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UniProtKB - Q9Z1Z3 (EPN2_RAT)
Protein
Epsin-2
Gene
Epn2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Plays a role in the formation of clathrin-coated invaginations and endocytosis.
1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 8 | Phosphatidylinositol 4,5-bisphosphateBy similarity | 1 | |
Binding sitei | 11 | Phosphatidylinositol 4,5-bisphosphateBy similarity | 1 | |
Binding sitei | 25 | Phosphatidylinositol 4,5-bisphosphateBy similarity | 1 | |
Binding sitei | 30 | Phosphatidylinositol 4,5-bisphosphateBy similarity | 1 | |
Binding sitei | 63 | Phosphatidylinositol 4,5-bisphosphateBy similarity | 1 | |
Binding sitei | 73 | Phosphatidylinositol 4,5-bisphosphateBy similarity | 1 |
GO - Molecular functioni
- clathrin binding Source: GO_Central
- phospholipid binding Source: GO_Central
GO - Biological processi
- embryonic organ development Source: RGD
- endocytosis Source: GO_Central
- in utero embryonic development Source: RGD
- negative regulation of sprouting angiogenesis Source: RGD
- negative regulation of vascular endothelial growth factor receptor signaling pathway Source: RGD
- Notch signaling pathway Source: RGD
- positive regulation of Notch signaling pathway Source: RGD
- regulation of endocytosis Source: RGD
Keywordsi
Biological process | Endocytosis |
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-RNO-8856825, Cargo recognition for clathrin-mediated endocytosis R-RNO-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Epsin-2Alternative name(s): EPS-15-interacting protein 2 |
Gene namesi | Name:Epn2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619773, Epn2 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: In punctate structures throughout the cell and particularly concentrated in the region of the Golgi complex.By similarity
Endosome
- endosome Source: GO_Central
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- clathrin coat of endocytic vesicle Source: RGD
- clathrin vesicle coat Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074518 | 1 – 583 | Epsin-2Add BLAST | 583 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 170 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 173 | PhosphoserineBy similarity | 1 | |
Modified residuei | 192 | PhosphoserineCombined sources | 1 | |
Modified residuei | 195 | PhosphoserineCombined sources | 1 | |
Modified residuei | 431 | PhosphoserineCombined sources | 1 | |
Modified residuei | 453 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 514 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Ubiquitinated.1 Publication
Keywords - PTMi
Methylation, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q9Z1Z3 |
PRIDEi | Q9Z1Z3 |
PTM databases
iPTMneti | Q9Z1Z3 |
Expressioni
Tissue specificityi
Highly expressed in brain. Detected at lower levels in lung, liver, muscle and testis.1 Publication
Interactioni
Subunit structurei
Binds AP-2 and clathrin (By similarity).
Interacts with ITSN1 (By similarity).
Interacts with UBQLN2 (By similarity). Binds EPS15.
By similarity1 PublicationGO - Molecular functioni
- clathrin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 248836, 2 interactors |
STRINGi | 10116.ENSRNOP00000039386 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 144 | ENTHPROSITE-ProRule annotationAdd BLAST | 133 | |
Domaini | 218 – 237 | UIM 1PROSITE-ProRule annotationAdd BLAST | 20 | |
Domaini | 243 – 262 | UIM 2PROSITE-ProRule annotationAdd BLAST | 20 | |
Repeati | 301 – 303 | 1 | 3 | |
Repeati | 313 – 315 | 2 | 3 | |
Repeati | 326 – 328 | 3 | 3 | |
Repeati | 340 – 342 | 4 | 3 | |
Repeati | 358 – 360 | 5 | 3 | |
Repeati | 375 – 377 | 6 | 3 | |
Repeati | 482 – 484 | 1 | 3 | |
Repeati | 496 – 498 | 2 | 3 | |
Repeati | 579 – 581 | 3 | 3 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 165 – 217 | DisorderedSequence analysisAdd BLAST | 53 | |
Regioni | 293 – 384 | DisorderedSequence analysisAdd BLAST | 92 | |
Regioni | 301 – 377 | 6 X 3 AA repeats of [DE]-P-WAdd BLAST | 77 | |
Regioni | 411 – 457 | DisorderedSequence analysisAdd BLAST | 47 | |
Regioni | 482 – 581 | 3 X 3 AA repeats of N-P-FAdd BLAST | 100 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 165 – 185 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 194 – 217 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 297 – 319 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 344 – 370 | Polar residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 422 – 455 | Polar residuesSequence analysisAdd BLAST | 34 |
Domaini
The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP-2 and clathrin binding.
Sequence similaritiesi
Belongs to the epsin family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
InParanoidi | Q9Z1Z3 |
Family and domain databases
Gene3Di | 1.25.40.90, 1 hit |
InterProi | View protein in InterPro IPR013809, ENTH IPR008942, ENTH_VHS IPR027319, Epsin-2_metazoa IPR003903, UIM_dom |
PANTHERi | PTHR12276:SF50, PTHR12276:SF50, 1 hit |
Pfami | View protein in Pfam PF01417, ENTH, 1 hit |
SMARTi | View protein in SMART SM00273, ENTH, 1 hit SM00726, UIM, 2 hits |
SUPFAMi | SSF48464, SSF48464, 1 hit |
PROSITEi | View protein in PROSITE PS50942, ENTH, 1 hit PS50330, UIM, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q9Z1Z3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTSSIRRQM KNIVNSYSEA EIKVREATSN DPWGPSSSLM TEIADLTYNV
60 70 80 90 100
VRFSEIMSMV WKRLNDHGKN WRHVYKALTL LDYLIKTGSE RVAQQCRENI
110 120 130 140 150
FAIQTLKDFQ YIDRDGKDQG INVREKSKQL VALLKDEERL KVERVQALKT
160 170 180 190 200
KERMAQVATG VGSNQITFGR GSSQPNLSIS HSEQEYGKAG GSPASYHGST
210 220 230 240 250
SPRVSSELEQ ARPQTSGEEE LQLQLALAMS REVAEQEERL RRGDDLRLQM
260 270 280 290 300
ALEESRRDTV KVPKKKEVKA CCKPGSHSQQ TTLLDLMDAL PSSGPVAQKT
310 320 330 340 350
EPWSTGTPAN QTNPWGGTVA PANISDPWPS FGTKPAASVD PWGVPTTASI
360 370 380 390 400
QSVPKNSDPW AASQQPASDA GKTADAWGAA KPSPASGSFE LFSNFNGTVK
410 420 430 440 450
DDFSEFDNLR TSKKPAESGA SVPPQDSRTT SPDLFESQSL TSASSKPSSA
460 470 480 490 500
RKTPESFLGP NAALVNLDSL VTKPAPPAQS LNPFLAPGAA APAPVNPFQV
510 520 530 540 550
NQPQPLTLNQ LRGSPVLGSS ASFGSGPGVE TVAPMPSVAP HSALGATGSS
560 570 580
LTPLGPTAMN MVGSMGIPPS AAQPAGTTNP FLL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ505I9 | Q505I9_RAT | Epsin-2 | Epn2 | 640 | Annotation score: | ||
F1LQ45 | F1LQ45_RAT | Epsin-2 | Epn2 | 583 | Annotation score: | ||
A0A0G2JXY9 | A0A0G2JXY9_RAT | Epsin-2 | Epn2 | 604 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF096269 mRNA Translation: AAC79495.1 |
Genome annotation databases
UCSCi | RGD:619773, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF096269 mRNA Translation: AAC79495.1 |
3D structure databases
SMRi | Q9Z1Z3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248836, 2 interactors |
STRINGi | 10116.ENSRNOP00000039386 |
PTM databases
iPTMneti | Q9Z1Z3 |
Proteomic databases
jPOSTi | Q9Z1Z3 |
PRIDEi | Q9Z1Z3 |
Genome annotation databases
UCSCi | RGD:619773, rat |
Organism-specific databases
RGDi | 619773, Epn2 |
Phylogenomic databases
InParanoidi | Q9Z1Z3 |
Enzyme and pathway databases
Reactomei | R-RNO-8856825, Cargo recognition for clathrin-mediated endocytosis R-RNO-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
PROi | PR:Q9Z1Z3 |
Family and domain databases
Gene3Di | 1.25.40.90, 1 hit |
InterProi | View protein in InterPro IPR013809, ENTH IPR008942, ENTH_VHS IPR027319, Epsin-2_metazoa IPR003903, UIM_dom |
PANTHERi | PTHR12276:SF50, PTHR12276:SF50, 1 hit |
Pfami | View protein in Pfam PF01417, ENTH, 1 hit |
SMARTi | View protein in SMART SM00273, ENTH, 1 hit SM00726, UIM, 2 hits |
SUPFAMi | SSF48464, SSF48464, 1 hit |
PROSITEi | View protein in PROSITE PS50942, ENTH, 1 hit PS50330, UIM, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | EPN2_RAT | |
Accessioni | Q9Z1Z3Primary (citable) accession number: Q9Z1Z3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 16, 2004 |
Last sequence update: | May 1, 1999 | |
Last modified: | February 23, 2022 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families