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Protein

ATP-dependent DNA helicase Q1

Gene

Recql

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Isoform alpha is a DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Isoform beta may have important roles in the meiotic process. Both isoforms exhibit a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi113 – 120ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase Q1 (EC:3.6.4.12)
Alternative name(s):
DNA-dependent ATPase Q1
RecQ protein-like 1
Gene namesi
Name:Recql
Synonyms:Recql1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:103021 Recql

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002050501 – 648ATP-dependent DNA helicase Q1Add BLAST648

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei514N6-acetyllysineBy similarity1
Modified residuei522N6-acetyllysineBy similarity1
Modified residuei597PhosphoserineBy similarity1
Modified residuei633PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Z129
PaxDbiQ9Z129
PRIDEiQ9Z129

PTM databases

iPTMnetiQ9Z129
PhosphoSitePlusiQ9Z129

Expressioni

Tissue specificityi

Isoform alpha is expressed in all tissues examined. Isoform beta is only expressed in spermatocytes, expression increases at pachytene (17 days old) and decreases after completion of meiosis II (7 weeks old).1 Publication

Gene expression databases

BgeeiENSMUSG00000030243 Expressed in 270 organ(s), highest expression level in testis
CleanExiMM_RECQL
ExpressionAtlasiQ9Z129 baseline and differential
GenevisibleiQ9Z129 MM

Interactioni

Subunit structurei

Interacts with EXO1 and MLH1.By similarity

Protein-protein interaction databases

BioGridi202847, 2 interactors
STRINGi10090.ENSMUSP00000107434

Structurei

3D structure databases

ProteinModelPortaliQ9Z129
SMRiQ9Z129
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini100 – 275Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST176
Domaini299 – 451Helicase C-terminalPROSITE-ProRule annotationAdd BLAST153

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi219 – 222DEVH box4

Sequence similaritiesi

Belongs to the helicase family. RecQ subfamily.Curated

Phylogenomic databases

eggNOGiKOG0351 Eukaryota
COG0514 LUCA
GeneTreeiENSGT00550000074520
HOGENOMiHOG000044388
HOVERGENiHBG057654
InParanoidiQ9Z129
KOiK10899
OMAiESSQTCH
OrthoDBiEOG091G05Q8
TreeFamiTF323555

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR004589 DNA_helicase_ATP-dep_RecQ
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR032284 RecQ_Zn-bd
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF16124 RecQ_Zn_bind, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
TIGRFAMsiTIGR00614 recQ_fam, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.iShow all

Isoform Alpha (identifier: Q9Z129-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASVSALTEE LESVASELHA IDIQIQELTE RRQELLQRKS VLTGKIKQYL
60 70 80 90 100
EDSSAEASSD LDTSPAAWNK EDFPWFGKVK DVLQNVFKLQ KFRPLQLETI
110 120 130 140 150
NVTMARKDIF LVMPTGGGKS LCYQLPALCS DGFTLVICPL ISLMEDQLMV
160 170 180 190 200
LKQLGISATM LNASSSKEHV KWVHAEMVNK NSQLKLIYVT PEKIAKSKMF
210 220 230 240 250
MSRLEKAYEA GRLTGAAVDE VHCCSQWGHD FRPDYKALGI LKRQFPNASL
260 270 280 290 300
MGLTATATNH VLKDVQKILC VGKCLTFTAS FNRPNLFYEV RQKPSSAEDF
310 320 330 340 350
TEDIVKLING RYKGQSGIIY CFSQKDSEQI TISLQKLGIH AGTYHANMEP
360 370 380 390 400
EDKTKVHTQW SANELQVVVA TVAFGMGIDK PDVRFVIHHS MSKSMENYYQ
410 420 430 440 450
ESGRAGRDDS RADCILYYGF GDIFRISSMV VMENVGQQKL YEMVSYCQNV
460 470 480 490 500
SKCRRVLIAQ HFDEVWNADA CNKMCDNCCK DVSFEKKNVT QHCRDLIKIL
510 520 530 540 550
KQAEGLNEKL TPLKLIDAWM GKGAAKLRVA GVVAPALPRE DLERIVAHAL
560 570 580 590 600
LQQYLKEDYS FTAYATISYL KVGPRACLLS NEAHAVTMQV KKSAQSSVRG
610 620 630 640
ALSEARQVEQ VDSKGEEQSS GNSQKSKSRL QPSGSKNAGA KKRKLDDA
Length:648
Mass (Da):72,484
Last modified:July 27, 2011 - v2
Checksum:iDF84B5E9097BF3F3
GO
Isoform Beta (identifier: Q9Z129-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     622-648: NSQKSKSRLQPSGSKNAGAKKRKLDDA → SHAADTAANV

Show »
Length:631
Mass (Da):70,540
Checksum:i633BAB8A1CA86761
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q3N0E9Q3N0_MOUSE
ATP-dependent DNA helicase
Recql
634Annotation score:
D3Z6T0D3Z6T0_MOUSE
ATP-dependent DNA helicase Q1
Recql
167Annotation score:
F6S4D9F6S4D9_MOUSE
ATP-dependent DNA helicase Q1
Recql
235Annotation score:
D3YZW9D3YZW9_MOUSE
ATP-dependent DNA helicase Q1
Recql
85Annotation score:
F7DB73F7DB73_MOUSE
ATP-dependent DNA helicase Q1
Recql
61Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti216A → V in BAA75085 (PubMed:9838113).Curated1
Sequence conflicti216A → V in BAA75086 (PubMed:9838113).Curated1
Sequence conflicti518A → T in BAA75085 (PubMed:9838113).Curated1
Sequence conflicti518A → T in BAA75086 (PubMed:9838113).Curated1
Sequence conflicti525A → P in BAA75085 (PubMed:9838113).Curated1
Sequence conflicti525A → P in BAA75086 (PubMed:9838113).Curated1
Sequence conflicti602L → P in BAA75085 (PubMed:9838113).Curated1
Sequence conflicti602L → P in BAA75086 (PubMed:9838113).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005567622 – 648NSQKS…KLDDA → SHAADTAANV in isoform Beta. CuratedAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017104 mRNA Translation: BAA75085.1
AB017105 mRNA Translation: BAA75086.1
AK137589 mRNA Translation: BAE23422.1
AK164344 mRNA Translation: BAE37751.1
CH466572 Genomic DNA Translation: EDL10638.1
CCDSiCCDS39695.1 [Q9Z129-1]
CCDS57465.1 [Q9Z129-2]
RefSeqiNP_001191836.1, NM_001204907.1 [Q9Z129-2]
NP_075529.2, NM_023042.3 [Q9Z129-1]
XP_017176957.1, XM_017321468.1 [Q9Z129-1]
XP_017176958.1, XM_017321469.1
UniGeneiMm.27407

Genome annotation databases

EnsembliENSMUST00000032370; ENSMUSP00000032370; ENSMUSG00000030243 [Q9Z129-2]
ENSMUST00000111803; ENSMUSP00000107434; ENSMUSG00000030243 [Q9Z129-1]
GeneIDi19691
KEGGimmu:19691
UCSCiuc009epf.2 mouse [Q9Z129-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRECQ1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z129
Secondary accession number(s): Q3TPI5, Q9Z128
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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