Interacts with BCL2L1.
Interacts with BAK1.
Interacts with RTL10/BOP (via BH3 domain).
Interacts with amyloid-beta and APP; induces VDAC1 dephosphorylation.
Component of the mitochondrial permeability transition pore complex (mPTPC), at least composed of SPG7, VDAC1 and PPIF.
Interacts with SPG7, NIPSNAP2 and SLC25A30.
Interacts with TMEM41B.
Interacts with BCAP31.
By similarityGO - Molecular functioni
- identical protein binding Source: Ensembl
- ion channel binding Source: RGD
- protein kinase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 249746, 7 interactors |
CORUMi | Q9Z2L0 |
DIPi | DIP-37068N |
IntActi | Q9Z2L0, 8 interactors |
MINTi | Q9Z2L0 |
STRINGi | 10116.ENSRNOP00000008477 |
Family & Domainsi
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane beta strandPhylogenomic databases
eggNOGi | KOG3126, Eukaryota |
GeneTreei | ENSGT00950000182869 |
HOGENOMi | CLU_044399_2_0_1 |
InParanoidi | Q9Z2L0 |
OMAi | VDYDING |
OrthoDBi | 938262at2759 |
PhylomeDBi | Q9Z2L0 |
TreeFami | TF315091 |
Family and domain databases
Gene3Di | 2.40.160.10, 1 hit |
InterProi | View protein in InterPro IPR023614, Porin_dom_sf IPR001925, Porin_Euk IPR027246, Porin_Euk/Tom40 IPR030270, VDAC1 |
PANTHERi | PTHR11743, PTHR11743, 1 hit PTHR11743:SF13, PTHR11743:SF13, 1 hit |
Pfami | View protein in Pfam PF01459, Porin_3, 1 hit |
PRINTSi | PR00185, EUKARYTPORIN |
PROSITEi | View protein in PROSITE PS00558, EUKARYOTIC_PORIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAVPPTYADL GKSARDVFTK GYGFGLIKLD LKTKSENGLE FTSSGSANTE
60 70 80 90 100
TTKVNGSLET KYRWTEYGLT FTEKWNTDNT LGTEITVEDQ LARGLKLTFD
110 120 130 140 150
SSFSPNTGKK NAKIKTGYKR EHINLGCDVD FDIAGPSIRG ALVLGYEGWL
160 170 180 190 200
AGYQMNFETS KSRVTQSNFA VGYKTDEFQL HTNVNDGTEF GGSIYQKVNK
210 220 230 240 250
KLETAVNLAW TAGNSNTRFG IAAKYQVDPD ACFSAKVNNS SLIGLGYTQT
260 270 280
LKPGIKLTLS ALLDGKNVNA GGHKLGLGLE FQA
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 55 | N → T in AAD02476 (PubMed:9714728).Curated | 1 | |
Sequence conflicti | 83 | T → S in AAD02476 (PubMed:9714728).Curated | 1 | |
Sequence conflicti | 129 | V → M in AAD02476 (PubMed:9714728).Curated | 1 | |
Sequence conflicti | 148 | G → GFDIAGPSIRGALVLGYEG in AAD02476 (PubMed:9714728).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF048828 mRNA Translation: AAD02476.1 AB039662 mRNA Translation: BAB13473.1 AF268467 mRNA Translation: AAF80115.1 BC060558 mRNA Translation: AAH60558.1 Different initiation. BC072484 mRNA Translation: AAH72484.2 BC087573 mRNA Translation: AAH87573.2 BC087657 mRNA Translation: AAH87657.2 BC104684 mRNA Translation: AAI04685.2 BC127491 mRNA Translation: AAI27492.1 |
RefSeqi | NP_112643.1, NM_031353.1 |
Genome annotation databases
Ensembli | ENSRNOT00000008477; ENSRNOP00000008477; ENSRNOG00000006375 |
GeneIDi | 83529 |
KEGGi | rno:83529 |
UCSCi | RGD:621575, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF048828 mRNA Translation: AAD02476.1 AB039662 mRNA Translation: BAB13473.1 AF268467 mRNA Translation: AAF80115.1 BC060558 mRNA Translation: AAH60558.1 Different initiation. BC072484 mRNA Translation: AAH72484.2 BC087573 mRNA Translation: AAH87573.2 BC087657 mRNA Translation: AAH87657.2 BC104684 mRNA Translation: AAI04685.2 BC127491 mRNA Translation: AAI27492.1 |
RefSeqi | NP_112643.1, NM_031353.1 |
3D structure databases
SMRi | Q9Z2L0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 249746, 7 interactors |
CORUMi | Q9Z2L0 |
DIPi | DIP-37068N |
IntActi | Q9Z2L0, 8 interactors |
MINTi | Q9Z2L0 |
STRINGi | 10116.ENSRNOP00000008477 |
PTM databases
CarbonylDBi | Q9Z2L0 |
iPTMneti | Q9Z2L0 |
PhosphoSitePlusi | Q9Z2L0 |
SwissPalmi | Q9Z2L0 |
2D gel databases
World-2DPAGEi | 0004:Q9Z2L0 |
Proteomic databases
jPOSTi | Q9Z2L0 |
PaxDbi | Q9Z2L0 |
PRIDEi | Q9Z2L0 |
Genome annotation databases
Ensembli | ENSRNOT00000008477; ENSRNOP00000008477; ENSRNOG00000006375 |
GeneIDi | 83529 |
KEGGi | rno:83529 |
UCSCi | RGD:621575, rat |
Organism-specific databases
CTDi | 7416 |
RGDi | 621575, Vdac1 |
Phylogenomic databases
eggNOGi | KOG3126, Eukaryota |
GeneTreei | ENSGT00950000182869 |
HOGENOMi | CLU_044399_2_0_1 |
InParanoidi | Q9Z2L0 |
OMAi | VDYDING |
OrthoDBi | 938262at2759 |
PhylomeDBi | Q9Z2L0 |
TreeFami | TF315091 |
Enzyme and pathway databases
Reactomei | R-RNO-5205685, PINK1-PRKN Mediated Mitophagy R-RNO-5689880, Ub-specific processing proteases R-RNO-70268, Pyruvate metabolism |
Miscellaneous databases
PROi | PR:Q9Z2L0 |
Gene expression databases
Bgeei | ENSRNOG00000006375, Expressed in skeletal muscle tissue and 20 other tissues |
Genevisiblei | Q9Z2L0, RN |
Family and domain databases
Gene3Di | 2.40.160.10, 1 hit |
InterProi | View protein in InterPro IPR023614, Porin_dom_sf IPR001925, Porin_Euk IPR027246, Porin_Euk/Tom40 IPR030270, VDAC1 |
PANTHERi | PTHR11743, PTHR11743, 1 hit PTHR11743:SF13, PTHR11743:SF13, 1 hit |
Pfami | View protein in Pfam PF01459, Porin_3, 1 hit |
PRINTSi | PR00185, EUKARYTPORIN |
PROSITEi | View protein in PROSITE PS00558, EUKARYOTIC_PORIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VDAC1_RAT | |
Accessioni | Q9Z2L0Primary (citable) accession number: Q9Z2L0 Secondary accession number(s): A1L125 Q6P9W9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 152 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families