UniProtKB - Q9Z0U1 (ZO2_MOUSE)
Protein
Tight junction protein ZO-2
Gene
Tjp2
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a role in tight junctions and adherens junctions.
GO - Molecular functioni
- cell adhesion molecule binding Source: GO_Central
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- protein-macromolecule adaptor activity Source: MGI
- protein tyrosine kinase binding Source: MGI
GO - Biological processi
- cell-cell adhesion Source: GO_Central
- cell-cell junction organization Source: GO_Central
- establishment of endothelial intestinal barrier Source: MGI
- homotypic cell-cell adhesion Source: MGI
- intestinal absorption Source: MGI
- negative regulation of osteoclast development Source: MGI
- positive regulation of blood-brain barrier permeability Source: MGI
- protein localization to cell-cell junction Source: GO_Central
- regulation of membrane permeability Source: MGI
- TNFSF11-mediated signaling pathway Source: MGI
Enzyme and pathway databases
Reactomei | R-MMU-2028269, Signaling by Hippo R-MMU-351906, Apoptotic cleavage of cell adhesion proteins |
Names & Taxonomyi
Protein namesi | Recommended name: Tight junction protein ZO-2Alternative name(s): Tight junction protein 2 Zona occludens protein 2 Zonula occludens protein 2 |
Gene namesi | Name:Tjp2 Synonyms:Zo2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1341872, Tjp2 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Nucleus
- Nucleus By similarity
Other locations
- tight junction 1 Publication
Note: Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.By similarity
Nucleus
- nucleus Source: ARUK-UCL
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- bicellular tight junction Source: MGI
- cell junction Source: MGI
- cell surface Source: MGI
- cell-cell contact zone Source: MGI
- cell-cell junction Source: ARUK-UCL
- gap junction Source: MGI
- tight junction Source: MGI
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Nucleus, Tight junctionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094544 | 1 – 1167 | Tight junction protein ZO-2Add BLAST | 1167 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 107 | PhosphoserineCombined sources | 1 | |
Modified residuei | 127 | PhosphoserineBy similarity | 1 | |
Modified residuei | 130 | PhosphoserineBy similarity | 1 | |
Modified residuei | 140 | PhosphoserineCombined sources | 1 | |
Modified residuei | 145 | PhosphoserineBy similarity | 1 | |
Modified residuei | 147 | PhosphoserineBy similarity | 1 | |
Modified residuei | 173 | PhosphoserineBy similarity | 1 | |
Modified residuei | 194 | PhosphoserineBy similarity | 1 | |
Modified residuei | 205 | PhosphoserineBy similarity | 1 | |
Modified residuei | 239 | PhosphoserineCombined sources | 1 | |
Modified residuei | 305 | PhosphoserineBy similarity | 1 | |
Modified residuei | 378 | PhosphoserineCombined sources | 1 | |
Modified residuei | 380 | PhosphoserineCombined sources | 1 | |
Modified residuei | 386 | PhosphoserineBy similarity | 1 | |
Modified residuei | 395 | PhosphoserineBy similarity | 1 | |
Modified residuei | 404 | PhosphoserineBy similarity | 1 | |
Modified residuei | 410 | PhosphoserineBy similarity | 1 | |
Modified residuei | 411 | PhosphoserineBy similarity | 1 | |
Modified residuei | 435 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 479 | PhosphoserineCombined sources | 1 | |
Modified residuei | 554 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 684 | PhosphoserineCombined sources | 1 | |
Modified residuei | 884 | PhosphoserineCombined sources | 1 | |
Modified residuei | 887 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 895 | PhosphoserineCombined sources | 1 | |
Modified residuei | 902 | PhosphoserineBy similarity | 1 | |
Modified residuei | 907 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 915 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 948 | PhosphoserineBy similarity | 1 | |
Modified residuei | 960 | PhosphoserineBy similarity | 1 | |
Modified residuei | 968 | PhosphoserineCombined sources | 1 | |
Modified residuei | 988 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1044 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1095 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 1124 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1136 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9Z0U1 |
jPOSTi | Q9Z0U1 |
MaxQBi | Q9Z0U1 |
PaxDbi | Q9Z0U1 |
PeptideAtlasi | Q9Z0U1 |
PRIDEi | Q9Z0U1 |
PTM databases
iPTMneti | Q9Z0U1 |
PhosphoSitePlusi | Q9Z0U1 |
SwissPalmi | Q9Z0U1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024812, Expressed in ear vesicle and 297 other tissues |
ExpressionAtlasi | Q9Z0U1, baseline and differential |
Genevisiblei | Q9Z0U1, MM |
Interactioni
Subunit structurei
Homodimer, and heterodimer with ZO1.
Interacts with UBN1.
Interacts with SCRIB (By similarity).
Interacts with occludin and SAFB. Interaction with SAFB occurs in the nucleus.
Interacts with USP53 (via the C-terminal region) (PubMed:26609154).
By similarity2 PublicationsGO - Molecular functioni
- cell adhesion molecule binding Source: GO_Central
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- protein tyrosine kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204210, 5 interactors |
IntActi | Q9Z0U1, 4 interactors |
MINTi | Q9Z0U1 |
STRINGi | 10090.ENSMUSP00000097154 |
Miscellaneous databases
RNActi | Q9Z0U1, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9Z0U1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9Z0U1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 97 | PDZ 1PROSITE-ProRule annotationAdd BLAST | 88 | |
Domaini | 287 – 365 | PDZ 2PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 489 – 570 | PDZ 3PROSITE-ProRule annotationAdd BLAST | 82 | |
Domaini | 584 – 649 | SH3PROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 660 – 858 | Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST | 199 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1165 – 1167 | Interaction with SCRIBBy similarity | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1139 – 1142 | Poly-Glu | 4 |
Sequence similaritiesi
Belongs to the MAGUK family.Curated
Keywords - Domaini
Repeat, SH3 domainPhylogenomic databases
eggNOGi | KOG3580, Eukaryota |
GeneTreei | ENSGT00940000158634 |
HOGENOMi | CLU_006234_1_0_1 |
InParanoidi | Q9Z0U1 |
OMAi | KMDHKAK |
OrthoDBi | 1175136at2759 |
PhylomeDBi | Q9Z0U1 |
TreeFami | TF315957 |
Family and domain databases
CDDi | cd12027, SH3_ZO-2, 1 hit |
Gene3Di | 2.30.42.10, 3 hits |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR027417, P-loop_NTPase IPR001478, PDZ IPR036034, PDZ_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR005417, ZO IPR005419, ZO-2 IPR035598, ZO-2_SH3 |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF00595, PDZ, 3 hits PF07653, SH3_2, 1 hit |
PRINTSi | PR01597, ZONOCCLUDNS PR01599, ZONOCCLUDNS2 |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit SM00228, PDZ, 3 hits |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF50156, SSF50156, 3 hits SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50052, GUANYLATE_KINASE_2, 1 hit PS50106, PDZ, 3 hits PS50002, SH3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q9Z0U1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEEVIWEQYT VTLQKDSKRG FGIAVSGGRD NPHFENGETS IVISDVLPGG
60 70 80 90 100
PADGLLQEND RVVMVNGTPM EDVLHSFAVQ QLRKSGKIAA IVVKRPRKVQ
110 120 130 140 150
VAPLQGSPPL SHDDRGFEVI EEFDGRSFRS GYSERSRHSS HDMLSHSWEG
160 170 180 190 200
NRERGRPHQR TQSRERERSR GRSLERGLDQ EDYGRSRERS RGRSLERGLD
210 220 230 240 250
RDFVSRDHSR GRSIDRDYDR DYERSYHEAY EPDYGGGYSP SYDRRAHPET
260 270 280 290 300
RYERSRSREH LRSRSPSPES RSRHEHKGQH DPDRPIGVLL TKSKANEEYG
310 320 330 340 350
LRLGSQIFIK EMTRTGLATK DGNLHEGDII LKINGTVTEN MSLTDARKLI
360 370 380 390 400
EKSRGKLQLV VLRDSKQTLI NIPALNDSDS EVEDISEIES NRSFSPEERR
410 420 430 440 450
QQYSDQDYHS STEKLKERPS SREETSGRLS RMGATPTPFK STGDITAAGV
460 470 480 490 500
TEASREPRYQ EEGPVPQPRT APRVFLRPSP EDEAIYGPNT KMVRFKKGDS
510 520 530 540 550
VGLRLAGGND VGIFVAGIQE GTSAEQEGLQ EGDQILKVNT QDFRGLVRED
560 570 580 590 600
AVLYLLEIPK GETVTILAQS RADVYRDILA CGRGDSFFIR SHFECEKETP
610 620 630 640 650
QSLAFTRGEV FRVVDTLYDG KLGHWLAVRI GNELEKGLIP NKSRAEQMAS
660 670 680 690 700
VQNAQRENAG DRADFWRMRG QRSSGGVKKN LRKSREDLAA AVSVSTKFPA
710 720 730 740 750
YEKVLLREAG FKRPVVLFGP IADIAMERLA TELPDLFQTA KTEPKDAGSE
760 770 780 790 800
KSSGVVRLNT VRQIIEQDKH ALLDVTPKAV DLLNYTQWFP IVIFFNPDSR
810 820 830 840 850
QGVKTIRQRL SPTSNKSSRK LFDQANKLKK TCSHLFTATI NVNSANDGWF
860 870 880 890 900
GSLKDSIQQQ QNEAVWVSEG KMEGMDDDAE DRMSYLTAMG ADYLSCDSRL
910 920 930 940 950
ISDFEDTDGE GGAYTDNELE EPAEEPLVSS ITRSSEPVQH EENIRKSSPE
960 970 980 990 1000
PRAQMRRAAS RDQLRDASPP PAFKPEPPKA RSQNREDSFD YSKSNLPATA
1010 1020 1030 1040 1050
GSEIPGGSTK GYPPPIAAKP AFGRPILKPS TPVPMPESEE VGESTEEQED
1060 1070 1080 1090 1100
APRSVLGRVK IFEKMDHKAK LQRMQELQEA QNARIEIAQK HPDIYAVPIK
1110 1120 1130 1140 1150
APKPDAGLPP HMSSRPPEPQ KAPSRLYQDT RGSYGSDPEE EEYRQQLAAH
1160
SKRGYYSQPS RYRDTEL
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3B2WBH9 | A0A3B2WBH9_MOUSE | Tight junction protein ZO-2 | Tjp2 | 1,165 | Annotation score: | ||
A0A3B2WCN9 | A0A3B2WCN9_MOUSE | Tight junction protein ZO-2 | Tjp2 | 1,190 | Annotation score: | ||
A0A494B9E6 | A0A494B9E6_MOUSE | Tight junction protein ZO-2 | Tjp2 | 171 | Annotation score: | ||
A0A494BAA0 | A0A494BAA0_MOUSE | Tight junction protein ZO-2 | Tjp2 | 53 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 100 | Q → L in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 105 – 107 | QGS → LGC in AAD19964 (PubMed:10026224).Curated | 3 | |
Sequence conflicti | 111 – 112 | SH → CL in AAD19964 (PubMed:10026224).Curated | 2 | |
Sequence conflicti | 156 | R → G in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 292 | K → Q in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 378 | S → R in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 447 | A → P in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 454 | S → T in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 470 | T → A in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 506 | A → P in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 633 | E → D in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 649 | A → D in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 664 | D → V in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 667 | R → W in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 670 | G → R in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 674 | S → R in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 677 | V → D in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 680 | N → T in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 690 – 691 | AA → RS in AAD19964 (PubMed:10026224).Curated | 2 | |
Sequence conflicti | 731 | T → N in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 1035 | M → I in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 1038 | S → T in AAD19964 (PubMed:10026224).Curated | 1 | |
Sequence conflicti | 1069 | A → P in AAD19964 (PubMed:10026224).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF113005 mRNA Translation: AAD19964.1 BC034677 mRNA Translation: AAH34677.1 BC039924 mRNA Translation: AAH39924.1 |
CCDSi | CCDS89365.1 |
RefSeqi | NP_035727.2, NM_011597.4 XP_006526971.1, XM_006526908.3 XP_011245521.1, XM_011247219.2 |
Genome annotation databases
Ensembli | ENSMUST00000099558; ENSMUSP00000097154; ENSMUSG00000024812 ENSMUST00000233658; ENSMUSP00000156728; ENSMUSG00000024812 |
GeneIDi | 21873 |
KEGGi | mmu:21873 |
UCSCi | uc008ham.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF113005 mRNA Translation: AAD19964.1 BC034677 mRNA Translation: AAH34677.1 BC039924 mRNA Translation: AAH39924.1 |
CCDSi | CCDS89365.1 |
RefSeqi | NP_035727.2, NM_011597.4 XP_006526971.1, XM_006526908.3 XP_011245521.1, XM_011247219.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CSJ | NMR | - | A | 1-104 | [»] | |
SMRi | Q9Z0U1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204210, 5 interactors |
IntActi | Q9Z0U1, 4 interactors |
MINTi | Q9Z0U1 |
STRINGi | 10090.ENSMUSP00000097154 |
PTM databases
iPTMneti | Q9Z0U1 |
PhosphoSitePlusi | Q9Z0U1 |
SwissPalmi | Q9Z0U1 |
Proteomic databases
EPDi | Q9Z0U1 |
jPOSTi | Q9Z0U1 |
MaxQBi | Q9Z0U1 |
PaxDbi | Q9Z0U1 |
PeptideAtlasi | Q9Z0U1 |
PRIDEi | Q9Z0U1 |
Genome annotation databases
Ensembli | ENSMUST00000099558; ENSMUSP00000097154; ENSMUSG00000024812 ENSMUST00000233658; ENSMUSP00000156728; ENSMUSG00000024812 |
GeneIDi | 21873 |
KEGGi | mmu:21873 |
UCSCi | uc008ham.2, mouse |
Organism-specific databases
CTDi | 9414 |
MGIi | MGI:1341872, Tjp2 |
Phylogenomic databases
eggNOGi | KOG3580, Eukaryota |
GeneTreei | ENSGT00940000158634 |
HOGENOMi | CLU_006234_1_0_1 |
InParanoidi | Q9Z0U1 |
OMAi | KMDHKAK |
OrthoDBi | 1175136at2759 |
PhylomeDBi | Q9Z0U1 |
TreeFami | TF315957 |
Enzyme and pathway databases
Reactomei | R-MMU-2028269, Signaling by Hippo R-MMU-351906, Apoptotic cleavage of cell adhesion proteins |
Miscellaneous databases
BioGRID-ORCSi | 21873, 1 hit in 17 CRISPR screens |
ChiTaRSi | Tjp2, mouse |
EvolutionaryTracei | Q9Z0U1 |
PROi | PR:Q9Z0U1 |
RNActi | Q9Z0U1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024812, Expressed in ear vesicle and 297 other tissues |
ExpressionAtlasi | Q9Z0U1, baseline and differential |
Genevisiblei | Q9Z0U1, MM |
Family and domain databases
CDDi | cd12027, SH3_ZO-2, 1 hit |
Gene3Di | 2.30.42.10, 3 hits |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR027417, P-loop_NTPase IPR001478, PDZ IPR036034, PDZ_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR005417, ZO IPR005419, ZO-2 IPR035598, ZO-2_SH3 |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF00595, PDZ, 3 hits PF07653, SH3_2, 1 hit |
PRINTSi | PR01597, ZONOCCLUDNS PR01599, ZONOCCLUDNS2 |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit SM00228, PDZ, 3 hits |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF50156, SSF50156, 3 hits SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50052, GUANYLATE_KINASE_2, 1 hit PS50106, PDZ, 3 hits PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZO2_MOUSE | |
Accessioni | Q9Z0U1Primary (citable) accession number: Q9Z0U1 Secondary accession number(s): Q8K210 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | May 2, 2006 | |
Last modified: | December 2, 2020 | |
This is version 190 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families