UniProtKB - Q9Z200 (SH2B2_RAT)
SH2B adapter protein 2
Sh2b2
Functioni
Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways. Binds to EPOR and suppresses EPO-induced STAT5 activation, possibly through a masking effect on STAT5 docking sites in EPOR. Suppresses PDGF-induced mitogenesis (By similarity).
Involved in stimulation of glucose uptake by insulin. Involved in coupling from immunoreceptor to Ras signaling. Acts as a negative regulator of cytokine signaling in collaboration with CBL. Induces cytoskeletal reorganization and neurite outgrowth in cultured neurons.
By similarity4 PublicationsGO - Molecular functioni
- identical protein binding Source: IntAct
- SH2 domain binding Source: RGD
- signaling adaptor activity Source: RGD
- transmembrane receptor protein tyrosine kinase adaptor activity Source: RGD
GO - Biological processi
- actin cytoskeleton organization Source: RGD
- antigen receptor-mediated signaling pathway Source: RGD
- B-1 B cell homeostasis Source: RGD
- B cell receptor signaling pathway Source: RGD
- brown fat cell differentiation Source: RGD
- cytokine-mediated signaling pathway Source: RGD
- insulin receptor signaling pathway Source: RGD
- intracellular signal transduction Source: RGD
- nervous system development Source: RGD
- regulation of immune response Source: RGD
- regulation of metabolic process Source: RGD
- regulation of Ras protein signal transduction Source: RGD
Enzyme and pathway databases
Reactomei | R-RNO-1433559, Regulation of KIT signaling R-RNO-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | Recommended name: SH2B adapter protein 2Alternative name(s): Adapter protein with pleckstrin homology and Src homology 2 domains SH2 and PH domain-containing adapter protein APS |
Gene namesi | Name:Sh2b2 Synonyms:Aps |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 69284, Sh2b2 |
Subcellular locationi
Cytoskeleton
- actin filament Source: RGD
- stress fiber Source: RGD
Cytosol
- cytosol Source: Ensembl
Plasma Membrane
- plasma membrane Source: RGD
Other locations
Keywords - Cellular componenti
Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 437 | R → A: Abolishes interaction with phosphorylated INSR. 1 Publication | 1 | |
Mutagenesisi | 455 | K → A: Reduces interaction with phosphorylated INSR. 1 Publication | 1 | |
Mutagenesisi | 457 | K → N: Abolishes interaction with phosphorylated INSR. 1 Publication | 1 | |
Mutagenesisi | 618 | Y → F: Abolishes phosphorylation in response to insulin. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064649 | 1 – 621 | SH2B adapter protein 2Add BLAST | 621 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 47 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 130 | PhosphoserineBy similarity | 1 | |
Modified residuei | 303 | PhosphoserineBy similarity | 1 | |
Modified residuei | 597 | PhosphoserineBy similarity | 1 | |
Modified residuei | 618 | Phosphotyrosine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9Z200 |
PTM databases
iPTMneti | Q9Z200 |
PhosphoSitePlusi | Q9Z200 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000001425, Expressed in spleen and 22 other tissues |
Genevisiblei | Q9Z200, RN |
Interactioni
Subunit structurei
Homodimer.
Interacts with KIT/c-KIT, SHC1, EPOR, PDGFR, VAV1 and VAV3.
Interacts (via N-terminal region) with SHC1.
Interacts (via the phosphorylated C-terminus) with GRB2.
Interacts (via its SH2 domain) with EPOR, INSR and KIT.
Interacts with GRB2 after B-cell antigen receptor stimulation.
Interacts (via PH domain) with VAV3 (By similarity).
Interacts with NTRK1, NTRK2 and NTRK3 (phosphorylated); after stimulation of the receptor by its extracellular ligand and subsequent autophosphorylation of the receptor. Binds INSR, GRB2, ASB6 and CAP. Insulin stimulation leads to dissociation of CAP. Binds CBS only when SH2B2/APS has become phosphorylated. INSR binding does not depend on the phosphorylation of SH2B2/APS.
By similarity6 PublicationsBinary interactionsi
Q9Z200
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-8562298,EBI-8562298 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- SH2 domain binding Source: RGD
- transmembrane receptor protein tyrosine kinase adaptor activity Source: RGD
Protein-protein interaction databases
BioGRIDi | 250306, 1 interactor |
IntActi | Q9Z200, 2 interactors |
MINTi | Q9Z200 |
STRINGi | 10116.ENSRNOP00000001935 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9Z200 |
BMRBi | Q9Z200 |
SMRi | Q9Z200 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9Z200 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 186 – 299 | PHPROSITE-ProRule annotationAdd BLAST | 114 | |
Domaini | 409 – 507 | SH2PROSITE-ProRule annotationAdd BLAST | 99 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 144 – 165 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 507 – 528 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 548 – 609 | DisorderedSequence analysisAdd BLAST | 62 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 548 – 572 | Polar residuesSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Keywords - Domaini
SH2 domainPhylogenomic databases
eggNOGi | ENOG502QT43, Eukaryota |
GeneTreei | ENSGT00950000183191 |
HOGENOMi | CLU_014885_5_0_1 |
InParanoidi | Q9Z200 |
OMAi | KMEGERF |
OrthoDBi | 556279at2759 |
PhylomeDBi | Q9Z200 |
Family and domain databases
CDDi | cd10411, SH2_SH2B2, 1 hit |
Gene3Di | 2.30.29.30, 1 hit 3.30.505.10, 1 hit |
IDEALi | IID50237 |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR015012, Phe_ZIP IPR036290, Phe_ZIP_sf IPR000980, SH2 IPR036860, SH2_dom_sf IPR030523, SH2B IPR030520, SH2B2 IPR035058, SH2B2_SH2 |
PANTHERi | PTHR10872, PTHR10872, 1 hit PTHR10872:SF4, PTHR10872:SF4, 1 hit |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF08916, Phe_ZIP, 1 hit PF00017, SH2, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00252, SH2, 1 hit |
SUPFAMi | SSF109805, SSF109805, 1 hit SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50001, SH2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MNGATPGSAA APAPVPDWRQ FCELHAQVAA VDFAHKFCRF LRDNPTYDTP
60 70 80 90 100
DAGTSFSRHF AANFLAVFSE EVRRVLGTAA DTMEPEPAVT SVTSALKTAT
110 120 130 140 150
YGHSRSSEDV SAHVATKARV RKGFSLRNMS LCVVDGVRDL WHRRASPEPE
160 170 180 190 200
GGATPKTTEP VSEPRDKWTR RLRLARTLAA KVELVDIQRE GALRFMVADD
210 220 230 240 250
AASGPGGTAQ WQKCRLLLRR AVAGERFRLE FFVPPKASRP KVSIPLSAII
260 270 280 290 300
EVRTTMPLEM PEKDNTFVLK VENGAEYILE TIDSLQKHSW VADIQGCVDP
310 320 330 340 350
GDSEEDTGLS CARGGCLASR VTSCSCELLT EADMPRPPET MTAVGAVVTA
360 370 380 390 400
PHGRARDTVG ESLAHVPLET FLQTLESSGG VSESNNTGDE GAELDPDAEA
410 420 430 440 450
ELELSDYPWF HGTLSRVKAA QLVLAGGPRS HGLFVIRQSE TRPGECVLTF
460 470 480 490 500
NFQGKAKHLR LSLNGHGQCH VQHLWFQSVF DMLRHFHTHP IPLESGGSAD
510 520 530 540 550
ITLRSYVRAQ GPPPDPGPAP NTAAPVPACW TEPAGQHYFS SLATATCPPT
560 570 580 590 600
SPSNGAGASS SSGSSSSATS VPPRPAEGPL SARSRSNSTE HLLEAASGAT
610 620
EEPADATLGR ARAVENQYSF Y
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A8I6GLF9 | A0A8I6GLF9_RAT | SH2B adapter protein 2 | Sh2b2 | 418 | Annotation score: | ||
A0A8I6GLZ7 | A0A8I6GLZ7_RAT | SH2B adapter protein 2 | Sh2b2 | 561 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF095576 mRNA Translation: AAC64408.1 |
RefSeqi | NP_446121.1, NM_053669.1 XP_008767335.1, XM_008769113.2 XP_017453727.1, XM_017598238.1 XP_017453728.1, XM_017598239.1 |
Genome annotation databases
Ensembli | ENSRNOT00000001935; ENSRNOP00000001935; ENSRNOG00000001425 |
GeneIDi | 114203 |
KEGGi | rno:114203 |
UCSCi | RGD:69284, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF095576 mRNA Translation: AAC64408.1 |
RefSeqi | NP_446121.1, NM_053669.1 XP_008767335.1, XM_008769113.2 XP_017453727.1, XM_017598238.1 XP_017453728.1, XM_017598239.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1RPY | X-ray | 2.30 | A/B | 401-510 | [»] | |
1RQQ | X-ray | 2.60 | C/D | 401-510 | [»] | |
1YVH | X-ray | 2.05 | B | 609-621 | [»] | |
AlphaFoldDBi | Q9Z200 | |||||
BMRBi | Q9Z200 | |||||
SMRi | Q9Z200 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 250306, 1 interactor |
IntActi | Q9Z200, 2 interactors |
MINTi | Q9Z200 |
STRINGi | 10116.ENSRNOP00000001935 |
PTM databases
iPTMneti | Q9Z200 |
PhosphoSitePlusi | Q9Z200 |
Proteomic databases
PaxDbi | Q9Z200 |
Genome annotation databases
Ensembli | ENSRNOT00000001935; ENSRNOP00000001935; ENSRNOG00000001425 |
GeneIDi | 114203 |
KEGGi | rno:114203 |
UCSCi | RGD:69284, rat |
Organism-specific databases
CTDi | 10603 |
RGDi | 69284, Sh2b2 |
Phylogenomic databases
eggNOGi | ENOG502QT43, Eukaryota |
GeneTreei | ENSGT00950000183191 |
HOGENOMi | CLU_014885_5_0_1 |
InParanoidi | Q9Z200 |
OMAi | KMEGERF |
OrthoDBi | 556279at2759 |
PhylomeDBi | Q9Z200 |
Enzyme and pathway databases
Reactomei | R-RNO-1433559, Regulation of KIT signaling R-RNO-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
EvolutionaryTracei | Q9Z200 |
PROi | PR:Q9Z200 |
Gene expression databases
Bgeei | ENSRNOG00000001425, Expressed in spleen and 22 other tissues |
Genevisiblei | Q9Z200, RN |
Family and domain databases
CDDi | cd10411, SH2_SH2B2, 1 hit |
Gene3Di | 2.30.29.30, 1 hit 3.30.505.10, 1 hit |
IDEALi | IID50237 |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR015012, Phe_ZIP IPR036290, Phe_ZIP_sf IPR000980, SH2 IPR036860, SH2_dom_sf IPR030523, SH2B IPR030520, SH2B2 IPR035058, SH2B2_SH2 |
PANTHERi | PTHR10872, PTHR10872, 1 hit PTHR10872:SF4, PTHR10872:SF4, 1 hit |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF08916, Phe_ZIP, 1 hit PF00017, SH2, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00252, SH2, 1 hit |
SUPFAMi | SSF109805, SSF109805, 1 hit SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50001, SH2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SH2B2_RAT | |
Accessioni | Q9Z200Primary (citable) accession number: Q9Z200 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2005 |
Last sequence update: | May 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families