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Protein

Claudin-8

Gene

Cldn8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tight-junction protein required for paracellular chloride transport in the kidney (PubMed:20921420, PubMed:25831548). Mediates recruitment of CLDN4 to tight junction in the kidney (PubMed:20921420, PubMed:25831548). Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.2 Publications

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Claudin-82 Publications
Gene namesi
Name:Cldn8Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1859286 Cldn8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28HelicalSequence analysisAdd BLAST21
Topological domaini29 – 81ExtracellularSequence analysisAdd BLAST53
Transmembranei82 – 102HelicalSequence analysisAdd BLAST21
Topological domaini103 – 117CytoplasmicSequence analysisAdd BLAST15
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
Topological domaini139 – 166ExtracellularSequence analysisAdd BLAST28
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Topological domaini188 – 225CytoplasmicSequence analysisAdd BLAST38

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Disruption phenotypei

Conditional knockout mice lacking Cldn8 in the collecting duct of kidney show hypotension, hypokalemia, and metabolic alkalosis (PubMed:25831548).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi213K → R: Resistant to KLHL3-dependent protein degradation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001447541 – 225Claudin-8Add BLAST225

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Ubiquitinated by the BCR(KLHL3) E3 ubiquitin ligase complex in the kidney, leading to its degradation.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9Z260
PRIDEiQ9Z260

PTM databases

iPTMnetiQ9Z260
PhosphoSitePlusiQ9Z260

Expressioni

Tissue specificityi

Expressed primarily in lung and kidney (PubMed:9892664). Present in both cortical and medullar collecting ducts (at protein level) (PubMed:20921420).2 Publications

Gene expression databases

BgeeiENSMUSG00000050520 Expressed in 150 organ(s), highest expression level in urinary bladder urothelium
CleanExiMM_CLDN8
ExpressionAtlasiQ9Z260 baseline and differential
GenevisibleiQ9Z260 MM

Interactioni

Subunit structurei

Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (PubMed:10601346). Interacts with CLDN4 (PubMed:20921420). Interacts with KLHL3 (PubMed:25831548).3 Publications

GO - Molecular functioni

Protein-protein interaction databases

MINTiQ9Z260
STRINGi10090.ENSMUSP00000051887

Structurei

3D structure databases

ProteinModelPortaliQ9Z260
SMRiQ9Z260
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni224 – 225Interactions with TJP1, TJP2 and TJP31 Publication2

Sequence similaritiesi

Belongs to the claudin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJK4 Eukaryota
ENOG410YQQY LUCA
GeneTreeiENSGT00760000118928
HOGENOMiHOG000220937
HOVERGENiHBG000643
InParanoidiQ9Z260
KOiK06087
OMAiVTIMPQW
OrthoDBiEOG091G0MX2
PhylomeDBiQ9Z260
TreeFamiTF331936

Family and domain databases

InterProiView protein in InterPro
IPR006187 Claudin
IPR003926 Claudin8
IPR017974 Claudin_CS
IPR004031 PMP22/EMP/MP20/Claudin
PANTHERiPTHR12002 PTHR12002, 1 hit
PTHR12002:SF24 PTHR12002:SF24, 1 hit
PfamiView protein in Pfam
PF00822 PMP22_Claudin, 1 hit
PROSITEiView protein in PROSITE
PS01346 CLAUDIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z260-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATYALQMAA LVLGGVGMVG TVAVTIMPQW RVSAFIESNI VVFENRWEGL
60 70 80 90 100
WMNCMRHANI RMQCKVYDSL LALSPDLQAS RGLMCAASVL AFLAFMTAIL
110 120 130 140 150
GMKCTRCTGD DENVKSRILL TAGIIFFITG LVVLIPVSWV ANSIIRDFYN
160 170 180 190 200
PLVDVALKRE LGEALYIGWT TALVLIAGGA LFCCVFCCTE RSNSYRYSVP
210 220
SHRTTQRSFH AEKRSPSIYS KSQYV
Length:225
Mass (Da):24,947
Last modified:May 1, 1999 - v1
Checksum:i12BB3C460F23D876
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087826 mRNA Translation: AAD09761.1
BC003868 mRNA Translation: AAH03868.1
CCDSiCCDS28296.1
RefSeqiNP_061248.1, NM_018778.3
UniGeneiMm.25836

Genome annotation databases

EnsembliENSMUST00000049697; ENSMUSP00000051887; ENSMUSG00000050520
GeneIDi54420
KEGGimmu:54420
UCSCiuc007zuz.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087826 mRNA Translation: AAD09761.1
BC003868 mRNA Translation: AAH03868.1
CCDSiCCDS28296.1
RefSeqiNP_061248.1, NM_018778.3
UniGeneiMm.25836

3D structure databases

ProteinModelPortaliQ9Z260
SMRiQ9Z260
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiQ9Z260
STRINGi10090.ENSMUSP00000051887

PTM databases

iPTMnetiQ9Z260
PhosphoSitePlusiQ9Z260

Proteomic databases

PaxDbiQ9Z260
PRIDEiQ9Z260

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049697; ENSMUSP00000051887; ENSMUSG00000050520
GeneIDi54420
KEGGimmu:54420
UCSCiuc007zuz.2 mouse

Organism-specific databases

CTDi9073
MGIiMGI:1859286 Cldn8

Phylogenomic databases

eggNOGiENOG410IJK4 Eukaryota
ENOG410YQQY LUCA
GeneTreeiENSGT00760000118928
HOGENOMiHOG000220937
HOVERGENiHBG000643
InParanoidiQ9Z260
KOiK06087
OMAiVTIMPQW
OrthoDBiEOG091G0MX2
PhylomeDBiQ9Z260
TreeFamiTF331936

Miscellaneous databases

PROiPR:Q9Z260
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050520 Expressed in 150 organ(s), highest expression level in urinary bladder urothelium
CleanExiMM_CLDN8
ExpressionAtlasiQ9Z260 baseline and differential
GenevisibleiQ9Z260 MM

Family and domain databases

InterProiView protein in InterPro
IPR006187 Claudin
IPR003926 Claudin8
IPR017974 Claudin_CS
IPR004031 PMP22/EMP/MP20/Claudin
PANTHERiPTHR12002 PTHR12002, 1 hit
PTHR12002:SF24 PTHR12002:SF24, 1 hit
PfamiView protein in Pfam
PF00822 PMP22_Claudin, 1 hit
PROSITEiView protein in PROSITE
PS01346 CLAUDIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCLD8_MOUSE
AccessioniPrimary (citable) accession number: Q9Z260
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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