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Protein

Protein unc-13 homolog B

Gene

Unc13b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP) (By similarity). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri489 – 539Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processExocytosis
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-181429 Serotonin Neurotransmitter Release Cycle
R-MMU-181430 Norepinephrine Neurotransmitter Release Cycle
R-MMU-210500 Glutamate Neurotransmitter Release Cycle
R-MMU-212676 Dopamine Neurotransmitter Release Cycle
R-MMU-264642 Acetylcholine Neurotransmitter Release Cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Protein unc-13 homolog B
Alternative name(s):
Munc13-2
Short name:
munc13
Gene namesi
Name:Unc13b
Synonyms:Unc13a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1342278 Unc13b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Golgi apparatus, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001885761 – 1602Protein unc-13 homolog BAdd BLAST1602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16PhosphoserineBy similarity1
Modified residuei307PhosphoserineBy similarity1
Modified residuei379PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9Z1N9
PRIDEiQ9Z1N9

PTM databases

iPTMnetiQ9Z1N9
PhosphoSitePlusiQ9Z1N9

Expressioni

Gene expression databases

BgeeiENSMUSG00000028456 Expressed in 255 organ(s), highest expression level in submandibular gland
CleanExiMM_UNC13A
MM_UNC13B
ExpressionAtlasiQ9Z1N9 baseline and differential
GenevisibleiQ9Z1N9 MM

Interactioni

Subunit structurei

Interacts with RIMS1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204444, 1 interactor
STRINGi10090.ENSMUSP00000103586

Structurei

3D structure databases

ProteinModelPortaliQ9Z1N9
SMRiQ9Z1N9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 79C2 1PROSITE-ProRule annotationAdd BLAST79
Domaini599 – 705C2 2PROSITE-ProRule annotationAdd BLAST107
Domaini1024 – 1168MHD1PROSITE-ProRule annotationAdd BLAST145
Domaini1275 – 1417MHD2PROSITE-ProRule annotationAdd BLAST143
Domaini1437 – 1542C2 3PROSITE-ProRule annotationAdd BLAST106

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1210 – 1231Sequence analysisAdd BLAST22

Domaini

The C2 domains are not involved in calcium-dependent phospholipid binding.By similarity

Sequence similaritiesi

Belongs to the unc-13 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri489 – 539Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1011 Eukaryota
ENOG410XS5D LUCA
GeneTreeiENSGT00920000149033
HOGENOMiHOG000231404
HOVERGENiHBG057340
InParanoidiQ9Z1N9
KOiK15293
OMAiKEKMPCI
PhylomeDBiQ9Z1N9
TreeFamiTF312844

Family and domain databases

CDDicd00029 C1, 1 hit
cd04027 C2B_Munc13, 1 hit
Gene3Di2.60.40.150, 3 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR010439 CAPS_dom
IPR014770 Munc13_1
IPR014772 Munc13_dom-2
IPR019558 Munc13_subgr_dom-2
IPR002219 PE/DAG-bd
IPR027080 Unc-13
IPR037302 Unc-13_C2B
PANTHERiPTHR10480 PTHR10480, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF00168 C2, 3 hits
PF06292 DUF1041, 1 hit
PF10540 Membr_traf_MHD, 1 hit
PRINTSiPR00360 C2DOMAIN
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00239 C2, 3 hits
SM01145 DUF1041, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits
PS51258 MHD1, 1 hit
PS51259 MHD2, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Z1N9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSLLCVRVKR AKFQGSPDKF NTYVTLKVQN VKSTTVAVRG DQPSWEQDFM
60 70 80 90 100
FEISRLDLGL SVEVWNKGLI WDTMVGTVWI ALKTIRQSDE EGPGEWSTLE
110 120 130 140 150
AETLMKDDEI CGTKNPTPHK ILLDTRFELP FDIPEEEARY WTYKLEQINA
160 170 180 190 200
LADDNEYSSQ EESQRKPLPT AAAQCCHWTY LGWGEHQTFE DPDSAVDDRD
210 220 230 240 250
SDYRSETSNS APPPYHTTTQ PNASVHQFPV PVRLPQQLFL QGSSHDSCND
260 270 280 290 300
SMQSYDLDYP ERRALSPTSS SRYGSSCNVS QGSSLLSELD QYHEQDDDGR
310 320 330 340 350
ERDSIHSSHS YGSLSKDGQA GLGEQEKALE VTCESEKEKT GESKEMRDDA
360 370 380 390 400
TIHPPSDLVL HKDHVLGPQE SLPEETASSP FTQARAHWFR AVTKVRLQLQ
410 420 430 440 450
EISDDGDPSL PQWLPEGPAG GLYGIDSMPD LRRKKPLPLV SDLSLVQSRK
460 470 480 490 500
AGITSAMATR TSLKDEELKS HVYKKTLQAL IYPISCTTPH NFEVWSATTP
510 520 530 540 550
TYCYECEGLL WGLARQGMRC SECGVKCHEK CQDLLNADCL QRAAEKSSKH
560 570 580 590 600
GAEDRTQNII MAMKDRMKIR ERNKPEIFEV IRDVFTVSKV AHVQQMKTVK
610 620 630 640 650
QSVLDGTSKW SAKITITVVC AQGLQAKDKT GSSDPYVTVQ VGKTKKRTKT
660 670 680 690 700
IFGNLNPVWE EKFHFECHNS SDRIKVRVWD EDDDIKSRVK QRLKRESDDF
710 720 730 740 750
LGQTIIEVRT LSGEMDVWYN LEKRTDKSAV SGAIRLQISV EIKGEEKVAP
760 770 780 790 800
YHVQYTCLHE NLFHYLTDIQ GSGGVWIPEA RGDDAWKVYF DETAQEIVDE
810 820 830 840 850
FAMRYGIESI YQAMTHFACL SSKYMCPGVP AVMSTLLANI NAYYAHTTAS
860 870 880 890 900
TNVSASDRFA ASNFGKERFV KLLDQLHNSL RIDLSTYRNN FPAGSPERLQ
910 920 930 940 950
DLKSTVDLLT SITFFRMKVQ ELQSPPRASQ VVKDCVKACL NSTYEYIFNN
960 970 980 990 1000
CHDLYSHQYQ LQEQPLEEPG PSIRNLDFWP KLITLIVSII EEDKNSYTPV
1010 1020 1030 1040 1050
LSQFPQELNV GKVSAEVMWH LFAQDMKYAL EEHEKDRLCK SADYMNLHFK
1060 1070 1080 1090 1100
VKWLHNEYVR DLPALQGQVP EYPAWFEQFV LQWLDENEDV SLEFLRGALE
1110 1120 1130 1140 1150
RDKKDGFQQT SEHALFSCSV VDVFTQLNQS FEIIRKLECP DPNILAHYMR
1160 1170 1180 1190 1200
RFAKTIGKVL MQYADILSKN FPAYCTKERL PCILMNNMQQ LRVQLEKMFE
1210 1220 1230 1240 1250
AMGGKELDSE AADSLKELQV KLNTVLDELS MVFGNSFQVR IDECVRQMAD
1260 1270 1280 1290 1300
ILGQVRGTGN ASPNARASVA QDADSVLRPL MDFLDGNLTL FATVCEKTVL
1310 1320 1330 1340 1350
KRVLKELWRV VMNTMERVIV LPPLTDQTGT QLILTAAKEL SQLSKLKDHM
1360 1370 1380 1390 1400
VREETRNLTP KQCAVLDLAL DTIKQYFHAG GNGLKKTFLE KSPDLQSLRY
1410 1420 1430 1440 1450
ALSLYTQTTD TLIKTFVRSQ TAQGAGVDDP VGEVSIQVDL FTHPGTGEHK
1460 1470 1480 1490 1500
VTVKVVAAND LKWQTAGMFR PFVEVTMVGP HQSDKKRKFT TKSKSNNWTP
1510 1520 1530 1540 1550
KYNETFHFLL GNEEGPEAYE LQICVKDYCF AREDRVIGLA VMPLRDVAAK
1560 1570 1580 1590 1600
GSCACWCPLG RKIHMDETGM TILRILSQRS NDEVAREFVK LKSESRSTEE

GS
Length:1,602
Mass (Da):181,813
Last modified:May 18, 2010 - v2
Checksum:iFED63BFE566554D2
GO
Isoform 2 (identifier: Q9Z1N9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     176-188: CHWTYLGWGEHQT → S

Show »
Length:1,590
Mass (Da):180,300
Checksum:iD686E2CE66FA2D6B
GO
Isoform 3 (identifier: Q9Z1N9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     962-962: Missing.

Show »
Length:1,601
Mass (Da):181,685
Checksum:iC17F209366F018CE
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A140LHX5A0A140LHX5_MOUSE
Protein unc-13 homolog B
Unc13b
1,982Annotation score:
A0A140LJ69A0A140LJ69_MOUSE
Protein unc-13 homolog B
Unc13b
4,390Annotation score:
E9Q263E9Q263_MOUSE
Protein unc-13 homolog B
Unc13b
1,609Annotation score:
F6X605F6X605_MOUSE
Protein unc-13 homolog B
Unc13b
256Annotation score:
F7CEK4F7CEK4_MOUSE
Protein unc-13 homolog B
Unc13b
189Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31V → E in AAD13619 (Ref. 2) Curated1
Sequence conflicti208S → D in AAD13619 (Ref. 2) Curated1
Sequence conflicti234L → W in BAD32690 (PubMed:15330860).Curated1
Sequence conflicti234L → W in AAD13619 (Ref. 2) Curated1
Sequence conflicti262R → H in AAD13619 (Ref. 2) Curated1
Sequence conflicti353H → Y in BAD32690 (PubMed:15330860).Curated1
Sequence conflicti353H → Y in AAD13619 (Ref. 2) Curated1
Sequence conflicti512 – 513GL → AV in BAD32690 (PubMed:15330860).Curated2
Sequence conflicti512 – 513GL → AV in AAD13619 (Ref. 2) Curated2
Sequence conflicti579 – 580EV → RI in AAD13619 (Ref. 2) Curated2
Sequence conflicti804R → A in AAD13619 (Ref. 2) Curated1
Sequence conflicti837L → M in AAD13619 (Ref. 2) Curated1
Sequence conflicti1073P → PG in AAD13619 (Ref. 2) Curated1
Sequence conflicti1279 – 1280PL → GV in AAD13619 (Ref. 2) Curated2
Sequence conflicti1280L → P in AAI58026 (Ref. 2) Curated1
Sequence conflicti1418R → G in AAD13619 (Ref. 2) Curated1
Sequence conflicti1492K → R in AAD13619 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039197176 – 188CHWTY…GEHQT → S in isoform 2. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_039198962Missing in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB162894 Genomic DNA Translation: BAD32690.1
AF115848 mRNA Translation: AAD13619.1
AL672276, AL732504, AL772176 Genomic DNA Translation: CAM14326.1
AL732504, AL672276, AL772176 Genomic DNA Translation: CAM16530.1
AL772176, AL672276, AL732504 Genomic DNA Translation: CAM22579.1
BC157967 mRNA Translation: AAI57968.1
BC158025 mRNA Translation: AAI58026.1
CCDSiCCDS38738.1 [Q9Z1N9-3]
CCDS51161.1 [Q9Z1N9-1]
CCDS80089.1 [Q9Z1N9-2]
RefSeqiNP_001074882.1, NM_001081413.2 [Q9Z1N9-3]
NP_001297687.1, NM_001310758.1 [Q9Z1N9-2]
NP_067443.2, NM_021468.3 [Q9Z1N9-1]
UniGeneiMm.128892

Genome annotation databases

EnsembliENSMUST00000079978; ENSMUSP00000078894; ENSMUSG00000028456 [Q9Z1N9-2]
ENSMUST00000107952; ENSMUSP00000103586; ENSMUSG00000028456 [Q9Z1N9-1]
ENSMUST00000163653; ENSMUSP00000128608; ENSMUSG00000028456 [Q9Z1N9-3]
GeneIDi22249
KEGGimmu:22249
UCSCiuc008spg.2 mouse [Q9Z1N9-3]
uc012dcy.1 mouse [Q9Z1N9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB162894 Genomic DNA Translation: BAD32690.1
AF115848 mRNA Translation: AAD13619.1
AL672276, AL732504, AL772176 Genomic DNA Translation: CAM14326.1
AL732504, AL672276, AL772176 Genomic DNA Translation: CAM16530.1
AL772176, AL672276, AL732504 Genomic DNA Translation: CAM22579.1
BC157967 mRNA Translation: AAI57968.1
BC158025 mRNA Translation: AAI58026.1
CCDSiCCDS38738.1 [Q9Z1N9-3]
CCDS51161.1 [Q9Z1N9-1]
CCDS80089.1 [Q9Z1N9-2]
RefSeqiNP_001074882.1, NM_001081413.2 [Q9Z1N9-3]
NP_001297687.1, NM_001310758.1 [Q9Z1N9-2]
NP_067443.2, NM_021468.3 [Q9Z1N9-1]
UniGeneiMm.128892

3D structure databases

ProteinModelPortaliQ9Z1N9
SMRiQ9Z1N9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204444, 1 interactor
STRINGi10090.ENSMUSP00000103586

PTM databases

iPTMnetiQ9Z1N9
PhosphoSitePlusiQ9Z1N9

Proteomic databases

PaxDbiQ9Z1N9
PRIDEiQ9Z1N9

Protocols and materials databases

DNASUi22249
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079978; ENSMUSP00000078894; ENSMUSG00000028456 [Q9Z1N9-2]
ENSMUST00000107952; ENSMUSP00000103586; ENSMUSG00000028456 [Q9Z1N9-1]
ENSMUST00000163653; ENSMUSP00000128608; ENSMUSG00000028456 [Q9Z1N9-3]
GeneIDi22249
KEGGimmu:22249
UCSCiuc008spg.2 mouse [Q9Z1N9-3]
uc012dcy.1 mouse [Q9Z1N9-1]

Organism-specific databases

CTDi10497
MGIiMGI:1342278 Unc13b

Phylogenomic databases

eggNOGiKOG1011 Eukaryota
ENOG410XS5D LUCA
GeneTreeiENSGT00920000149033
HOGENOMiHOG000231404
HOVERGENiHBG057340
InParanoidiQ9Z1N9
KOiK15293
OMAiKEKMPCI
PhylomeDBiQ9Z1N9
TreeFamiTF312844

Enzyme and pathway databases

ReactomeiR-MMU-181429 Serotonin Neurotransmitter Release Cycle
R-MMU-181430 Norepinephrine Neurotransmitter Release Cycle
R-MMU-210500 Glutamate Neurotransmitter Release Cycle
R-MMU-212676 Dopamine Neurotransmitter Release Cycle
R-MMU-264642 Acetylcholine Neurotransmitter Release Cycle

Miscellaneous databases

PROiPR:Q9Z1N9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028456 Expressed in 255 organ(s), highest expression level in submandibular gland
CleanExiMM_UNC13A
MM_UNC13B
ExpressionAtlasiQ9Z1N9 baseline and differential
GenevisibleiQ9Z1N9 MM

Family and domain databases

CDDicd00029 C1, 1 hit
cd04027 C2B_Munc13, 1 hit
Gene3Di2.60.40.150, 3 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR010439 CAPS_dom
IPR014770 Munc13_1
IPR014772 Munc13_dom-2
IPR019558 Munc13_subgr_dom-2
IPR002219 PE/DAG-bd
IPR027080 Unc-13
IPR037302 Unc-13_C2B
PANTHERiPTHR10480 PTHR10480, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF00168 C2, 3 hits
PF06292 DUF1041, 1 hit
PF10540 Membr_traf_MHD, 1 hit
PRINTSiPR00360 C2DOMAIN
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00239 C2, 3 hits
SM01145 DUF1041, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits
PS51258 MHD1, 1 hit
PS51259 MHD2, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUN13B_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1N9
Secondary accession number(s): A2AG43
, B2RXT0, B2RXY6, Q6BCX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: May 18, 2010
Last modified: November 7, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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