Cldn8

Functionⁱ

Tight-junction protein required for paracellular chloride transport in the kidney (PubMed:20921420, PubMed:25831548). Mediates recruitment of CLDN4 to tight junction in the kidney (PubMed:20921420, PubMed:25831548). Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.2 Publications

GO - Molecular functionⁱ

identical protein binding Source: UniProtKB
structural molecule activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB

View the complete GO annotation on QuickGO ...

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Claudin-82 Publications
Gene namesⁱ	Name:Cldn8Imported
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 16

Organism-specific databases

MGIⁱ	MGI:1859286 Cldn8

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 7	CytoplasmicSequence analysis	7
Transmembraneⁱ	8 – 28	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	29 – 81	ExtracellularSequence analysisAdd BLAST	53
Transmembraneⁱ	82 – 102	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	103 – 117	CytoplasmicSequence analysisAdd BLAST	15
Transmembraneⁱ	118 – 138	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	139 – 166	ExtracellularSequence analysisAdd BLAST	28
Transmembraneⁱ	167 – 187	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	188 – 225	CytoplasmicSequence analysisAdd BLAST	38

Keywords - Cellular componentⁱ

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Conditional knockout mice lacking Cldn8 in the collecting duct of kidney show hypotension, hypokalemia, and metabolic alkalosis (PubMed:25831548).1 Publication

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	213	K → R: Resistant to KLHL3-dependent protein degradation. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000144754	1 – 225	Claudin-8Add BLAST		225

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Cross-linkⁱ	213	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationⁱ

Ubiquitinated by the BCR(KLHL3) E3 ubiquitin ligase complex in the kidney, leading to its degradation.1 Publication

Keywords - PTMⁱ

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q9Z260
PRIDEⁱ	Q9Z260

PTM databases

iPTMnetⁱ	Q9Z260
PhosphoSitePlusⁱ	Q9Z260

Expressionⁱ

Tissue specificityⁱ

Expressed primarily in lung and kidney (PubMed:9892664). Present in both cortical and medullar collecting ducts (at protein level) (PubMed:20921420).2 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000050520 Expressed in 150 organ(s), highest expression level in urinary bladder urothelium
CleanExⁱ	MM_CLDN8
ExpressionAtlasⁱ	Q9Z260 baseline and differential
Genevisibleⁱ	Q9Z260 MM

Interactionⁱ

Subunit structureⁱ

Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (PubMed:10601346). Interacts with CLDN4 (PubMed:20921420). Interacts with KLHL3 (PubMed:25831548).3 Publications

GO - Molecular functionⁱ

identical protein binding Source: UniProtKB

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

Molecular INTeraction database More...MINTⁱ	Q9Z260
STRINGⁱ	10090.ENSMUSP00000051887

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9Z260
SMRⁱ	Q9Z260
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	224 – 225	Interactions with TJP1, TJP2 and TJP31 Publication		2

Sequence similaritiesⁱ

Belongs to the claudin family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IJK4 Eukaryota ENOG410YQQY LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118928
HOGENOMⁱ	HOG000220937
HOVERGENⁱ	HBG000643
InParanoidⁱ	Q9Z260
KEGG Orthology (KO) More...KOⁱ	K06087
OMAⁱ	VTIMPQW
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0MX2
PhylomeDBⁱ	Q9Z260
TreeFamⁱ	TF331936

Family and domain databases

InterProⁱ	View protein in InterPro IPR006187 Claudin IPR003926 Claudin8 IPR017974 Claudin_CS IPR004031 PMP22/EMP/MP20/Claudin
PANTHERⁱ	PTHR12002 PTHR12002, 1 hit PTHR12002:SF24 PTHR12002:SF24, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00822 PMP22_Claudin, 1 hit
PROSITEⁱ	View protein in PROSITE PS01346 CLAUDIN, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9Z260-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MATYALQMAA LVLGGVGMVG TVAVTIMPQW RVSAFIESNI VVFENRWEGL 
        60         70         80         90        100
WMNCMRHANI RMQCKVYDSL LALSPDLQAS RGLMCAASVL AFLAFMTAIL 
       110        120        130        140        150
GMKCTRCTGD DENVKSRILL TAGIIFFITG LVVLIPVSWV ANSIIRDFYN 
       160        170        180        190        200
PLVDVALKRE LGEALYIGWT TALVLIAGGA LFCCVFCCTE RSNSYRYSVP 
       210        220 
SHRTTQRSFH AEKRSPSIYS KSQYV

Length:225

Mass (Da):24,947

Last modified:May 1, 1999 - v1

Checksum:ⁱ12BB3C460F23D876

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF087826 mRNA Translation: AAD09761.1 BC003868 mRNA Translation: AAH03868.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS28296.1
NCBI Reference Sequences More...RefSeqⁱ	NP_061248.1, NM_018778.3
UniGeneⁱ	Mm.25836

Genome annotation databases

Ensemblⁱ	ENSMUST00000049697; ENSMUSP00000051887; ENSMUSG00000050520
GeneIDⁱ	54420
KEGGⁱ	mmu:54420
UCSC genome browser More...UCSCⁱ	uc007zuz.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9Z260	Claudin		MOUSE		225	UniRef100_Q9Z260

Protein	Similar proteins		Species	Score	Length	Source
Q9Z260	Claudin		MOUSE		225	UniRef90_Q9Z260
Claudin		RAT		225
Claudin		MESAU		225
Claudin		CRIGR		225
Claudin (Fragment)		NEOLE		183
+8

Protein	Similar proteins		Species	Score	Length	Source
Q9Z260	Claudin-8		HUMAN		225	UniRef50_Q9Z260
Claudin		MOUSE		225
Claudin		MESAU		225
Claudin		CRIGR		225
Claudin		RAT		225
+178

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF087826 mRNA Translation: AAD09761.1 BC003868 mRNA Translation: AAH03868.1
CCDSⁱ	CCDS28296.1
RefSeqⁱ	NP_061248.1, NM_018778.3
UniGeneⁱ	Mm.25836

3D structure databases

ProteinModelPortalⁱ	Q9Z260
SMRⁱ	Q9Z260
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

MINTⁱ	Q9Z260
STRINGⁱ	10090.ENSMUSP00000051887

PTM databases

iPTMnetⁱ	Q9Z260
PhosphoSitePlusⁱ	Q9Z260

Proteomic databases

PaxDbⁱ	Q9Z260
PRIDEⁱ	Q9Z260

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000049697; ENSMUSP00000051887; ENSMUSG00000050520
GeneIDⁱ	54420
KEGGⁱ	mmu:54420
UCSCⁱ	uc007zuz.2 mouse

Organism-specific databases

CTDⁱ	9073
MGIⁱ	MGI:1859286 Cldn8

Phylogenomic databases

eggNOGⁱ	ENOG410IJK4 Eukaryota ENOG410YQQY LUCA
GeneTreeⁱ	ENSGT00760000118928
HOGENOMⁱ	HOG000220937
HOVERGENⁱ	HBG000643
InParanoidⁱ	Q9Z260
KOⁱ	K06087
OMAⁱ	VTIMPQW
OrthoDBⁱ	EOG091G0MX2
PhylomeDBⁱ	Q9Z260
TreeFamⁱ	TF331936

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9Z260
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000050520 Expressed in 150 organ(s), highest expression level in urinary bladder urothelium
CleanExⁱ	MM_CLDN8
ExpressionAtlasⁱ	Q9Z260 baseline and differential
Genevisibleⁱ	Q9Z260 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR006187 Claudin IPR003926 Claudin8 IPR017974 Claudin_CS IPR004031 PMP22/EMP/MP20/Claudin
PANTHERⁱ	PTHR12002 PTHR12002, 1 hit PTHR12002:SF24 PTHR12002:SF24, 1 hit
Pfamⁱ	View protein in Pfam PF00822 PMP22_Claudin, 1 hit
PROSITEⁱ	View protein in PROSITE PS01346 CLAUDIN, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CLD8_MOUSE
Accessionⁱ	Q9Z260Primary (citable) accession number: Q9Z260
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
	Last sequence update:	May 1, 1999
	Last modified:	November 7, 2018
	This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

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UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9Z260 (CLD8_MOUSE)

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Claudin-8

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Other locations

Plasma Membrane

Other locations

Topology

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ