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Protein

AP-3 complex subunit beta-1

Gene

Ap3b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-432722 Golgi Associated Vesicle Biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit beta-1
Alternative name(s):
Adaptor protein complex AP-3 subunit beta-1
Adaptor-related protein complex 3 subunit beta-1
Beta-3A-adaptin
Clathrin assembly protein complex 3 beta-1 large chain
Gene namesi
Name:Ap3b1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1333879 Ap3b1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Ap3b1 are the cause of the autosomal recessive phenotype 'pearl' (pe). Pearl mice exhibit hypopigmentation, lysosomal secretion abnormalities, and platelet-dense granules with reduced levels of adenine nucleotides and serotonin. The changes in platelets lead to prolonged bleeding. Additionally, pearl mice exhibit reduced sensitivity in the dark-adapted state (PubMed:9931340).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001937471 – 1105AP-3 complex subunit beta-1Add BLAST1105

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei276PhosphoserineCombined sources1
Modified residuei610PhosphoserineBy similarity1
Modified residuei761PhosphoserineBy similarity1
Modified residuei763PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Z1T1
MaxQBiQ9Z1T1
PaxDbiQ9Z1T1
PeptideAtlasiQ9Z1T1
PRIDEiQ9Z1T1

PTM databases

iPTMnetiQ9Z1T1
PhosphoSitePlusiQ9Z1T1

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSMUSG00000021686 Expressed in 293 organ(s), highest expression level in testis
CleanExiMM_AP3B1
GenevisibleiQ9Z1T1 MM

Interactioni

Subunit structurei

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2). AP-3 associates with the BLOC-1 complex.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198132, 1 interactor
CORUMiQ9Z1T1
DIPiDIP-49018N
IntActiQ9Z1T1, 9 interactors
MINTiQ9Z1T1
STRINGi10090.ENSMUSP00000022196

Structurei

3D structure databases

ProteinModelPortaliQ9Z1T1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi678 – 802Glu/Ser-richAdd BLAST125

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1060 Eukaryota
COG5096 LUCA
GeneTreeiENSGT00530000063546
HOGENOMiHOG000033978
HOVERGENiHBG050519
InParanoidiQ9Z1T1
KOiK12397
OMAiWHLAPKS
OrthoDBiEOG091G01OB
TreeFamiTF314605

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR026740 AP3_beta
IPR029394 AP3B1_Ser
IPR029390 AP3B_C
IPR026739 AP_beta
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR015151 B-adaptin_app_sub_C
IPR002553 Clathrin/coatomer_adapt-like_N
PANTHERiPTHR11134 PTHR11134, 1 hit
PfamiView protein in Pfam
PF01602 Adaptin_N, 1 hit
PF14796 AP3B1_C, 1 hit
PF14797 SEEEED, 1 hit
PIRSFiPIRSF037096 AP3_complex_beta, 1 hit
SMARTiView protein in SMART
SM01355 AP3B1_C, 1 hit
SM01020 B2-adapt-app_C, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9Z1T1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSNSFAYNE QSGGGEAAEL GQEATSTISP SGAFGLFSSD WKKNEDLKQM
60 70 80 90 100
LESNKDSAKL DAMKRIVGMI AKGKNASELF PAVVKNVASK NIEIKKLVYV
110 120 130 140 150
YLVRYAEEQQ DLALLSISTF QRALKDPNQL IRASALRVLS SIRVPIIVPV
160 170 180 190 200
MMLAIKEASA DLSPYVRKNA AHAIQKLYSL DPEQKEMLIE VIEKLLKDKS
210 220 230 240 250
TLVAGSVVMA FEEVCPDRID LIHRNYRKLC NLLVDVEEWG QVVIIHMLTR
260 270 280 290 300
YARTQFVSPW REDGGLEDNE KNFYESEEEE EEKEKSSRKK SYAMDPDHRL
310 320 330 340 350
LIRNTKPLLQ SRNAAVVMAV AQLYWHISPK SEAGVISKSL VRLLRSNREV
360 370 380 390 400
QYIVLQNIAT MSIERKGMFE PYLKSFYVRS TDPTMIKTLK LEILTNLANE
410 420 430 440 450
ANISTLLREF QTYVRSQDKQ FAAATIQTIG RCATSISEVT DTCLNGLVCL
460 470 480 490 500
LSNRDEIVVA ESVVVIKKLL QMQPAQHGEI IRHMAKLLDS ITVPVARASI
510 520 530 540 550
LWLIGENCER VPKIAPDVLR KMAKSFTSED DLVKLQILNL AAKLYLTNSK
560 570 580 590 600
QTKLLTQYIL NLGKYDQNYD IRDRTRFIRQ LIVPNEKSGA LSKYAKKIFL
610 620 630 640 650
APKPAPLLES PFKDRDRFQL GTLSHTLNIK ASGYLELSNW PEVAPDPSVR
660 670 680 690 700
NVEVIESAKE WTPLGKTKKE KPMKKFYSES EEEEDEDEDE DEEEEEKEDE
710 720 730 740 750
DENPSDSSSD SESGSGSESG DTGTEDSSED SSSGQDSETG SQAEAERQKV
760 770 780 790 800
AKRNSKTKRK SDSENREKKN ENSKASESSS EESSSMEDSS SESESESGSD
810 820 830 840 850
SEPAPRNVAP AKERKPQQER HPPSKDVFLL DLDDFNPVST PVALPTPALS
860 870 880 890 900
PSLIADLEGL NLSTSSSVIN VSTPVFVPTK THELLHRMHG KGLAAHYCFP
910 920 930 940 950
RQPCIFSDKM VSVQITLTNT SDRKIENIHI GGKGLPVGMQ MHAFHPIDSL
960 970 980 990 1000
EPKGSVTVSV GIDFCDSTQT ASFQLCTKDD CFNVTLQPPV GELLSPVAMS
1010 1020 1030 1040 1050
EKDFKKEQGT LTGMNETSAT LIAAPQNFTP SMILQKVVNV ANLGAVPSSQ
1060 1070 1080 1090 1100
DNVHRFAART VHSGSLMLVT VELKEGSTAQ LIINTEKTVI GSVLLRELKP

VLSQG
Length:1,105
Mass (Da):122,740
Last modified:July 27, 2011 - v2
Checksum:i59EB4A722B40E233
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A338P6V5A0A338P6V5_MOUSE
AP-3 complex subunit beta-1
Ap3b1
484Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti275 – 277ESE → DS in AAH15068 (PubMed:15489334).Curated3
Sequence conflicti439 – 444VTDTCL → FTETCF in AAC78338 (PubMed:9931340).Curated6
Sequence conflicti439 – 444VTDTCL → FTETCF in AAG23622 (PubMed:11056055).Curated6
Sequence conflicti487L → F in AAC78338 (PubMed:9931340).Curated1
Sequence conflicti487L → F in AAG23622 (PubMed:11056055).Curated1
Sequence conflicti691D → DEE in AAH15068 (PubMed:15489334).Curated1
Sequence conflicti714G → E in AAH15068 (PubMed:15489334).Curated1
Sequence conflicti724T → TSN in AAH15068 (PubMed:15489334).Curated1
Sequence conflicti737S → N in AAH15068 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF103809 mRNA Translation: AAC78338.1
AF255589
, AF255566, AF255567, AF255568, AF255569, AF255570, AF255571, AF255572, AF255573, AF255574, AF255575, AF255576, AF255577, AF255578, AF255579, AF255580, AF255581, AF255582, AF255583, AF255584, AF255585, AF255586, AF255587, AF255588 Genomic DNA Translation: AAG23622.1
AC122373 Genomic DNA No translation available.
AC123043 Genomic DNA No translation available.
BC015068 mRNA Translation: AAH15068.1
CCDSiCCDS26693.1
PIRiT18295
RefSeqiNP_033810.2, NM_009680.3
UniGeneiMm.21185

Genome annotation databases

EnsembliENSMUST00000022196; ENSMUSP00000022196; ENSMUSG00000021686
GeneIDi11774
KEGGimmu:11774
UCSCiuc007rlv.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF103809 mRNA Translation: AAC78338.1
AF255589
, AF255566, AF255567, AF255568, AF255569, AF255570, AF255571, AF255572, AF255573, AF255574, AF255575, AF255576, AF255577, AF255578, AF255579, AF255580, AF255581, AF255582, AF255583, AF255584, AF255585, AF255586, AF255587, AF255588 Genomic DNA Translation: AAG23622.1
AC122373 Genomic DNA No translation available.
AC123043 Genomic DNA No translation available.
BC015068 mRNA Translation: AAH15068.1
CCDSiCCDS26693.1
PIRiT18295
RefSeqiNP_033810.2, NM_009680.3
UniGeneiMm.21185

3D structure databases

ProteinModelPortaliQ9Z1T1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198132, 1 interactor
CORUMiQ9Z1T1
DIPiDIP-49018N
IntActiQ9Z1T1, 9 interactors
MINTiQ9Z1T1
STRINGi10090.ENSMUSP00000022196

PTM databases

iPTMnetiQ9Z1T1
PhosphoSitePlusiQ9Z1T1

Proteomic databases

EPDiQ9Z1T1
MaxQBiQ9Z1T1
PaxDbiQ9Z1T1
PeptideAtlasiQ9Z1T1
PRIDEiQ9Z1T1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022196; ENSMUSP00000022196; ENSMUSG00000021686
GeneIDi11774
KEGGimmu:11774
UCSCiuc007rlv.2 mouse

Organism-specific databases

CTDi8546
MGIiMGI:1333879 Ap3b1

Phylogenomic databases

eggNOGiKOG1060 Eukaryota
COG5096 LUCA
GeneTreeiENSGT00530000063546
HOGENOMiHOG000033978
HOVERGENiHBG050519
InParanoidiQ9Z1T1
KOiK12397
OMAiWHLAPKS
OrthoDBiEOG091G01OB
TreeFamiTF314605

Enzyme and pathway databases

ReactomeiR-MMU-432722 Golgi Associated Vesicle Biogenesis

Miscellaneous databases

PROiPR:Q9Z1T1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021686 Expressed in 293 organ(s), highest expression level in testis
CleanExiMM_AP3B1
GenevisibleiQ9Z1T1 MM

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR026740 AP3_beta
IPR029394 AP3B1_Ser
IPR029390 AP3B_C
IPR026739 AP_beta
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR015151 B-adaptin_app_sub_C
IPR002553 Clathrin/coatomer_adapt-like_N
PANTHERiPTHR11134 PTHR11134, 1 hit
PfamiView protein in Pfam
PF01602 Adaptin_N, 1 hit
PF14796 AP3B1_C, 1 hit
PF14797 SEEEED, 1 hit
PIRSFiPIRSF037096 AP3_complex_beta, 1 hit
SMARTiView protein in SMART
SM01355 AP3B1_C, 1 hit
SM01020 B2-adapt-app_C, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAP3B1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1T1
Secondary accession number(s): E9QQ08, Q91YR4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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