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Protein

Methyl-CpG-binding domain protein 2

Gene

Mbd2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression (By similarity). May enhance the activation of some unmethylated cAMP-responsive promoters (By similarity). Selectively represses transcription activity of methylated rRNA promoters.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-427413 NoRC negatively regulates rRNA expression
R-MMU-73728 RNA Polymerase I Promoter Opening

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-CpG-binding domain protein 2
Alternative name(s):
Methyl-CpG-binding protein MBD2
Gene namesi
Name:Mbd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1333813 Mbd2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000962611 – 414Methyl-CpG-binding domain protein 2Add BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei184PhosphoserineBy similarity1
Modified residuei410PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Z2E1
PaxDbiQ9Z2E1
PeptideAtlasiQ9Z2E1
PRIDEiQ9Z2E1

PTM databases

iPTMnetiQ9Z2E1
PhosphoSitePlusiQ9Z2E1

Expressioni

Tissue specificityi

Highly expressed in brain, heart, kidney, lung, skeletal muscle, spleen and testis. Detected at lower levels in embryonic stem cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000024513 Expressed in 328 organ(s), highest expression level in blood
CleanExiMM_MBD2
ExpressionAtlasiQ9Z2E1 baseline and differential
GenevisibleiQ9Z2E1 MM

Interactioni

Subunit structurei

Heterodimer with MBD3. Component of the MeCP1 complex that contains HDAC1 and HDAC2. Binds DNMT1, MIZF, GPN1, SIN3A, GATAD2A/p66-alpha and GATAD2B/p66-beta (By similarity). Interacts with DHX9 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201331, 25 interactors
ComplexPortaliCPX-953 MBD2/NuRD nucleosome remodeling and deacetylase complex
CORUMiQ9Z2E1
IntActiQ9Z2E1, 24 interactors
MINTiQ9Z2E1
STRINGi10090.ENSMUSP00000073701

Structurei

3D structure databases

ProteinModelPortaliQ9Z2E1
SMRiQ9Z2E1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 216MBDPROSITE-ProRule annotationAdd BLAST69

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi6 – 143Gly-richAdd BLAST138
Compositional biasi50 – 96Arg-richAdd BLAST47

Phylogenomic databases

eggNOGiENOG410INB9 Eukaryota
ENOG410ZPAZ LUCA
GeneTreeiENSGT00410000025376
HOGENOMiHOG000013073
HOVERGENiHBG052417
InParanoidiQ9Z2E1
KOiK11590
OMAiGAMERKR
OrthoDBiEOG091G0I05
TreeFamiTF325032

Family and domain databases

InterProiView protein in InterPro
IPR016177 DNA-bd_dom_sf
IPR032343 MBD2/MBD3_p55-bd
IPR025884 MeCpG-bd_2/3_C_dom
IPR001739 Methyl_CpG_DNA-bd
PfamiView protein in Pfam
PF01429 MBD, 1 hit
PF14048 MBD_C, 1 hit
PF16564 MBDa, 1 hit
SMARTiView protein in SMART
SM00391 MBD, 1 hit
SUPFAMiSSF54171 SSF54171, 1 hit
PROSITEiView protein in PROSITE
PS50982 MBD, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Z2E1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRAHPGGGRC CPEQEEGESA AGGSGAGGDS AIEQGGQGSA LAPSPVSGVR
60 70 80 90 100
REGARGGGRG RGRWKQAARG GGVCGRGRGR GRGRGRGRGR GRGRGRPQSG
110 120 130 140 150
GSGLGGDGGG GAGGCGGGSG GGVAPRRDPV PFPSGSSGPG PRGPRATESG
160 170 180 190 200
KRMDCPALPP GWKKEEVIRK SGLSAGKSDV YYFSPSGKKF RSKPQLARYL
210 220 230 240 250
GNAVDLSSFD FRTGKMMPSK LQKNKQRLRN DPLNQNKGKP DLNTTLPIRQ
260 270 280 290 300
TASIFKQPVT KFTNHPSNKV KSDPQRMNEQ PRQLFWEKRL QGLSASDVTE
310 320 330 340 350
QIIKTMELPK GLQGVGPGSN DETLLSAVAS ALHTSSAPIT GQVSAAVEKN
360 370 380 390 400
PAVWLNTSQP LCKAFIVTDE DIRKQEERVQ QVRKKLEEAL MADILSRAAD
410
TEEVDIDMDS GDEA
Length:414
Mass (Da):43,501
Last modified:July 27, 2011 - v2
Checksum:i1C658D4A6066602A
GO
Isoform 2 (identifier: Q9Z2E1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     238-249: GKPDLNTTLPIR → FRLIKKQTLIGL
     250-414: Missing.

Show »
Length:249
Mass (Da):25,451
Checksum:i530D600B81F98FF0
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6UPR7F6UPR7_MOUSE
Methyl-CpG-binding domain protein 2
Mbd2
72Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti117G → V in AAC68872 (PubMed:9774669).Curated1
Sequence conflicti117G → V in AAC68874 (PubMed:9774669).Curated1
Sequence conflicti117G → V in AAD50372 (PubMed:10441743).Curated1
Sequence conflicti117G → V in AAD50373 (PubMed:10441743).Curated1
Sequence conflicti117G → V in AAH46607 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011079238 – 249GKPDL…TLPIR → FRLIKKQTLIGL in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_011080250 – 414Missing in isoform 2. 1 PublicationAdd BLAST165

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072243 mRNA Translation: AAC68872.1
AF072245 mRNA Translation: AAC68874.1
AF120986
, AF120983, AF120984, AF120985 Genomic DNA Translation: AAD50372.1
AF120983 Genomic DNA Translation: AAD50373.1
AC134831 Genomic DNA No translation available.
AC166815 Genomic DNA No translation available.
AC170591 Genomic DNA No translation available.
BC046607 mRNA Translation: AAH46607.2
CCDSiCCDS29335.1 [Q9Z2E1-1]
CCDS79661.1 [Q9Z2E1-2]
RefSeqiNP_001298000.1, NM_001311071.1 [Q9Z2E1-2]
NP_034903.2, NM_010773.2 [Q9Z2E1-1]
UniGeneiMm.322

Genome annotation databases

EnsembliENSMUST00000074058; ENSMUSP00000073701; ENSMUSG00000024513 [Q9Z2E1-1]
ENSMUST00000114946; ENSMUSP00000110596; ENSMUSG00000024513 [Q9Z2E1-2]
GeneIDi17191
KEGGimmu:17191
UCSCiuc008fom.2 mouse [Q9Z2E1-2]
uc008fon.2 mouse [Q9Z2E1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072243 mRNA Translation: AAC68872.1
AF072245 mRNA Translation: AAC68874.1
AF120986
, AF120983, AF120984, AF120985 Genomic DNA Translation: AAD50372.1
AF120983 Genomic DNA Translation: AAD50373.1
AC134831 Genomic DNA No translation available.
AC166815 Genomic DNA No translation available.
AC170591 Genomic DNA No translation available.
BC046607 mRNA Translation: AAH46607.2
CCDSiCCDS29335.1 [Q9Z2E1-1]
CCDS79661.1 [Q9Z2E1-2]
RefSeqiNP_001298000.1, NM_001311071.1 [Q9Z2E1-2]
NP_034903.2, NM_010773.2 [Q9Z2E1-1]
UniGeneiMm.322

3D structure databases

ProteinModelPortaliQ9Z2E1
SMRiQ9Z2E1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201331, 25 interactors
ComplexPortaliCPX-953 MBD2/NuRD nucleosome remodeling and deacetylase complex
CORUMiQ9Z2E1
IntActiQ9Z2E1, 24 interactors
MINTiQ9Z2E1
STRINGi10090.ENSMUSP00000073701

PTM databases

iPTMnetiQ9Z2E1
PhosphoSitePlusiQ9Z2E1

Proteomic databases

EPDiQ9Z2E1
PaxDbiQ9Z2E1
PeptideAtlasiQ9Z2E1
PRIDEiQ9Z2E1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074058; ENSMUSP00000073701; ENSMUSG00000024513 [Q9Z2E1-1]
ENSMUST00000114946; ENSMUSP00000110596; ENSMUSG00000024513 [Q9Z2E1-2]
GeneIDi17191
KEGGimmu:17191
UCSCiuc008fom.2 mouse [Q9Z2E1-2]
uc008fon.2 mouse [Q9Z2E1-1]

Organism-specific databases

CTDi8932
MGIiMGI:1333813 Mbd2

Phylogenomic databases

eggNOGiENOG410INB9 Eukaryota
ENOG410ZPAZ LUCA
GeneTreeiENSGT00410000025376
HOGENOMiHOG000013073
HOVERGENiHBG052417
InParanoidiQ9Z2E1
KOiK11590
OMAiGAMERKR
OrthoDBiEOG091G0I05
TreeFamiTF325032

Enzyme and pathway databases

ReactomeiR-MMU-427413 NoRC negatively regulates rRNA expression
R-MMU-73728 RNA Polymerase I Promoter Opening

Miscellaneous databases

ChiTaRSiMbd2 mouse
PROiPR:Q9Z2E1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024513 Expressed in 328 organ(s), highest expression level in blood
CleanExiMM_MBD2
ExpressionAtlasiQ9Z2E1 baseline and differential
GenevisibleiQ9Z2E1 MM

Family and domain databases

InterProiView protein in InterPro
IPR016177 DNA-bd_dom_sf
IPR032343 MBD2/MBD3_p55-bd
IPR025884 MeCpG-bd_2/3_C_dom
IPR001739 Methyl_CpG_DNA-bd
PfamiView protein in Pfam
PF01429 MBD, 1 hit
PF14048 MBD_C, 1 hit
PF16564 MBDa, 1 hit
SMARTiView protein in SMART
SM00391 MBD, 1 hit
SUPFAMiSSF54171 SSF54171, 1 hit
PROSITEiView protein in PROSITE
PS50982 MBD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMBD2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2E1
Secondary accession number(s): E9QMV9, Q811D9, Q9Z2D9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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