UniProtKB - Q9Z110 (P5CS_MOUSE)
Protein
Delta-1-pyrroline-5-carboxylate synthase
Gene
Aldh18a1
Organism
Mus musculus (Mouse)
Status
Functioni
Bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine.By similarity
Catalytic activityi
Activity regulationi
Isoform Short: Inhibited by L-ornithine with a Ki of approximately 0.25 mm. Isoform Long: Insensitive to ornithine inhibition. Thus, the two amino acid insert in the long isoform abolishes feedback inhibition of P5CS activity by L-ornithine.1 Publication
: L-proline biosynthesis Pathwayi
This protein is involved in step 1 and 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.By similarityProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Delta-1-pyrroline-5-carboxylate synthase (Aldh18a1), Delta-1-pyrroline-5-carboxylate synthase (Aldh18a1), Gamma-glutamyl kinase (Aldh18a1)
- Delta-1-pyrroline-5-carboxylate synthase (Aldh18a1), Delta-1-pyrroline-5-carboxylate synthase (Aldh18a1), Gamma-glutamyl kinase (Aldh18a1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 117 | SubstrateBy similarity | 1 | |
Binding sitei | 223 | SubstrateBy similarity | 1 | |
Binding sitei | 246 | Substrate; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 266 – 267 | ATPBy similarity | 2 | |
Nucleotide bindingi | 305 – 311 | ATPBy similarity | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glutamate 5-kinase activity Source: MGI
- glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB
- identical protein binding Source: MGI
GO - Biological processi
- cellular amino acid biosynthetic process Source: MGI
- citrulline biosynthetic process Source: UniProtKB
- glutamate metabolic process Source: UniProtKB
- L-proline biosynthetic process Source: UniProtKB-UniPathway
- ornithine biosynthetic process Source: MGI
- proline biosynthetic process Source: UniProtKB
- response to temperature stimulus Source: MGI
Keywordsi
Molecular function | Kinase, Multifunctional enzyme, Oxidoreductase, Transferase |
Biological process | Amino-acid biosynthesis, Proline biosynthesis |
Ligand | ATP-binding, NADP, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 1.2.1.41, 3474 |
Reactomei | R-MMU-8964539, Glutamate and glutamine metabolism |
UniPathwayi | UPA00098;UER00359 UPA00098;UER00360 |
Names & Taxonomyi
Protein namesi | Recommended name: Delta-1-pyrroline-5-carboxylate synthaseShort name: P5CS Alternative name(s): Aldehyde dehydrogenase family 18 member A1 Including the following 2 domains: |
Gene namesi | Name:Aldh18a1 Synonyms:P5cs, Pycs |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1888908, Aldh18a1 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
- mitochondrial membrane Source: MGI
- mitochondrion Source: MGI
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109770 | 1 – 795 | Delta-1-pyrroline-5-carboxylate synthaseAdd BLAST | 795 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 311 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 347 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 550 | N6-succinyllysineCombined sources | 1 |
Proteomic databases
EPDi | Q9Z110 |
PaxDbi | Q9Z110 |
PeptideAtlasi | Q9Z110 |
PRIDEi | Q9Z110 |
TopDownProteomicsi | Q9Z110-1 [Q9Z110-1] |
PTM databases
iPTMneti | Q9Z110 |
PhosphoSitePlusi | Q9Z110 |
SwissPalmi | Q9Z110 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000025007, Expressed in epithelium of small intestine and 318 other tissues |
ExpressionAtlasi | Q9Z110, baseline and differential |
Genevisiblei | Q9Z110, MM |
Interactioni
Subunit structurei
Homohexamer or homotetramer.
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 207993, 3 interactors |
IntActi | Q9Z110, 7 interactors |
MINTi | Q9Z110 |
STRINGi | 10090.ENSMUSP00000025979 |
Miscellaneous databases
RNActi | Q9Z110, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 361 | Glutamate 5-kinaseAdd BLAST | 361 | |
Regioni | 362 – 795 | Gamma-glutamyl phosphate reductaseAdd BLAST | 434 |
Sequence similaritiesi
In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated
Phylogenomic databases
eggNOGi | KOG1154, Eukaryota KOG4165, Eukaryota |
GeneTreei | ENSGT00500000044903 |
HOGENOMi | CLU_016144_0_0_1 |
InParanoidi | Q9Z110 |
OMAi | EGRECIM |
OrthoDBi | 832430at2759 |
TreeFami | TF314372 |
Family and domain databases
CDDi | cd04256, AAK_P5CS_ProBA, 1 hit cd07079, ALDH_F18-19_ProA-GPR, 1 hit |
Gene3Di | 3.40.1160.10, 1 hit 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
HAMAPi | MF_00412, ProA, 1 hit MF_00456, ProB, 1 hit |
InterProi | View protein in InterPro IPR036393, AceGlu_kinase-like_sf IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR001048, Asp/Glu/Uridylate_kinase IPR020593, G-glutamylP_reductase_CS IPR041744, G5K_ProBA IPR001057, Glu/AcGlu_kinase IPR005715, Glu_5kinase/COase_Synthase IPR019797, Glutamate_5-kinase_CS IPR000965, GPR_dom IPR005766, P5_carboxy_syn |
Pfami | View protein in Pfam PF00696, AA_kinase, 1 hit PF00171, Aldedh, 1 hit |
PIRSFi | PIRSF036429, P5C_syn, 1 hit |
PRINTSi | PR00474, GLU5KINASE |
SUPFAMi | SSF53633, SSF53633, 1 hit SSF53720, SSF53720, 1 hit |
TIGRFAMsi | TIGR01092, P5CS, 1 hit TIGR00407, proA, 1 hit |
PROSITEi | View protein in PROSITE PS00902, GLUTAMATE_5_KINASE, 1 hit PS01223, PROA, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform Long (identifier: Q9Z110-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLRHMHRSGV QPFRQRLLPW VQSIAVPRSN RVQPSAIRHV RSWSNIPFIT
60 70 80 90 100
VPLSRAHGKP FAHRSELKHA KRIVVKLGSA VVTRGDECGL ALGRLASIVE
110 120 130 140 150
QVSVLQNQGR EMMLVTSGAV AFGKQRLRHE ILLSQSVRQA LHSGQNHLKE
160 170 180 190 200
MAIPVLEARA CAAAGQSGLM ALYEAMFTQY SICAAQILVT NLDFHDEQKR
210 220 230 240 250
RNLNGTLHEL LRMNIVPIVN TNDAVVPPAE PNSDLQGVNV ISVKDNDSLA
260 270 280 290 300
ARLAVEMKTD LLIVLSDVEG LFDSPPGSDD AKLIDIFYPG DQQSVTFGTK
310 320 330 340 350
SRVGLGGMEA KVKAALWALQ GGTSVVIANG THPKVSGHVI TDIVEGKKVG
360 370 380 390 400
TFFSEVKPAG PTVEQQGEMA RSGGRMLATL EPEQRAEIIN HLADLLTDQR
410 420 430 440 450
EEILLANKKD LEEAEGRLAS PLLKRLSLST SKLNSLAIGL RQIAASSQES
460 470 480 490 500
VGRVLRRTRI AKNLELEQVT VPIGVLLVIF ESRPDCLPQV AALAIASGNG
510 520 530 540 550
LLLKGGKEAA HSNRILHLLT QEALSIHGVK EAIQLVNTRE EVEDLCRLDK
560 570 580 590 600
IIDLIIPRGS SQLVRDIQKA AKGIPVMGHS EGICHMYVDS EASVDKVTRL
610 620 630 640 650
VRDSKCEYPA ACNALETLLI HRDLLRTPLF DQIIDMLRVE QVKIHAGPKF
660 670 680 690 700
ASYLTFSPSE VKSLRTEYGD LEVCIEVVDS VQEAIDHIHK YGSSHTDVIV
710 720 730 740 750
TENEKTAEFF LQHVDSACVF WNASTRFSDG YRFGLGAEVG ISTSRIHARG
760 770 780 790
PVGLEGLLTT KWLLRGQDHV VSDFSEHGSL KYLHENLPVP QRNFS
Isoform Short (identifier: Q9Z110-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
239-240: Missing.
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3Z0B4 | D3Z0B4_MOUSE | Delta-1-pyrroline-5-carboxylate syn... | Aldh18a1 | 173 | Annotation score: | ||
H3BKK0 | H3BKK0_MOUSE | Delta-1-pyrroline-5-carboxylate syn... | Aldh18a1 | 100 | Annotation score: | ||
H3BLE8 | H3BLE8_MOUSE | Delta-1-pyrroline-5-carboxylate syn... | Aldh18a1 | 83 | Annotation score: | ||
H3BKJ8 | H3BKJ8_MOUSE | Delta-1-pyrroline-5-carboxylate syn... | Aldh18a1 | 48 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 582 | G → S in AAD17517 (PubMed:10037775).Curated | 1 | |
Sequence conflicti | 582 | G → S in AAD17518 (PubMed:10037775).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005216 | 239 – 240 | Missing in isoform Short. Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF056573 mRNA Translation: AAD17517.1 AF056574 mRNA Translation: AAD17518.1 AK151072 mRNA Translation: BAE30088.1 AK153946 mRNA Translation: BAE32270.1 AK168905 mRNA Translation: BAE40719.1 CH466534 Genomic DNA Translation: EDL41837.1 BC033427 mRNA Translation: AAH33427.1 BC037699 mRNA Translation: AAH37699.1 |
CCDSi | CCDS29803.1 [Q9Z110-1] CCDS57144.1 [Q9Z110-2] |
RefSeqi | NP_062672.2, NM_019698.2 [Q9Z110-1] NP_705782.2, NM_153554.2 [Q9Z110-2] |
Genome annotation databases
Ensembli | ENSMUST00000025979; ENSMUSP00000025979; ENSMUSG00000025007 [Q9Z110-1] ENSMUST00000176939; ENSMUSP00000135426; ENSMUSG00000025007 [Q9Z110-2] |
GeneIDi | 56454 |
KEGGi | mmu:56454 |
UCSCi | uc008hkw.2, mouse [Q9Z110-2] uc008hkx.2, mouse [Q9Z110-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF056573 mRNA Translation: AAD17517.1 AF056574 mRNA Translation: AAD17518.1 AK151072 mRNA Translation: BAE30088.1 AK153946 mRNA Translation: BAE32270.1 AK168905 mRNA Translation: BAE40719.1 CH466534 Genomic DNA Translation: EDL41837.1 BC033427 mRNA Translation: AAH33427.1 BC037699 mRNA Translation: AAH37699.1 |
CCDSi | CCDS29803.1 [Q9Z110-1] CCDS57144.1 [Q9Z110-2] |
RefSeqi | NP_062672.2, NM_019698.2 [Q9Z110-1] NP_705782.2, NM_153554.2 [Q9Z110-2] |
3D structure databases
SMRi | Q9Z110 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207993, 3 interactors |
IntActi | Q9Z110, 7 interactors |
MINTi | Q9Z110 |
STRINGi | 10090.ENSMUSP00000025979 |
PTM databases
iPTMneti | Q9Z110 |
PhosphoSitePlusi | Q9Z110 |
SwissPalmi | Q9Z110 |
Proteomic databases
EPDi | Q9Z110 |
PaxDbi | Q9Z110 |
PeptideAtlasi | Q9Z110 |
PRIDEi | Q9Z110 |
TopDownProteomicsi | Q9Z110-1 [Q9Z110-1] |
Protocols and materials databases
Antibodypediai | 2043, 200 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000025979; ENSMUSP00000025979; ENSMUSG00000025007 [Q9Z110-1] ENSMUST00000176939; ENSMUSP00000135426; ENSMUSG00000025007 [Q9Z110-2] |
GeneIDi | 56454 |
KEGGi | mmu:56454 |
UCSCi | uc008hkw.2, mouse [Q9Z110-2] uc008hkx.2, mouse [Q9Z110-1] |
Organism-specific databases
CTDi | 5832 |
MGIi | MGI:1888908, Aldh18a1 |
Phylogenomic databases
eggNOGi | KOG1154, Eukaryota KOG4165, Eukaryota |
GeneTreei | ENSGT00500000044903 |
HOGENOMi | CLU_016144_0_0_1 |
InParanoidi | Q9Z110 |
OMAi | EGRECIM |
OrthoDBi | 832430at2759 |
TreeFami | TF314372 |
Enzyme and pathway databases
UniPathwayi | UPA00098;UER00359 UPA00098;UER00360 |
BRENDAi | 1.2.1.41, 3474 |
Reactomei | R-MMU-8964539, Glutamate and glutamine metabolism |
Miscellaneous databases
BioGRID-ORCSi | 56454, 1 hit in 19 CRISPR screens |
ChiTaRSi | Aldh18a1, mouse |
PROi | PR:Q9Z110 |
RNActi | Q9Z110, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025007, Expressed in epithelium of small intestine and 318 other tissues |
ExpressionAtlasi | Q9Z110, baseline and differential |
Genevisiblei | Q9Z110, MM |
Family and domain databases
CDDi | cd04256, AAK_P5CS_ProBA, 1 hit cd07079, ALDH_F18-19_ProA-GPR, 1 hit |
Gene3Di | 3.40.1160.10, 1 hit 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
HAMAPi | MF_00412, ProA, 1 hit MF_00456, ProB, 1 hit |
InterProi | View protein in InterPro IPR036393, AceGlu_kinase-like_sf IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR001048, Asp/Glu/Uridylate_kinase IPR020593, G-glutamylP_reductase_CS IPR041744, G5K_ProBA IPR001057, Glu/AcGlu_kinase IPR005715, Glu_5kinase/COase_Synthase IPR019797, Glutamate_5-kinase_CS IPR000965, GPR_dom IPR005766, P5_carboxy_syn |
Pfami | View protein in Pfam PF00696, AA_kinase, 1 hit PF00171, Aldedh, 1 hit |
PIRSFi | PIRSF036429, P5C_syn, 1 hit |
PRINTSi | PR00474, GLU5KINASE |
SUPFAMi | SSF53633, SSF53633, 1 hit SSF53720, SSF53720, 1 hit |
TIGRFAMsi | TIGR01092, P5CS, 1 hit TIGR00407, proA, 1 hit |
PROSITEi | View protein in PROSITE PS00902, GLUTAMATE_5_KINASE, 1 hit PS01223, PROA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | P5CS_MOUSE | |
Accessioni | Q9Z110Primary (citable) accession number: Q9Z110 Secondary accession number(s): Q8BGM2, Q9R1P6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 178 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families