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Protein

Claudin-10

Gene

Cldn10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Involved in the regulation of paracellular epithelia permeability to ions in multiple organs. It acts as a paracellular ion channel probably forming permselective pores; isoform 1 appears to create pores preferentially permeable to cations and isoform 2 for anions. In sweat glands and in the thick ascending limb (TAL) of Henle's loop in kidney, it controls paracellular sodium permeability which is essential for proper sweat production and renal function.By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processIon transport, Transport

Protein family/group databases

TCDBi1.H.1.1.10 the claudin tight junction (claudin1) family

Names & Taxonomyi

Protein namesi
Recommended name:
Claudin-10
Gene namesi
Name:Cldn10
Synonyms:Cldn10a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1913101 Cldn10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21
Topological domaini22 – 80ExtracellularSequence analysisAdd BLAST59
Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
Topological domaini102 – 115CytoplasmicSequence analysisAdd BLAST14
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Topological domaini137 – 160ExtracellularSequence analysisAdd BLAST24
Transmembranei161 – 181HelicalSequence analysisAdd BLAST21
Topological domaini182 – 231CytoplasmicSequence analysisAdd BLAST50

Keywords - Cellular componenti

Cell junction, Cell membrane, Endoplasmic reticulum, Membrane, Tight junction

Pathology & Biotechi

Disruption phenotypei

Conditional knockout in the thick ascending limb (TAL) of Henle's loop in kidney leads to hypocalcemia, hypermagnesemia, hyperphosphatemia and nephrocalcinosis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001447581 – 231Claudin-10Add BLAST231

Proteomic databases

PaxDbiQ9Z0S6
PRIDEiQ9Z0S6

PTM databases

iPTMnetiQ9Z0S6
PhosphoSitePlusiQ9Z0S6

Expressioni

Tissue specificityi

Strong expression detected in brain cortex and kidney and weak expression in lung and cecum. In kidney, detected in thick ascending limb (TAL) of Henle's loop and proximal convoluted tubule (PCT). Isoform 1 is widely expressed, with highest expression detected in brain cortex, kidney and lung. Isoform 2, isoform 3, isoform 4 and isoform 5 are only detected in kidney and uterus. In kidney, the expression of isoform 1 is highest in medulla, with transcripts being detected in medullary thick ascending limb of Henle's loop (mTAL) and outer and inner medullary collecting ducts, whereas isoform 2 (along with isoform 4) is more highly expressed in cortex, with transcripts being detected in PCT, mTAL and cortical collecting duct. Expressed in the inner ear where it is detected in organ of Corti, marginal cells of stria vascularis, Reissner's membrane and spiral limbus (at protein level) (PubMed:14698084). Expressed in salivary glands and skin (PubMed:28771254).5 Publications

Developmental stagei

Detected in developing kidney at E14, with levels increasing towards adulthood. Expressed during tooth development: at E12, detected in the thickening tooth epithelium, at E13.5 in the lingual basal epithelium of the bud epithelium, at E14.5 in lingual epithelium and between E18 to postnatal day 0 in odontoblasts and stratum intermedium.2 Publications

Gene expression databases

BgeeiENSMUSG00000022132 Expressed in 176 organ(s), highest expression level in submandibular gland
GenevisibleiQ9Z0S6 MM

Interactioni

Subunit structurei

Can form homodimers both in trans (interaction between CLDN10 molecules in opposing membranes) and in cis (interaction between CLDN10 molecules within one membrane).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CLDN19Q8N6F1-22EBI-15799971,EBI-12256978From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-48952N
DIP-48954N
IntActiQ9Z0S6, 4 interactors
STRINGi10090.ENSMUSP00000097889

Structurei

3D structure databases

ProteinModelPortaliQ9Z0S6
SMRiQ9Z0S6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The fourth transmembrane region (161-181), which is missing in isoform 3, isoform 5 and isoform 6, is necessary for integration into tight junctions.1 Publication

Sequence similaritiesi

Belongs to the claudin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQ7E Eukaryota
ENOG410YF7U LUCA
GeneTreeiENSGT00760000119222
HOGENOMiHOG000220937
HOVERGENiHBG000643
InParanoidiQ9Z0S6
KOiK06087
OMAiGYAYNGA
OrthoDBiEOG091G0MX2
TreeFamiTF331936

Family and domain databases

InterProiView protein in InterPro
IPR006187 Claudin
IPR003554 Claudin10
IPR017974 Claudin_CS
IPR004031 PMP22/EMP/MP20/Claudin
PANTHERiPTHR12002 PTHR12002, 1 hit
PTHR12002:SF13 PTHR12002:SF13, 1 hit
PfamiView protein in Pfam
PF00822 PMP22_Claudin, 1 hit
PRINTSiPR01383 CLAUDIN10
PROSITEiView protein in PROSITE
PS01346 CLAUDIN, 1 hit

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9Z0S6-1) [UniParc]FASTAAdd to basket
Also known as: Cldn10b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASTALEIVA FVVSISGWVL VSSTLPTDYW KVSTIDGTVI TTATYFANLW
60 70 80 90 100
KICVTDSTGV ANCKEFPSML ALDGYIQACR GLMIAAVSLG FFGSIFALFG
110 120 130 140 150
MKCTKVGGSD QAKAKIACLA GIVFILSGLC SMTGCSLYAN KITTEFFDPL
160 170 180 190 200
YMEQKYELGA ALFIGWAGAS LCIIGGVIFC FSISDNNKTP RMGYTYNGPT
210 220 230
SAMSSRTKYQ GGEGDFKTTG PSKQFDKNAY V
Length:231
Mass (Da):24,695
Last modified:January 22, 2014 - v2
Checksum:i80A28863B258DED2
GO
Isoform 2 (identifier: Q9Z0S6-2) [UniParc]FASTAAdd to basket
Also known as: Cldn10a

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: MASTALEIVA...KEFPSMLALD → MSRAQISALV...RPHFTIFKVE

Show »
Length:229
Mass (Da):24,451
Checksum:i3FF402779730858E
GO
Isoform 3 (identifier: Q9Z0S6-3) [UniParc]FASTAAdd to basket
Also known as: Cldn10a_i2

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: MASTALEIVA...KEFPSMLALD → MSRAQISALV...RPHFTIFKVE
     156-191: Missing.

Show »
Length:193
Mass (Da):20,664
Checksum:iA55E86F351B7AE8C
GO
Isoform 4 (identifier: Q9Z0S6-4) [UniParc]FASTAAdd to basket
Also known as: Cldn10a_i1

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: MASTALEIVA...KEFPSMLALD → MSRAQISALV...VYQGLWMNCA

Note: Produced by alternative splicing of isoform 2.
Show »
Length:210
Mass (Da):22,309
Checksum:i8BB45CC596FD9D3D
GO
Isoform 5 (identifier: Q9Z0S6-5) [UniParc]FASTAAdd to basket
Also known as: Cldn10a_i3

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: MASTALEIVA...KEFPSMLALD → MSRAQISALV...VYQGLWMNCA
     156-191: Missing.

Note: Produced by alternative splicing of isoform 3.
Show »
Length:174
Mass (Da):18,521
Checksum:i597B914F3B16D98E
GO
Isoform 6 (identifier: Q9Z0S6-6) [UniParc]FASTAAdd to basket
Also known as: Cldn10b_i1

The sequence of this isoform differs from the canonical sequence as follows:
     156-191: Missing.

Show »
Length:195
Mass (Da):20,907
Checksum:i1103AEA5508A6DF7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104T → S in BAB32005 (Ref. 1) Curated1
Sequence conflicti202A → V in AAD17320 (Ref. 1) Curated1
Sequence conflicti202A → V in AAH21770 (PubMed:15489334).Curated1
Sequence conflicti202A → V in AAH29019 (PubMed:15489334).Curated1
Sequence conflicti219T → A in AAD17320 (Ref. 1) Curated1
Sequence conflicti219T → A in AAH21770 (PubMed:15489334).Curated1
Sequence conflicti219T → A in AAH29019 (PubMed:15489334).Curated1
Sequence conflicti219T → A no nucleotide entry (PubMed:19383724).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0535511 – 73MASTA…MLALD → MSRAQISALVCGVGGFGALV AATTSNEWKVTTRASSVITA TWVYQGLWMNCAGNALGSFH CRPHFTIFKVE in isoform 2 and isoform 3. 4 PublicationsAdd BLAST73
Alternative sequenceiVSP_0535521 – 73MASTA…MLALD → MSRAQISALVCGVGGFGALV AATTSNEWKVTTRASSVITA TWVYQGLWMNCA in isoform 4 and isoform 5. 1 PublicationAdd BLAST73
Alternative sequenceiVSP_053553156 – 191Missing in isoform 3, isoform 5 and isoform 6. 2 PublicationsAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124425 mRNA Translation: AAD17320.1
AK020131 mRNA Translation: BAB32005.1
AC154377 Genomic DNA No translation available.
CT025524 Genomic DNA No translation available.
BC021770 mRNA Translation: AAH21770.1
BC029019 mRNA Translation: AAH29019.1
CCDSiCCDS37009.1 [Q9Z0S6-2]
CCDS37010.1 [Q9Z0S6-1]
CCDS49566.1 [Q9Z0S6-3]
RefSeqiNP_001153568.1, NM_001160096.1 [Q9Z0S6-4]
NP_001153569.1, NM_001160097.1 [Q9Z0S6-3]
NP_001153570.1, NM_001160098.1 [Q9Z0S6-5]
NP_001153571.1, NM_001160099.1 [Q9Z0S6-6]
NP_067361.2, NM_021386.4 [Q9Z0S6-1]
NP_076367.2, NM_023878.3 [Q9Z0S6-2]
UniGeneiMm.390755

Genome annotation databases

EnsembliENSMUST00000047761; ENSMUSP00000041616; ENSMUSG00000022132 [Q9Z0S6-2]
ENSMUST00000071546; ENSMUSP00000071476; ENSMUSG00000022132 [Q9Z0S6-3]
ENSMUST00000100314; ENSMUSP00000097889; ENSMUSG00000022132 [Q9Z0S6-1]
GeneIDi58187
KEGGimmu:58187
UCSCiuc007uyw.2 mouse [Q9Z0S6-2]
uc007uyx.2 mouse [Q9Z0S6-1]
uc011zqb.1 mouse [Q9Z0S6-4]
uc011zqc.1 mouse [Q9Z0S6-5]
uc011zqd.1 mouse [Q9Z0S6-3]
uc011zqe.1 mouse [Q9Z0S6-6]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124425 mRNA Translation: AAD17320.1
AK020131 mRNA Translation: BAB32005.1
AC154377 Genomic DNA No translation available.
CT025524 Genomic DNA No translation available.
BC021770 mRNA Translation: AAH21770.1
BC029019 mRNA Translation: AAH29019.1
CCDSiCCDS37009.1 [Q9Z0S6-2]
CCDS37010.1 [Q9Z0S6-1]
CCDS49566.1 [Q9Z0S6-3]
RefSeqiNP_001153568.1, NM_001160096.1 [Q9Z0S6-4]
NP_001153569.1, NM_001160097.1 [Q9Z0S6-3]
NP_001153570.1, NM_001160098.1 [Q9Z0S6-5]
NP_001153571.1, NM_001160099.1 [Q9Z0S6-6]
NP_067361.2, NM_021386.4 [Q9Z0S6-1]
NP_076367.2, NM_023878.3 [Q9Z0S6-2]
UniGeneiMm.390755

3D structure databases

ProteinModelPortaliQ9Z0S6
SMRiQ9Z0S6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48952N
DIP-48954N
IntActiQ9Z0S6, 4 interactors
STRINGi10090.ENSMUSP00000097889

Protein family/group databases

TCDBi1.H.1.1.10 the claudin tight junction (claudin1) family

PTM databases

iPTMnetiQ9Z0S6
PhosphoSitePlusiQ9Z0S6

Proteomic databases

PaxDbiQ9Z0S6
PRIDEiQ9Z0S6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047761; ENSMUSP00000041616; ENSMUSG00000022132 [Q9Z0S6-2]
ENSMUST00000071546; ENSMUSP00000071476; ENSMUSG00000022132 [Q9Z0S6-3]
ENSMUST00000100314; ENSMUSP00000097889; ENSMUSG00000022132 [Q9Z0S6-1]
GeneIDi58187
KEGGimmu:58187
UCSCiuc007uyw.2 mouse [Q9Z0S6-2]
uc007uyx.2 mouse [Q9Z0S6-1]
uc011zqb.1 mouse [Q9Z0S6-4]
uc011zqc.1 mouse [Q9Z0S6-5]
uc011zqd.1 mouse [Q9Z0S6-3]
uc011zqe.1 mouse [Q9Z0S6-6]

Organism-specific databases

CTDi9071
MGIiMGI:1913101 Cldn10

Phylogenomic databases

eggNOGiENOG410IQ7E Eukaryota
ENOG410YF7U LUCA
GeneTreeiENSGT00760000119222
HOGENOMiHOG000220937
HOVERGENiHBG000643
InParanoidiQ9Z0S6
KOiK06087
OMAiGYAYNGA
OrthoDBiEOG091G0MX2
TreeFamiTF331936

Miscellaneous databases

PROiPR:Q9Z0S6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022132 Expressed in 176 organ(s), highest expression level in submandibular gland
GenevisibleiQ9Z0S6 MM

Family and domain databases

InterProiView protein in InterPro
IPR006187 Claudin
IPR003554 Claudin10
IPR017974 Claudin_CS
IPR004031 PMP22/EMP/MP20/Claudin
PANTHERiPTHR12002 PTHR12002, 1 hit
PTHR12002:SF13 PTHR12002:SF13, 1 hit
PfamiView protein in Pfam
PF00822 PMP22_Claudin, 1 hit
PRINTSiPR01383 CLAUDIN10
PROSITEiView protein in PROSITE
PS01346 CLAUDIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCLD10_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0S6
Secondary accession number(s): E9PVC8
, E9PWP4, E9QMP1, Q8VC62, Q9CX57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 22, 2014
Last modified: September 12, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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