UniProtKB - Q9Z199 (SPT4B_MOUSE)
Transcription elongation factor SPT4-B
Supt4h1b
Functioni
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites (By similarity).
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 16 – 36 | C4-typeSequence analysisAdd BLAST | 21 |
GO - Molecular functioni
- protein heterodimerization activity Source: MGI
- RNA polymerase II complex binding Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- chromatin organization Source: GO_Central
- mRNA processing Source: GO_Central
- negative regulation of DNA-templated transcription, elongation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of DNA-templated transcription, elongation Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription elongation from RNA polymerase II promoter Source: GO_Central
- transcription elongation from RNA polymerase II promoter Source: MGI
Keywordsi
Molecular function | Activator, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription elongation factor SPT4-BAlternative name(s): DRB sensitivity-inducing factor small subunit 2 Short name: DSIF small subunit 2 Transcription elongation factor SPT4 2 |
Gene namesi | Name:Supt4h1b Synonyms:Gm3258, Supt4b, Supt4h2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1335090, Supt4b |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- DSIF complex Source: UniProtKB
- nucleoplasm Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210328 | 1 – 117 | Transcription elongation factor SPT4-BAdd BLAST | 117 |
Proteomic databases
EPDi | Q9Z199 |
MaxQBi | Q9Z199 |
PRIDEi | Q9Z199 |
ProteomicsDBi | 254548 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Interacts with SUPT5H to form DSIF. DSIF interacts with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of CDK9 and cyclin-T (CCNT1 or CCNT2). DSIF interacts with RNA polymerase II, and this interaction is reduced by phosphorylation of the C-terminal domain (CTD) of POLR2A by P-TEFb. DSIF also interacts with the NELF complex, which is composed of WHSC2/NELFA, COBRA1/NELFB, TH1L/NELFD and RDBP/NELFE, and this interaction occurs following prior binding of DSIF to RNA polymerase II. DSIF also interacts with HRMT1L2/PRMT1, HTATSF1/TATSF1, RNGTT/CAP1A, SKB1/PRMT5, SUPT6H, and can interact with PIN1 (By similarity).
By similarityGO - Molecular functioni
- protein heterodimerization activity Source: MGI
- RNA polymerase II complex binding Source: GO_Central
Protein-protein interaction databases
IntActi | Q9Z199, 1 interactor |
Miscellaneous databases
RNActi | Q9Z199, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 40 | Interaction with SUPT5HBy similarityAdd BLAST | 40 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 16 – 36 | C4-typeSequence analysisAdd BLAST | 21 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
InParanoidi | Q9Z199 |
OrthoDBi | 1617752at2759 |
Family and domain databases
CDDi | cd07973, Spt4, 1 hit |
Gene3Di | 3.30.40.210, 1 hit |
InterProi | View protein in InterPro IPR029040, RPABC4/Spt4 IPR009287, Spt4 IPR022800, Spt4/RpoE2_Znf IPR038510, Spt4_sf |
PANTHERi | PTHR12882, PTHR12882, 1 hit |
Pfami | View protein in Pfam PF06093, Spt4, 1 hit |
PIRSFi | PIRSF025023, Spt4, 1 hit |
SMARTi | View protein in SMART SM01389, Spt4, 1 hit |
SUPFAMi | SSF63393, SSF63393, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MALETVPKDL RHLRACLLCS LVKTIDQFEY DGCDNCDAYL QMKGNREMVY
60 70 80 90 100
DCTSSSFDGI NAMMSPEDSW VSKWQRVSNF KPGVYAVSVT GRLPQGIVRE
110
LKSRGVAYKS RDTAIKT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U96810 Genomic DNA Translation: AAC71660.1 |
RefSeqi | NP_035639.1, NM_011509.2 |
Genome annotation databases
GeneIDi | 100041294 |
KEGGi | mmu:100041294 |
UCSCi | uc011xck.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U96810 Genomic DNA Translation: AAC71660.1 |
RefSeqi | NP_035639.1, NM_011509.2 |
3D structure databases
SMRi | Q9Z199 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q9Z199, 1 interactor |
Proteomic databases
EPDi | Q9Z199 |
MaxQBi | Q9Z199 |
PRIDEi | Q9Z199 |
ProteomicsDBi | 254548 |
Protocols and materials databases
DNASUi | 100041294 |
Genome annotation databases
GeneIDi | 100041294 |
KEGGi | mmu:100041294 |
UCSCi | uc011xck.1, mouse |
Organism-specific databases
CTDi | 100041294 |
MGIi | MGI:1335090, Supt4b |
Phylogenomic databases
InParanoidi | Q9Z199 |
OrthoDBi | 1617752at2759 |
Miscellaneous databases
BioGRID-ORCSi | 100041294, 4 hits in 10 CRISPR screens |
PROi | PR:Q9Z199 |
RNActi | Q9Z199, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd07973, Spt4, 1 hit |
Gene3Di | 3.30.40.210, 1 hit |
InterProi | View protein in InterPro IPR029040, RPABC4/Spt4 IPR009287, Spt4 IPR022800, Spt4/RpoE2_Znf IPR038510, Spt4_sf |
PANTHERi | PTHR12882, PTHR12882, 1 hit |
Pfami | View protein in Pfam PF06093, Spt4, 1 hit |
PIRSFi | PIRSF025023, Spt4, 1 hit |
SMARTi | View protein in SMART SM01389, Spt4, 1 hit |
SUPFAMi | SSF63393, SSF63393, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SPT4B_MOUSE | |
Accessioni | Q9Z199Primary (citable) accession number: Q9Z199 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 2002 |
Last sequence update: | May 1, 1999 | |
Last modified: | February 23, 2022 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families