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Protein

Eukaryotic translation initiation factor 3 subunit G

Gene

Eif3g

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit can bind 18S rRNA.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

  • translational initiation Source: UniProtKB
  • viral translational termination-reinitiation Source: MGI

Keywordsi

Molecular functionInitiation factor, RNA-binding
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-MMU-72649 Translation initiation complex formation
R-MMU-72689 Formation of a pool of free 40S subunits
R-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit GUniRule annotation
Short name:
eIF3gUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 RNA-binding subunitUniRule annotation
Short name:
eIF-3 RNA-binding subunitUniRule annotation
Eukaryotic translation initiation factor 3 subunit 4UniRule annotation
eIF-3-deltaUniRule annotation
eIF3 p42UniRule annotation
eIF3 p44UniRule annotation
Gene namesi
Name:Eif3g
Synonyms:Eif3p42, Eif3s4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1858258 Eif3g

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001235111 – 320Eukaryotic translation initiation factor 3 subunit GAdd BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineBy similarity1
Modified residuei11PhosphoserineBy similarity1
Modified residuei38PhosphothreonineUniRule annotationBy similarity1
Modified residuei41PhosphothreonineCombined sources1
Modified residuei42PhosphoserineCombined sources1
Modified residuei189PhosphoserineBy similarity1
Modified residuei223PhosphoserineBy similarity1
Modified residuei264PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Z1D1
MaxQBiQ9Z1D1
PaxDbiQ9Z1D1
PRIDEiQ9Z1D1

2D gel databases

REPRODUCTION-2DPAGEiQ9Z1D1

PTM databases

iPTMnetiQ9Z1D1
PhosphoSitePlusiQ9Z1D1
SwissPalmiQ9Z1D1

Expressioni

Gene expression databases

BgeeiENSMUSG00000070319
ExpressionAtlasiQ9Z1D1 baseline and differential
GenevisibleiQ9Z1D1 MM

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex may interact with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation may lead to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts (via C-terminus) with AIFM1 (via N-terminus) (By similarity). Interacts with DHX33; the interaction is independent of RNA (PubMed:26100019).UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi207295, 5 interactors
IntActiQ9Z1D1, 7 interactors
MINTiQ9Z1D1
STRINGi10090.ENSMUSP00000004206

Structurei

3D structure databases

ProteinModelPortaliQ9Z1D1
SMRiQ9Z1D1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini239 – 317RRMUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the eIF-3 subunit G family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0122 Eukaryota
ENOG410Y3CW LUCA
GeneTreeiENSGT00510000047802
HOGENOMiHOG000239560
HOVERGENiHBG026850
InParanoidiQ9Z1D1
KOiK03248
OMAiKMDGFGF
OrthoDBiEOG091G0GSV
PhylomeDBiQ9Z1D1
TreeFamiTF101516

Family and domain databases

CDDicd12933 eIF3G, 1 hit
cd12408 RRM_eIF3G_like, 1 hit
Gene3Di3.30.70.330, 1 hit
HAMAPiMF_03006 eIF3g, 1 hit
InterProiView protein in InterPro
IPR017334 eIF3_g
IPR024675 eIF3g_N
IPR034240 eIF3G_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR10352:SF0 PTHR10352:SF0, 1 hit
PfamiView protein in Pfam
PF12353 eIF3g, 1 hit
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z1D1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTGDFDSKP SWADQVEEEG EDDKCVTSEL LKGIPLPTGD TSPEPELLPG
60 70 80 90 100
DPLPPPKEVI NGNIKTVTEY KIEEDGKKFK IVRTFRIETR KASKAVARRK
110 120 130 140 150
NWKKFGNSEF DPPGPNVATT TVSDDVSMTF ITSKEDLNCQ EEEDPMNKLK
160 170 180 190 200
GQKIVSCRIC KGDHWTTRCP YKDTLGPMQK ELAEQLGLST GEKEKLPGEL
210 220 230 240 250
EPVQAAQSKT GKYVPPSLRD GASRRGESMQ PNRRADDNAT IRVTNLSEDT
260 270 280 290 300
RETDLQELFR PFGSISRIYL AKDKTTGQSK GFAFISFHRR EDAARAIAGV
310 320
SGFGYDHLIL NVEWAKPSTN
Length:320
Mass (Da):35,638
Last modified:September 19, 2002 - v2
Checksum:i1B46670351E489AC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17 – 18EE → AA in AAD00176 (Ref. 1) Curated2
Sequence conflicti21E → Q in AAD00176 (Ref. 1) Curated1
Sequence conflicti24K → T in AAD00176 (Ref. 1) Curated1
Sequence conflicti32K → T in AAD00176 (Ref. 1) Curated1
Sequence conflicti60I → P in AAD00176 (Ref. 1) Curated1
Sequence conflicti88E → S in AAD00176 (Ref. 1) Curated1
Sequence conflicti222 – 223AS → PA in AAD00176 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70733 mRNA Translation: AAD00176.1
AF108214 mRNA Translation: AAF14221.1
BC008511 mRNA Translation: AAH08511.1
CCDSiCCDS40547.1
RefSeqiNP_058572.2, NM_016876.3
UniGeneiMm.427309

Genome annotation databases

EnsembliENSMUST00000004206; ENSMUSP00000004206; ENSMUSG00000070319
GeneIDi53356
KEGGimmu:53356
UCSCiuc009ojm.1 mouse

Similar proteinsi

Entry informationi

Entry nameiEIF3G_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1D1
Secondary accession number(s): Q9R079
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: September 19, 2002
Last modified: July 18, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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