UniProtKB - Q9Z1L0 (PK3CB_RAT)
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
Pik3cb
Functioni
Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) derivatives at position 3 of the inositol ring to produce 3-phosphoinositides. Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors.
By similarityCatalytic activityi
- a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + ADP + H+By similarityEC:2.7.1.153By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-(1D-myo-inositol 3,4,5-triphosphate) + ADP + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
: phosphatidylinositol phosphate biosynthesis Pathwayi
This protein is involved in the pathway phosphatidylinositol phosphate biosynthesis, which is part of Phospholipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway phosphatidylinositol phosphate biosynthesis and in Phospholipid metabolism.
GO - Molecular functioni
- 1-phosphatidylinositol-3-kinase activity Source: RGD
- 1-phosphatidylinositol-4-phosphate 3-kinase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- insulin receptor substrate binding Source: RGD
- kinase activity Source: RGD
- phosphatidylinositol-3,4-bisphosphate 5-kinase activity Source: UniProtKB-EC
- phosphatidylinositol 3-kinase activity Source: RGD
- phosphatidylinositol-4,5-bisphosphate 3-kinase activity Source: UniProtKB-EC
- phosphatidylinositol kinase activity Source: GO_Central
GO - Biological processi
- angiogenesis involved in wound healing Source: RGD
- autophagy Source: UniProtKB-KW
- cell migration Source: GO_Central
- cellular calcium ion homeostasis Source: RGD
- embryonic cleavage Source: RGD
- endocytosis Source: UniProtKB-KW
- endothelial cell proliferation Source: RGD
- homophilic cell adhesion via plasma membrane adhesion molecules Source: RGD
- negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway Source: RGD
- negative regulation of MAP kinase activity Source: RGD
- negative regulation of protein kinase B signaling Source: RGD
- negative regulation of sprouting angiogenesis Source: RGD
- negative regulation of vascular endothelial growth factor signaling pathway Source: RGD
- phosphatidylinositol 3-kinase signaling Source: RGD
- phosphatidylinositol-3-phosphate biosynthetic process Source: GO_Central
- phosphatidylinositol-mediated signaling Source: GO_Central
- phosphatidylinositol phosphate biosynthetic process Source: RGD
- phosphorylation Source: RGD
- platelet activation Source: RGD
- positive regulation of endothelial cell migration Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of neutrophil apoptotic process Source: RGD
- positive regulation of nitric-oxide synthase activity Source: RGD
- positive regulation of protein kinase B signaling Source: RGD
- positive regulation of Rac protein signal transduction Source: RGD
- regulation of cell-matrix adhesion Source: RGD
- response to ischemia Source: RGD
- response to wounding Source: RGD
- sphingosine-1-phosphate receptor signaling pathway Source: RGD
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Autophagy, Cell adhesion, Endocytosis, Lipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.1.153, 5301 |
Reactomei | R-RNO-109704, PI3K Cascade R-RNO-112399, IRS-mediated signalling R-RNO-114604, GPVI-mediated activation cascade R-RNO-1257604, PIP3 activates AKT signaling R-RNO-1660499, Synthesis of PIPs at the plasma membrane R-RNO-186763, Downstream signal transduction R-RNO-198203, PI3K/AKT activation R-RNO-201556, Signaling by ALK R-RNO-202424, Downstream TCR signaling R-RNO-2029485, Role of phospholipids in phagocytosis R-RNO-210993, Tie2 Signaling R-RNO-2424491, DAP12 signaling R-RNO-2730905, Role of LAT2/NTAL/LAB on calcium mobilization R-RNO-4420097, VEGFA-VEGFR2 Pathway R-RNO-512988, Interleukin-3, Interleukin-5 and GM-CSF signaling R-RNO-5673001, RAF/MAP kinase cascade R-RNO-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-RNO-8853659, RET signaling R-RNO-9027276, Erythropoietin activates Phosphoinositide-3-kinase (PI3K) R-RNO-912526, Interleukin receptor SHC signaling R-RNO-912631, Regulation of signaling by CBL |
UniPathwayi | UPA00220 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform (EC:2.7.1.153By similarity)Short name: PI3-kinase subunit beta Short name: PI3K-beta Short name: PI3Kbeta Short name: PtdIns-3-kinase subunit beta Alternative name(s): Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta Short name: PtdIns-3-kinase subunit p110-beta Short name: p110beta |
Gene namesi | Name:Pik3cb |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620917, Pik3cb |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleus Source: RGD
Plasma Membrane
- brush border membrane Source: RGD
- plasma membrane Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- membrane Source: GO_Central
- phosphatidylinositol 3-kinase complex Source: RGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000088789 | 1 – 1070 | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoformAdd BLAST | 1070 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 324 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1070 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9Z1L0 |
PTM databases
iPTMneti | Q9Z1L0 |
PhosphoSitePlusi | Q9Z1L0 |
Interactioni
Subunit structurei
Heterodimer of a catalytic subunit PIK3CB and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interaction with PIK3R2 is required for nuclear localization and nuclear export (By similarity).
Part of a complex with PIK3R1 and PTEN (By similarity). Binding to PTEN may antagonize the lipid kinase activity under normal growth conditions (By similarity).
Part of a complex involved in autophagosome formation composed of PIK3C3 and PIK3R4 (By similarity).
Interacts with BECN1, ATG14 and RAB5A (By similarity).
By similarityGO - Molecular functioni
- insulin receptor substrate binding Source: RGD
Protein-protein interaction databases
IntActi | Q9Z1L0, 9 interactors |
STRINGi | 10116.ENSRNOP00000022179 |
Chemistry databases
BindingDBi | Q9Z1L0 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 115 | PI3K-ABDPROSITE-ProRule annotationAdd BLAST | 90 | |
Domaini | 194 – 285 | PI3K-RBDPROSITE-ProRule annotationAdd BLAST | 92 | |
Domaini | 327 – 496 | C2 PI3K-typePROSITE-ProRule annotationAdd BLAST | 170 | |
Domaini | 524 – 701 | PIK helicalPROSITE-ProRule annotationAdd BLAST | 178 | |
Domaini | 772 – 1053 | PI3K/PI4K catalyticPROSITE-ProRule annotationAdd BLAST | 282 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 778 – 784 | G-loopPROSITE-ProRule annotation | 7 | |
Regioni | 916 – 924 | Catalytic loopPROSITE-ProRule annotation | 9 | |
Regioni | 935 – 961 | Activation loopPROSITE-ProRule annotationAdd BLAST | 27 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 410 – 418 | Nuclear localization signal (NLS)By similarity | 9 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0904, Eukaryota |
InParanoidi | Q9Z1L0 |
PhylomeDBi | Q9Z1L0 |
Family and domain databases
CDDi | cd05173, PI3Kc_IA_beta, 1 hit |
Gene3Di | 1.10.1070.11, 1 hit 1.25.40.70, 1 hit 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR035892, C2_domain_sf IPR011009, Kinase-like_dom_sf IPR000403, PI3/4_kinase_cat_dom IPR036940, PI3/4_kinase_cat_sf IPR018936, PI3/4_kinase_CS IPR002420, PI3K-type_C2_dom IPR003113, PI3K_ABD IPR001263, PI3K_accessory_dom IPR042236, PI3K_accessory_sf IPR000341, PI3K_Ras-bd_dom IPR037702, PI3Kbeta_dom IPR015433, PI_Kinase IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR10048, PTHR10048, 1 hit |
Pfami | View protein in Pfam PF00454, PI3_PI4_kinase, 1 hit PF00792, PI3K_C2, 1 hit PF02192, PI3K_p85B, 1 hit PF00794, PI3K_rbd, 1 hit PF00613, PI3Ka, 1 hit |
SMARTi | View protein in SMART SM00142, PI3K_C2, 1 hit SM00143, PI3K_p85B, 1 hit SM00144, PI3K_rbd, 1 hit SM00145, PI3Ka, 1 hit SM00146, PI3Kc, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49562, SSF49562, 1 hit SSF54236, SSF54236, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51547, C2_PI3K, 1 hit PS00915, PI3_4_KINASE_1, 1 hit PS00916, PI3_4_KINASE_2, 1 hit PS50290, PI3_4_KINASE_3, 1 hit PS51544, PI3K_ABD, 1 hit PS51546, PI3K_RBD, 1 hit PS51545, PIK_HELICAL, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MCFRSIMPPA MADTLDIWAV DSQIASDGSI SVDFLLPTGI YIQLEVPREA
60 70 80 90 100
TISYIKQMLW KQVHNYPMFN LLMDIDSYMF ACVNQTAVYE ELEDETRRLC
110 120 130 140 150
DVRPFLPVLK LVTRSCDPAE KLDSKIGVLI GKGLHEFDAL KDPEVNEFRR
160 170 180 190 200
KMRKFSEDKI QSLVGLSWID WLKHTYPPEH EPSVLENLED KLYGGKLVVA
210 220 230 240 250
VHFENSQDVF SFQVSPNLNP IKINELAIQK RLTIRGKEEE ASPCDYVLQV
260 270 280 290 300
SGRVEYVFGD HPLIQFQYIR NCVMNRTLPH FILVECCKIK KMYEQEMIAI
310 320 330 340 350
EAAINRNSSS LPLPLPPKKT RVISHVWGNN NPFQIVLVKG NKLNTEETVK
360 370 380 390 400
VHVRAGLFHG TELLCKTVVS SEISGKNDHI WNEQLEFDIN ICDLPRMARL
410 420 430 440 450
CFAVYAVLDK VKTKKSTKTI NPSKYQTIRK AGKVHYPVAW VNTMVFDFKG
460 470 480 490 500
QLRSGDVILH SWSSFPDELE EMLNPMGTVQ TNPYAENATA LHIKFPENKK
510 520 530 540 550
QPYYYPPFDK IIEKAAEIAS GDSANVSSRG GKKFLAVLKE ILDRDPLSQL
560 570 580 590 600
CENEMDLIWT LRQDCRENFP QSLPKLLLSI KWNKLEDVAQ LQALLQIWPK
610 620 630 640 650
LPPREALELL DFNYPDQYVR EYAVGCLRQM SDEELSQYLL QLVQVLKYEP
660 670 680 690 700
FLDCALSRFL LERALDNRRI GQFLFWHLRS EVHTPAVSIQ FGVILEAYCR
710 720 730 740 750
GSVGHMKVLS KQVEALNKLK TLNSLIKLNA MKLNRAKGKE AMHTCLKQSA
760 770 780 790 800
YREALSDLQS PLNPCVILSE LYVEKCRYMD SKMKPLWLVY SNRAFGEDAV
810 820 830 840 850
GVIFKNGDDL RQDMLTLQML RLMDLLWKEA GLDLRMLPYG CLATGDRSGL
860 870 880 890 900
IEVVSTSETI ADIQLNSSNV AATAAFNKDA LLNWLKEYNS GDDLDRAIEE
910 920 930 940 950
FTLSCAGYCV ASYVLGIGDR HSDNIMVKKT GQLFHIDFGH ILGNFKSKFG
960 970 980 990 1000
IKRERVPFIL TYDFIHVIQQ GKTGNTEKFG RFRQCCEDAY LILRRHGNLF
1010 1020 1030 1040 1050
ITLFALMLTA GLPELTSVKD IQYLKDSLAL GKSEEEALKQ FKQKFDEALR
1060 1070
ESWTTKVNWM AHTVRKDYRS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V839 | G3V839_RAT | Phosphatidylinositol-4,5-bisphospha... | Pik3cb rCG_25555 | 1,070 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ012482 mRNA Translation: CAA10046.1 |
RefSeqi | NP_445933.1, NM_053481.2 |
Genome annotation databases
GeneIDi | 85243 |
KEGGi | rno:85243 |
UCSCi | RGD:620917, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ012482 mRNA Translation: CAA10046.1 |
RefSeqi | NP_445933.1, NM_053481.2 |
3D structure databases
SMRi | Q9Z1L0 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q9Z1L0, 9 interactors |
STRINGi | 10116.ENSRNOP00000022179 |
Chemistry databases
BindingDBi | Q9Z1L0 |
ChEMBLi | CHEMBL3608198 |
PTM databases
iPTMneti | Q9Z1L0 |
PhosphoSitePlusi | Q9Z1L0 |
Proteomic databases
PaxDbi | Q9Z1L0 |
Genome annotation databases
GeneIDi | 85243 |
KEGGi | rno:85243 |
UCSCi | RGD:620917, rat |
Organism-specific databases
CTDi | 5291 |
RGDi | 620917, Pik3cb |
Phylogenomic databases
eggNOGi | KOG0904, Eukaryota |
InParanoidi | Q9Z1L0 |
PhylomeDBi | Q9Z1L0 |
Enzyme and pathway databases
UniPathwayi | UPA00220 |
BRENDAi | 2.7.1.153, 5301 |
Reactomei | R-RNO-109704, PI3K Cascade R-RNO-112399, IRS-mediated signalling R-RNO-114604, GPVI-mediated activation cascade R-RNO-1257604, PIP3 activates AKT signaling R-RNO-1660499, Synthesis of PIPs at the plasma membrane R-RNO-186763, Downstream signal transduction R-RNO-198203, PI3K/AKT activation R-RNO-201556, Signaling by ALK R-RNO-202424, Downstream TCR signaling R-RNO-2029485, Role of phospholipids in phagocytosis R-RNO-210993, Tie2 Signaling R-RNO-2424491, DAP12 signaling R-RNO-2730905, Role of LAT2/NTAL/LAB on calcium mobilization R-RNO-4420097, VEGFA-VEGFR2 Pathway R-RNO-512988, Interleukin-3, Interleukin-5 and GM-CSF signaling R-RNO-5673001, RAF/MAP kinase cascade R-RNO-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-RNO-8853659, RET signaling R-RNO-9027276, Erythropoietin activates Phosphoinositide-3-kinase (PI3K) R-RNO-912526, Interleukin receptor SHC signaling R-RNO-912631, Regulation of signaling by CBL |
Miscellaneous databases
PROi | PR:Q9Z1L0 |
Family and domain databases
CDDi | cd05173, PI3Kc_IA_beta, 1 hit |
Gene3Di | 1.10.1070.11, 1 hit 1.25.40.70, 1 hit 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR035892, C2_domain_sf IPR011009, Kinase-like_dom_sf IPR000403, PI3/4_kinase_cat_dom IPR036940, PI3/4_kinase_cat_sf IPR018936, PI3/4_kinase_CS IPR002420, PI3K-type_C2_dom IPR003113, PI3K_ABD IPR001263, PI3K_accessory_dom IPR042236, PI3K_accessory_sf IPR000341, PI3K_Ras-bd_dom IPR037702, PI3Kbeta_dom IPR015433, PI_Kinase IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR10048, PTHR10048, 1 hit |
Pfami | View protein in Pfam PF00454, PI3_PI4_kinase, 1 hit PF00792, PI3K_C2, 1 hit PF02192, PI3K_p85B, 1 hit PF00794, PI3K_rbd, 1 hit PF00613, PI3Ka, 1 hit |
SMARTi | View protein in SMART SM00142, PI3K_C2, 1 hit SM00143, PI3K_p85B, 1 hit SM00144, PI3K_rbd, 1 hit SM00145, PI3Ka, 1 hit SM00146, PI3Kc, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49562, SSF49562, 1 hit SSF54236, SSF54236, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51547, C2_PI3K, 1 hit PS00915, PI3_4_KINASE_1, 1 hit PS00916, PI3_4_KINASE_2, 1 hit PS50290, PI3_4_KINASE_3, 1 hit PS51544, PI3K_ABD, 1 hit PS51546, PI3K_RBD, 1 hit PS51545, PIK_HELICAL, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PK3CB_RAT | |
Accessioni | Q9Z1L0Primary (citable) accession number: Q9Z1L0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | May 1, 1999 | |
Last modified: | February 23, 2022 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families