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UniProtKB - Q9Z1Y9 (IOD2_MOUSE)
Protein
Type II iodothyronine deiodinase
Gene
Dio2
Organism
Mus musculus (Mouse)
Status
Functioni
Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.
By similarityCatalytic activityi
- EC:1.21.99.4PROSITE-ProRule annotation
Kineticsi
- KM=4.4 nM for T41 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 130 | 1 |
GO - Molecular functioni
- thyroxine 5'-deiodinase activity Source: UniProtKB
- thyroxine 5-deiodinase activity Source: MGI
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- brown fat cell differentiation Source: MGI
- cellular lipid metabolic process Source: MGI
- hormone biosynthetic process Source: UniProtKB-KW
- positive regulation of cold-induced thermogenesis Source: YuBioLab
- response to cold Source: MGI
- response to lipopolysaccharide Source: Ensembl
- thyroid hormone catabolic process Source: MGI
- thyroid hormone generation Source: MGI
- thyroid hormone metabolic process Source: MGI
- thyroid-stimulating hormone secretion Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Thyroid hormones biosynthesis |
Enzyme and pathway databases
BRENDAi | 1.21.99.4, 3474 |
Reactomei | R-MMU-350864, Regulation of thyroid hormone activity |
SABIO-RKi | Q9Z1Y9 |
Names & Taxonomyi
Protein namesi | Recommended name: Type II iodothyronine deiodinase (EC:1.21.99.4)Alternative name(s): 5DII DIOII Type 2 DI Type-II 5'-deiodinase |
Gene namesi | Name:Dio2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1338833, Dio2 |
VEuPathDBi | HostDB:ENSMUSG00000007682 |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass membrane protein Curated
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 10 – 34 | HelicalSequence analysisAdd BLAST | 25 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154318 | 1 – 266 | Type II iodothyronine deiodinaseAdd BLAST | 266 |
Post-translational modificationi
Ubiquitinated by MARCHF6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33 (By similarity).By similarity
Keywords - PTMi
Ubl conjugationProteomic databases
PaxDbi | Q9Z1Y9 |
PRIDEi | Q9Z1Y9 |
ProteomicsDBi | 269074 |
Expressioni
Tissue specificityi
Expressed in mammary gland and in brain.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000007682, Expressed in uterus and 217 other tissues |
Genevisiblei | Q9Z1Y9, MM |
Interactioni
Subunit structurei
Interacts with USP20 and USP33.
Interacts with MARCHF6 (By similarity).
By similarityGO - Molecular functioni
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000081013 |
Miscellaneous databases
RNActi | Q9Z1Y9, protein |
Structurei
Family & Domainsi
Sequence similaritiesi
Belongs to the iodothyronine deiodinase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QS2F, Eukaryota |
GeneTreei | ENSGT00940000154482 |
HOGENOMi | CLU_089345_1_0_1 |
InParanoidi | Q9Z1Y9 |
OMAi | PRWSSTI |
OrthoDBi | 977776at2759 |
PhylomeDBi | Q9Z1Y9 |
TreeFami | TF329721 |
Family and domain databases
InterProi | View protein in InterPro IPR000643, Iodothyronine_deiodinase IPR008261, Iodothyronine_deiodinase_AS IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR11781, PTHR11781, 1 hit |
Pfami | View protein in Pfam PF00837, T4_deiodinase, 1 hit |
PIRSFi | PIRSF001330, IOD, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS01205, T4_DEIODINASE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9Z1Y9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGLLSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVALL LSRSKSTRGE
60 70 80 90 100
WRRMLTSEGL RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPESGNNYAS
110 120 130 140 150
EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF RQLVEEFSSV
160 170 180 190 200
ADFLLVYIDE AHPSDGWAVP GDSSLSFEVK KHRNQEDRCA AAHQLLERFS
210 220 230 240 250
LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE
260
VRSWLEKNFS KRUILD
Non-standard residue
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-standard residuei | 130 | Selenocysteine | 1 | |
Non-standard residuei | 263 | Selenocysteine | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF096875 mRNA Translation: AAD11422.1 AF177196 mRNA Translation: AAD53113.1 AF093137 mRNA Translation: AAF00069.2 BC125383 mRNA Translation: AAI25384.1 BC125385 mRNA Translation: AAI25386.1 |
RefSeqi | NP_034180.1, NM_010050.3 |
Genome annotation databases
Ensembli | ENSMUST00000082432; ENSMUSP00000081013; ENSMUSG00000007682 |
GeneIDi | 13371 |
KEGGi | mmu:13371 |
UCSCi | uc007okf.1, mouse |
Keywords - Coding sequence diversityi
SelenocysteineSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF096875 mRNA Translation: AAD11422.1 AF177196 mRNA Translation: AAD53113.1 AF093137 mRNA Translation: AAF00069.2 BC125383 mRNA Translation: AAI25384.1 BC125385 mRNA Translation: AAI25386.1 |
RefSeqi | NP_034180.1, NM_010050.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000081013 |
Proteomic databases
PaxDbi | Q9Z1Y9 |
PRIDEi | Q9Z1Y9 |
ProteomicsDBi | 269074 |
Protocols and materials databases
Antibodypediai | 47379, 212 antibodies from 32 providers |
DNASUi | 13371 |
Genome annotation databases
Ensembli | ENSMUST00000082432; ENSMUSP00000081013; ENSMUSG00000007682 |
GeneIDi | 13371 |
KEGGi | mmu:13371 |
UCSCi | uc007okf.1, mouse |
Organism-specific databases
CTDi | 1734 |
MGIi | MGI:1338833, Dio2 |
VEuPathDBi | HostDB:ENSMUSG00000007682 |
Phylogenomic databases
eggNOGi | ENOG502QS2F, Eukaryota |
GeneTreei | ENSGT00940000154482 |
HOGENOMi | CLU_089345_1_0_1 |
InParanoidi | Q9Z1Y9 |
OMAi | PRWSSTI |
OrthoDBi | 977776at2759 |
PhylomeDBi | Q9Z1Y9 |
TreeFami | TF329721 |
Enzyme and pathway databases
BRENDAi | 1.21.99.4, 3474 |
Reactomei | R-MMU-350864, Regulation of thyroid hormone activity |
SABIO-RKi | Q9Z1Y9 |
Miscellaneous databases
BioGRID-ORCSi | 13371, 0 hits in 64 CRISPR screens |
ChiTaRSi | Dio2, mouse |
PROi | PR:Q9Z1Y9 |
RNActi | Q9Z1Y9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000007682, Expressed in uterus and 217 other tissues |
Genevisiblei | Q9Z1Y9, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000643, Iodothyronine_deiodinase IPR008261, Iodothyronine_deiodinase_AS IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR11781, PTHR11781, 1 hit |
Pfami | View protein in Pfam PF00837, T4_deiodinase, 1 hit |
PIRSFi | PIRSF001330, IOD, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS01205, T4_DEIODINASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IOD2_MOUSE | |
Accessioni | Q9Z1Y9Primary (citable) accession number: Q9Z1Y9 Secondary accession number(s): Q05A70, Q9JHH1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | February 26, 2008 | |
Last modified: | February 23, 2022 | |
This is version 157 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families