UniProtKB - Q9Z1J3 (NFS1_MOUSE)

BLAST

>sp|Q9Z1J3|NFS1_MOUSE Cysteine desulfurase, mitochondrial OS=Mus musculus OX=10090 GN=Nfs1 PE=1 SV=3
MVGSVAGNMLLRAAWRRASLAATSLALGRSSVPTRGLRLRVVDHGPHSPVHSEAEAVLRP
LYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGADP
REIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYLPV
QKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKVYFHTDAAQAVG
KIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTVPT
PLVVGLGAACELAQQEMEYDHKRISKLAERLVQNIMKNLPDVVMNGDPKQHYPGCINLSF
AYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEE
VDYTAEKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH

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History
Entry version 155 (07 Nov 2018)
Sequence version 3 (18 Sep 2013)
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Protein

Cysteine desulfurase, mitochondrial

Gene

Nfs1

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor (By similarity).By similarity

Catalytic activityⁱ

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.

Cofactorⁱ

pyridoxal 5'-phosphateBy similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	209	Pyridoxal phosphateBy similarity	1
Binding siteⁱ	237	Pyridoxal phosphateBy similarity	1
Binding siteⁱ	297	Pyridoxal phosphateBy similarity	1
Active siteⁱ	383	Cysteine persulfide intermediateBy similarity	1
Metal bindingⁱ	383	Iron-sulfur (2Fe-2S); via persulfide group; shared with ISCUBy similarity	1

GO - Molecular functionⁱ

cysteine desulfurase activity Source: MGI
iron-sulfur cluster binding Source: UniProtKB-KW
metal ion binding Source: UniProtKB-KW
protein homodimerization activity Source: MGI
pyridoxal phosphate binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

[2Fe-2S] cluster assembly Source: InterPro
iron incorporation into metallo-sulfur cluster
Source: HGNC
Inferred from direct assayⁱ
- 16847322
Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Molybdenum cofactor biosynthesis
Ligand	Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate

Enzyme and pathway databases

BRENDAⁱ	2.8.1.7 3474
Reactomeⁱ	R-MMU-1362409 Mitochondrial iron-sulfur cluster biogenesis R-MMU-947581 Molybdenum cofactor biosynthesis

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Cysteine desulfurase, mitochondrial (EC:2.8.1.7) Short name: m-Nfs1
Gene namesⁱ	Name:Nfs1 Synonyms:Nifs
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 2

Organism-specific databases

MGIⁱ	MGI:1316706 Nfs1

Keywords - Cellular componentⁱ

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transit peptideⁱ	1 – 17	MitochondrionSequence analysisAdd BLAST		17
ChainⁱPRO_0000001294	18 – 459	Cysteine desulfurase, mitochondrialAdd BLAST		442

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	260	N6-(pyridoxal phosphate)lysineBy similarity		1

Proteomic databases

PaxDbⁱ	Q9Z1J3
PeptideAtlas More...PeptideAtlasⁱ	Q9Z1J3
PRIDEⁱ	Q9Z1J3

2D gel databases

REPRODUCTION-2DPAGE More...REPRODUCTION-2DPAGEⁱ	Q9Z1J3

REPRODUCTION-2DPAGEⁱ

Q9Z1J3

PTM databases

iPTMnetⁱ	Q9Z1J3
PhosphoSitePlusⁱ	Q9Z1J3

Expressionⁱ

Tissue specificityⁱ

Ubiquitous.

Gene expression databases

Bgeeⁱ	ENSMUSG00000027618 Expressed in 314 organ(s), highest expression level in lobe of liver
CleanExⁱ	MM_NFS1
ExpressionAtlasⁱ	Q9Z1J3 baseline and differential
Genevisibleⁱ	Q9Z1J3 MM

Interactionⁱ

Subunit structureⁱ

Binds ISCU/NIFUN. Forms a complex with LYRM4. Interacts with HSPA9.By similarity

GO - Molecular functionⁱ

protein homodimerization activity Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

CORUMⁱ	Q9Z1J3
IntActⁱ	Q9Z1J3, 1 interactor
STRINGⁱ	10090.ENSMUSP00000029147

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9Z1J3
SMRⁱ	Q9Z1J3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	129 – 130	Pyridoxal phosphate bindingBy similarity		2
Regionⁱ	257 – 259	Pyridoxal phosphate bindingBy similarity		3

Sequence similaritiesⁱ

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.Curated

Keywords - Domainⁱ

Transit peptide

Phylogenomic databases

eggNOGⁱ	KOG1549 Eukaryota COG1104 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063513
HOGENOMⁱ	HOG000017510
HOVERGENⁱ	HBG003708
InParanoidⁱ	Q9Z1J3
KEGG Orthology (KO) More...KOⁱ	K04487
OMAⁱ	EPIQSGG
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G072R
PhylomeDBⁱ	Q9Z1J3
TreeFamⁱ	TF105658

Family and domain databases

Gene3Dⁱ	3.40.640.10, 1 hit 3.90.1150.10, 1 hit
HAMAPⁱ	MF_00331 Cys_desulf_IscS, 1 hit
InterProⁱ	View protein in InterPro IPR000192 Aminotrans_V_dom IPR020578 Aminotrans_V_PyrdxlP_BS IPR010240 Cys_deSase_IscS IPR016454 Cysteine_dSase IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major
PANTHERⁱ	PTHR11601:SF34 PTHR11601:SF34, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00266 Aminotran_5, 1 hit
PIRSFⁱ	PIRSF005572 NifS, 1 hit
SUPFAMⁱ	SSF53383 SSF53383, 1 hit
TIGRFAMsⁱ	TIGR02006 IscS, 1 hit
PROSITEⁱ	View protein in PROSITE PS00595 AA_TRANSFER_CLASS_5, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show all Align All

Q9Z1J3-1 [UniParc]FASTA Add to basket

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        10         20         30         40         50
MVGSVAGNML LRAAWRRASL AATSLALGRS SVPTRGLRLR VVDHGPHSPV 
        60         70         80         90        100
HSEAEAVLRP LYMDVQATTP LDPRVLDAML PYLVNYYGNP HSRTHAYGWE 
       110        120        130        140        150
SEAAMERARQ QVASLIGADP REIIFTSGAT ESNNIAIKGV ARFYRSRKKH 
       160        170        180        190        200
LVTTQTEHKC VLDSCRSLEA EGFRVTYLPV QKSGIIDLKE LEAAIQPDTS 
       210        220        230        240        250
LVSVMTVNNE IGVKQPIAEI GQICSSRKVY FHTDAAQAVG KIPLDVNDMK 
       260        270        280        290        300
IDLMSISGHK LYGPKGVGAI YIRRRPRVRV EALQSGGGQE RGMRSGTVPT 
       310        320        330        340        350
PLVVGLGAAC ELAQQEMEYD HKRISKLAER LVQNIMKNLP DVVMNGDPKQ 
       360        370        380        390        400
HYPGCINLSF AYVEGESLLM ALKDVALSSG SACTSASLEP SYVLRAIGTD 
       410        420        430        440        450
EDLAHSSIRF GIGRFTTEEE VDYTAEKCIH HVKRLREMSP LWEMVQDGID 

LKSIKWTQH

Length:459

Mass (Da):50,570

Last modified:September 18, 2013 - v3

Checksum:ⁱ986D636C5506C7B0

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A6QRH3	A6QRH3_MOUSE	Cysteine desulfurase, mitochondrial Cysteine desulfurase, mitochondrial	Nfs1	148	Annotation score:
V9GXS4	V9GXS4_MOUSE	Cysteine desulfurase, mitochondrial Cysteine desulfurase, mitochondrial	Nfs1	141	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	221	G → R in CAA10916 (PubMed:9738949).Curated	1
Sequence conflictⁱ	334	N → K in CAA10916 (PubMed:9738949).Curated	1
Sequence conflictⁱ	394	L → F in CAA10916 (PubMed:9738949).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ222660 mRNA Translation: CAA10916.1 AK075575 mRNA Translation: BAC35831.1 AK132838 mRNA Translation: BAE21386.1 AK148527 mRNA Translation: BAE28601.1 AK159828 mRNA Translation: BAE35407.1 AL833786 Genomic DNA No translation available. BX649640 Genomic DNA No translation available. CH466551 Genomic DNA Translation: EDL06179.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS16962.1
NCBI Reference Sequences More...RefSeqⁱ	NP_035041.2, NM_010911.2
UniGeneⁱ	Mm.283604

Genome annotation databases

Ensemblⁱ	ENSMUST00000029147; ENSMUSP00000029147; ENSMUSG00000027618
GeneIDⁱ	18041
KEGGⁱ	mmu:18041
UCSC genome browser More...UCSCⁱ	uc008nmj.2 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9Z1J3	Uncharacterized protein		MAIZE		459	UniRef90_Q9Z1J3
Cysteine desulfurase, mitochondrial		RAT		459
Nitrogen fixation gene 1 (S. cerevisiae)		MOUSE		459
Cysteine desulfurase, mitochondrial		HETGA		457
UPI000E560442		UROPR		466
+6

Protein	Similar proteins		Species	Score	Length	Source
Q9Z1J3	Nitrogen fixation gene 1 (S. cerevisiae)		MOUSE		459	UniRef50_Q9Z1J3
Uncharacterized protein		MAIZE		459
Cysteine desulfurase, mitochondrial		RAT		459
Cysteine desulfurase, mitochondrial		HETGA		457
CG12264		DROBS		459
+176

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ222660 mRNA Translation: CAA10916.1 AK075575 mRNA Translation: BAC35831.1 AK132838 mRNA Translation: BAE21386.1 AK148527 mRNA Translation: BAE28601.1 AK159828 mRNA Translation: BAE35407.1 AL833786 Genomic DNA No translation available. BX649640 Genomic DNA No translation available. CH466551 Genomic DNA Translation: EDL06179.1
CCDSⁱ	CCDS16962.1
RefSeqⁱ	NP_035041.2, NM_010911.2
UniGeneⁱ	Mm.283604

3D structure databases

ProteinModelPortalⁱ	Q9Z1J3
SMRⁱ	Q9Z1J3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

CORUMⁱ	Q9Z1J3
IntActⁱ	Q9Z1J3, 1 interactor
STRINGⁱ	10090.ENSMUSP00000029147

PTM databases

iPTMnetⁱ	Q9Z1J3
PhosphoSitePlusⁱ	Q9Z1J3

2D gel databases

REPRODUCTION-2DPAGEⁱ	Q9Z1J3

REPRODUCTION-2DPAGEⁱ

Q9Z1J3

Proteomic databases

PaxDbⁱ	Q9Z1J3
PeptideAtlasⁱ	Q9Z1J3
PRIDEⁱ	Q9Z1J3

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000029147; ENSMUSP00000029147; ENSMUSG00000027618
GeneIDⁱ	18041
KEGGⁱ	mmu:18041
UCSCⁱ	uc008nmj.2 mouse

Organism-specific databases

CTDⁱ	9054
MGIⁱ	MGI:1316706 Nfs1

Phylogenomic databases

eggNOGⁱ	KOG1549 Eukaryota COG1104 LUCA
GeneTreeⁱ	ENSGT00530000063513
HOGENOMⁱ	HOG000017510
HOVERGENⁱ	HBG003708
InParanoidⁱ	Q9Z1J3
KOⁱ	K04487
OMAⁱ	EPIQSGG
OrthoDBⁱ	EOG091G072R
PhylomeDBⁱ	Q9Z1J3
TreeFamⁱ	TF105658

Enzyme and pathway databases

BRENDAⁱ	2.8.1.7 3474
Reactomeⁱ	R-MMU-1362409 Mitochondrial iron-sulfur cluster biogenesis R-MMU-947581 Molybdenum cofactor biosynthesis

Miscellaneous databases

ChiTaRSⁱ	Nfs1 mouse
Protein Ontology More...PROⁱ	PR:Q9Z1J3
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000027618 Expressed in 314 organ(s), highest expression level in lobe of liver
CleanExⁱ	MM_NFS1
ExpressionAtlasⁱ	Q9Z1J3 baseline and differential
Genevisibleⁱ	Q9Z1J3 MM

Family and domain databases

Gene3Dⁱ	3.40.640.10, 1 hit 3.90.1150.10, 1 hit
HAMAPⁱ	MF_00331 Cys_desulf_IscS, 1 hit
InterProⁱ	View protein in InterPro IPR000192 Aminotrans_V_dom IPR020578 Aminotrans_V_PyrdxlP_BS IPR010240 Cys_deSase_IscS IPR016454 Cysteine_dSase IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major
PANTHERⁱ	PTHR11601:SF34 PTHR11601:SF34, 1 hit
Pfamⁱ	View protein in Pfam PF00266 Aminotran_5, 1 hit
PIRSFⁱ	PIRSF005572 NifS, 1 hit
SUPFAMⁱ	SSF53383 SSF53383, 1 hit
TIGRFAMsⁱ	TIGR02006 IscS, 1 hit
PROSITEⁱ	View protein in PROSITE PS00595 AA_TRANSFER_CLASS_5, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NFS1_MOUSE
Accessionⁱ	Q9Z1J3Primary (citable) accession number: Q9Z1J3 Secondary accession number(s): Q8C6I5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
	Last sequence update:	September 18, 2013
	Last modified:	November 7, 2018
	This is version 155 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9Z1J3 (NFS1_MOUSE)

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Cysteine desulfurase, mitochondrial

Nfs1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Nucleus

Other locations

Cytosol

Mitochondrion

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ