UniProtKB - Q9Z1F9 (SAE2_MOUSE)
Protein
SUMO-activating enzyme subunit 2
Gene
Uba2
Organism
Mus musculus (Mouse)
Status
Functioni
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 (By similarity).By similarity
: protein sumoylation Pathwayi
This protein is involved in the pathway protein sumoylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 48 | ATPBy similarity | 1 | |
Binding sitei | 72 | ATPBy similarity | 1 | |
Metal bindingi | 158 | ZincBy similarity | 1 | |
Metal bindingi | 161 | ZincBy similarity | 1 | |
Active sitei | 173 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 | |
Metal bindingi | 439 | ZincBy similarity | 1 | |
Metal bindingi | 442 | ZincBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 24 – 29 | ATPBy similarity | 6 | |
Nucleotide bindingi | 56 – 59 | ATPBy similarity | 4 | |
Nucleotide bindingi | 95 – 96 | ATPBy similarity | 2 | |
Nucleotide bindingi | 117 – 122 | ATPBy similarity | 6 |
GO - Molecular functioni
- ATP binding Source: MGI
- magnesium ion binding Source: MGI
- protein heterodimerization activity Source: MGI
- small protein activating enzyme binding Source: MGI
- SUMO activating enzyme activity Source: MGI
- SUMO binding Source: MGI
- transcription factor binding Source: MGI
- transferase activity Source: UniProtKB-KW
- ubiquitin-like protein conjugating enzyme binding Source: MGI
GO - Biological processi
- positive regulation of protein sumoylation Source: MGI
- protein modification by small protein conjugation Source: GO_Central
- protein sumoylation Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-3065676, SUMO is conjugated to E1 (UBA2:SAE1) R-MMU-3065678, SUMO is transferred from E1 to E2 (UBE2I, UBC9) |
UniPathwayi | UPA00886 |
Names & Taxonomyi
Protein namesi | Recommended name: SUMO-activating enzyme subunit 2 (EC:2.3.2.-)Alternative name(s): Anthracycline-associated resistance ARX Ubiquitin-like 1-activating enzyme E1B Ubiquitin-like modifier-activating enzyme 2 |
Gene namesi | Name:Uba2 Synonyms:Sae2, Uble1b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1858313, Uba2 |
Subcellular locationi
Nucleus
- nucleoplasm Source: MGI
- SUMO activating enzyme complex Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194969 | 1 – 638 | SUMO-activating enzyme subunit 2Add BLAST | 638 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 164 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity | ||
Cross-linki | 190 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 207 | PhosphoserineCombined sources | 1 | |
Cross-linki | 236 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 236 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 257 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity | ||
Cross-linki | 257 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 271 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 271 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity | ||
Cross-linki | 275 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Cross-linki | 369 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 418 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 418 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 505 | PhosphoserineBy similarity | 1 | |
Cross-linki | 538 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 548 | PhosphoserineCombined sources | 1 | |
Modified residuei | 590 | PhosphoserineCombined sources | 1 | |
Cross-linki | 609 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 611 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 611 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity | ||
Cross-linki | 621 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity |
Post-translational modificationi
Sumoylated with SUMO1 and SUMO2/3 and by UBC9. Sumoylation at Lys-236 inhibits enzymatic activity. Sumoylation at the C-terminal lysine cluster plays an essential role in nuclear trafficking (By similarity).By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9Z1F9 |
jPOSTi | Q9Z1F9 |
MaxQBi | Q9Z1F9 |
PaxDbi | Q9Z1F9 |
PeptideAtlasi | Q9Z1F9 |
PRIDEi | Q9Z1F9 |
PTM databases
iPTMneti | Q9Z1F9 |
PhosphoSitePlusi | Q9Z1F9 |
SwissPalmi | Q9Z1F9 |
Expressioni
Tissue specificityi
Broadly expressed, with highest levels in testis.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000052997, Expressed in ear vesicle and 312 other tissues |
Genevisiblei | Q9Z1F9, MM |
Interactioni
Subunit structurei
Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1.
Interacts with UBE2I (By similarity).
By similarityGO - Molecular functioni
- protein heterodimerization activity Source: MGI
- small protein activating enzyme binding Source: MGI
- SUMO binding Source: MGI
- transcription factor binding Source: MGI
- ubiquitin-like protein conjugating enzyme binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 206169, 21 interactors |
ComplexPortali | CPX-3041, SUMO activating enzyme complex CPX-3046, activated SUMO1-E1 ligase complex CPX-3047, activated SUMO2-E1 ligase complex CPX-3048, activated SUMO3-E1 ligase complex |
IntActi | Q9Z1F9, 3 interactors |
MINTi | Q9Z1F9 |
STRINGi | 10090.ENSMUSP00000099807 |
Miscellaneous databases
RNActi | Q9Z1F9, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ubiquitin-activating E1 family.Curated
Phylogenomic databases
eggNOGi | KOG2013, Eukaryota |
GeneTreei | ENSGT00550000074924 |
HOGENOMi | CLU_013325_7_4_1 |
InParanoidi | Q9Z1F9 |
OMAi | YECTPKA |
OrthoDBi | 686413at2759 |
PhylomeDBi | Q9Z1F9 |
TreeFami | TF300765 |
Family and domain databases
Gene3Di | 1.10.10.520, 1 hit |
InterProi | View protein in InterPro IPR000594, ThiF_NAD_FAD-bd IPR028077, UAE_UbL_dom IPR023318, Ub_act_enz_dom_a_sf IPR030661, Uba2 IPR032426, UBA2_C IPR035985, Ubiquitin-activating_enz IPR018074, UBQ-activ_enz_E1_CS IPR033127, UBQ-activ_enz_E1_Cys_AS |
PANTHERi | PTHR10953:SF5, PTHR10953:SF5, 1 hit |
Pfami | View protein in Pfam PF00899, ThiF, 1 hit PF14732, UAE_UbL, 1 hit PF16195, UBA2_C, 1 hit |
PIRSFi | PIRSF039133, SUMO_E1B, 1 hit |
SUPFAMi | SSF69572, SSF69572, 1 hit |
PROSITEi | View protein in PROSITE PS00536, UBIQUITIN_ACTIVAT_1, 1 hit PS00865, UBIQUITIN_ACTIVAT_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q9Z1F9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALSRGLPRE LAEAVSGGRV LVVGAGGIGC ELLKNLVLTG FSHIDLIDLD
60 70 80 90 100
TIDVSNLNRQ FLFQKKHVGR SKAQVAKESV LQFHPQANIE AHHDSIMNPD
110 120 130 140 150
YNVEFFRQFI LVMNALDNRA ARNHVNRMCL AADVPLIESG TAGYLGQVTT
160 170 180 190 200
IKKGVTECYE CHPKPTQRTF PGCTIRNTPS EPIHCIVWAK YLFNQLFGEE
210 220 230 240 250
DADQEVSPDR ADPEAAWEPT EAEARARASN EDGDIKRIST KEWAKSTGYD
260 270 280 290 300
PVKLFTKLFK DDIRYLLTMD KLWRKRKPPV PLDWAEVQSQ GEANADQQNE
310 320 330 340 350
PQLGLKDQQV LDVKSYASLF SKSIETLRVH LAEKGDGAEL IWDKDDPPAM
360 370 380 390 400
DFVTSAANLR MHIFSMNMKS RFDIKSMAGN IIPAIATTNA VIAGLIVLEG
410 420 430 440 450
LKILSGKIDQ CRTIFLNKQP NPRKKLLVPC ALDPPNTNCY VCASKPEVTV
460 470 480 490 500
RLNVHKVTVL TLQDKIVKEK FAMVAPDVQI EDGKGTILIS SEEGETEANN
510 520 530 540 550
PKKLSDFGIR NGSRLQADDF LQDYTLLINI LHSEDLGKDV EFEVVGDSPE
560 570 580 590 600
KVGPKQAEDA AKSIANGSDD GAQPSTSTAQ EQDDVLIVDS DEEGPSNSTD
610 620 630
CSGDDKARKR KLEENEAAST KKCRLEQMED PDDVIALD
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BLR3 | H3BLR3_MOUSE | SUMO-activating enzyme subunit 2 | Uba2 | 148 | Annotation score: | ||
H3BLM2 | H3BLM2_MOUSE | SUMO-activating enzyme subunit 2 | Uba2 | 132 | Annotation score: | ||
H3BJQ2 | H3BJQ2_MOUSE | SUMO-activating enzyme subunit 2 | Uba2 | 69 | Annotation score: | ||
A2BH29 | A2BH29_MOUSE | SUMO-activating enzyme subunit 2 | Uba2 | 119 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 209 | D → V in BAE37349 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 542 | F → L in BAE30671 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35833 mRNA Translation: AAD10338.1 AK075938 mRNA Translation: BAC36068.1 AK146925 mRNA Translation: BAE27536.1 AK151765 mRNA Translation: BAE30671.1 AK152415 mRNA Translation: BAE31200.1 AK163451 mRNA Translation: BAE37349.1 AK164826 mRNA Translation: BAE37935.1 AK166133 mRNA Translation: BAE38590.1 AK168673 mRNA Translation: BAE40523.1 AK169168 mRNA Translation: BAE40947.1 BC054768 mRNA Translation: AAH54768.1 |
CCDSi | CCDS21136.1 |
RefSeqi | NP_057891.1, NM_016682.2 |
Genome annotation databases
Ensembli | ENSMUST00000102746; ENSMUSP00000099807; ENSMUSG00000052997 |
GeneIDi | 50995 |
KEGGi | mmu:50995 |
UCSCi | uc009giu.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35833 mRNA Translation: AAD10338.1 AK075938 mRNA Translation: BAC36068.1 AK146925 mRNA Translation: BAE27536.1 AK151765 mRNA Translation: BAE30671.1 AK152415 mRNA Translation: BAE31200.1 AK163451 mRNA Translation: BAE37349.1 AK164826 mRNA Translation: BAE37935.1 AK166133 mRNA Translation: BAE38590.1 AK168673 mRNA Translation: BAE40523.1 AK169168 mRNA Translation: BAE40947.1 BC054768 mRNA Translation: AAH54768.1 |
CCDSi | CCDS21136.1 |
RefSeqi | NP_057891.1, NM_016682.2 |
3D structure databases
SMRi | Q9Z1F9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 206169, 21 interactors |
ComplexPortali | CPX-3041, SUMO activating enzyme complex CPX-3046, activated SUMO1-E1 ligase complex CPX-3047, activated SUMO2-E1 ligase complex CPX-3048, activated SUMO3-E1 ligase complex |
IntActi | Q9Z1F9, 3 interactors |
MINTi | Q9Z1F9 |
STRINGi | 10090.ENSMUSP00000099807 |
PTM databases
iPTMneti | Q9Z1F9 |
PhosphoSitePlusi | Q9Z1F9 |
SwissPalmi | Q9Z1F9 |
Proteomic databases
EPDi | Q9Z1F9 |
jPOSTi | Q9Z1F9 |
MaxQBi | Q9Z1F9 |
PaxDbi | Q9Z1F9 |
PeptideAtlasi | Q9Z1F9 |
PRIDEi | Q9Z1F9 |
Protocols and materials databases
Antibodypediai | 29159, 349 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000102746; ENSMUSP00000099807; ENSMUSG00000052997 |
GeneIDi | 50995 |
KEGGi | mmu:50995 |
UCSCi | uc009giu.1, mouse |
Organism-specific databases
CTDi | 10054 |
MGIi | MGI:1858313, Uba2 |
Phylogenomic databases
eggNOGi | KOG2013, Eukaryota |
GeneTreei | ENSGT00550000074924 |
HOGENOMi | CLU_013325_7_4_1 |
InParanoidi | Q9Z1F9 |
OMAi | YECTPKA |
OrthoDBi | 686413at2759 |
PhylomeDBi | Q9Z1F9 |
TreeFami | TF300765 |
Enzyme and pathway databases
UniPathwayi | UPA00886 |
Reactomei | R-MMU-3065676, SUMO is conjugated to E1 (UBA2:SAE1) R-MMU-3065678, SUMO is transferred from E1 to E2 (UBE2I, UBC9) |
Miscellaneous databases
BioGRID-ORCSi | 50995, 11 hits in 17 CRISPR screens |
ChiTaRSi | Uba2, mouse |
PROi | PR:Q9Z1F9 |
RNActi | Q9Z1F9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000052997, Expressed in ear vesicle and 312 other tissues |
Genevisiblei | Q9Z1F9, MM |
Family and domain databases
Gene3Di | 1.10.10.520, 1 hit |
InterProi | View protein in InterPro IPR000594, ThiF_NAD_FAD-bd IPR028077, UAE_UbL_dom IPR023318, Ub_act_enz_dom_a_sf IPR030661, Uba2 IPR032426, UBA2_C IPR035985, Ubiquitin-activating_enz IPR018074, UBQ-activ_enz_E1_CS IPR033127, UBQ-activ_enz_E1_Cys_AS |
PANTHERi | PTHR10953:SF5, PTHR10953:SF5, 1 hit |
Pfami | View protein in Pfam PF00899, ThiF, 1 hit PF14732, UAE_UbL, 1 hit PF16195, UBA2_C, 1 hit |
PIRSFi | PIRSF039133, SUMO_E1B, 1 hit |
SUPFAMi | SSF69572, SSF69572, 1 hit |
PROSITEi | View protein in PROSITE PS00536, UBIQUITIN_ACTIVAT_1, 1 hit PS00865, UBIQUITIN_ACTIVAT_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SAE2_MOUSE | |
Accessioni | Q9Z1F9Primary (citable) accession number: Q9Z1F9 Secondary accession number(s): Q3TQN3 Q8BVX9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 16, 2004 |
Last sequence update: | May 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families