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Protein

Large neutral amino acids transporter small subunit 1

Gene

Slc7a5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. When associated with LAPTM4B, recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (By similarity).By similarity3 Publications

Miscellaneous

Leucine uptake was inhibited by ileum, valine histidine and phenylalanine as well as by 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH) (a specific inhibitor of system L transport).

Kineticsi

  1. KM=25 µM for leucine1 Publication

    GO - Molecular functioni

    • L-amino acid transmembrane transporter activity Source: MGI

    GO - Biological processi

    Keywordsi

    Molecular functionDevelopmental protein
    Biological processAmino-acid transport, Differentiation, Neurogenesis, Transport

    Enzyme and pathway databases

    ReactomeiR-MMU-210991 Basigin interactions
    R-MMU-352230 Amino acid transport across the plasma membrane
    R-MMU-71240 Tryptophan catabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large neutral amino acids transporter small subunit 1
    Alternative name(s):
    4F2 light chain
    Short name:
    4F2 LC
    Short name:
    4F2LC
    L-type amino acid transporter 1
    Solute carrier family 7 member 5
    Gene namesi
    Name:Slc7a5
    Synonyms:Lat1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 8

    Organism-specific databases

    MGIiMGI:1298205 Slc7a5

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei51 – 71HelicalSequence analysisAdd BLAST21
    Transmembranei85 – 105HelicalSequence analysisAdd BLAST21
    Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
    Transmembranei147 – 167HelicalSequence analysisAdd BLAST21
    Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
    Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
    Transmembranei248 – 268HelicalSequence analysisAdd BLAST21
    Transmembranei279 – 299HelicalSequence analysisAdd BLAST21
    Transmembranei324 – 344HelicalSequence analysisAdd BLAST21
    Transmembranei398 – 418HelicalSequence analysisAdd BLAST21
    Transmembranei436 – 456HelicalSequence analysisAdd BLAST21
    Transmembranei463 – 483HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000542711 – 512Large neutral amino acids transporter small subunit 1Add BLAST512

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei46PhosphothreonineBy similarity1
    Glycosylationi50N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi345N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    EPDiQ9Z127
    MaxQBiQ9Z127
    PaxDbiQ9Z127
    PRIDEiQ9Z127

    PTM databases

    iPTMnetiQ9Z127
    PhosphoSitePlusiQ9Z127
    SwissPalmiQ9Z127

    Expressioni

    Tissue specificityi

    Predominantly expressed in the microvessels in the brain parenchyma of the central nervous system. Also detected in the subfornical organ, the subcommissural organ, ventromedial nucleus of the hypothalamus, subgranular zone of the dentate gyrus in hippocampus, ependymal layer of the lateral ventricles, and the olfactory bulb. Very strong expression also seen in testis, ovary, and placenta with weaker expression in spleen, skin, brain, thymus, stomach, lung, heart, kidney, small intestine, uterus and skeletal muscle.4 Publications

    Developmental stagei

    Strong expression in the liver of 14 dpc embryo. In embryo of 18 dpc expressed strongly in brain, moderate expression in spleen and brain and weak expression in liver.1 Publication

    Inductioni

    Expression induced by concanavilin-A stimulation.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000040010 Expressed in 286 organ(s), highest expression level in brain blood vessel
    GenevisibleiQ9Z127 MM

    Interactioni

    Subunit structurei

    Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc (PubMed:9915839). Interacts with LAPTM4B; recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (By similarity).By similarity1 Publication

    Protein-protein interaction databases

    BioGridi203318, 2 interactors
    STRINGi10090.ENSMUSP00000041557

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z127
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1287 Eukaryota
    COG0531 LUCA
    GeneTreeiENSGT00760000119037
    HOGENOMiHOG000098892
    HOVERGENiHBG000476
    InParanoidiQ9Z127
    KOiK13780
    OMAiHKAFGIH
    OrthoDBiEOG091G07EM
    TreeFamiTF313355

    Family and domain databases

    InterProiView protein in InterPro
    IPR002293 AA/rel_permease1
    IPR004760 L_AA_transporter
    PfamiView protein in Pfam
    PF13520 AA_permease_2, 1 hit
    PIRSFiPIRSF006060 AA_transporter, 1 hit
    TIGRFAMsiTIGR00911 2A0308, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9Z127-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MAVAGAKRRA VATPAAAAAE EERQAREKML EARRGDGADP EGEGVTLQRN
    60 70 80 90 100
    ITLLNGVAII VGTIIGSGIF VTPTGVLKEA GSPGLSLVVW AVCGVFSIVG
    110 120 130 140 150
    ALCYAELGTT ISKSGGDYAY MLEVYGSLPA FLKLWIELLI IRPSSQYIVA
    160 170 180 190 200
    LVFATYLLKP VFPTCPVPEE AAKLVACLCV LLLTAVNCYS VKAATRVQDA
    210 220 230 240 250
    FAAAKLLALA LIILLGFIQM GKDMGQGDAS NLQQKLSFEG TNLDVGNIVL
    260 270 280 290 300
    ALYSGLFAYG GWNYLNFVTE EMINPYRNLP LAIIISLPIV TLVYVLTNLA
    310 320 330 340 350
    YFTTLSTNQM LTSEAVAVDF GNYHLGVMSW IIPVFVGLSC FGSVNGSLFT
    360 370 380 390 400
    SSRLFFVGSR EGHLPSVLSM IHPQLLTPVP SLVFTCIMTL MYAFSRDIFS
    410 420 430 440 450
    IINFFSFFNW LCVALAIIGM MWLRFKKPEL ERPIKVNLAL PVFFILACLF
    460 470 480 490 500
    LIAVSFWKTP MECGIGFAII LSGLPVYFFG VWWKNKPKWI LQAIFSVTVL
    510
    CQKLMQVVPQ ET
    Length:512
    Mass (Da):55,872
    Last modified:July 27, 2011 - v2
    Checksum:i57045EC4DD9DE1A1
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti8R → M in BAA90556 (Ref. 2) Curated1
    Sequence conflicti16A → T in BAA75520 (PubMed:9915839).Curated1
    Sequence conflicti16A → T in BAA90556 (Ref. 2) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB017189 mRNA Translation: BAA75520.1
    AB023409 mRNA Translation: BAA90556.1
    BC026131 mRNA Translation: AAH26131.1
    CCDSiCCDS22730.1
    RefSeqiNP_035534.2, NM_011404.3
    UniGeneiMm.22374
    Mm.27943

    Genome annotation databases

    EnsembliENSMUST00000045557; ENSMUSP00000041557; ENSMUSG00000040010
    GeneIDi20539
    KEGGimmu:20539
    UCSCiuc009nsc.2 mouse

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB017189 mRNA Translation: BAA75520.1
    AB023409 mRNA Translation: BAA90556.1
    BC026131 mRNA Translation: AAH26131.1
    CCDSiCCDS22730.1
    RefSeqiNP_035534.2, NM_011404.3
    UniGeneiMm.22374
    Mm.27943

    3D structure databases

    ProteinModelPortaliQ9Z127
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi203318, 2 interactors
    STRINGi10090.ENSMUSP00000041557

    PTM databases

    iPTMnetiQ9Z127
    PhosphoSitePlusiQ9Z127
    SwissPalmiQ9Z127

    Proteomic databases

    EPDiQ9Z127
    MaxQBiQ9Z127
    PaxDbiQ9Z127
    PRIDEiQ9Z127

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000045557; ENSMUSP00000041557; ENSMUSG00000040010
    GeneIDi20539
    KEGGimmu:20539
    UCSCiuc009nsc.2 mouse

    Organism-specific databases

    CTDi8140
    MGIiMGI:1298205 Slc7a5

    Phylogenomic databases

    eggNOGiKOG1287 Eukaryota
    COG0531 LUCA
    GeneTreeiENSGT00760000119037
    HOGENOMiHOG000098892
    HOVERGENiHBG000476
    InParanoidiQ9Z127
    KOiK13780
    OMAiHKAFGIH
    OrthoDBiEOG091G07EM
    TreeFamiTF313355

    Enzyme and pathway databases

    ReactomeiR-MMU-210991 Basigin interactions
    R-MMU-352230 Amino acid transport across the plasma membrane
    R-MMU-71240 Tryptophan catabolism

    Miscellaneous databases

    ChiTaRSiSlc7a5 mouse
    PROiPR:Q9Z127
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000040010 Expressed in 286 organ(s), highest expression level in brain blood vessel
    GenevisibleiQ9Z127 MM

    Family and domain databases

    InterProiView protein in InterPro
    IPR002293 AA/rel_permease1
    IPR004760 L_AA_transporter
    PfamiView protein in Pfam
    PF13520 AA_permease_2, 1 hit
    PIRSFiPIRSF006060 AA_transporter, 1 hit
    TIGRFAMsiTIGR00911 2A0308, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiLAT1_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z127
    Secondary accession number(s): Q8R0X8, Q9JMI4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: July 27, 2011
    Last modified: November 7, 2018
    This is version 146 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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