UniProtKB - Q9Z1X4 (ILF3_MOUSE)
Interleukin enhancer-binding factor 3
Ilf3
Functioni
GO - Molecular functioni
- DNA binding Source: MGI
- double-stranded RNA binding Source: MGI
- enzyme binding Source: MGI
- mRNA 3'-UTR AU-rich region binding Source: GO_Central
- single-stranded RNA binding Source: GO_Central
GO - Biological processi
- defense response to virus Source: UniProtKB-KW
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of translation Source: UniProtKB
- negative regulation of viral genome replication Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: MGI
- protein methylation Source: MGI
- protein phosphorylation Source: UniProtKB
Keywordsi
Molecular function | DNA-binding, RNA-binding |
Biological process | Antiviral defense, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Interleukin enhancer-binding factor 3 |
Gene namesi | Name:Ilf3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1339973, Ilf3 |
Subcellular locationi
Nucleus
Other locations
- Cytoplasm 1 Publication
Note: Localizes in the cytoplasm in response to viral infection. The unphosphorylated form is retained in the nucleus by ILF2. Phosphorylation at Thr-188 and Thr-315 causes the dissociation of ILF2 from the ILF2-ILF3 complex resulting in a cytoplasmic sequestration of ILF3. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.1 Publication
Mitochondrion
- mitochondrion Source: MGI
Nucleus
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- ribonucleoprotein complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000126071 | 1 – 898 | Interleukin enhancer-binding factor 3Add BLAST | 898 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 67 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 100 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 188 | Phosphothreonine; by PKR1 Publication | 1 | |
Modified residuei | 190 | PhosphoserineCombined sources | 1 | |
Modified residuei | 315 | Phosphothreonine; by PKR1 Publication | 1 | |
Cross-linki | 348 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity | ||
Modified residuei | 382 | PhosphoserineBy similarity | 1 | |
Modified residuei | 384 | PhosphoserineBy similarity | 1 | |
Cross-linki | 396 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 460 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 476 | PhosphoserineBy similarity | 1 | |
Modified residuei | 477 | PhosphoserineBy similarity | 1 | |
Modified residuei | 482 | PhosphoserineCombined sources | 1 | |
Modified residuei | 486 | PhosphoserineCombined sources | 1 | |
Cross-linki | 489 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 592 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 794 | PhosphoserineBy similarity | 1 | |
Modified residuei | 812 | PhosphoserineBy similarity | 1 | |
Modified residuei | 814 | PhosphoserineBy similarity | 1 | |
Modified residuei | 818 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9Z1X4 |
jPOSTi | Q9Z1X4 |
PaxDbi | Q9Z1X4 |
PeptideAtlasi | Q9Z1X4 |
PRIDEi | Q9Z1X4 |
PTM databases
iPTMneti | Q9Z1X4 |
PhosphoSitePlusi | Q9Z1X4 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000032178, Expressed in undifferentiated genital tubercle and 355 other tissues |
ExpressionAtlasi | Q9Z1X4, baseline and differential |
Genevisiblei | Q9Z1X4, MM |
Interactioni
Subunit structurei
Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs.
Interacts with FUS and SMN.
Interacts (via C-terminus) with PRMT1.
Forms a complex with ILF2. Can also bind to PRKDC/XRCC7: this may stabilize the interaction of PRKDC/XRCC7 and the heterodimeric complex of XRCC6/KU70 and XRCC5/KU80.
Forms a heteromeric complex with ZNF346 and ILF3.
Found in a nuclear export complex with XPO5, ILF3, Ran and double-stranded RNA or double-stranded minihelix VA1 RNA.
Found in a nuclear export complex with XPO5, RAN, ILF3, ZNF346 and double-stranded RNA.
Interacts with XPO5 and ZNF346.
Forms a complex with ILF2, YLPM1, KHDRBS1, RBMX, NCOA5 and PPP1CA.
Interacts with AGO1 and AGO2.
Interacts with DHX36; this interaction occurs in a RNA-dependent manner.
Interacts with ELAVL1; this interaction occurs in a RNA-dependent manner (By similarity).
By similarityGO - Molecular functioni
- enzyme binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 200648, 59 interactors |
IntActi | Q9Z1X4, 4 interactors |
MINTi | Q9Z1X4 |
STRINGi | 10090.ENSMUSP00000065770 |
Miscellaneous databases
RNActi | Q9Z1X4, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9Z1X4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 378 | DZFPROSITE-ProRule annotationAdd BLAST | 374 | |
Domaini | 398 – 467 | DRBM 1PROSITE-ProRule annotationAdd BLAST | 70 | |
Domaini | 524 – 590 | DRBM 2PROSITE-ProRule annotationAdd BLAST | 67 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 609 – 898 | Interaction with PRMT1By similarityAdd BLAST | 290 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 371 – 389 | Bipartite nuclear localization signalSequence analysisAdd BLAST | 19 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 385 – 389 | Poly-Lys | 5 | |
Compositional biasi | 634 – 637 | Poly-Pro | 4 | |
Compositional biasi | 640 – 659 | Arg/Gly-richAdd BLAST | 20 | |
Compositional biasi | 701 – 710 | Poly-Gly | 10 | |
Compositional biasi | 796 – 800 | Poly-Gly | 5 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3792, Eukaryota |
GeneTreei | ENSGT00940000156719 |
InParanoidi | Q9Z1X4 |
OMAi | YSHGQGN |
OrthoDBi | 612611at2759 |
PhylomeDBi | Q9Z1X4 |
TreeFami | TF320194 |
Family and domain databases
CDDi | cd00048, DSRM, 2 hits |
DisProti | DP01562 |
Gene3Di | 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR014720, dsRBD_dom IPR006561, DZF_dom IPR033099, ILF3/NF90 IPR043519, NT_sf |
PANTHERi | PTHR45762:SF4, PTHR45762:SF4, 3 hits |
Pfami | View protein in Pfam PF00035, dsrm, 2 hits PF07528, DZF, 1 hit |
SMARTi | View protein in SMART SM00358, DSRM, 2 hits SM00572, DZF, 1 hit |
PROSITEi | View protein in PROSITE PS50137, DS_RBD, 2 hits PS51703, DZF, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRPMRIFVND DRHVMAKHSS VYPTQEELEA VQNMVSHTER ALKAVSDWID
60 70 80 90 100
EQEKGNSELS EAENMDTPPD DESKEGAGEQ KAEHMTRTLR GVMRVGLVAK
110 120 130 140 150
GLLLKGDLDL ELVLLCKEKP TTALLDKVAD NLAIQLTTVT EDKYEILQSV
160 170 180 190 200
DDAAIVIKNT KEPPLSLTIH LTSPVVREEM EKVLAGETLS VNDPPDVLDR
210 220 230 240 250
QKCLAALASL RHAKWFQARA NGLKSCVIVI RVLRDLCTRV PTWGPLRGWP
260 270 280 290 300
LELLCEKSIG TANRPMGAGE ALRRVLECLA SGIVMPDGSG IYDPCEKEAT
310 320 330 340 350
DAIGHLDRQQ REDITQSAQH ALRLAAFGQL HKVLGMDPLP SKMPKKPKNE
360 370 380 390 400
NPVDYTVQIP PSTTYAITPM KRPMEEDGEE KSPSKKKKKI QKKEEKADPP
410 420 430 440 450
QAMNALMRLN QLKPGLQYKL ISQTGPVHAP IFTMSVEVDG SNFEASGPSK
460 470 480 490 500
KTAKLHVAVK VLQDMGLPTG AEGRDSSKGE DSAEESDGKP AIVAPPPVVE
510 520 530 540 550
AVSNPSSVFP SDATTEQGPI LTKHGKNPVM ELNEKRRGLK YELISETGGS
560 570 580 590 600
HDKRFVMEVE VDGQKFQGAG SNKKVAKAYA ALAALEKLFP DTPLALEANK
610 620 630 640 650
KKRTPVPVRG GPKFAAKPHN PGFGMGGPMH NEVPPPPNIR GRGRGGNIRG
660 670 680 690 700
RGRGRGFGGA NHGGGYMNAG AGYGSYGYSS NSATAGYSQF YSNGGHSGNA
710 720 730 740 750
GGGGSGGGGG SSSYSSYYQG DSYNSPVPPK HAGKKPLHGG QQKASYSSGY
760 770 780 790 800
QSHQGQQQPY NQSQYSSYGT PQGKQKGYGH GQGSYSSYSN SYNSPGGGGG
810 820 830 840 850
SDYSYDSKFN YSGSGGRSGG NSYGSSGSSS YNTGSHGGYG TGSGGSSSYQ
860 870 880 890
GKQGGYSSQS NYSSPGSSQS YSGPASSYQS SQGGYSRNTE HSMNYQYR
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ45VK5 | Q45VK5_MOUSE | Interleukin enhancer-binding factor... | Ilf3 | 703 | Annotation score: | ||
A0A1L1STE4 | A0A1L1STE4_MOUSE | Interleukin enhancer-binding factor... | Ilf3 | 908 | Annotation score: | ||
A0A1L1SSU8 | A0A1L1SSU8_MOUSE | Interleukin enhancer-binding factor... | Ilf3 | 168 | Annotation score: | ||
A0A1L1ST48 | A0A1L1ST48_MOUSE | Interleukin enhancer-binding factor... | Ilf3 | 214 | Annotation score: | ||
A0A1L1SU19 | A0A1L1SU19_MOUSE | Interleukin enhancer-binding factor... | Ilf3 | 126 | Annotation score: | ||
A0A1L1SQR7 | A0A1L1SQR7_MOUSE | Interleukin enhancer-binding factor... | Ilf3 | 177 | Annotation score: | ||
A0A1L1SQ69 | A0A1L1SQ69_MOUSE | Interleukin enhancer-binding factor... | Ilf3 | 77 | Annotation score: | ||
A0A1L1SR62 | A0A1L1SR62_MOUSE | Interleukin enhancer-binding factor... | Ilf3 | 74 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 796 | G → C in AAC71052 (PubMed:10337617).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013406 | 1 | M → MALYHHHFITRRRR in isoform 2 and isoform 3. 4 Publications | 1 | |
Alternative sequenceiVSP_013407 | 689 – 703 | QFYSN…NAGGG → DFFTDCYGYHDFGAS in isoform 2. 2 PublicationsAdd BLAST | 15 | |
Alternative sequenceiVSP_013408 | 704 – 898 | Missing in isoform 2. 2 PublicationsAdd BLAST | 195 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF506968 Genomic DNA Translation: AAM34210.1 AF098967 mRNA Translation: AAC71052.1 Frameshift. AF497751 mRNA Translation: AAO23054.1 AF497752 mRNA Translation: AAO23055.1 AK048096 mRNA Translation: BAC33239.1 AK077560 mRNA Translation: BAC36863.1 Frameshift. AK088604 mRNA Translation: BAC40448.1 BC047272 mRNA Translation: AAH47272.1 |
CCDSi | CCDS40552.1 [Q9Z1X4-1] CCDS90512.1 [Q9Z1X4-2] CCDS90514.1 [Q9Z1X4-3] |
RefSeqi | NP_001036172.1, NM_001042707.2 [Q9Z1X4-1] NP_001036173.1, NM_001042708.2 [Q9Z1X4-2] NP_001264250.1, NM_001277321.1 NP_001264251.1, NM_001277322.1 [Q9Z1X4-3] NP_034691.2, NM_010561.3 [Q9Z1X4-3] XP_006510094.1, XM_006510031.2 [Q9Z1X4-1] |
Genome annotation databases
Ensembli | ENSMUST00000067646; ENSMUSP00000065770; ENSMUSG00000032178 [Q9Z1X4-1] ENSMUST00000213603; ENSMUSP00000149483; ENSMUSG00000032178 [Q9Z1X4-3] ENSMUST00000214758; ENSMUSP00000151032; ENSMUSG00000032178 [Q9Z1X4-3] ENSMUST00000216892; ENSMUSP00000149786; ENSMUSG00000032178 [Q9Z1X4-2] |
GeneIDi | 16201 |
KEGGi | mmu:16201 |
UCSCi | uc009old.2, mouse [Q9Z1X4-3] uc009ole.2, mouse [Q9Z1X4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF506968 Genomic DNA Translation: AAM34210.1 AF098967 mRNA Translation: AAC71052.1 Frameshift. AF497751 mRNA Translation: AAO23054.1 AF497752 mRNA Translation: AAO23055.1 AK048096 mRNA Translation: BAC33239.1 AK077560 mRNA Translation: BAC36863.1 Frameshift. AK088604 mRNA Translation: BAC40448.1 BC047272 mRNA Translation: AAH47272.1 |
CCDSi | CCDS40552.1 [Q9Z1X4-1] CCDS90512.1 [Q9Z1X4-2] CCDS90514.1 [Q9Z1X4-3] |
RefSeqi | NP_001036172.1, NM_001042707.2 [Q9Z1X4-1] NP_001036173.1, NM_001042708.2 [Q9Z1X4-2] NP_001264250.1, NM_001277321.1 NP_001264251.1, NM_001277322.1 [Q9Z1X4-3] NP_034691.2, NM_010561.3 [Q9Z1X4-3] XP_006510094.1, XM_006510031.2 [Q9Z1X4-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4AT7 | X-ray | 1.90 | B | 1-381 | [»] | |
4AT8 | X-ray | 2.69 | B/D | 1-381 | [»] | |
4AT9 | X-ray | 2.80 | B | 1-381 | [»] | |
4ATB | X-ray | 3.10 | B/D | 1-381 | [»] | |
5DV7 | X-ray | 3.50 | C | 4-701 | [»] | |
SMRi | Q9Z1X4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 200648, 59 interactors |
IntActi | Q9Z1X4, 4 interactors |
MINTi | Q9Z1X4 |
STRINGi | 10090.ENSMUSP00000065770 |
PTM databases
iPTMneti | Q9Z1X4 |
PhosphoSitePlusi | Q9Z1X4 |
Proteomic databases
EPDi | Q9Z1X4 |
jPOSTi | Q9Z1X4 |
PaxDbi | Q9Z1X4 |
PeptideAtlasi | Q9Z1X4 |
PRIDEi | Q9Z1X4 |
Protocols and materials databases
Antibodypediai | 1051, 330 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000067646; ENSMUSP00000065770; ENSMUSG00000032178 [Q9Z1X4-1] ENSMUST00000213603; ENSMUSP00000149483; ENSMUSG00000032178 [Q9Z1X4-3] ENSMUST00000214758; ENSMUSP00000151032; ENSMUSG00000032178 [Q9Z1X4-3] ENSMUST00000216892; ENSMUSP00000149786; ENSMUSG00000032178 [Q9Z1X4-2] |
GeneIDi | 16201 |
KEGGi | mmu:16201 |
UCSCi | uc009old.2, mouse [Q9Z1X4-3] uc009ole.2, mouse [Q9Z1X4-1] |
Organism-specific databases
CTDi | 3609 |
MGIi | MGI:1339973, Ilf3 |
Phylogenomic databases
eggNOGi | KOG3792, Eukaryota |
GeneTreei | ENSGT00940000156719 |
InParanoidi | Q9Z1X4 |
OMAi | YSHGQGN |
OrthoDBi | 612611at2759 |
PhylomeDBi | Q9Z1X4 |
TreeFami | TF320194 |
Miscellaneous databases
BioGRID-ORCSi | 16201, 6 hits in 17 CRISPR screens |
ChiTaRSi | Ilf3, mouse |
PROi | PR:Q9Z1X4 |
RNActi | Q9Z1X4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032178, Expressed in undifferentiated genital tubercle and 355 other tissues |
ExpressionAtlasi | Q9Z1X4, baseline and differential |
Genevisiblei | Q9Z1X4, MM |
Family and domain databases
CDDi | cd00048, DSRM, 2 hits |
DisProti | DP01562 |
Gene3Di | 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR014720, dsRBD_dom IPR006561, DZF_dom IPR033099, ILF3/NF90 IPR043519, NT_sf |
PANTHERi | PTHR45762:SF4, PTHR45762:SF4, 3 hits |
Pfami | View protein in Pfam PF00035, dsrm, 2 hits PF07528, DZF, 1 hit |
SMARTi | View protein in SMART SM00358, DSRM, 2 hits SM00572, DZF, 1 hit |
PROSITEi | View protein in PROSITE PS50137, DS_RBD, 2 hits PS51703, DZF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ILF3_MOUSE | |
Accessioni | Q9Z1X4Primary (citable) accession number: Q9Z1X4 Secondary accession number(s): Q80VD5 Q8K588 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 31, 2002 |
Last sequence update: | April 12, 2005 | |
Last modified: | February 10, 2021 | |
This is version 176 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references