UniProtKB - Q9Z1R2 (BAG6_MOUSE)
Large proline-rich protein BAG6
Bag6
Functioni
GO - Molecular functioni
- Hsp70 protein binding Source: UniProtKB
- identical protein binding Source: MGI
- misfolded protein binding Source: MGI
- polyubiquitin modification-dependent protein binding Source: UniProtKB
- proteasome binding Source: UniProtKB
- ribosome binding Source: UniProtKB
- signaling receptor binding Source: MGI
- ubiquitin protein ligase binding Source: MGI
- ubiquitin-specific protease binding Source: MGI
GO - Biological processi
- antigen processing and presentation of peptide antigen via MHC class I Source: UniProtKB
- apoptotic process Source: UniProtKB
- brain development Source: UniProtKB
- cell differentiation Source: UniProtKB-KW
- chromatin organization Source: UniProtKB-KW
- endoplasmic reticulum stress-induced pre-emptive quality control Source: UniProtKB
- ER-associated misfolded protein catabolic process Source: UniProtKB
- immune response-activating cell surface receptor signaling pathway Source: MGI
- internal peptidyl-lysine acetylation Source: UniProtKB
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: UniProtKB
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
- kidney development Source: UniProtKB
- lung development Source: UniProtKB
- maintenance of unfolded protein involved in ERAD pathway Source: MGI
- natural killer cell activation Source: MGI
- negative regulation of apoptotic process Source: MGI
- negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- negative regulation of proteolysis Source: UniProtKB
- positive regulation of ERAD pathway Source: MGI
- proteasomal protein catabolic process Source: UniProtKB
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
- protein stabilization Source: UniProtKB
- regulation of apoptotic process Source: UniProtKB
- regulation of cell population proliferation Source: Ensembl
- regulation of embryonic development Source: UniProtKB
- spermatogenesis Source: UniProtKB
- synaptonemal complex assembly Source: UniProtKB
- tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
- ubiquitin-dependent ERAD pathway Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Molecular function | Chaperone, Chromatin regulator |
Biological process | Apoptosis, Differentiation, Immunity, Spermatogenesis, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Large proline-rich protein BAG6CuratedAlternative name(s): BAG family molecular chaperone regulator 6 BCL2-associated athanogene 6Imported Short name: BAG-61 Publication HLA-B-associated transcript 31 Publication Protein Scythe1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919439, Bag6 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Extracellular region or secreted
- extracellular exosome By similarity
Cytosol
- cytosol 1 Publication
Note: Normally localized in cytosol and nucleus, it can also be released extracellularly, in exosomes, by tumor and myeloid dendritic cells.By similarity
Cytosol
- BAT3 complex Source: UniProtKB
- cytosol Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- intracellular membrane-bounded organelle Source: MGI
- membrane Source: MGI
Keywords - Cellular componenti
Cytoplasm, Nucleus, SecretedPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000114898 | 1 – 1154 | Large proline-rich protein BAG6Add BLAST | 1154 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 96 | PhosphoserineBy similarity | 1 | |
Modified residuei | 117 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 986 | PhosphoserineBy similarity | 1 | |
Modified residuei | 995 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1075 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1103 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1139 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 1023 – 1024 | Cleavage; by CASP3By similarity | 2 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9Z1R2 |
jPOSTi | Q9Z1R2 |
MaxQBi | Q9Z1R2 |
PaxDbi | Q9Z1R2 |
PRIDEi | Q9Z1R2 |
PTM databases
iPTMneti | Q9Z1R2 |
PhosphoSitePlusi | Q9Z1R2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024392, Expressed in cerebral cortex and 313 other tissues |
ExpressionAtlasi | Q9Z1R2, baseline and differential |
Genevisiblei | Q9Z1R2, MM |
Interactioni
Subunit structurei
Component of the BAG6/BAT3 complex, also named BAT3 complex, at least composed of BAG6, UBL4A and GET4/TRC35.
Interacts with GET4; the interaction is direct and localizes BAG6 in the cytosol (By similarity).
Interacts with UBL4A; the interaction is direct and required for UBL4A protein stability (By similarity).
Interacts with AIFM1 (PubMed:18056262).
Interacts with HSPA2 (PubMed:18678708).
Interacts with CTCFL.
Interacts with p300/EP300.
Interacts (via ubiquitin-like domain) with RNF126; required for BAG6-dependent ubiquitination of proteins mislocalized to the cytosol.
Interacts (via ubiquitin-like domain) with SGTA; SGTA competes with RNF126 by binding the same region of BAG6, thereby promoting deubiquitination of BAG6-target proteins and rescuing them from degradation.
Interacts with ricin A chain.
Interacts with VCP and AMFR; both form the VCP/p97-AMFR/gp78 complex.
Interacts with SYVN1.
Interacts with USP13; the interaction is direct and may mediate UBL4A deubiquitination (By similarity).
Interacts with ZFAND2B (PubMed:24160817, PubMed:26337389, PubMed:26876100).
Interacts with KPNA2 (By similarity).
Interacts with UBQLN4 (By similarity).
By similarity5 PublicationsBinary interactionsi
Hide detailsQ9Z1R2
With | #Exp. | IntAct |
---|---|---|
Camk2a - isoform Alpha KAP [P11798-2] | 3 | EBI-644645,EBI-400402 |
Tnfrsf11a [O35305] | 4 | EBI-644645,EBI-647362 |
GO - Molecular functioni
- Hsp70 protein binding Source: UniProtKB
- identical protein binding Source: MGI
- misfolded protein binding Source: MGI
- polyubiquitin modification-dependent protein binding Source: UniProtKB
- signaling receptor binding Source: MGI
- ubiquitin protein ligase binding Source: MGI
- ubiquitin-specific protease binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 230309, 19 interactors |
ComplexPortali | CPX-133, BAT3 complex |
DIPi | DIP-49391N |
IntActi | Q9Z1R2, 8 interactors |
MINTi | Q9Z1R2 |
STRINGi | 10090.ENSMUSP00000025250 |
Miscellaneous databases
RNActi | Q9Z1R2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 92 | Ubiquitin-likePROSITE-ProRule annotationAdd BLAST | 76 | |
Repeati | 237 – 271 | 1By similarityAdd BLAST | 35 | |
Repeati | 416 – 444 | 2By similarityAdd BLAST | 29 | |
Repeati | 597 – 624 | 3By similarityAdd BLAST | 28 | |
Repeati | 630 – 658 | 4By similarityAdd BLAST | 29 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 237 – 658 | 4 X 29 AA approximate repeatsBy similarityAdd BLAST | 422 | |
Regioni | 1032 – 1062 | Required for interaction with GET4By similarityAdd BLAST | 31 | |
Regioni | 1044 – 1154 | Sufficient for the delivery of client proteins to the endoplasmic reticulumBy similarityAdd BLAST | 111 | |
Regioni | 1080 – 1137 | BAG-similar domain, required and sufficient for interaction with UBL4ABy similarityAdd BLAST | 58 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1034 – 1076 | Nuclear localization siteBy similarityAdd BLAST | 43 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 196 – 274 | Pro-richPROSITE-ProRule annotationAdd BLAST | 79 | |
Compositional biasi | 395 – 720 | Pro-richPROSITE-ProRule annotationAdd BLAST | 326 | |
Compositional biasi | 564 – 610 | Ala-richAdd BLAST | 47 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG4248, Eukaryota |
GeneTreei | ENSGT00390000016199 |
HOGENOMi | CLU_012159_0_0_1 |
InParanoidi | Q9Z1R2 |
OrthoDBi | 1233552at2759 |
PhylomeDBi | Q9Z1R2 |
TreeFami | TF328437 |
Family and domain databases
InterProi | View protein in InterPro IPR021925, BAG6 IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR019954, Ubiquitin_CS |
Pfami | View protein in Pfam PF12057, BAG6, 1 hit PF00240, ubiquitin, 1 hit |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00299, UBIQUITIN_1, 1 hit PS50053, UBIQUITIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MEPSDSASTA MEEPDSLEVL VKTLDSQTRT FIVGAQMNVK EFKEHIAASV
60 70 80 90 100
SIPSEKQRLI YQGRVLQDDK KLQEYNVGGK VIHLVERAPP QTQLPSGASS
110 120 130 140 150
GTGSASATHG GAPLPGTRGP GASVHDRNAN SYVMVGTFNL PSDGSAVDVH
160 170 180 190 200
INMEQAPIQS EPRVRLVMAQ HMIRDIQTLL SRMECRGGTQ AQASQPPPQT
210 220 230 240 250
PQTVASETVA LNSQTSEPVE SEAPPREPME SEEMEERPPT QTPELAPSGP
260 270 280 290 300
APAGPAPAGP APAPETNAPN HPSPAEHVEV LQELQRLQRR LQPFLQRYCE
310 320 330 340 350
VLGAAATTDY NNNHEGREED QRLINLVGES LRLLGNTFVA LSDLRCNLAC
360 370 380 390 400
APPRHLHVVR PMSHYTTPMV LQQAAIPIQI NVGTTVTMTG NGARPPPAPG
410 420 430 440 450
AEAATPGSAQ ATSLPPSSTT VDSSTEGAPP PGPAPPPASS HPRVIRISHQ
460 470 480 490 500
SVEPVVMMHM NIQDSGAQPG GVPSAPTGPL GPPGHGQTLG QQVPGFPTAP
510 520 530 540 550
TRVVIARPTP PQARPSHPGG PPVSGALQGA GLGTNTSLAQ MVSGLVGQLL
560 570 580 590 600
MQPVLVAQGT PGMAQAQAQA QAQAQAQAQA PAPAPAPAPA PATASASAGT
610 620 630 640 650
TNTATTAGPA PGGPAQPPPP QPSAADLQFS QLLGNLLGPA GPGAGGPGMA
660 670 680 690 700
SPTITVAMPG VPAFLQGMTD FLQASQTAPP PPPPPPPPPP APEQQSTPPP
710 720 730 740 750
GSPSGGTASP GGLGPESLPP EFFTSVVQGV LSSLLGSLGA RAGSSESIAA
760 770 780 790 800
FIQRLSGSSN IFEPGADGAL GFFGALLSLL CQNFSMVDVV MLLHGHFQPL
810 820 830 840 850
QRLQPQLRSF FHQHYLGGQE PTPSNIRMAT HTLITGLEEY VRESFSLVQV
860 870 880 890 900
QPGVDIIRTN LEFLQEQFNS IAAHVLHCTD SGFGARLLEL CNQGLFECLA
910 920 930 940 950
LNLHCLGGQQ MELAAVINGR IRRMSRGVNP SLVSWLTTMM GLRLQVVLEH
960 970 980 990 1000
MPVGPDAILR YVRRVGDPPQ TLPEEPMEVQ GAERTSPEPQ RENASPAPGT
1010 1020 1030 1040 1050
TAEEAMSRGP PPAPEGGSRD EQDGASADAE PWAAAVPPEW VPIIQQDIQS
1060 1070 1080 1090 1100
QRKVKPQPPL SDAYLSGMPA KRRKTMQGEG PQLLLSEAVS RAAKAAGARP
1110 1120 1130 1140 1150
LTSPESLSRD LEAPEVQESY RQQLRSDIQK RLQEDPNYSP QRFPNAHRAF
ADDP
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3UXT8 | G3UXT8_MOUSE | BCL2-associated athanogene 6 | Bag6 | 563 | Annotation score: | ||
Q3UF95 | Q3UF95_MOUSE | BCL2-associated athanogene 6 | Bag6 Bat3 | 1,136 | Annotation score: | ||
G3UZT6 | G3UZT6_MOUSE | BCL2-associated athanogene 6 | Bag6 | 1,122 | Annotation score: | ||
G3UYQ2 | G3UYQ2_MOUSE | BCL2-associated athanogene 6 | Bag6 | 1,082 | Annotation score: | ||
A0A1B0GX81 | A0A1B0GX81_MOUSE | BCL2-associated athanogene 6 | Bag6 | 1,107 | Annotation score: | ||
G3V013 | G3V013_MOUSE | Large proline-rich protein BAG6 | Bag6 | 253 | Annotation score: | ||
S4R183 | S4R183_MOUSE | Large proline-rich protein BAG6 | Bag6 | 115 | Annotation score: | ||
G3UYZ0 | G3UYZ0_MOUSE | Large proline-rich protein BAG6 | Bag6 | 209 | Annotation score: | ||
S4R224 | S4R224_MOUSE | Large proline-rich protein BAG6 | Bag6 | 120 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 528 | Missing in AAH26647 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1012 | P → S in AAH26647 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF109719 Genomic DNA Translation: AAC82479.1 CR974444 Genomic DNA No translation available. BC026647 mRNA Translation: AAH26647.1 |
CCDSi | CCDS28688.1 |
RefSeqi | NP_476512.1, NM_057171.2 |
Genome annotation databases
Ensembli | ENSMUST00000025250; ENSMUSP00000025250; ENSMUSG00000024392 |
GeneIDi | 224727 |
KEGGi | mmu:224727 |
UCSCi | uc008cgb.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF109719 Genomic DNA Translation: AAC82479.1 CR974444 Genomic DNA No translation available. BC026647 mRNA Translation: AAH26647.1 |
CCDSi | CCDS28688.1 |
RefSeqi | NP_476512.1, NM_057171.2 |
3D structure databases
SMRi | Q9Z1R2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230309, 19 interactors |
ComplexPortali | CPX-133, BAT3 complex |
DIPi | DIP-49391N |
IntActi | Q9Z1R2, 8 interactors |
MINTi | Q9Z1R2 |
STRINGi | 10090.ENSMUSP00000025250 |
PTM databases
iPTMneti | Q9Z1R2 |
PhosphoSitePlusi | Q9Z1R2 |
Proteomic databases
EPDi | Q9Z1R2 |
jPOSTi | Q9Z1R2 |
MaxQBi | Q9Z1R2 |
PaxDbi | Q9Z1R2 |
PRIDEi | Q9Z1R2 |
Protocols and materials databases
Antibodypediai | 27346, 283 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000025250; ENSMUSP00000025250; ENSMUSG00000024392 |
GeneIDi | 224727 |
KEGGi | mmu:224727 |
UCSCi | uc008cgb.2, mouse |
Organism-specific databases
CTDi | 7917 |
MGIi | MGI:1919439, Bag6 |
Phylogenomic databases
eggNOGi | KOG4248, Eukaryota |
GeneTreei | ENSGT00390000016199 |
HOGENOMi | CLU_012159_0_0_1 |
InParanoidi | Q9Z1R2 |
OrthoDBi | 1233552at2759 |
PhylomeDBi | Q9Z1R2 |
TreeFami | TF328437 |
Miscellaneous databases
BioGRID-ORCSi | 224727, 3 hits in 19 CRISPR screens |
ChiTaRSi | Bag6, mouse |
PROi | PR:Q9Z1R2 |
RNActi | Q9Z1R2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024392, Expressed in cerebral cortex and 313 other tissues |
ExpressionAtlasi | Q9Z1R2, baseline and differential |
Genevisiblei | Q9Z1R2, MM |
Family and domain databases
InterProi | View protein in InterPro IPR021925, BAG6 IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR019954, Ubiquitin_CS |
Pfami | View protein in Pfam PF12057, BAG6, 1 hit PF00240, ubiquitin, 1 hit |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00299, UBIQUITIN_1, 1 hit PS50053, UBIQUITIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BAG6_MOUSE | |
Accessioni | Q9Z1R2Primary (citable) accession number: Q9Z1R2 Secondary accession number(s): Q8SNA3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2005 |
Last sequence update: | May 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot