UniProtKB - Q9Z1X9 (CDC45_MOUSE)
Protein
Cell division control protein 45 homolog
Gene
Cdc45
Organism
Mus musculus (Mouse)
Status
Functioni
Required for initiation of chromosomal DNA replication.
GO - Molecular functioni
- chromatin binding Source: GO_Central
- DNA replication origin binding Source: GO_Central
- single-stranded DNA binding Source: GO_Central
GO - Biological processi
- DNA replication initiation Source: GO_Central
- double-strand break repair via break-induced replication Source: GO_Central
- mitotic DNA replication preinitiation complex assembly Source: GO_Central
- positive regulation of G1/S transition of mitotic cell cycle Source: GO_Central
- regulation of chromatin silencing at telomere Source: GO_Central
Keywordsi
Biological process | Cell cycle, DNA replication |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68962, Activation of the pre-replicative complex |
Names & Taxonomyi
Protein namesi | Recommended name: Cell division control protein 45 homologAlternative name(s): PORC-PI-1 |
Gene namesi | Name:Cdc45 Synonyms:Cdc45l, Cdc45l2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1338073, Cdc45 |
Subcellular locationi
Nucleus
Cytoskeleton
- centrosome Source: MGI
- ciliary basal body Source: MGI
Nucleus
- DNA replication preinitiation complex Source: GO_Central
- nuclear pre-replicative complex Source: GO_Central
- nucleoplasm Source: MGI
- nucleus Source: MGI
- replication fork protection complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000192816 | 1 – 566 | Cell division control protein 45 homologAdd BLAST | 566 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 130 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 144 | PhosphoserineCombined sources | 1 | |
Modified residuei | 146 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9Z1X9 |
MaxQBi | Q9Z1X9 |
PaxDbi | Q9Z1X9 |
PRIDEi | Q9Z1X9 |
PTM databases
iPTMneti | Q9Z1X9 |
PhosphoSitePlusi | Q9Z1X9 |
Expressioni
Tissue specificityi
Widely expressed.
Gene expression databases
Bgeei | ENSMUSG00000000028, Expressed in cardiac ventricle and 218 other tissues |
ExpressionAtlasi | Q9Z1X9, baseline and differential |
Genevisiblei | Q9Z1X9, MM |
Interactioni
Subunit structurei
Associated with ORC2.
Interacts with HELB (By similarity).
Component of the CMG helicase complex, composed of the MCM2-7 complex, the GINS complex and CDC45 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 198628, 1 interactor |
ComplexPortali | CPX-4541, CMG helicase complex |
IntActi | Q9Z1X9, 2 interactors |
STRINGi | 10090.ENSMUSP00000000028 |
Miscellaneous databases
RNActi | Q9Z1X9, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the CDC45 family.Curated
Phylogenomic databases
eggNOGi | KOG2475, Eukaryota |
GeneTreei | ENSGT00390000009662 |
HOGENOMi | CLU_005871_4_0_1 |
InParanoidi | Q9Z1X9 |
OMAi | PWNLANV |
OrthoDBi | 1309031at2759 |
PhylomeDBi | Q9Z1X9 |
TreeFami | TF101062 |
Family and domain databases
InterProi | View protein in InterPro IPR003874, CDC45 |
PANTHERi | PTHR10507, PTHR10507, 1 hit |
Pfami | View protein in Pfam PF02724, CDC45, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9Z1X9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFVTDFRKEF YETVHNQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV
60 70 80 90 100
PVSGWQELET AYLEHKEQFS YFILINCGAN VDLLDILQPD EDSIFFVCDT
110 120 130 140 150
HRPVNVVNVY NDTQIKLLIK QEDDLEVPAY DDIFRDEAED EDLSDSDGDG
160 170 180 190 200
SEPSEKRTRL EEEIVERNRK RRQRREWEAR RKDILFDYEQ YEYYGTSSAM
210 220 230 240 250
VMFDLAWMMS KDLNDMLWWA IVGLTDQWVH DKITQMKYVT DVGILQRHVS
260 270 280 290 300
RHNHRNEAEE NMLSVDCTRI SFEYDLCLVL YQHWSLHESL YNTSYTAARF
310 320 330 340 350
KLWSVHGQKR LQEFLADMGL PLKQVKQKFQ SMDVSLKGNL REMIEESANK
360 370 380 390 400
FGMKDMRVQT FSIQFGFKHK FLASDVVFAT MSLMESPEKD GSGTDHFIQA
410 420 430 440 450
LDSLSRSNLD KLYLGLELAK KHLQATQQTI ASCLCTNLVT SQGPFLYCSL
460 470 480 490 500
MEGTPDVTLF SKPASLSLLS RHLLKSFVYS TKNRRCKLLP LVMAAPLSVE
510 520 530 540 550
QGTVTVVGIP PETDSSDRKN FFGRAFEKAA ESTSSRTLHN YFDLSVIELK
560
AEDRSKFLDA LVSLLS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8WJ72 | F8WJ72_MOUSE | Cell division control protein 45 ho... | Cdc45 | 520 | Annotation score: | ||
D3Z0L5 | D3Z0L5_MOUSE | Cell division control protein 45 ho... | Cdc45 | 136 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 142 | D → Y in AAC95057 (Ref. 1) Curated | 1 | |
Sequence conflicti | 168 – 177 | NRKRRQRREW → ETGRGGRPGSG in CAA11531 (PubMed:9724329).Curated | 10 | |
Sequence conflicti | 175 | R → K in AAD08999 (PubMed:10051334).Curated | 1 | |
Sequence conflicti | 175 | R → K in AAD09000 (PubMed:10051334).Curated | 1 | |
Sequence conflicti | 175 | R → K in AAD09001 (PubMed:10051334).Curated | 1 | |
Sequence conflicti | 197 | S → T in CAA11531 (PubMed:9724329).Curated | 1 | |
Sequence conflicti | 249 | V → L in CAA11531 (PubMed:9724329).Curated | 1 | |
Sequence conflicti | 337 – 342 | KGNLRE → EGESAR in CAA11531 (PubMed:9724329).Curated | 6 | |
Sequence conflicti | 373 | A → T in CAA11531 (PubMed:9724329).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF098068 mRNA Translation: AAC95057.1 AJ223729 mRNA Translation: CAA11531.1 AF081536 mRNA Translation: AAD08999.1 AF081537 mRNA Translation: AAD09000.1 AF081538 mRNA Translation: AAD09001.1 AF081539 mRNA Translation: AAD09002.1 BC028635 mRNA Translation: AAH28635.1 |
CCDSi | CCDS28027.1 |
RefSeqi | NP_033992.2, NM_009862.2 XP_006521807.1, XM_006521744.2 XP_011244115.1, XM_011245813.2 |
Genome annotation databases
Ensembli | ENSMUST00000000028; ENSMUSP00000000028; ENSMUSG00000000028 |
GeneIDi | 12544 |
KEGGi | mmu:12544 |
UCSCi | uc007yom.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF098068 mRNA Translation: AAC95057.1 AJ223729 mRNA Translation: CAA11531.1 AF081536 mRNA Translation: AAD08999.1 AF081537 mRNA Translation: AAD09000.1 AF081538 mRNA Translation: AAD09001.1 AF081539 mRNA Translation: AAD09002.1 BC028635 mRNA Translation: AAH28635.1 |
CCDSi | CCDS28027.1 |
RefSeqi | NP_033992.2, NM_009862.2 XP_006521807.1, XM_006521744.2 XP_011244115.1, XM_011245813.2 |
3D structure databases
SMRi | Q9Z1X9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198628, 1 interactor |
ComplexPortali | CPX-4541, CMG helicase complex |
IntActi | Q9Z1X9, 2 interactors |
STRINGi | 10090.ENSMUSP00000000028 |
PTM databases
iPTMneti | Q9Z1X9 |
PhosphoSitePlusi | Q9Z1X9 |
Proteomic databases
EPDi | Q9Z1X9 |
MaxQBi | Q9Z1X9 |
PaxDbi | Q9Z1X9 |
PRIDEi | Q9Z1X9 |
Protocols and materials databases
Antibodypediai | 262, 348 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000000028; ENSMUSP00000000028; ENSMUSG00000000028 |
GeneIDi | 12544 |
KEGGi | mmu:12544 |
UCSCi | uc007yom.2, mouse |
Organism-specific databases
CTDi | 8318 |
MGIi | MGI:1338073, Cdc45 |
Phylogenomic databases
eggNOGi | KOG2475, Eukaryota |
GeneTreei | ENSGT00390000009662 |
HOGENOMi | CLU_005871_4_0_1 |
InParanoidi | Q9Z1X9 |
OMAi | PWNLANV |
OrthoDBi | 1309031at2759 |
PhylomeDBi | Q9Z1X9 |
TreeFami | TF101062 |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68962, Activation of the pre-replicative complex |
Miscellaneous databases
BioGRID-ORCSi | 12544, 7 hits in 18 CRISPR screens |
ChiTaRSi | Cdc45, mouse |
PROi | PR:Q9Z1X9 |
RNActi | Q9Z1X9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000000028, Expressed in cardiac ventricle and 218 other tissues |
ExpressionAtlasi | Q9Z1X9, baseline and differential |
Genevisiblei | Q9Z1X9, MM |
Family and domain databases
InterProi | View protein in InterPro IPR003874, CDC45 |
PANTHERi | PTHR10507, PTHR10507, 1 hit |
Pfami | View protein in Pfam PF02724, CDC45, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDC45_MOUSE | |
Accessioni | Q9Z1X9Primary (citable) accession number: Q9Z1X9 Secondary accession number(s): O70547, Q9QUK1, Q9R212 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | December 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 135 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families