UniProtKB - Q9Z1J3 (NFS1_MOUSE)
Protein
Cysteine desulfurase, mitochondrial
Gene
Nfs1
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor (By similarity).By similarity
Catalytic activityi
- EC:2.8.1.7
Cofactori
pyridoxal 5'-phosphateBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 209 | Pyridoxal phosphateBy similarity | 1 | |
Binding sitei | 237 | Pyridoxal phosphateBy similarity | 1 | |
Binding sitei | 297 | Pyridoxal phosphateBy similarity | 1 | |
Active sitei | 383 | Cysteine persulfide intermediateBy similarity | 1 | |
Metal bindingi | 383 | Iron-sulfur (2Fe-2S); via persulfide group; shared with ISCUBy similarity | 1 |
GO - Molecular functioni
- cysteine desulfurase activity Source: MGI
- iron-sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- protein homodimerization activity Source: MGI
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- [2Fe-2S] cluster assembly Source: InterPro
- iron incorporation into metallo-sulfur cluster Source: HGNC-UCL
- iron-sulfur cluster assembly Source: GO_Central
- Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Molybdenum cofactor biosynthesis |
Ligand | Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate |
Enzyme and pathway databases
BRENDAi | 2.8.1.7, 3474 |
Reactomei | R-MMU-1362409, Mitochondrial iron-sulfur cluster biogenesis R-MMU-947581, Molybdenum cofactor biosynthesis |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Nfs1 Synonyms:Nifs |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1316706, Nfs1 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Nucleus
- Nucleus By similarity
Other locations
- Cytoplasm By similarity
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrial matrix Source: MGI
- mitochondrion Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: HGNC-UCL
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 17 | MitochondrionSequence analysisAdd BLAST | 17 | |
ChainiPRO_0000001294 | 18 – 459 | Cysteine desulfurase, mitochondrialAdd BLAST | 442 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 260 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Proteomic databases
jPOSTi | Q9Z1J3 |
PaxDbi | Q9Z1J3 |
PeptideAtlasi | Q9Z1J3 |
PRIDEi | Q9Z1J3 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9Z1J3 |
PTM databases
iPTMneti | Q9Z1J3 |
PhosphoSitePlusi | Q9Z1J3 |
Expressioni
Tissue specificityi
Ubiquitous.
Gene expression databases
Bgeei | ENSMUSG00000027618, Expressed in lobe of liver and 330 other tissues |
ExpressionAtlasi | Q9Z1J3, baseline and differential |
Genevisiblei | Q9Z1J3, MM |
Interactioni
Subunit structurei
Binds ISCU/NIFUN.
Forms a complex with LYRM4.
Interacts with HSPA9.
By similarityGO - Molecular functioni
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 201759, 2 interactors |
ComplexPortali | CPX-5823, Mitochondrial iron-sulfur cluster assembly complex |
CORUMi | Q9Z1J3 |
IntActi | Q9Z1J3, 1 interactor |
STRINGi | 10090.ENSMUSP00000029147 |
Miscellaneous databases
RNActi | Q9Z1J3, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 129 – 130 | Pyridoxal phosphate bindingBy similarity | 2 | |
Regioni | 257 – 259 | Pyridoxal phosphate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1549, Eukaryota |
GeneTreei | ENSGT00940000155740 |
HOGENOMi | CLU_003433_0_2_1 |
InParanoidi | Q9Z1J3 |
OMAi | AVKGLYW |
OrthoDBi | 664730at2759 |
PhylomeDBi | Q9Z1J3 |
TreeFami | TF105658 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_00331, Cys_desulf_IscS, 1 hit |
InterProi | View protein in InterPro IPR000192, Aminotrans_V_dom IPR020578, Aminotrans_V_PyrdxlP_BS IPR010240, Cys_deSase_IscS IPR016454, Cysteine_dSase IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11601:SF34, PTHR11601:SF34, 1 hit |
Pfami | View protein in Pfam PF00266, Aminotran_5, 1 hit |
PIRSFi | PIRSF005572, NifS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR02006, IscS, 1 hit |
PROSITEi | View protein in PROSITE PS00595, AA_TRANSFER_CLASS_5, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9Z1J3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVGSVAGNML LRAAWRRASL AATSLALGRS SVPTRGLRLR VVDHGPHSPV
60 70 80 90 100
HSEAEAVLRP LYMDVQATTP LDPRVLDAML PYLVNYYGNP HSRTHAYGWE
110 120 130 140 150
SEAAMERARQ QVASLIGADP REIIFTSGAT ESNNIAIKGV ARFYRSRKKH
160 170 180 190 200
LVTTQTEHKC VLDSCRSLEA EGFRVTYLPV QKSGIIDLKE LEAAIQPDTS
210 220 230 240 250
LVSVMTVNNE IGVKQPIAEI GQICSSRKVY FHTDAAQAVG KIPLDVNDMK
260 270 280 290 300
IDLMSISGHK LYGPKGVGAI YIRRRPRVRV EALQSGGGQE RGMRSGTVPT
310 320 330 340 350
PLVVGLGAAC ELAQQEMEYD HKRISKLAER LVQNIMKNLP DVVMNGDPKQ
360 370 380 390 400
HYPGCINLSF AYVEGESLLM ALKDVALSSG SACTSASLEP SYVLRAIGTD
410 420 430 440 450
EDLAHSSIRF GIGRFTTEEE VDYTAEKCIH HVKRLREMSP LWEMVQDGID
LKSIKWTQH
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketV9GXS4 | V9GXS4_MOUSE | Cysteine desulfurase, mitochondrial | Nfs1 | 141 | Annotation score: | ||
A6QRH3 | A6QRH3_MOUSE | Cysteine desulfurase, mitochondrial | Nfs1 | 148 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 221 | G → R in CAA10916 (PubMed:9738949).Curated | 1 | |
Sequence conflicti | 334 | N → K in CAA10916 (PubMed:9738949).Curated | 1 | |
Sequence conflicti | 394 | L → F in CAA10916 (PubMed:9738949).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ222660 mRNA Translation: CAA10916.1 AK075575 mRNA Translation: BAC35831.1 AK132838 mRNA Translation: BAE21386.1 AK148527 mRNA Translation: BAE28601.1 AK159828 mRNA Translation: BAE35407.1 AL833786 Genomic DNA No translation available. BX649640 Genomic DNA No translation available. CH466551 Genomic DNA Translation: EDL06179.1 |
CCDSi | CCDS16962.1 |
RefSeqi | NP_035041.2, NM_010911.2 |
Genome annotation databases
Ensembli | ENSMUST00000029147; ENSMUSP00000029147; ENSMUSG00000027618 |
GeneIDi | 18041 |
KEGGi | mmu:18041 |
UCSCi | uc008nmj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ222660 mRNA Translation: CAA10916.1 AK075575 mRNA Translation: BAC35831.1 AK132838 mRNA Translation: BAE21386.1 AK148527 mRNA Translation: BAE28601.1 AK159828 mRNA Translation: BAE35407.1 AL833786 Genomic DNA No translation available. BX649640 Genomic DNA No translation available. CH466551 Genomic DNA Translation: EDL06179.1 |
CCDSi | CCDS16962.1 |
RefSeqi | NP_035041.2, NM_010911.2 |
3D structure databases
SMRi | Q9Z1J3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201759, 2 interactors |
ComplexPortali | CPX-5823, Mitochondrial iron-sulfur cluster assembly complex |
CORUMi | Q9Z1J3 |
IntActi | Q9Z1J3, 1 interactor |
STRINGi | 10090.ENSMUSP00000029147 |
PTM databases
iPTMneti | Q9Z1J3 |
PhosphoSitePlusi | Q9Z1J3 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9Z1J3 |
Proteomic databases
jPOSTi | Q9Z1J3 |
PaxDbi | Q9Z1J3 |
PeptideAtlasi | Q9Z1J3 |
PRIDEi | Q9Z1J3 |
Genome annotation databases
Ensembli | ENSMUST00000029147; ENSMUSP00000029147; ENSMUSG00000027618 |
GeneIDi | 18041 |
KEGGi | mmu:18041 |
UCSCi | uc008nmj.2, mouse |
Organism-specific databases
CTDi | 9054 |
MGIi | MGI:1316706, Nfs1 |
Phylogenomic databases
eggNOGi | KOG1549, Eukaryota |
GeneTreei | ENSGT00940000155740 |
HOGENOMi | CLU_003433_0_2_1 |
InParanoidi | Q9Z1J3 |
OMAi | AVKGLYW |
OrthoDBi | 664730at2759 |
PhylomeDBi | Q9Z1J3 |
TreeFami | TF105658 |
Enzyme and pathway databases
BRENDAi | 2.8.1.7, 3474 |
Reactomei | R-MMU-1362409, Mitochondrial iron-sulfur cluster biogenesis R-MMU-947581, Molybdenum cofactor biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 18041, 10 hits in 20 CRISPR screens |
ChiTaRSi | Nfs1, mouse |
PROi | PR:Q9Z1J3 |
RNActi | Q9Z1J3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027618, Expressed in lobe of liver and 330 other tissues |
ExpressionAtlasi | Q9Z1J3, baseline and differential |
Genevisiblei | Q9Z1J3, MM |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_00331, Cys_desulf_IscS, 1 hit |
InterProi | View protein in InterPro IPR000192, Aminotrans_V_dom IPR020578, Aminotrans_V_PyrdxlP_BS IPR010240, Cys_deSase_IscS IPR016454, Cysteine_dSase IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11601:SF34, PTHR11601:SF34, 1 hit |
Pfami | View protein in Pfam PF00266, Aminotran_5, 1 hit |
PIRSFi | PIRSF005572, NifS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR02006, IscS, 1 hit |
PROSITEi | View protein in PROSITE PS00595, AA_TRANSFER_CLASS_5, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NFS1_MOUSE | |
Accessioni | Q9Z1J3Primary (citable) accession number: Q9Z1J3 Secondary accession number(s): Q8C6I5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | September 18, 2013 | |
Last modified: | February 10, 2021 | |
This is version 168 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families