UniProtKB - Q9Z214 (HOME1_RAT)
Homer protein homolog 1
Homer1
Functioni
GO - Molecular functioni
- G protein-coupled glutamate receptor binding Source: UniProtKB
- identical protein binding Source: IntAct
- ion channel binding Source: RGD
- molecular adaptor activity Source: BHF-UCL
- protein-containing complex binding Source: RGD
- scaffold protein binding Source: BHF-UCL
- signaling receptor binding Source: RGD
- structural constituent of postsynapse Source: SynGO
- type 5 metabotropic glutamate receptor binding Source: RGD
GO - Biological processi
- behavioral response to cocaine Source: RGD
- chemical homeostasis within a tissue Source: RGD
- circadian rhythm Source: RGD
- G protein-coupled glutamate receptor signaling pathway Source: RGD
- positive regulation of calcium ion transport Source: RGD
- protein localization to synapse Source: BHF-UCL
- protein tetramerization Source: UniProtKB
- regulation of calcium ion import Source: RGD
- regulation of cation channel activity Source: RGD
- regulation of dendritic spine maintenance Source: UniProtKB
- regulation of postsynaptic neurotransmitter receptor activity Source: RGD
- regulation of store-operated calcium entry Source: RGD
- regulation of synaptic transmission, glutamatergic Source: UniProtKB
- response to calcium ion Source: RGD
- response to cocaine Source: RGD
- response to nicotine Source: RGD
- skeletal muscle contraction Source: RGD
- skeletal muscle fiber development Source: RGD
Enzyme and pathway databases
Reactomei | R-RNO-6794361, Neurexins and neuroligins |
Names & Taxonomyi
Protein namesi | Recommended name: Homer protein homolog 1CuratedAlternative name(s): PSD-Zip451 Publication VASP/Ena-related gene up-regulated during seizure and LTP 1 Short name: Vesl-1By similarity |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 628725, Homer1 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- postsynaptic density By similarity
- synapse 1 Publication
- dendritic spine 2 Publications
Note: Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum.By similarity
Cytosol
- cytosol Source: Reactome
- postsynaptic cytosol Source: RGD
Plasma Membrane
- plasma membrane Source: RGD
Other locations
- apical part of cell Source: RGD
- axon Source: RGD
- costamere Source: RGD
- cytoplasm Source: RGD
- dendrite Source: RGD
- dendritic shaft Source: RGD
- dendritic spine Source: UniProtKB
- glutamatergic synapse Source: SynGO
- membrane Source: RGD
- neuron projection Source: RGD
- neuron spine Source: UniProtKB
- neuronal cell body Source: RGD
- postsynapse Source: RGD
- postsynaptic density Source: BHF-UCL
- synapse Source: RGD
- Z disc Source: RGD
Keywords - Cellular componenti
Cell junction, Cell projection, Cytoplasm, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 24 | W → A: Disrupts binding to both GRM1 and SHANK3. Does not form the high-order complex with SHANK1. 2 Publications | 1 | |
Mutagenesisi | 24 | W → Y: Disrupts binding to GRM1. 1 Publication | 1 | |
Mutagenesisi | 70 | T → A: Normal binding. 1 Publication | 1 | |
Mutagenesisi | 70 | T → E: Disrupts binding to SHANK3. 1 Publication | 1 | |
Mutagenesisi | 74 | F → A: Eliminates binding to both GRM1 and SHANK3. 1 Publication | 1 | |
Mutagenesisi | 76 | Q → A: Normal binding. 1 Publication | 1 | |
Mutagenesisi | 76 | Q → R: Normal binding. 1 Publication | 1 | |
Mutagenesisi | 85 | V → A: Diminishes binding to GRM1. 1 Publication | 1 | |
Mutagenesisi | 89 | G → A: Eliminates binding to both GRM1 and SHANK3. 1 Publication | 1 | |
Mutagenesisi | 89 | G → N: Eliminates binding to both GRM1 and SHANK3. 1 Publication | 1 | |
Mutagenesisi | 344 | I → R: Dimeric form; when associated with E-349. Does not form the high-order complex with SHANK1; when associated with E-349. Does not change its interaction with STX12; when associated with E-349. Reduces localization to the spine; when associated with E-349. Affects dendritic spine structure; when associated with E-349. Decreases synaptic transmission; when associated with E-349. Does not affect glutamate receptors ratio; when associated with E-349. 1 Publication | 1 | |
Mutagenesisi | 349 | I → E: Dimeric form; when associated with R-344. Does not form the high-order complex with SHANK1; when associated with R-344. Does not change its interaction with STX12; when associated with R-344. Reduces localization to the spine; when associated with R-344. Affects dendritic spine structure; when associated with R-344. Decreases synaptic transmission; when associated with R-344. Does not affect glutamate receptors ratio; when associated with R-344. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000191007 | 2 – 366 | Homer protein homolog 1Add BLAST | 365 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylglycineBy similarity | 1 | |
Modified residuei | 318 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q9Z214 |
PRIDEi | Q9Z214 |
PTM databases
iPTMneti | Q9Z214 |
PhosphoSitePlusi | Q9Z214 |
SwissPalmi | Q9Z214 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSRNOG00000047014, Expressed in frontal cortex and 21 other tissues |
ExpressionAtlasi | Q9Z214, baseline and differential |
Genevisiblei | Q9Z214, RN |
Interactioni
Subunit structurei
Tetramer; this tetrameric structure is critical for forming the high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (PubMed:19345194).
Interacts with GRM1, GRM5, ITPR1, DYN3, RYR1, RYR2 and SHANK3 (PubMed:10433269, PubMed:10798399, PubMed:12810060, PubMed:12887973, PubMed:9069287, PubMed:9808458, PubMed:9808459, PubMed:9727012).
Interacts with IFT57 and OPHN1 (PubMed:15034583). Isoform 1 and isoform 2 encode coiled-coil structures that mediate homo- and heteromultimerization.
Interacts with SHANK1; forms high-order polymerized complex with a mesh-like network structure, at least composed of SHANK1, HOMER1 and DLGAP1; the complex formation is SHANK1 multimerization dependent (PubMed:10433269, PubMed:19345194).
Interacts with NFATC4 (By similarity).
Interacts with DAGLA (via PPXXF motif); this interaction is required for the cell membrane localization of DAGLA (PubMed:17584991).
By similarity12 PublicationsBinary interactionsi
Hide detailsQ9Z214
With | #Exp. | IntAct |
---|---|---|
Grm5 [Q3UVX5] from Mus musculus. | 2 | EBI-2338940,EBI-8795045 |
Isoform 1 [Q9Z214-1]
With | #Exp. | IntAct |
---|---|---|
Agap2 [Q8CGU4] | 4 | EBI-4410552,EBI-4409108 |
Grm5 [Q3UVX5] from Mus musculus. | 3 | EBI-4410552,EBI-8795045 |
Homer1 - isoform 2 [Q9Z214-2]
With | #Exp. | IntAct |
---|---|---|
itself | 4 | EBI-2338999,EBI-2338999 |
Shank1 [Q9WV48] | 4 | EBI-2338999,EBI-80909 |
Isoform 3 [Q9Z214-3]
With | #Exp. | IntAct |
---|---|---|
Grm5 [Q3UVX5] from Mus musculus. | 2 | EBI-2339003,EBI-8795045 |
GO - Molecular functioni
- G protein-coupled glutamate receptor binding Source: UniProtKB
- identical protein binding Source: IntAct
- ion channel binding Source: RGD
- scaffold protein binding Source: BHF-UCL
- signaling receptor binding Source: RGD
- type 5 metabotropic glutamate receptor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248180, 16 interactors |
ELMi | Q9Z214 |
IntActi | Q9Z214, 10 interactors |
MINTi | Q9Z214 |
STRINGi | 10116.ENSRNOP00000065989 |
Structurei
Secondary structure
3D structure databases
BMRBi | Q9Z214 |
SMRi | Q9Z214 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9Z214 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 110 | WH1PROSITE-ProRule annotationAdd BLAST | 110 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 302 – 366 | Required for tetramerization1 PublicationAdd BLAST | 65 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 193 – 364 | Sequence analysisAdd BLAST | 172 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QR3K, Eukaryota |
GeneTreei | ENSGT00940000156354 |
HOGENOMi | CLU_033940_0_0_1 |
InParanoidi | Q9Z214 |
OrthoDBi | 1251658at2759 |
PhylomeDBi | Q9Z214 |
Family and domain databases
CDDi | cd01206, EVH1_Homer_Vesl, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR044100, Homer_EVH1 IPR011993, PH-like_dom_sf IPR000697, WH1/EVH1_dom |
Pfami | View protein in Pfam PF00568, WH1, 1 hit |
SMARTi | View protein in SMART SM00461, WH1, 1 hit |
PROSITEi | View protein in PROSITE PS50229, WH1, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGEQPIFSTR AHVFQIDPNT KKNWVPTSKH AVTVSYFYDS TRNVYRIISL
60 70 80 90 100
DGSKAIINST ITPNMTFTKT SQKFGQWADS RANTVYGLGF SSEHHLSKFA
110 120 130 140 150
EKFQEFKEAA RLAKEKSQEK MELTSTPSQE SAGGDLQSPL TPESINGTDD
160 170 180 190 200
ERTPDVTQNS EPRAEPAQNA LPFSHSAGDR TQGLSHASSA ISKHWEAELA
210 220 230 240 250
TLKGNNAKLT AALLESTANV KQWKQQLAAY QEEAERLHKR VTELECVSSQ
260 270 280 290 300
ANAVHSHKTE LSQTVQELEE TLKVKEEEIE RLKQEIDNAR ELQEQRDSLT
310 320 330 340 350
QKLQEVEIRN KDLEGQLSEL EQRLEKSQSE QDAFRSNLKT LLEILDGKIF
360
ELTELRDNLA KLLECS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0H2UI35 | A0A0H2UI35_RAT | Homer protein homolog 1 | Homer1 | 366 | Annotation score: | ||
A0A0G2JYD5 | A0A0G2JYD5_RAT | Homer protein homolog 1 | Homer1 | 190 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 268 | L → R in BAA32477 (PubMed:9727012).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009066 | 176 – 187 | Missing in isoform 2. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_009067 | 176 – 186 | SAGDRTQGLSH → RYTFNSAIMIK in isoform 3. 5 PublicationsAdd BLAST | 11 | |
Alternative sequenceiVSP_009068 | 187 – 366 | Missing in isoform 3. 5 PublicationsAdd BLAST | 180 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U92079 mRNA Translation: AAC53113.1 Different initiation. AB003726 mRNA Translation: BAA21671.1 AF093267 mRNA Translation: AAC71031.1 AF093268 mRNA Translation: AAC71032.1 AB017140 mRNA Translation: BAA34311.1 AB007688 mRNA Translation: BAA32477.1 AJ276327 mRNA Translation: CAB77249.1 AJ276328 mRNA Translation: CAB77250.1 |
RefSeqi | NP_113895.1, NM_031707.1 [Q9Z214-1] XP_006231837.1, XM_006231775.3 [Q9Z214-2] |
Genome annotation databases
Ensembli | ENSRNOT00000073871; ENSRNOP00000066634; ENSRNOG00000047014 [Q9Z214-2] |
GeneIDi | 29546 |
KEGGi | rno:29546 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U92079 mRNA Translation: AAC53113.1 Different initiation. AB003726 mRNA Translation: BAA21671.1 AF093267 mRNA Translation: AAC71031.1 AF093268 mRNA Translation: AAC71032.1 AB017140 mRNA Translation: BAA34311.1 AB007688 mRNA Translation: BAA32477.1 AJ276327 mRNA Translation: CAB77249.1 AJ276328 mRNA Translation: CAB77250.1 |
RefSeqi | NP_113895.1, NM_031707.1 [Q9Z214-1] XP_006231837.1, XM_006231775.3 [Q9Z214-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DDV | X-ray | 1.90 | A | 1-111 | [»] | |
1DDW | X-ray | 1.70 | A | 1-120 | [»] | |
1I2H | X-ray | 1.80 | A | 1-163 | [»] | |
3CVE | X-ray | 1.75 | A/B/C/D | 302-366 | [»] | |
BMRBi | Q9Z214 | |||||
SMRi | Q9Z214 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 248180, 16 interactors |
ELMi | Q9Z214 |
IntActi | Q9Z214, 10 interactors |
MINTi | Q9Z214 |
STRINGi | 10116.ENSRNOP00000065989 |
PTM databases
iPTMneti | Q9Z214 |
PhosphoSitePlusi | Q9Z214 |
SwissPalmi | Q9Z214 |
Proteomic databases
PaxDbi | Q9Z214 |
PRIDEi | Q9Z214 |
Protocols and materials databases
ABCDi | Q9Z214, 4 sequenced antibodies |
Genome annotation databases
Ensembli | ENSRNOT00000073871; ENSRNOP00000066634; ENSRNOG00000047014 [Q9Z214-2] |
GeneIDi | 29546 |
KEGGi | rno:29546 |
Organism-specific databases
CTDi | 9456 |
RGDi | 628725, Homer1 |
Phylogenomic databases
eggNOGi | ENOG502QR3K, Eukaryota |
GeneTreei | ENSGT00940000156354 |
HOGENOMi | CLU_033940_0_0_1 |
InParanoidi | Q9Z214 |
OrthoDBi | 1251658at2759 |
PhylomeDBi | Q9Z214 |
Enzyme and pathway databases
Reactomei | R-RNO-6794361, Neurexins and neuroligins |
Miscellaneous databases
EvolutionaryTracei | Q9Z214 |
PROi | PR:Q9Z214 |
Gene expression databases
Bgeei | ENSRNOG00000047014, Expressed in frontal cortex and 21 other tissues |
ExpressionAtlasi | Q9Z214, baseline and differential |
Genevisiblei | Q9Z214, RN |
Family and domain databases
CDDi | cd01206, EVH1_Homer_Vesl, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR044100, Homer_EVH1 IPR011993, PH-like_dom_sf IPR000697, WH1/EVH1_dom |
Pfami | View protein in Pfam PF00568, WH1, 1 hit |
SMARTi | View protein in SMART SM00461, WH1, 1 hit |
PROSITEi | View protein in PROSITE PS50229, WH1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HOME1_RAT | |
Accessioni | Q9Z214Primary (citable) accession number: Q9Z214 Secondary accession number(s): O08567 Q9QWN5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2003 |
Last sequence update: | November 28, 2003 | |
Last modified: | April 7, 2021 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families