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Entry version 191 (07 Oct 2020)
Sequence version 2 (15 Nov 2002)
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Protein

Histone-lysine N-methyltransferase EHMT2

Gene

Ehmt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.4 Publications

Caution

NG36 and G9a were originally thought to derive from two separate genes.Curated

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi1027Zinc 1By similarity1
Metal bindingi1027Zinc 2By similarity1
Metal bindingi1029Zinc 1By similarity1
Metal bindingi1033Zinc 1By similarity1
Metal bindingi1033Zinc 3By similarity1
Metal bindingi1038Zinc 1By similarity1
Metal bindingi1040Zinc 2By similarity1
Metal bindingi1070Zinc 2By similarity1
Metal bindingi1070Zinc 3By similarity1
Metal bindingi1074Zinc 2By similarity1
Metal bindingi1076Zinc 3By similarity1
Metal bindingi1080Zinc 3By similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei1120Histone H3K9meBy similarity1
Binding sitei1138S-adenosyl-L-methioninePROSITE-ProRule annotation1
Metal bindingi1168Zinc 4By similarity1
Metal bindingi1221Zinc 4By similarity1
Metal bindingi1223Zinc 4By similarity1
Metal bindingi1228Zinc 4By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionChromatin regulator, Methyltransferase, Transferase
LigandMetal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
1.97.1.10, 3474

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-MMU-2559582, Senescence-Associated Secretory Phenotype (SASP)
R-MMU-3214841, PKMTs methylate histone lysines
R-MMU-6804760, Regulation of TP53 Activity through Methylation
R-MMU-73762, RNA Polymerase I Transcription Initiation
R-MMU-8953750, Transcriptional Regulation by E2F6

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Histone-lysine N-methyltransferase EHMT2 (EC:2.1.1.-2 Publications)
Alternative name(s):
Euchromatic histone-lysine N-methyltransferase 2
HLA-B-associated transcript 8
Histone H3-K9 methyltransferase 3
Short name:
H3-K9-HMTase 3
Protein G9a
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Ehmt2
Synonyms:Bat8, G9a, Ng36
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 17

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:2148922, Ehmt2

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Mice show a higher level of histone H3 with acetylated 'Lys-9' (H3K9ac) and/or methylated 'Lys-4' (H3K4me), display severe developmental defects and die within E9.5-E12.5 stages.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi1120Y → V in GM7; defective in methyltransferase activity without affecting DNA methylation; when associated with F-1207. 1 Publication1
Mutagenesisi1162R → H: Strongly reduces histone methyltransferase activity. 1 Publication1
Mutagenesisi1165 – 1168Missing : Abolishes histone methyltransferase activity and subsequent repression. 2 Publications4
Mutagenesisi1165 – 1168Missing in GM3; does not form heterodimer with EHMT1 and is defective in mediating both H3K9me and DNA methylation. 2 Publications4
Mutagenesisi1165 – 1166NH → LE in GM6; does not form heterodimer with EHMT1 and is defective in mediating H3K9me. 1 Publication2
Mutagenesisi1168C → A in GM4; defective in methyltransferase activity without affecting DNA methylation. 1 Publication1
Mutagenesisi1207Y → F in GM7; defective in methyltransferase activity without affecting DNA methylation; when associated with V-1120. 1 Publication1

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...
ChEMBLi
CHEMBL2169718

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001860691 – 1263Histone-lysine N-methyltransferase EHMT2Add BLAST1263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei40Asymmetric dimethylarginineCombined sources1
Modified residuei97PhosphoserineBy similarity1
Modified residuei101PhosphothreonineBy similarity1
Modified residuei104PhosphoserineBy similarity1
Modified residuei193PhosphoserineCombined sources1
Modified residuei239N6,N6,N6-trimethyllysine; by EHMT2; alternateBy similarity1
Modified residuei239N6,N6-dimethyllysine; by EHMT2; alternateBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki272Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki282Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei285PhosphoserineBy similarity1
Modified residuei294PhosphoserineBy similarity1
Modified residuei298PhosphoserineBy similarity1
Modified residuei403PhosphoserineBy similarity1
Modified residuei465PhosphoserineCombined sources1
Modified residuei466PhosphoserineCombined sources1
Modified residuei608PhosphothreonineBy similarity1
Cross-linki687Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1257PhosphoserineBy similarity1
Modified residuei1263PhosphothreonineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Methylated at Lys-239; automethylated.By similarity

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
Q9Z148

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q9Z148

PeptideAtlas

More...
PeptideAtlasi
Q9Z148

PRoteomics IDEntifications database

More...
PRIDEi
Q9Z148

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q9Z148

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q9Z148

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000013787, Expressed in neocortex and 330 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q9Z148, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q9Z148, MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EHMT1, RING1, RNF2, MBLR, GFI1B, L3MBTL2 and YAF2 (By similarity). Heterodimer; heterodimerizes with EHMT1.

Interacts with WIZ.

Interacts with PRDM9 and CDYL; interaction only takes place when PRDM9 is bound to hotspot DNA (PubMed:27932493).

By similarity4 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
225335, 60 interactors

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
Q9Z148

Database of interacting proteins

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DIPi
DIP-31916N

Protein interaction database and analysis system

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IntActi
Q9Z148, 18 interactors

Molecular INTeraction database

More...
MINTi
Q9Z148

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000013931

Chemistry databases

BindingDB database of measured binding affinities

More...
BindingDBi
Q9Z148

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

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RNActi
Q9Z148, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q9Z148

Database of comparative protein structure models

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ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati702 – 731ANK 1Add BLAST30
Repeati737 – 766ANK 2Add BLAST30
Repeati770 – 799ANK 3Add BLAST30
Repeati803 – 833ANK 4Add BLAST31
Repeati837 – 866ANK 5Add BLAST30
Repeati870 – 899ANK 6Add BLAST30
Repeati903 – 932ANK 7Add BLAST30
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini1025 – 1088Pre-SETPROSITE-ProRule annotationAdd BLAST64
Domaini1091 – 1208SETPROSITE-ProRule annotationAdd BLAST118
Domaini1217 – 1233Post-SETAdd BLAST17

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni870 – 872Histone H3K9me bindingBy similarity3
Regioni1101 – 1103S-adenosyl-L-methionine bindingBy similarity3
Regioni1127 – 1146Interaction with histone H3By similarityAdd BLAST20
Regioni1165 – 1166S-adenosyl-L-methionine bindingBy similarity2
Regioni1207 – 1210Interaction with histone H3By similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi352 – 379Poly-GluAdd BLAST28

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The ANK repeats bind H3K9me1 and H3K9me2.By similarity
The SET domain mediates interaction with WIZ.
In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.By similarity

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG1082, Eukaryota

Ensembl GeneTree

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GeneTreei
ENSGT00940000159459

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
CLU_005790_0_0_1

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q9Z148

KEGG Orthology (KO)

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KOi
K11420

Database of Orthologous Groups

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OrthoDBi
753093at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q9Z148

TreeFam database of animal gene trees

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TreeFami
TF106443

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
1.25.40.20, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002110, Ankyrin_rpt
IPR020683, Ankyrin_rpt-contain_dom
IPR036770, Ankyrin_rpt-contain_sf
IPR043550, EHMT1/EHMT2
IPR038034, EHMT2
IPR007728, Pre-SET_dom
IPR001214, SET_dom

The PANTHER Classification System

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PANTHERi
PTHR46307, PTHR46307, 1 hit
PTHR46307:SF1, PTHR46307:SF1, 1 hit

Pfam protein domain database

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Pfami
View protein in Pfam
PF12796, Ank_2, 3 hits
PF05033, Pre-SET, 1 hit
PF00856, SET, 1 hit

Protein Motif fingerprint database; a protein domain database

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PRINTSi
PR01415, ANKYRIN

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00248, ANK, 6 hits
SM00468, PreSET, 1 hit
SM00317, SET, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48403, SSF48403, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50297, ANK_REP_REGION, 1 hit
PS50088, ANK_REPEAT, 5 hits
PS50867, PRE_SET, 1 hit
PS50280, SET, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Z148-1) [UniParc]FASTAAdd to basket
Also known as: G9a-L

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRGLPRGRGL MRARGRGRAA PTGGRGRGRG GAHRGRGRPR SLLSLPRAQA
60 70 80 90 100
SWAPQLPAGL TGPPVPCLPS QGEAPAEMGA LLLEKEPRGA AERVHSSLGD
110 120 130 140 150
TPQSEETLPK ANPDSLEPAG PSSPASVTVT VGDEGADTPV GAASLIGDEP
160 170 180 190 200
ESLEGDGGRI VLGHATKSFP SSPSKGGACP SRAKMSMTGA GKSPPSVQSL
210 220 230 240 250
AMRLLSMPGA QGAATAGPEP SPATTAAQEG QPKVHRARKT MSKPSNGQPP
260 270 280 290 300
IPEKRPPEVQ HFRMSDDMHL GKVTSDVAKR RKLNSGSLSE DLGSAGGSGD
310 320 330 340 350
IILEKGEPRP LEEWETVVGD DFSLYYDAYS VDERVDSDSK SEVEALAEQL
360 370 380 390 400
SEEEEEEEEE EEEEEEEEEE EEEEEEDEES GNQSDRSGSS GRRKAKKKWR
410 420 430 440 450
KDSPWVKPSR KRRKREPPRA KEPRGVNGVG SSGPSEYMEV PLGSLELPSE
460 470 480 490 500
GTLSPNHAGV SNDTSSLETE RGFEELPLCS CRMEAPKIDR ISERAGHKCM
510 520 530 540 550
ATESVDGELL GCNAAILKRE TMRPSSRVAL MVLCEAHRAR MVKHHCCPGC
560 570 580 590 600
GYFCTAGTFL ECHPDFRVAH RFHKACVSQL NGMVFCPHCG EDASEAQEVT
610 620 630 640 650
IPRGDGGTPP IGTAAPALPP LAHDAPGRAD TSQPSARMRG HGEPRRPPCD
660 670 680 690 700
PLADTIDSSG PSLTLPNGGC LSAVGLPPGP GREALEKALV IQESERRKKL
710 720 730 740 750
RFHPRQLYLS VKQGELQKVI LMLLDNLDPN FQSDQQSKRT PLHAAAQKGS
760 770 780 790 800
VEICHVLLQA GANINAVDKQ QRTPLMEAVV NNHLEVARYM VQLGGCVYSK
810 820 830 840 850
EEDGSTCLHH AAKIGNLEMV SLLLSTGQVD VNAQDSGGWT PIIWAAEHKH
860 870 880 890 900
IDVIRMLLTR GADVTLTDNE ENICLHWASF TGSAAIAEVL LNAQCDLHAV
910 920 930 940 950
NYHGDTPLHI AARESYHDCV LLFLSRGANP ELRNKEGDTA WDLTPERSDV
960 970 980 990 1000
WFALQLNRKL RLGVGNRAVR TEKIICRDVA RGYENVPIPC VNGVDGEPCP
1010 1020 1030 1040 1050
EDYKYISENC ETSTMNIDRN ITHLQHCTCV DDCSSSNCLC GQLSIRCWYD
1060 1070 1080 1090 1100
KDGRLLQEFN KIEPPLIFEC NQACSCWRSC KNRVVQSGIK VRLQLYRTAK
1110 1120 1130 1140 1150
MGWGVRALQT IPQGTFICEY VGELISDAEA DVREDDSYLF DLDNKDGEVY
1160 1170 1180 1190 1200
CIDARYYGNI SRFINHLCDP NIIPVRVFML HQDLRFPRIA FFSSRDIRTG
1210 1220 1230 1240 1250
EELGFDYGDR FWDIKSKYFT CQCGSEKCKH SAEAIALEQS RLARLDPHPE
1260
LLPDLSSLPP INT
Length:1,263
Mass (Da):138,039
Last modified:November 15, 2002 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i74DBFF9A36769589
GO
Isoform 2 (identifier: Q9Z148-2) [UniParc]FASTAAdd to basket
Also known as: G9a-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: Missing.
     58-71: AGLTGPPVPCLPSQ → MAAAAGAAAAAAAE
     426-459: Missing.

Show »
Length:1,172
Mass (Da):128,420
Checksum:i52D345057AFCF200
GO
Isoform 3 (identifier: Q9Z148-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: Missing.
     58-71: AGLTGPPVPCLPSQ → MAAAAGAAAAAAAE

Show »
Length:1,206
Mass (Da):131,772
Checksum:i5DFF4CBADB784977
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2CG76A2CG76_MOUSE
Histone-lysine N-methyltransferase ...
Ehmt2
1,229Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H3BIW5H3BIW5_MOUSE
Histone-lysine N-methyltransferase ...
Ehmt2
155Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A2CG77A2CG77_MOUSE
Histone-lysine N-methyltransferase ...
Ehmt2
441Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
G3UYH7G3UYH7_MOUSE
Histone-lysine N-methyltransferase ...
Ehmt2
194Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAC84164 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAC84165 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAH25539 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAH58357 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0022141 – 57Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST57
Alternative sequenceiVSP_00221558 – 71AGLTG…CLPSQ → MAAAAGAAAAAAAE in isoform 2 and isoform 3. 2 PublicationsAdd BLAST14
Alternative sequenceiVSP_002216426 – 459Missing in isoform 2. 1 PublicationAdd BLAST34

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF109906 Genomic DNA Translation: AAC84164.1 Sequence problems.
AF109906 Genomic DNA Translation: AAC84165.1 Sequence problems.
AB077209 mRNA Translation: BAC05482.1
AB077210 mRNA Translation: BAC05483.1
CT025759 Genomic DNA No translation available.
BC025539 mRNA Translation: AAH25539.1 Different initiation.
BC058357 mRNA Translation: AAH58357.1 Different initiation.

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS28666.1 [Q9Z148-2]
CCDS28667.1 [Q9Z148-1]
CCDS70797.1 [Q9Z148-3]

NCBI Reference Sequences

More...
RefSeqi
NP_001273502.1, NM_001286573.1
NP_001273504.1, NM_001286575.1 [Q9Z148-3]
NP_665829.1, NM_145830.2 [Q9Z148-1]
NP_671493.1, NM_147151.2 [Q9Z148-2]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSMUST00000013931; ENSMUSP00000013931; ENSMUSG00000013787 [Q9Z148-1]
ENSMUST00000078061; ENSMUSP00000077208; ENSMUSG00000013787 [Q9Z148-2]
ENSMUST00000114033; ENSMUSP00000109667; ENSMUSG00000013787 [Q9Z148-3]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
110147

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mmu:110147

UCSC genome browser

More...
UCSCi
uc008ceb.3, mouse [Q9Z148-2]
uc008cec.3, mouse [Q9Z148-3]
uc008ced.3, mouse [Q9Z148-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109906 Genomic DNA Translation: AAC84164.1 Sequence problems.
AF109906 Genomic DNA Translation: AAC84165.1 Sequence problems.
AB077209 mRNA Translation: BAC05482.1
AB077210 mRNA Translation: BAC05483.1
CT025759 Genomic DNA No translation available.
BC025539 mRNA Translation: AAH25539.1 Different initiation.
BC058357 mRNA Translation: AAH58357.1 Different initiation.
CCDSiCCDS28666.1 [Q9Z148-2]
CCDS28667.1 [Q9Z148-1]
CCDS70797.1 [Q9Z148-3]
RefSeqiNP_001273502.1, NM_001286573.1
NP_001273504.1, NM_001286575.1 [Q9Z148-3]
NP_665829.1, NM_145830.2 [Q9Z148-1]
NP_671493.1, NM_147151.2 [Q9Z148-2]

3D structure databases

SMRiQ9Z148
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi225335, 60 interactors
CORUMiQ9Z148
DIPiDIP-31916N
IntActiQ9Z148, 18 interactors
MINTiQ9Z148
STRINGi10090.ENSMUSP00000013931

Chemistry databases

BindingDBiQ9Z148
ChEMBLiCHEMBL2169718

PTM databases

iPTMnetiQ9Z148
PhosphoSitePlusiQ9Z148

Proteomic databases

jPOSTiQ9Z148
PaxDbiQ9Z148
PeptideAtlasiQ9Z148
PRIDEiQ9Z148

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...
Antibodypediai
27888, 350 antibodies

Genome annotation databases

EnsembliENSMUST00000013931; ENSMUSP00000013931; ENSMUSG00000013787 [Q9Z148-1]
ENSMUST00000078061; ENSMUSP00000077208; ENSMUSG00000013787 [Q9Z148-2]
ENSMUST00000114033; ENSMUSP00000109667; ENSMUSG00000013787 [Q9Z148-3]
GeneIDi110147
KEGGimmu:110147
UCSCiuc008ceb.3, mouse [Q9Z148-2]
uc008cec.3, mouse [Q9Z148-3]
uc008ced.3, mouse [Q9Z148-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
10919
MGIiMGI:2148922, Ehmt2

Phylogenomic databases

eggNOGiKOG1082, Eukaryota
GeneTreeiENSGT00940000159459
HOGENOMiCLU_005790_0_0_1
InParanoidiQ9Z148
KOiK11420
OrthoDBi753093at2759
PhylomeDBiQ9Z148
TreeFamiTF106443

Enzyme and pathway databases

BRENDAi1.97.1.10, 3474
ReactomeiR-MMU-2559582, Senescence-Associated Secretory Phenotype (SASP)
R-MMU-3214841, PKMTs methylate histone lysines
R-MMU-6804760, Regulation of TP53 Activity through Methylation
R-MMU-73762, RNA Polymerase I Transcription Initiation
R-MMU-8953750, Transcriptional Regulation by E2F6

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
110147, 6 hits in 23 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
Ehmt2, mouse

Protein Ontology

More...
PROi
PR:Q9Z148
RNActiQ9Z148, protein

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000013787, Expressed in neocortex and 330 other tissues
ExpressionAtlasiQ9Z148, baseline and differential
GenevisibleiQ9Z148, MM

Family and domain databases

Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110, Ankyrin_rpt
IPR020683, Ankyrin_rpt-contain_dom
IPR036770, Ankyrin_rpt-contain_sf
IPR043550, EHMT1/EHMT2
IPR038034, EHMT2
IPR007728, Pre-SET_dom
IPR001214, SET_dom
PANTHERiPTHR46307, PTHR46307, 1 hit
PTHR46307:SF1, PTHR46307:SF1, 1 hit
PfamiView protein in Pfam
PF12796, Ank_2, 3 hits
PF05033, Pre-SET, 1 hit
PF00856, SET, 1 hit
PRINTSiPR01415, ANKYRIN
SMARTiView protein in SMART
SM00248, ANK, 6 hits
SM00468, PreSET, 1 hit
SM00317, SET, 1 hit
SUPFAMiSSF48403, SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297, ANK_REP_REGION, 1 hit
PS50088, ANK_REPEAT, 5 hits
PS50867, PRE_SET, 1 hit
PS50280, SET, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiEHMT2_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9Z148
Secondary accession number(s): A2CG75
, Q6PE08, Q8K4R6, Q8K4R7, Q9Z149
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 15, 2002
Last modified: October 7, 2020
This is version 191 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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