UniProtKB - Q9Z1W4 (GDF11_MOUSE)
Protein
Growth/differentiation factor 11
Gene
Gdf11
Organism
Mus musculus (Mouse)
Status
Functioni
Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development (PubMed:10391213). May play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3 (PubMed:16845371, PubMed:12414726).By similarity3 Publications
GO - Molecular functioni
- cytokine activity Source: GO_Central
- growth factor activity Source: UniProtKB-KW
GO - Biological processi
- animal organ morphogenesis Source: MGI
- anterior/posterior pattern specification Source: MGI
- camera-type eye morphogenesis Source: MGI
- cell maturation Source: MGI
- metanephros development Source: MGI
- negative regulation of cell differentiation Source: MGI
- negative regulation of cell population proliferation Source: MGI
- negative regulation of neuron differentiation Source: MGI
- pancreas development Source: MGI
- positive regulation of pathway-restricted SMAD protein phosphorylation Source: GO_Central
- roof of mouth development Source: MGI
- skeletal system development Source: MGI
- SMAD protein signal transduction Source: UniProtKB
- spinal cord anterior/posterior patterning Source: MGI
- ureteric bud development Source: MGI
Keywordsi
Molecular function | Cytokine, Growth factor |
Names & Taxonomyi
Protein namesi | Recommended name: Growth/differentiation factor 11Short name: GDF-11 Alternative name(s): Bone morphogenetic protein 11 Short name: BMP-11 |
Gene namesi | Name:Gdf11 Synonyms:Bmp11 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1338027, Gdf11 |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Extracellular region or secreted
- extracellular space Source: MGI
Nucleus
- nucleoplasm Source: MGI
Other locations
- intracellular membrane-bounded organelle Source: MGI
- protein-containing complex Source: MGI
Keywords - Cellular componenti
SecretedPathology & Biotechi
Disruption phenotypei
Deficient mice die neonatally showing altered patterning of the axial skeleton and impaired renal, palate, stomach, spleen and pancreatic development.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 120 | D → A: Inhibits processing of prodomain. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
PropeptideiPRO_0000033988 | 21 – 296 | By similarityAdd BLAST | 276 | |
ChainiPRO_0000033989 | 297 – 405 | Growth/differentiation factor 11Add BLAST | 109 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 92 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 302 ↔ 312 | By similarity | ||
Disulfide bondi | 311 ↔ 370 | By similarity | ||
Disulfide bondi | 339 ↔ 402 | By similarity | ||
Disulfide bondi | 343 ↔ 404 | By similarity | ||
Disulfide bondi | 369 | InterchainBy similarity |
Post-translational modificationi
Synthesized as large precursor molecule that undergoes proteolytic cleavage. The mature C-terminal portion of the molecule is bound non-covalently to its N-terminal propeptide rendering it inactive. Ligand activation requires additional cleavage of the prodomain by a tolloid-like metalloproteinase.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 119 – 120 | Cleavage1 Publication | 2 |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q9Z1W4 |
PeptideAtlasi | Q9Z1W4 |
PRIDEi | Q9Z1W4 |
PTM databases
GlyGeni | Q9Z1W4, 1 site |
iPTMneti | Q9Z1W4 |
PhosphoSitePlusi | Q9Z1W4 |
Expressioni
Tissue specificityi
Highly expressed in the developing limb bud, initially detected in the distal mesenchyme, and later localizing to regions around the developing bones. Is also expressed in adult dental pulp and brain.
Developmental stagei
First strongly expressed in restricted domains at 8.5 dpc where it is highest in the tail bud. At 10.5 dpc, expressed in the branchial arches, limb bud, tail bud and posterior dorsal neural tube. Later, expressed in terminally-differentiated odontoblasts, the nasal epithelium, retina and specific regions of the brain.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000025352, Expressed in dorsal root ganglion and 95 other tissues |
Interactioni
Subunit structurei
Homodimer; disulfide-linked (By similarity).
Interacts directly with ACVR2B (PubMed:16845371, PubMed:12414726).
Interacts directly with ACVR2A (PubMed:12414726).
Interacts with ACVR1B, TGFBR1 and ACVR1C in an ACVR2B-dependent manner (PubMed:16845371).
Interacts with FST isoform 2/FS288 (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- cytokine activity Source: GO_Central
- growth factor activity Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 199886, 3 interactors |
DIPi | DIP-29907N |
IntActi | Q9Z1W4, 5 interactors |
MINTi | Q9Z1W4 |
STRINGi | 10090.ENSMUSP00000026408 |
Miscellaneous databases
RNActi | Q9Z1W4, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 29 – 39 | Poly-AlaAdd BLAST | 11 | |
Compositional biasi | 208 – 213 | Poly-Gly | 6 |
Sequence similaritiesi
Belongs to the TGF-beta family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3900, Eukaryota |
GeneTreei | ENSGT00940000161052 |
HOGENOMi | CLU_020515_6_1_1 |
InParanoidi | Q9Z1W4 |
OMAi | GAHECSA |
OrthoDBi | 892873at2759 |
PhylomeDBi | Q9Z1W4 |
TreeFami | TF318514 |
Family and domain databases
Gene3Di | 2.10.90.10, 1 hit |
InterProi | View protein in InterPro IPR029034, Cystine-knot_cytokine IPR001839, TGF-b_C IPR001111, TGF-b_propeptide IPR015615, TGF-beta-rel IPR017948, TGFb_CS |
PANTHERi | PTHR11848, PTHR11848, 1 hit |
Pfami | View protein in Pfam PF00019, TGF_beta, 1 hit PF00688, TGFb_propeptide, 1 hit |
SMARTi | View protein in SMART SM00204, TGFB, 1 hit |
SUPFAMi | SSF57501, SSF57501, 1 hit |
PROSITEi | View protein in PROSITE PS00250, TGF_BETA_1, 1 hit PS51362, TGF_BETA_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9Z1W4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVLAAPLLLG FLLLALELRP RGEAAEGPAA AAAAAAAAAG VGGERSSRPA
60 70 80 90 100
PSAPPEPDGC PVCVWRQHSR ELRLESIKSQ ILSKLRLKEA PNISREVVKQ
110 120 130 140 150
LLPKAPPLQQ ILDLHDFQGD ALQPEDFLEE DEYHATTETV ISMAQETDPA
160 170 180 190 200
VQTDGSPLCC HFHFSPKVMF TKVLKAQLWV YLRPVPRPAT VYLQILRLKP
210 220 230 240 250
LTGEGTAGGG GGGRRHIRIR SLKIELHSRS GHWQSIDFKQ VLHSWFRQPQ
260 270 280 290 300
SNWGIEINAF DPSGTDLAVT SLGPGAEGLH PFMELRVLEN TKRSRRNLGL
310 320 330 340 350
DCDEHSSESR CCRYPLTVDF EAFGWDWIIA PKRYKANYCS GQCEYMFMQK
360 370 380 390 400
YPHTHLVQQA NPRGSAGPCC TPTKMSPINM LYFNDKQQII YGKIPGMVVD
RCGCS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 75 | E → G in AAD05267 (PubMed:10072786).Curated | 1 | |
Sequence conflicti | 171 | T → N in AAF21633 (PubMed:10391213).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF100906, AF100904, AF100905 Genomic DNA Translation: AAC72853.1 AF028337, AF028335, AF028336 Genomic DNA Translation: AAF21633.1 AF092734 mRNA Translation: AAD05267.1 |
CCDSi | CCDS24296.1 |
RefSeqi | NP_034402.1, NM_010272.2 |
Genome annotation databases
Ensembli | ENSMUST00000026408; ENSMUSP00000026408; ENSMUSG00000025352 |
GeneIDi | 14561 |
KEGGi | mmu:14561 |
UCSCi | uc007hot.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF100906, AF100904, AF100905 Genomic DNA Translation: AAC72853.1 AF028337, AF028335, AF028336 Genomic DNA Translation: AAF21633.1 AF092734 mRNA Translation: AAD05267.1 |
CCDSi | CCDS24296.1 |
RefSeqi | NP_034402.1, NM_010272.2 |
3D structure databases
SMRi | Q9Z1W4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199886, 3 interactors |
DIPi | DIP-29907N |
IntActi | Q9Z1W4, 5 interactors |
MINTi | Q9Z1W4 |
STRINGi | 10090.ENSMUSP00000026408 |
PTM databases
GlyGeni | Q9Z1W4, 1 site |
iPTMneti | Q9Z1W4 |
PhosphoSitePlusi | Q9Z1W4 |
Proteomic databases
PaxDbi | Q9Z1W4 |
PeptideAtlasi | Q9Z1W4 |
PRIDEi | Q9Z1W4 |
Protocols and materials databases
Antibodypediai | 27725, 274 antibodies |
DNASUi | 14561 |
Genome annotation databases
Ensembli | ENSMUST00000026408; ENSMUSP00000026408; ENSMUSG00000025352 |
GeneIDi | 14561 |
KEGGi | mmu:14561 |
UCSCi | uc007hot.1, mouse |
Organism-specific databases
CTDi | 10220 |
MGIi | MGI:1338027, Gdf11 |
Phylogenomic databases
eggNOGi | KOG3900, Eukaryota |
GeneTreei | ENSGT00940000161052 |
HOGENOMi | CLU_020515_6_1_1 |
InParanoidi | Q9Z1W4 |
OMAi | GAHECSA |
OrthoDBi | 892873at2759 |
PhylomeDBi | Q9Z1W4 |
TreeFami | TF318514 |
Miscellaneous databases
BioGRID-ORCSi | 14561, 0 hits in 17 CRISPR screens |
PROi | PR:Q9Z1W4 |
RNActi | Q9Z1W4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025352, Expressed in dorsal root ganglion and 95 other tissues |
Family and domain databases
Gene3Di | 2.10.90.10, 1 hit |
InterProi | View protein in InterPro IPR029034, Cystine-knot_cytokine IPR001839, TGF-b_C IPR001111, TGF-b_propeptide IPR015615, TGF-beta-rel IPR017948, TGFb_CS |
PANTHERi | PTHR11848, PTHR11848, 1 hit |
Pfami | View protein in Pfam PF00019, TGF_beta, 1 hit PF00688, TGFb_propeptide, 1 hit |
SMARTi | View protein in SMART SM00204, TGFB, 1 hit |
SUPFAMi | SSF57501, SSF57501, 1 hit |
PROSITEi | View protein in PROSITE PS00250, TGF_BETA_1, 1 hit PS51362, TGF_BETA_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GDF11_MOUSE | |
Accessioni | Q9Z1W4Primary (citable) accession number: Q9Z1W4 Secondary accession number(s): Q9QX55, Q9R221 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | May 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families