UniProtKB - Q9Z260 (CLD8_MOUSE)
Protein
Claudin-8
Gene
Cldn8
Organism
Mus musculus (Mouse)
Status
Functioni
Tight-junction protein required for paracellular chloride transport in the kidney (PubMed:20921420, PubMed:25831548). Mediates recruitment of CLDN4 to tight junction in the kidney (PubMed:20921420, PubMed:25831548). Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.2 Publications
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- structural molecule activity Source: InterPro
GO - Biological processi
- bicellular tight junction assembly Source: GO_Central
- calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
- cell adhesion Source: GO_Central
Names & Taxonomyi
Protein namesi | Recommended name: Claudin-82 Publications |
Gene namesi | Name:Cldn8Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1859286, Cldn8 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Multi-pass membrane protein Sequence analysis
Other locations
- tight junction 3 Publications
Plasma Membrane
- apicolateral plasma membrane Source: UniProtKB
- basolateral plasma membrane Source: MGI
- plasma membrane Source: GO_Central
Other locations
- bicellular tight junction Source: UniProtKB
- integral component of membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 7 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 8 – 28 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 29 – 81 | ExtracellularSequence analysisAdd BLAST | 53 | |
Transmembranei | 82 – 102 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 103 – 117 | CytoplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 118 – 138 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 139 – 166 | ExtracellularSequence analysisAdd BLAST | 28 | |
Transmembranei | 167 – 187 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 188 – 225 | CytoplasmicSequence analysisAdd BLAST | 38 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Tight junctionPathology & Biotechi
Disruption phenotypei
Conditional knockout mice lacking Cldn8 in the collecting duct of kidney show hypotension, hypokalemia, and metabolic alkalosis (PubMed:25831548).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 213 | K → R: Resistant to KLHL3-dependent protein degradation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144754 | 1 – 225 | Claudin-8Add BLAST | 225 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 213 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication |
Post-translational modificationi
Ubiquitinated by the BCR(KLHL3) E3 ubiquitin ligase complex in the kidney, leading to its degradation.1 Publication
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | Q9Z260 |
PRIDEi | Q9Z260 |
PTM databases
iPTMneti | Q9Z260 |
PhosphoSitePlusi | Q9Z260 |
Expressioni
Tissue specificityi
Expressed primarily in lung and kidney (PubMed:9892664). Present in both cortical and medullar collecting ducts (at protein level) (PubMed:20921420).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000050520, Expressed in urinary bladder urothelium and 171 other tissues |
ExpressionAtlasi | Q9Z260, baseline and differential |
Genevisiblei | Q9Z260, MM |
Interactioni
Subunit structurei
GO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
MINTi | Q9Z260 |
STRINGi | 10090.ENSMUSP00000051887 |
Miscellaneous databases
RNActi | Q9Z260, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 224 – 225 | Interactions with TJP1, TJP2 and TJP31 Publication | 2 |
Sequence similaritiesi
Belongs to the claudin family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502RTNJ, Eukaryota |
GeneTreei | ENSGT00940000159077 |
HOGENOMi | CLU_076370_1_2_1 |
InParanoidi | Q9Z260 |
OMAi | CMRHANI |
OrthoDBi | 1314055at2759 |
PhylomeDBi | Q9Z260 |
TreeFami | TF331936 |
Family and domain databases
InterProi | View protein in InterPro IPR006187, Claudin IPR003926, Claudin8 IPR017974, Claudin_CS IPR004031, PMP22/EMP/MP20/Claudin |
PANTHERi | PTHR12002, PTHR12002, 1 hit PTHR12002:SF24, PTHR12002:SF24, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PROSITEi | View protein in PROSITE PS01346, CLAUDIN, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9Z260-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATYALQMAA LVLGGVGMVG TVAVTIMPQW RVSAFIESNI VVFENRWEGL
60 70 80 90 100
WMNCMRHANI RMQCKVYDSL LALSPDLQAS RGLMCAASVL AFLAFMTAIL
110 120 130 140 150
GMKCTRCTGD DENVKSRILL TAGIIFFITG LVVLIPVSWV ANSIIRDFYN
160 170 180 190 200
PLVDVALKRE LGEALYIGWT TALVLIAGGA LFCCVFCCTE RSNSYRYSVP
210 220
SHRTTQRSFH AEKRSPSIYS KSQYV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF087826 mRNA Translation: AAD09761.1 BC003868 mRNA Translation: AAH03868.1 |
CCDSi | CCDS28296.1 |
RefSeqi | NP_061248.1, NM_018778.3 |
Genome annotation databases
Ensembli | ENSMUST00000049697; ENSMUSP00000051887; ENSMUSG00000050520 |
GeneIDi | 54420 |
KEGGi | mmu:54420 |
UCSCi | uc007zuz.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF087826 mRNA Translation: AAD09761.1 BC003868 mRNA Translation: AAH03868.1 |
CCDSi | CCDS28296.1 |
RefSeqi | NP_061248.1, NM_018778.3 |
3D structure databases
SMRi | Q9Z260 |
ModBasei | Search... |
Protein-protein interaction databases
MINTi | Q9Z260 |
STRINGi | 10090.ENSMUSP00000051887 |
PTM databases
iPTMneti | Q9Z260 |
PhosphoSitePlusi | Q9Z260 |
Proteomic databases
PaxDbi | Q9Z260 |
PRIDEi | Q9Z260 |
Protocols and materials databases
Antibodypediai | 6589, 244 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000049697; ENSMUSP00000051887; ENSMUSG00000050520 |
GeneIDi | 54420 |
KEGGi | mmu:54420 |
UCSCi | uc007zuz.2, mouse |
Organism-specific databases
CTDi | 9073 |
MGIi | MGI:1859286, Cldn8 |
Phylogenomic databases
eggNOGi | ENOG502RTNJ, Eukaryota |
GeneTreei | ENSGT00940000159077 |
HOGENOMi | CLU_076370_1_2_1 |
InParanoidi | Q9Z260 |
OMAi | CMRHANI |
OrthoDBi | 1314055at2759 |
PhylomeDBi | Q9Z260 |
TreeFami | TF331936 |
Miscellaneous databases
BioGRID-ORCSi | 54420, 0 hits in 17 CRISPR screens |
ChiTaRSi | Cldn8, mouse |
PROi | PR:Q9Z260 |
RNActi | Q9Z260, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000050520, Expressed in urinary bladder urothelium and 171 other tissues |
ExpressionAtlasi | Q9Z260, baseline and differential |
Genevisiblei | Q9Z260, MM |
Family and domain databases
InterProi | View protein in InterPro IPR006187, Claudin IPR003926, Claudin8 IPR017974, Claudin_CS IPR004031, PMP22/EMP/MP20/Claudin |
PANTHERi | PTHR12002, PTHR12002, 1 hit PTHR12002:SF24, PTHR12002:SF24, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PROSITEi | View protein in PROSITE PS01346, CLAUDIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CLD8_MOUSE | |
Accessioni | Q9Z260Primary (citable) accession number: Q9Z260 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families