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Protein

DNA-binding protein RFXANK

Gene

Rfxank

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters (By similarity). May also potentiate the activation of RAF1 (PubMed:10329666).By similarity1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • histone deacetylase binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein RFXANK
Alternative name(s):
Ankyrin repeat-containing adapter protein Tvl-1
Regulatory factor X subunit B
Short name:
RFX-B
Regulatory factor X-associated ankyrin-containing protein
Gene namesi
Name:Rfxank
Synonyms:Rfxb, Tvl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1333865 Rfxank

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670501 – 269DNA-binding protein RFXANKAdd BLAST269

Post-translational modificationi

Phosphorylated by RAF1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9Z205
PRIDEiQ9Z205

PTM databases

PhosphoSitePlusiQ9Z205

Expressioni

Tissue specificityi

Expressed primarily in thymus, lung and testis.

Gene expression databases

BgeeiENSMUSG00000036120 Expressed in 279 organ(s), highest expression level in spinal cord
ExpressionAtlasiQ9Z205 baseline and differential
GenevisibleiQ9Z205 MM

Interactioni

Subunit structurei

Forms homodimers (PubMed:10329666). The RFX heterotetrameric complex consists of 2 molecules of RFX5 and one each of RFXAP and RFX-B/RFXANK; with each subunit representing a separate complementation group. Interacts (via ankyrin repeats) with RFX5 (via PxLPxI/L motif); the interaction is direct. RFX forms cooperative DNA binding complexes with X2BP and CBF/NF-Y. RFX associates with CIITA to form an active transcriptional complex (By similarity). Interacts with RAF1 (PubMed:10329666). Interacts with RFX7 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202875, 7 interactors
STRINGi10090.ENSMUSP00000075140

Structurei

3D structure databases

ProteinModelPortaliQ9Z205
SMRiQ9Z205
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati88 – 127ANK 1Add BLAST40
Repeati132 – 161ANK 2Add BLAST30
Repeati165 – 194ANK 3Add BLAST30
Repeati198 – 227ANK 4Add BLAST30
Repeati231 – 260ANK 5Add BLAST30

Domaini

Interacts with RAF-1 via its C-terminal ankyrin repeat domain. The same domain also mediates its homodimerization (PubMed:10329666). The third ankyrin repeat is required for association with the two other RFX subunits; RFX5 and RFXAP. The three central ANK repeats mediate binding to the PxLPxI/L motif of RFX5 (By similarity).By similarity1 Publication

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0502 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00930000150856
HOGENOMiHOG000294108
HOVERGENiHBG017718
InParanoidiQ9Z205
KOiK08062
OMAiGADSGHM
OrthoDBiEOG091G0BD5
PhylomeDBiQ9Z205
TreeFamiTF333112

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR017362 DNA-bd_RFXANK
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF13606 Ank_3, 1 hit
PIRSFiPIRSF038034 DNA-binding_RFXANK, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 4 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 3 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform Long (identifier: Q9Z205-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEPTQVAENL VPNQQPPVPD LEDPEDTRDE SPENSDTVVL SLFPCTPDAV
60 70 80 90 100
NPEADASASS LQGSFLKHST TLTNRQRGNE VSALPATLDS LSIHQLAAQG
110 120 130 140 150
ELSQLKDHLR KGACPACTCL SGNNLINKPD ERGFTPLIWA SAFGEIETVR
160 170 180 190 200
FLLDWGADPH ILAKERESAL SLASMGGYTD IVRLLLDRDV DINIYDWNGG
210 220 230 240 250
TPLLYAVRGN HVKCVEALLA RGADLTTEAD SGYTPMDLAV ALGYRKVQQV
260
MESHILRLFQ STLGPVDPE
Length:269
Mass (Da):29,232
Last modified:May 1, 1999 - v1
Checksum:i5713F335DC669E87
GO
Isoform Short (identifier: Q9Z205-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     112-121: Missing.

Show »
Length:259
Mass (Da):28,325
Checksum:iFC0168DC986D95C9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000285112 – 121Missing in isoform Short. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF094761 mRNA Translation: AAC69884.1
AF123704 mRNA Translation: AAD24798.1
BC010971 mRNA Translation: AAH10971.1
CCDSiCCDS22360.1 [Q9Z205-1]
CCDS85552.1 [Q9Z205-2]
RefSeqiNP_001020760.1, NM_001025589.1 [Q9Z205-2]
NP_035396.1, NM_011266.2 [Q9Z205-1]
UniGeneiMm.161167

Genome annotation databases

EnsembliENSMUST00000075724; ENSMUSP00000075140; ENSMUSG00000036120 [Q9Z205-2]
ENSMUST00000212320; ENSMUSP00000148739; ENSMUSG00000036120 [Q9Z205-1]
GeneIDi19727
KEGGimmu:19727
UCSCiuc009lyt.1 mouse [Q9Z205-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF094761 mRNA Translation: AAC69884.1
AF123704 mRNA Translation: AAD24798.1
BC010971 mRNA Translation: AAH10971.1
CCDSiCCDS22360.1 [Q9Z205-1]
CCDS85552.1 [Q9Z205-2]
RefSeqiNP_001020760.1, NM_001025589.1 [Q9Z205-2]
NP_035396.1, NM_011266.2 [Q9Z205-1]
UniGeneiMm.161167

3D structure databases

ProteinModelPortaliQ9Z205
SMRiQ9Z205
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202875, 7 interactors
STRINGi10090.ENSMUSP00000075140

PTM databases

PhosphoSitePlusiQ9Z205

Proteomic databases

PaxDbiQ9Z205
PRIDEiQ9Z205

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075724; ENSMUSP00000075140; ENSMUSG00000036120 [Q9Z205-2]
ENSMUST00000212320; ENSMUSP00000148739; ENSMUSG00000036120 [Q9Z205-1]
GeneIDi19727
KEGGimmu:19727
UCSCiuc009lyt.1 mouse [Q9Z205-1]

Organism-specific databases

CTDi8625
MGIiMGI:1333865 Rfxank

Phylogenomic databases

eggNOGiKOG0502 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00930000150856
HOGENOMiHOG000294108
HOVERGENiHBG017718
InParanoidiQ9Z205
KOiK08062
OMAiGADSGHM
OrthoDBiEOG091G0BD5
PhylomeDBiQ9Z205
TreeFamiTF333112

Miscellaneous databases

PROiPR:Q9Z205
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036120 Expressed in 279 organ(s), highest expression level in spinal cord
ExpressionAtlasiQ9Z205 baseline and differential
GenevisibleiQ9Z205 MM

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR017362 DNA-bd_RFXANK
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF13606 Ank_3, 1 hit
PIRSFiPIRSF038034 DNA-binding_RFXANK, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 4 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiRFXK_MOUSE
AccessioniPrimary (citable) accession number: Q9Z205
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: November 7, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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