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Protein

Eyes absent homolog 4

Gene

Eya4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei352NucleophileBy similarity1
Metal bindingi352MagnesiumBy similarity1
Active sitei354Proton donorBy similarity1
Metal bindingi354Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi580MagnesiumBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein tyrosine phosphatase activity Source: GO_Central
  • transcription factor binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionActivator, Chromatin regulator, Developmental protein, Hydrolase, Protein phosphatase
Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks

Names & Taxonomyi

Protein namesi
Recommended name:
Eyes absent homolog 4 (EC:3.1.3.48By similarity)
Gene namesi
Name:Eya4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1337104 Eya4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002186521 – 616Eyes absent homolog 4Add BLAST616

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Cross-linki14Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki52Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei338PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9Z191
PaxDbiQ9Z191
PeptideAtlasiQ9Z191
PRIDEiQ9Z191

PTM databases

iPTMnetiQ9Z191
PhosphoSitePlusiQ9Z191

Expressioni

Tissue specificityi

In the embryo, expressed mainly in the craniofacial mesenchyme, dermamyotome and limb.1 Publication

Gene expression databases

BgeeiENSMUSG00000010461 Expressed in 208 organ(s), highest expression level in epithelium of cochlear duct
CleanExiMM_EYA4
ExpressionAtlasiQ9Z191 baseline and differential
GenevisibleiQ9Z191 MM

Interactioni

Subunit structurei

Interacts with SIX3; translocates EYA4 from the cytoplasm to the nucleus and promotes activation of their target genes.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199562, 1 interactor
MINTiQ9Z191
STRINGi10090.ENSMUSP00000090335

Structurei

3D structure databases

ProteinModelPortaliQ9Z191
SMRiQ9Z191
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3107 Eukaryota
ENOG410XT12 LUCA
GeneTreeiENSGT00390000008860
HOGENOMiHOG000293149
HOVERGENiHBG002447
InParanoidiQ9Z191
KOiK17622

Family and domain databases

CDDicd02601 HAD_Eya, 1 hit
Gene3Di3.40.50.12350, 1 hit
InterProiView protein in InterPro
IPR028478 EYA4
IPR006545 EYA_dom
IPR038102 EYA_dom_sf
IPR028472 EYA_fam
PANTHERiPTHR10190 PTHR10190, 1 hit
PTHR10190:SF17 PTHR10190:SF17, 1 hit
TIGRFAMsiTIGR01658 EYA-cons_domain, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9Z191-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEDTQDLNEQ SVKKTCPEAD VSEPQNSRSM EMQDLASPHA LVGGSDTPGS
60 70 80 90 100
SKLDKSGLSS TSVTTNGTGV SLLAVKTEPL HSSESTTTTG DGALDTFTGS
110 120 130 140 150
VITSSGYSPR SAQQYSPQLY PSKPYPHILS TPAAQTMSAY AGQTQYSGMQ
160 170 180 190 200
QPAVYTAYSQ TGQPYSLPAY DLGVMLPAIK TESGLSQTQS PLQSGCLSYS
210 220 230 240 250
PGFSTPQPGQ TPYSYQMPGS SFAPSSTIYA NNSVSNSTNF SSSQQDYPSY
260 270 280 290 300
TAFGQNQYAQ YYSASTYGAY MTSNNTADGT SSSTSTYQLQ ESLQGLTSQP
310 320 330 340 350
GEFDTVQSPS TPIKDLDDRT CRSSGSKSRG RGRKNNPSPP PDSDLERVFV
360 370 380 390 400
WDLDETIIVF HSLLTGSYAQ KYGKDPPMAV TLGLRMEEMI FNLADTHLFF
410 420 430 440 450
NDLEECDQVH IDDVSSDDNG QDLSTYSFAT DGFHAAASSA NLCLPTGVRG
460 470 480 490 500
GVDWMRKLAF RYRRVKELYN TYKNNVGGLL GPAKRDAWLQ LRAEIEGLTD
510 520 530 540 550
SWLTNALKSL SIISTRSNCV NVLVTTTQLI PALAKVLLYS LGGAFPIENI
560 570 580 590 600
YSATKIGKES CFERIVSRFG TNITYVVIGD GRDEEHAANQ HNMPFWRISS
610
HSDLLALHQA LELEYL
Length:616
Mass (Da):66,875
Last modified:July 27, 2011 - v2
Checksum:iAC4B7E891AB7A6F3
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8BY78Q8BY78_MOUSE
Eyes absent homolog
Eya4
616Annotation score:
A0A1W2P721A0A1W2P721_MOUSE
Eyes absent homolog
Eya4
639Annotation score:
A0A1W2P897A0A1W2P897_MOUSE
Eyes absent homolog
Eya4
74Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti337P → L in CAA76637 (PubMed:9887327).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17115 mRNA Translation: CAA76637.1
AJ007997 mRNA Translation: CAA07820.1
CH466540 Genomic DNA Translation: EDL04766.1
BC120899 mRNA Translation: AAI20900.1
CCDSiCCDS83687.1
RefSeqiNP_001334301.1, NM_001347372.1
XP_006512586.1, XM_006512523.3
XP_006512587.1, XM_006512524.3
UniGeneiMm.332128
Mm.446298

Genome annotation databases

EnsembliENSMUST00000074366; ENSMUSP00000073970; ENSMUSG00000010461
ENSMUST00000220299; ENSMUSP00000151287; ENSMUSG00000010461
GeneIDi14051
KEGGimmu:14051
UCSCiuc007epu.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17115 mRNA Translation: CAA76637.1
AJ007997 mRNA Translation: CAA07820.1
CH466540 Genomic DNA Translation: EDL04766.1
BC120899 mRNA Translation: AAI20900.1
CCDSiCCDS83687.1
RefSeqiNP_001334301.1, NM_001347372.1
XP_006512586.1, XM_006512523.3
XP_006512587.1, XM_006512524.3
UniGeneiMm.332128
Mm.446298

3D structure databases

ProteinModelPortaliQ9Z191
SMRiQ9Z191
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199562, 1 interactor
MINTiQ9Z191
STRINGi10090.ENSMUSP00000090335

PTM databases

iPTMnetiQ9Z191
PhosphoSitePlusiQ9Z191

Proteomic databases

MaxQBiQ9Z191
PaxDbiQ9Z191
PeptideAtlasiQ9Z191
PRIDEiQ9Z191

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074366; ENSMUSP00000073970; ENSMUSG00000010461
ENSMUST00000220299; ENSMUSP00000151287; ENSMUSG00000010461
GeneIDi14051
KEGGimmu:14051
UCSCiuc007epu.1 mouse

Organism-specific databases

CTDi2070
MGIiMGI:1337104 Eya4

Phylogenomic databases

eggNOGiKOG3107 Eukaryota
ENOG410XT12 LUCA
GeneTreeiENSGT00390000008860
HOGENOMiHOG000293149
HOVERGENiHBG002447
InParanoidiQ9Z191
KOiK17622

Enzyme and pathway databases

ReactomeiR-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks

Miscellaneous databases

ChiTaRSiEya4 mouse
PROiPR:Q9Z191
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000010461 Expressed in 208 organ(s), highest expression level in epithelium of cochlear duct
CleanExiMM_EYA4
ExpressionAtlasiQ9Z191 baseline and differential
GenevisibleiQ9Z191 MM

Family and domain databases

CDDicd02601 HAD_Eya, 1 hit
Gene3Di3.40.50.12350, 1 hit
InterProiView protein in InterPro
IPR028478 EYA4
IPR006545 EYA_dom
IPR038102 EYA_dom_sf
IPR028472 EYA_fam
PANTHERiPTHR10190 PTHR10190, 1 hit
PTHR10190:SF17 PTHR10190:SF17, 1 hit
TIGRFAMsiTIGR01658 EYA-cons_domain, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEYA4_MOUSE
AccessioniPrimary (citable) accession number: Q9Z191
Secondary accession number(s): Q0VAV8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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