UniProtKB - Q9Z1X1 (ESYT1_RAT)
Extended synaptotagmin-1
Esyt1
Functioni
Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (By similarity).
Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane (By similarity).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 336 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 337 | Calcium 1By similarity | 1 | |
Metal bindingi | 337 | Calcium 2By similarity | 1 | |
Metal bindingi | 349 | Calcium 2By similarity | 1 | |
Metal bindingi | 396 | Calcium 1By similarity | 1 | |
Metal bindingi | 396 | Calcium 2By similarity | 1 | |
Metal bindingi | 398 | Calcium 1By similarity | 1 | |
Metal bindingi | 398 | Calcium 2By similarity | 1 | |
Metal bindingi | 398 | Calcium 3; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 400 | Calcium 3; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 402 | Calcium 3By similarity | 1 | |
Metal bindingi | 403 | Calcium 1By similarity | 1 |
GO - Molecular functioni
- calcium-dependent phospholipid binding Source: GO_Central
- calcium ion binding Source: GO_Central
- identical protein binding Source: RGD
- phosphatidylcholine binding Source: GO_Central
- phosphatidylethanolamine binding Source: GO_Central
- phosphatidylinositol binding Source: GO_Central
GO - Biological processi
- endoplasmic reticulum-plasma membrane tethering Source: InterPro
- lipid transport Source: UniProtKB-KW
Keywordsi
Biological process | Lipid transport, Transport |
Ligand | Calcium, Lipid-binding, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-1660662, Glycosphingolipid metabolism R-RNO-9013149, RAC1 GTPase cycle R-RNO-9013404, RAC2 GTPase cycle R-RNO-9013405, RHOD GTPase cycle R-RNO-9013408, RHOG GTPase cycle R-RNO-9013423, RAC3 GTPase cycle R-RNO-9035034, RHOF GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Extended synaptotagmin-1Short name: E-Syt1 Alternative name(s): Membrane-bound C2 domain-containing protein vp115 |
Gene namesi | Name:Esyt1 Synonyms:Fam62a, Mbc2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3053, Esyt1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
Note: Localizes primarily to the endoplasmic reticulum. Recruited to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels.By similarity
Endoplasmic reticulum
- integral component of endoplasmic reticulum membrane Source: UniProtKB
- intrinsic component of endoplasmic reticulum membrane Source: GO_Central
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 30 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 31 – 51 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 52 – 54 | LumenalSequence analysis | 3 | |
Transmembranei | 55 – 75 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 76 – 1088 | CytoplasmicSequence analysisAdd BLAST | 1013 |
Keywords - Cellular componenti
Cell membrane, Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000234347 | 1 – 1088 | Extended synaptotagmin-1Add BLAST | 1088 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 316 | Phosphoserine; by CDK5By similarity | 1 | |
Modified residuei | 806 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 809 | PhosphoserineBy similarity | 1 | |
Modified residuei | 933 | PhosphoserineBy similarity | 1 | |
Modified residuei | 947 | PhosphoserineBy similarity | 1 | |
Modified residuei | 993 | PhosphotyrosineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q9Z1X1 |
PaxDbi | Q9Z1X1 |
PRIDEi | Q9Z1X1 |
PTM databases
iPTMneti | Q9Z1X1 |
PhosphoSitePlusi | Q9Z1X1 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Interacts with ESYT2 and ESYT3.
Interacts (phosphorylated form) with SLC2A4 (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248212, 2 interactors |
IntActi | Q9Z1X1, 1 interactor |
STRINGi | 10116.ENSRNOP00000006119 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 127 – 305 | SMP-LTDPROSITE-ProRule annotationAdd BLAST | 179 | |
Domaini | 304 – 425 | C2 1PROSITE-ProRule annotationAdd BLAST | 122 | |
Domaini | 446 – 572 | C2 2PROSITE-ProRule annotationAdd BLAST | 127 | |
Domaini | 618 – 740 | C2 3PROSITE-ProRule annotationAdd BLAST | 123 | |
Domaini | 771 – 888 | C2 4PROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 955 – 1077 | C2 5PROSITE-ProRule annotationAdd BLAST | 123 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 38 | DisorderedSequence analysisAdd BLAST | 38 | |
Regioni | 599 – 630 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 911 – 930 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 1002 – 1009 | Required for phosphatidylinositol 4,5-bisphosphate-dependent location at the cell membraneBy similarity | 8 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 606 – 630 | Polar residuesSequence analysisAdd BLAST | 25 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1012, Eukaryota |
InParanoidi | Q9Z1X1 |
OrthoDBi | 52746at2759 |
PhylomeDBi | Q9Z1X1 |
TreeFami | TF324255 |
Family and domain databases
CDDi | cd08391, C2A_C2C_Synaptotagmin_like, 2 hits cd04050, C2B_Synaptotagmin-like, 2 hits cd04030, C2C_KIAA1228, 1 hit |
Gene3Di | 2.60.40.150, 5 hits |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR037752, C2C_KIAA1228 IPR037733, Ext_Synaptotagmin_C2A IPR037749, Ext_Synaptotagmin_C2B IPR031468, SMP_LBD IPR039010, Synaptotagmin_SMP |
Pfami | View protein in Pfam PF00168, C2, 5 hits PF17047, SMP_LBD, 1 hit |
PRINTSi | PR00360, C2DOMAIN |
SMARTi | View protein in SMART SM00239, C2, 5 hits |
SUPFAMi | SSF49562, SSF49562, 5 hits |
PROSITEi | View protein in PROSITE PS50004, C2, 5 hits PS51847, SMP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MERSPEEGAG PEPSGQSPAT DSTRERDGGS GVPPAGPGAA SEALAVLTSF
60 70 80 90 100
GRRLLVLVPV YLAGAAGLSV GFVLFGLALY LGWRRVRDGK ERSLRAARQL
110 120 130 140 150
LDDEERITAE TLYMSHRELP AWVSFPDVEK AEWLNKIVVQ VWPFLGQYME
160 170 180 190 200
KLLAETVAPA VRGANPHLQT FTFTRVELGE KPVRIIGVKV HPSQRKDQIL
210 220 230 240 250
LDLNVSYVGD LQIDVEVKKY FCKAGVKGMQ LHGVLRVILE PLIGDLPIVG
260 270 280 290 300
AVSMFFIKRP TLDINWTGMT NLLDIPGLSS LSDTMIMDSI AAFLVLPNRL
310 320 330 340 350
LVPLVPDLQD VAQLRSPLPR GIIRIHLLAA RGLSSKDKYV KGLIEGKSDP
360 370 380 390 400
YALVRVGTQT FCSRVIDEEL NPHWGETYEV IVHEVPGQEI EVEVFDKDPD
410 420 430 440 450
KDDFLGRMKL DVGKVLQAGV LDNWYPLQGG QGQVHLRLEW LSLLPDAEKL
460 470 480 490 500
DQVLQWNRGI TSRPEPPSAA ILVVYLDRAQ DLPLKKGNKE PNPMVQLSVQ
510 520 530 540 550
DVTQESKATY STNCPVWEEA FRFFLQDPRS QELDVQVKDD SRALTLGALT
560 570 580 590 600
LPLARLLTAS ELTLDQWFQL SSSGPNSRLY MKLVMRILYL DSSEMRLPTE
610 620 630 640 650
PGAQDWDSES PETGSSVDAP PRPYHTTPNS HFGTENVLRI HVLEAQDLIA
660 670 680 690 700
KDRFLGGLVK GKSDPYVKLK VAGRSLRTHV VREDLNPRWN EVFEVIVTSI
710 720 730 740 750
PGQELDIEVF DKDLDKDDFL GRYKVGLTTV LNSGFLDEWL TLEDVPSGRL
760 770 780 790 800
HLRLERLSPR PTAAELEEVL QVNSLIQTQK SSELAAALLS VYLERSEDLP
810 820 830 840 850
LRKGTKPPSP YAILTVGETS HKTKTVSQTS APIWEESASF LIRKPHAESL
860 870 880 890 900
ELQVRGEGTG TLGSISLPLS ELLQEEQLCL DRWFALSGQG QVLMRVQLGI
910 920 930 940 950
LVSQHSGVEA HSHSSSSLNE EPEVLGDPTH TASPVLEVRH RLTHGDSPSE
960 970 980 990 1000
ALIGPLGQVK LTVWYHSDEQ KLISIIHSCR ALRQNGRDLP DPYVSVLLLP
1010 1020 1030 1040 1050
DKNRGTKRKT SQKKRTLNPE FNERFEWDLP LDGTLRRKLD VSVKSNSSFM
1060 1070 1080
SRERELLGKV QLDLAEIDLS QGAAQWYDLI DDRDKGGS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JXJ7 | A0A0G2JXJ7_RAT | Extended synaptotagmin-1 | Esyt1 | 1,089 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 373 | H → R in AAI01858 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF099138 mRNA Translation: AAD10051.1 BC101857 mRNA Translation: AAI01858.1 |
RefSeqi | NP_058945.2, NM_017249.2 |
Genome annotation databases
GeneIDi | 29579 |
KEGGi | rno:29579 |
UCSCi | RGD:3053, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF099138 mRNA Translation: AAD10051.1 BC101857 mRNA Translation: AAI01858.1 |
RefSeqi | NP_058945.2, NM_017249.2 |
3D structure databases
SMRi | Q9Z1X1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248212, 2 interactors |
IntActi | Q9Z1X1, 1 interactor |
STRINGi | 10116.ENSRNOP00000006119 |
PTM databases
iPTMneti | Q9Z1X1 |
PhosphoSitePlusi | Q9Z1X1 |
Proteomic databases
jPOSTi | Q9Z1X1 |
PaxDbi | Q9Z1X1 |
PRIDEi | Q9Z1X1 |
Genome annotation databases
GeneIDi | 29579 |
KEGGi | rno:29579 |
UCSCi | RGD:3053, rat |
Organism-specific databases
CTDi | 23344 |
RGDi | 3053, Esyt1 |
Phylogenomic databases
eggNOGi | KOG1012, Eukaryota |
InParanoidi | Q9Z1X1 |
OrthoDBi | 52746at2759 |
PhylomeDBi | Q9Z1X1 |
TreeFami | TF324255 |
Enzyme and pathway databases
Reactomei | R-RNO-1660662, Glycosphingolipid metabolism R-RNO-9013149, RAC1 GTPase cycle R-RNO-9013404, RAC2 GTPase cycle R-RNO-9013405, RHOD GTPase cycle R-RNO-9013408, RHOG GTPase cycle R-RNO-9013423, RAC3 GTPase cycle R-RNO-9035034, RHOF GTPase cycle |
Miscellaneous databases
PROi | PR:Q9Z1X1 |
Family and domain databases
CDDi | cd08391, C2A_C2C_Synaptotagmin_like, 2 hits cd04050, C2B_Synaptotagmin-like, 2 hits cd04030, C2C_KIAA1228, 1 hit |
Gene3Di | 2.60.40.150, 5 hits |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR037752, C2C_KIAA1228 IPR037733, Ext_Synaptotagmin_C2A IPR037749, Ext_Synaptotagmin_C2B IPR031468, SMP_LBD IPR039010, Synaptotagmin_SMP |
Pfami | View protein in Pfam PF00168, C2, 5 hits PF17047, SMP_LBD, 1 hit |
PRINTSi | PR00360, C2DOMAIN |
SMARTi | View protein in SMART SM00239, C2, 5 hits |
SUPFAMi | SSF49562, SSF49562, 5 hits |
PROSITEi | View protein in PROSITE PS50004, C2, 5 hits PS51847, SMP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ESYT1_RAT | |
Accessioni | Q9Z1X1Primary (citable) accession number: Q9Z1X1 Secondary accession number(s): Q3T1L3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | May 1, 1999 | |
Last modified: | September 29, 2021 | |
This is version 128 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families