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Protein

Cysteine desulfurase, mitochondrial

Gene

Nfs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor (By similarity).By similarity

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.

Cofactori

pyridoxal 5'-phosphateBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei209Pyridoxal phosphateBy similarity1
Binding sitei237Pyridoxal phosphateBy similarity1
Binding sitei297Pyridoxal phosphateBy similarity1
Active sitei383Cysteine persulfide intermediateBy similarity1
Metal bindingi383Iron-sulfur (2Fe-2S); via persulfide group; shared with ISCUBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processMolybdenum cofactor biosynthesis
LigandIron, Iron-sulfur, Metal-binding, Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.8.1.7 3474
ReactomeiR-MMU-1362409 Mitochondrial iron-sulfur cluster biogenesis
R-MMU-947581 Molybdenum cofactor biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfurase, mitochondrial (EC:2.8.1.7)
Short name:
m-Nfs1
Gene namesi
Name:Nfs1
Synonyms:Nifs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1316706 Nfs1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 17MitochondrionSequence analysisAdd BLAST17
ChainiPRO_000000129418 – 459Cysteine desulfurase, mitochondrialAdd BLAST442

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei260N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ9Z1J3
PeptideAtlasiQ9Z1J3
PRIDEiQ9Z1J3

2D gel databases

REPRODUCTION-2DPAGEiQ9Z1J3

PTM databases

iPTMnetiQ9Z1J3
PhosphoSitePlusiQ9Z1J3

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSMUSG00000027618 Expressed in 314 organ(s), highest expression level in lobe of liver
CleanExiMM_NFS1
ExpressionAtlasiQ9Z1J3 baseline and differential
GenevisibleiQ9Z1J3 MM

Interactioni

Subunit structurei

Binds ISCU/NIFUN. Forms a complex with LYRM4. Interacts with HSPA9.By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiQ9Z1J3
IntActiQ9Z1J3, 1 interactor
STRINGi10090.ENSMUSP00000029147

Structurei

3D structure databases

ProteinModelPortaliQ9Z1J3
SMRiQ9Z1J3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 130Pyridoxal phosphate bindingBy similarity2
Regioni257 – 259Pyridoxal phosphate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1549 Eukaryota
COG1104 LUCA
GeneTreeiENSGT00530000063513
HOGENOMiHOG000017510
HOVERGENiHBG003708
InParanoidiQ9Z1J3
KOiK04487
OMAiEPIQSGG
OrthoDBiEOG091G072R
PhylomeDBiQ9Z1J3
TreeFamiTF105658

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
HAMAPiMF_00331 Cys_desulf_IscS, 1 hit
InterProiView protein in InterPro
IPR000192 Aminotrans_V_dom
IPR020578 Aminotrans_V_PyrdxlP_BS
IPR010240 Cys_deSase_IscS
IPR016454 Cysteine_dSase
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR11601:SF34 PTHR11601:SF34, 1 hit
PfamiView protein in Pfam
PF00266 Aminotran_5, 1 hit
PIRSFiPIRSF005572 NifS, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR02006 IscS, 1 hit
PROSITEiView protein in PROSITE
PS00595 AA_TRANSFER_CLASS_5, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9Z1J3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVGSVAGNML LRAAWRRASL AATSLALGRS SVPTRGLRLR VVDHGPHSPV
60 70 80 90 100
HSEAEAVLRP LYMDVQATTP LDPRVLDAML PYLVNYYGNP HSRTHAYGWE
110 120 130 140 150
SEAAMERARQ QVASLIGADP REIIFTSGAT ESNNIAIKGV ARFYRSRKKH
160 170 180 190 200
LVTTQTEHKC VLDSCRSLEA EGFRVTYLPV QKSGIIDLKE LEAAIQPDTS
210 220 230 240 250
LVSVMTVNNE IGVKQPIAEI GQICSSRKVY FHTDAAQAVG KIPLDVNDMK
260 270 280 290 300
IDLMSISGHK LYGPKGVGAI YIRRRPRVRV EALQSGGGQE RGMRSGTVPT
310 320 330 340 350
PLVVGLGAAC ELAQQEMEYD HKRISKLAER LVQNIMKNLP DVVMNGDPKQ
360 370 380 390 400
HYPGCINLSF AYVEGESLLM ALKDVALSSG SACTSASLEP SYVLRAIGTD
410 420 430 440 450
EDLAHSSIRF GIGRFTTEEE VDYTAEKCIH HVKRLREMSP LWEMVQDGID

LKSIKWTQH
Length:459
Mass (Da):50,570
Last modified:September 18, 2013 - v3
Checksum:i986D636C5506C7B0
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A6QRH3A6QRH3_MOUSE
Cysteine desulfurase, mitochondrial
Nfs1
148Annotation score:
V9GXS4V9GXS4_MOUSE
Cysteine desulfurase, mitochondrial
Nfs1
141Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti221G → R in CAA10916 (PubMed:9738949).Curated1
Sequence conflicti334N → K in CAA10916 (PubMed:9738949).Curated1
Sequence conflicti394L → F in CAA10916 (PubMed:9738949).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ222660 mRNA Translation: CAA10916.1
AK075575 mRNA Translation: BAC35831.1
AK132838 mRNA Translation: BAE21386.1
AK148527 mRNA Translation: BAE28601.1
AK159828 mRNA Translation: BAE35407.1
AL833786 Genomic DNA No translation available.
BX649640 Genomic DNA No translation available.
CH466551 Genomic DNA Translation: EDL06179.1
CCDSiCCDS16962.1
RefSeqiNP_035041.2, NM_010911.2
UniGeneiMm.283604

Genome annotation databases

EnsembliENSMUST00000029147; ENSMUSP00000029147; ENSMUSG00000027618
GeneIDi18041
KEGGimmu:18041
UCSCiuc008nmj.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ222660 mRNA Translation: CAA10916.1
AK075575 mRNA Translation: BAC35831.1
AK132838 mRNA Translation: BAE21386.1
AK148527 mRNA Translation: BAE28601.1
AK159828 mRNA Translation: BAE35407.1
AL833786 Genomic DNA No translation available.
BX649640 Genomic DNA No translation available.
CH466551 Genomic DNA Translation: EDL06179.1
CCDSiCCDS16962.1
RefSeqiNP_035041.2, NM_010911.2
UniGeneiMm.283604

3D structure databases

ProteinModelPortaliQ9Z1J3
SMRiQ9Z1J3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiQ9Z1J3
IntActiQ9Z1J3, 1 interactor
STRINGi10090.ENSMUSP00000029147

PTM databases

iPTMnetiQ9Z1J3
PhosphoSitePlusiQ9Z1J3

2D gel databases

REPRODUCTION-2DPAGEiQ9Z1J3

Proteomic databases

PaxDbiQ9Z1J3
PeptideAtlasiQ9Z1J3
PRIDEiQ9Z1J3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029147; ENSMUSP00000029147; ENSMUSG00000027618
GeneIDi18041
KEGGimmu:18041
UCSCiuc008nmj.2 mouse

Organism-specific databases

CTDi9054
MGIiMGI:1316706 Nfs1

Phylogenomic databases

eggNOGiKOG1549 Eukaryota
COG1104 LUCA
GeneTreeiENSGT00530000063513
HOGENOMiHOG000017510
HOVERGENiHBG003708
InParanoidiQ9Z1J3
KOiK04487
OMAiEPIQSGG
OrthoDBiEOG091G072R
PhylomeDBiQ9Z1J3
TreeFamiTF105658

Enzyme and pathway databases

BRENDAi2.8.1.7 3474
ReactomeiR-MMU-1362409 Mitochondrial iron-sulfur cluster biogenesis
R-MMU-947581 Molybdenum cofactor biosynthesis

Miscellaneous databases

ChiTaRSiNfs1 mouse
PROiPR:Q9Z1J3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027618 Expressed in 314 organ(s), highest expression level in lobe of liver
CleanExiMM_NFS1
ExpressionAtlasiQ9Z1J3 baseline and differential
GenevisibleiQ9Z1J3 MM

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
HAMAPiMF_00331 Cys_desulf_IscS, 1 hit
InterProiView protein in InterPro
IPR000192 Aminotrans_V_dom
IPR020578 Aminotrans_V_PyrdxlP_BS
IPR010240 Cys_deSase_IscS
IPR016454 Cysteine_dSase
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR11601:SF34 PTHR11601:SF34, 1 hit
PfamiView protein in Pfam
PF00266 Aminotran_5, 1 hit
PIRSFiPIRSF005572 NifS, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR02006 IscS, 1 hit
PROSITEiView protein in PROSITE
PS00595 AA_TRANSFER_CLASS_5, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNFS1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1J3
Secondary accession number(s): Q8C6I5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: September 18, 2013
Last modified: November 7, 2018
This is version 155 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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