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Protein

Gasdermin-E

Gene

Gsdme

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53.By similarity
Gasdermin-E, N-terminal: Switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis. Produced by the cleavage of GSDME by CASP3, perforates cell membrane and thereby induces pyroptosis. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate (By similarity). Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents (PubMed:28045099). Exhibits bactericidal activity (By similarity).By similarity1 Publication

GO - Molecular functioni

  • cardiolipin binding Source: UniProtKB
  • phosphatidylinositol-4,5-bisphosphate binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processNecrosis

Names & Taxonomyi

Protein namesi
Recommended name:
Gasdermin-EBy similarity
Alternative name(s):
Non-syndromic hearing impairment protein 5 homologCurated
Cleaved into the following 2 chains:
Gasdermin-E, N-terminalBy similarity
Short name:
GSDME-NTBy similarity
Gasdermin-E, C-terminalBy similarity
Short name:
GSDME-CTBy similarity
Gene namesi
Name:GsdmeBy similarity
Synonyms:Dfna5, Dfna5h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1889850 Gsdme

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice develop normally, including the immune system. After injection of the chemotherapy drug cisplatin, they look more healthy and vigorous than wild type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001481791 – 512Gasdermin-EAdd BLAST512
ChainiPRO_00004427491 – 270Gasdermin-E, N-terminalBy similarityAdd BLAST270
ChainiPRO_0000442750271 – 499Gasdermin-E, C-terminalBy similarityAdd BLAST229

Post-translational modificationi

Cleavage at Asp-270 by CASP3 (mature and uncleaved precursor forms) relieves autoinhibition and is sufficient to initiate pyroptosis.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei270 – 271Cleavage; by CASP3By similarity2

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ9Z2D3
PaxDbiQ9Z2D3
PRIDEiQ9Z2D3

PTM databases

iPTMnetiQ9Z2D3
PhosphoSitePlusiQ9Z2D3

Expressioni

Tissue specificityi

Expressed in spleen, kidney, large and small intestine, testicle, stomach and by CD4+CD8+ T cells in thymus (PubMed:28459430). Expressed by macrophages (PubMed:28045099).2 Publications

Gene expression databases

BgeeiENSMUSG00000029821 Expressed in 280 organ(s), highest expression level in pineal body
ExpressionAtlasiQ9Z2D3 baseline and differential
GenevisibleiQ9Z2D3 MM

Interactioni

Subunit structurei

The N-terminal moiety forms homooligomer; disulfide-linked. May form an 16-mer complex. Oligomerization occurs in the presence of membranes.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031845

Structurei

3D structure databases

ProteinModelPortaliQ9Z2D3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 56Membrane targeting domainBy similarityAdd BLAST56

Domaini

Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain, that is released upon cleavage by CASP3.By similarity

Sequence similaritiesi

Belongs to the gasdermin family.Curated

Phylogenomic databases

eggNOGiENOG410IHRC Eukaryota
ENOG410XTJB LUCA
GeneTreeiENSGT00530000063574
HOGENOMiHOG000065726
HOVERGENiHBG051340
InParanoidiQ9Z2D3
KOiK22146
OrthoDBiEOG091G0948
PhylomeDBiQ9Z2D3
TreeFamiTF352821

Family and domain databases

InterProiView protein in InterPro
IPR007677 Gasdermin
PfamiView protein in Pfam
PF04598 Gasdermin, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9Z2D3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFAKATRNFL KEVDAGGDLI SVSHLNDSDK LQLLSLVTKK KRYWCWQRPK
60 70 80 90 100
YQILSATLED VLTEGHCLSP VVVESDFVKY ESKCENHKSG AIGTVVGKVK
110 120 130 140 150
LNVGGKGVVE SHSSFGTLRK QEVDVQQLIQ DAVKRTVNMD NLVLQQVLES
160 170 180 190 200
RNEVLCVLTQ KIMTTQKCVI SEHVQSEETC GGMVGIQTKT IQVSATEDGT
210 220 230 240 250
VTTDTNVVLE IPAATTIAYG IMELFVKQDG QFEFCLLQGK HGGFEHERKL
260 270 280 290 300
DSVYLDPLAY REFAFLDMLD GGQGISSQDG PLRVVKQATL HLERSFHPFA
310 320 330 340 350
VLPAQQQRAL FCVLQKILFD EELLRALEQV CDDVAGGLWS SQAVLAMEEL
360 370 380 390 400
TDSQQQDLTA FLQLVGYRIQ GEHPGPQDEV SNQKLFATAY FLVSALAEMP
410 420 430 440 450
DNATVFLGTC CKLHVISSLC CLLHALSDDS VCDFHNPTLA PLRDTERFGI
460 470 480 490 500
VQRLFASADI ALERMQFSAK ATILKDSCIF PLILHITLSG LSTLSKEHEE
510
ELCQSGHATG QD
Length:512
Mass (Da):56,631
Last modified:May 1, 1999 - v1
Checksum:i13AFB8627773C4A5
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YYP5D3YYP5_MOUSE
Gasdermin-E
Gsdme Dfna5
399Annotation score:
E9Q308E9Q308_MOUSE
Gasdermin-E
Gsdme Dfna5
123Annotation score:
E9Q5V3E9Q5V3_MOUSE
Gasdermin-E
Gsdme Dfna5
424Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073309 mRNA Translation: AAC69325.1
AK016561 mRNA Translation: BAB30305.1
CCDSiCCDS20130.1
RefSeqiNP_061239.1, NM_018769.3
UniGeneiMm.248361

Genome annotation databases

EnsembliENSMUST00000031845; ENSMUSP00000031845; ENSMUSG00000029821
ENSMUST00000170142; ENSMUSP00000126759; ENSMUSG00000029821
GeneIDi54722
KEGGimmu:54722
UCSCiuc009bwx.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073309 mRNA Translation: AAC69325.1
AK016561 mRNA Translation: BAB30305.1
CCDSiCCDS20130.1
RefSeqiNP_061239.1, NM_018769.3
UniGeneiMm.248361

3D structure databases

ProteinModelPortaliQ9Z2D3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031845

PTM databases

iPTMnetiQ9Z2D3
PhosphoSitePlusiQ9Z2D3

Proteomic databases

MaxQBiQ9Z2D3
PaxDbiQ9Z2D3
PRIDEiQ9Z2D3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031845; ENSMUSP00000031845; ENSMUSG00000029821
ENSMUST00000170142; ENSMUSP00000126759; ENSMUSG00000029821
GeneIDi54722
KEGGimmu:54722
UCSCiuc009bwx.1 mouse

Organism-specific databases

CTDi1687
MGIiMGI:1889850 Gsdme

Phylogenomic databases

eggNOGiENOG410IHRC Eukaryota
ENOG410XTJB LUCA
GeneTreeiENSGT00530000063574
HOGENOMiHOG000065726
HOVERGENiHBG051340
InParanoidiQ9Z2D3
KOiK22146
OrthoDBiEOG091G0948
PhylomeDBiQ9Z2D3
TreeFamiTF352821

Miscellaneous databases

PROiPR:Q9Z2D3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029821 Expressed in 280 organ(s), highest expression level in pineal body
ExpressionAtlasiQ9Z2D3 baseline and differential
GenevisibleiQ9Z2D3 MM

Family and domain databases

InterProiView protein in InterPro
IPR007677 Gasdermin
PfamiView protein in Pfam
PF04598 Gasdermin, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGSDME_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2D3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 1999
Last modified: November 7, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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