UniProtKB - Q9Z0S6 (CLD10_MOUSE)
Protein
Claudin-10
Gene
Cldn10
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Involved in the regulation of paracellular epithelia permeability to ions in multiple organs. It acts as a paracellular ion channel probably forming permselective pores; isoform 1 appears to create pores preferentially permeable to cations and isoform 2 for anions. In sweat glands and in the thick ascending limb (TAL) of Henle's loop in kidney, it controls paracellular sodium permeability which is essential for proper sweat production and renal function.By similarity3 Publications
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- structural molecule activity Source: InterPro
GO - Biological processi
- bicellular tight junction assembly Source: GO_Central
- calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
- cell adhesion Source: GO_Central
- ion transport Source: UniProtKB-KW
- regulation of ion transport Source: UniProtKB
Keywordsi
Biological process | Ion transport, Transport |
Protein family/group databases
TCDBi | 1.H.1.1.10, the claudin tight junction (claudin1) family |
Names & Taxonomyi
Protein namesi | Recommended name: Claudin-10 |
Gene namesi | Name:Cldn10 Synonyms:Cldn10a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913101, Cldn10 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Other locations
- tight junction 2 Publications
Note: Isoform 1, isoform 2 and isoform 4 localize in the cell membrane and at epithelial tight junctions, whereas isoform 3, isoform 5 and isoform 6 are detected in the endoplasmic reticulum.1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- bicellular tight junction Source: MGI
- cytoplasm Source: MGI
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1 – 21 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 22 – 80 | ExtracellularSequence analysisAdd BLAST | 59 | |
Transmembranei | 81 – 101 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 102 – 115 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 116 – 136 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 137 – 160 | ExtracellularSequence analysisAdd BLAST | 24 | |
Transmembranei | 161 – 181 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 182 – 231 | CytoplasmicSequence analysisAdd BLAST | 50 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Endoplasmic reticulum, Membrane, Tight junctionPathology & Biotechi
Disruption phenotypei
Conditional knockout in the thick ascending limb (TAL) of Henle's loop in kidney leads to hypocalcemia, hypermagnesemia, hyperphosphatemia and nephrocalcinosis.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144758 | 1 – 231 | Claudin-10Add BLAST | 231 |
Proteomic databases
PaxDbi | Q9Z0S6 |
PRIDEi | Q9Z0S6 |
PTM databases
iPTMneti | Q9Z0S6 |
PhosphoSitePlusi | Q9Z0S6 |
Expressioni
Tissue specificityi
Strong expression detected in brain cortex and kidney and weak expression in lung and cecum. In kidney, detected in thick ascending limb (TAL) of Henle's loop and proximal convoluted tubule (PCT). Isoform 1 is widely expressed, with highest expression detected in brain cortex, kidney and lung. Isoform 2, isoform 3, isoform 4 and isoform 5 are only detected in kidney and uterus. In kidney, the expression of isoform 1 is highest in medulla, with transcripts being detected in medullary thick ascending limb of Henle's loop (mTAL) and outer and inner medullary collecting ducts, whereas isoform 2 (along with isoform 4) is more highly expressed in cortex, with transcripts being detected in PCT, mTAL and cortical collecting duct. Expressed in the inner ear where it is detected in organ of Corti, marginal cells of stria vascularis, Reissner's membrane and spiral limbus (at protein level) (PubMed:14698084). Expressed in salivary glands and skin (PubMed:28771254).5 Publications
Developmental stagei
Detected in developing kidney at 14 dpc, with levels increasing towards adulthood. Expressed during tooth development: at 12 dpc, detected in the thickening tooth epithelium, at 13.5 dpc in the lingual basal epithelium of the bud epithelium, at 14.5 dpc in lingual epithelium and between 18 dpc to postnatal day 1 in odontoblasts and stratum intermedium.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000022132, Expressed in submandibular gland and 200 other tissues |
Genevisiblei | Q9Z0S6, MM |
Interactioni
Subunit structurei
Can form homodimers both in trans (interaction between CLDN10 molecules in opposing membranes) and in cis (interaction between CLDN10 molecules within one membrane).
By similarityBinary interactionsi
Q9Z0S6
With | #Exp. | IntAct |
---|---|---|
CLDN19 - isoform 2 [Q8N6F1-2] from Homo sapiens. | 2 | EBI-15799971,EBI-12256978 |
GO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
DIPi | DIP-48952N DIP-48954N |
IntActi | Q9Z0S6, 4 interactors |
STRINGi | 10090.ENSMUSP00000097889 |
Miscellaneous databases
RNActi | Q9Z0S6, protein |
Family & Domainsi
Domaini
The fourth transmembrane region (161-181), which is missing in isoform 3, isoform 5 and isoform 6, is necessary for integration into tight junctions.1 Publication
Sequence similaritiesi
Belongs to the claudin family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QPNP, Eukaryota |
GeneTreei | ENSGT00940000155232 |
HOGENOMi | CLU_076370_0_0_1 |
InParanoidi | Q9Z0S6 |
OMAi | WILESST |
OrthoDBi | 1164514at2759 |
TreeFami | TF331936 |
Family and domain databases
InterProi | View protein in InterPro IPR006187, Claudin IPR003554, Claudin10 IPR017974, Claudin_CS IPR004031, PMP22/EMP/MP20/Claudin |
PANTHERi | PTHR12002, PTHR12002, 1 hit PTHR12002:SF13, PTHR12002:SF13, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01383, CLAUDIN10 |
PROSITEi | View protein in PROSITE PS01346, CLAUDIN, 1 hit |
s (6)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9Z0S6-1) [UniParc]FASTAAdd to basket
Also known as: Cldn10b
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASTALEIVA FVVSISGWVL VSSTLPTDYW KVSTIDGTVI TTATYFANLW
60 70 80 90 100
KICVTDSTGV ANCKEFPSML ALDGYIQACR GLMIAAVSLG FFGSIFALFG
110 120 130 140 150
MKCTKVGGSD QAKAKIACLA GIVFILSGLC SMTGCSLYAN KITTEFFDPL
160 170 180 190 200
YMEQKYELGA ALFIGWAGAS LCIIGGVIFC FSISDNNKTP RMGYTYNGPT
210 220 230
SAMSSRTKYQ GGEGDFKTTG PSKQFDKNAY V
Isoform 5 (identifier: Q9Z0S6-5) [UniParc]FASTAAdd to basket
Also known as: Cldn10a_i3
The sequence of this isoform differs from the canonical sequence as follows:
1-73: MASTALEIVA...KEFPSMLALD → MSRAQISALV...VYQGLWMNCA
156-191: Missing.
Note: Produced by alternative splicing of isoform 3.Curated
Show »Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 104 | T → S in BAB32005 (Ref. 1) Curated | 1 | |
Sequence conflicti | 202 | A → V in AAD17320 (Ref. 1) Curated | 1 | |
Sequence conflicti | 202 | A → V in AAH21770 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 202 | A → V in AAH29019 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 219 | T → A in AAD17320 (Ref. 1) Curated | 1 | |
Sequence conflicti | 219 | T → A in AAH21770 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 219 | T → A in AAH29019 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 219 | T → A no nucleotide entry (PubMed:19383724).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053551 | 1 – 73 | MASTA…MLALD → MSRAQISALVCGVGGFGALV AATTSNEWKVTTRASSVITA TWVYQGLWMNCAGNALGSFH CRPHFTIFKVE in isoform 2 and isoform 3. 4 PublicationsAdd BLAST | 73 | |
Alternative sequenceiVSP_053552 | 1 – 73 | MASTA…MLALD → MSRAQISALVCGVGGFGALV AATTSNEWKVTTRASSVITA TWVYQGLWMNCA in isoform 4 and isoform 5. 1 PublicationAdd BLAST | 73 | |
Alternative sequenceiVSP_053553 | 156 – 191 | Missing in isoform 3, isoform 5 and isoform 6. 2 PublicationsAdd BLAST | 36 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF124425 mRNA Translation: AAD17320.1 AK020131 mRNA Translation: BAB32005.1 AC154377 Genomic DNA No translation available. CT025524 Genomic DNA No translation available. BC021770 mRNA Translation: AAH21770.1 BC029019 mRNA Translation: AAH29019.1 |
CCDSi | CCDS37009.1 [Q9Z0S6-2] CCDS37010.1 [Q9Z0S6-1] CCDS49566.1 [Q9Z0S6-3] |
RefSeqi | NP_001153568.1, NM_001160096.1 [Q9Z0S6-4] NP_001153569.1, NM_001160097.1 [Q9Z0S6-3] NP_001153570.1, NM_001160098.1 [Q9Z0S6-5] NP_001153571.1, NM_001160099.1 [Q9Z0S6-6] NP_067361.2, NM_021386.4 [Q9Z0S6-1] NP_076367.2, NM_023878.3 [Q9Z0S6-2] |
Genome annotation databases
Ensembli | ENSMUST00000047761; ENSMUSP00000041616; ENSMUSG00000022132 [Q9Z0S6-2] ENSMUST00000071546; ENSMUSP00000071476; ENSMUSG00000022132 [Q9Z0S6-3] ENSMUST00000100314; ENSMUSP00000097889; ENSMUSG00000022132 [Q9Z0S6-1] |
GeneIDi | 58187 |
KEGGi | mmu:58187 |
UCSCi | uc007uyw.2, mouse [Q9Z0S6-2] uc007uyx.2, mouse [Q9Z0S6-1] uc011zqb.1, mouse [Q9Z0S6-4] uc011zqc.1, mouse [Q9Z0S6-5] uc011zqd.1, mouse [Q9Z0S6-3] uc011zqe.1, mouse [Q9Z0S6-6] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF124425 mRNA Translation: AAD17320.1 AK020131 mRNA Translation: BAB32005.1 AC154377 Genomic DNA No translation available. CT025524 Genomic DNA No translation available. BC021770 mRNA Translation: AAH21770.1 BC029019 mRNA Translation: AAH29019.1 |
CCDSi | CCDS37009.1 [Q9Z0S6-2] CCDS37010.1 [Q9Z0S6-1] CCDS49566.1 [Q9Z0S6-3] |
RefSeqi | NP_001153568.1, NM_001160096.1 [Q9Z0S6-4] NP_001153569.1, NM_001160097.1 [Q9Z0S6-3] NP_001153570.1, NM_001160098.1 [Q9Z0S6-5] NP_001153571.1, NM_001160099.1 [Q9Z0S6-6] NP_067361.2, NM_021386.4 [Q9Z0S6-1] NP_076367.2, NM_023878.3 [Q9Z0S6-2] |
3D structure databases
SMRi | Q9Z0S6 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-48952N DIP-48954N |
IntActi | Q9Z0S6, 4 interactors |
STRINGi | 10090.ENSMUSP00000097889 |
Protein family/group databases
TCDBi | 1.H.1.1.10, the claudin tight junction (claudin1) family |
PTM databases
iPTMneti | Q9Z0S6 |
PhosphoSitePlusi | Q9Z0S6 |
Proteomic databases
PaxDbi | Q9Z0S6 |
PRIDEi | Q9Z0S6 |
Protocols and materials databases
Antibodypediai | 4562, 256 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000047761; ENSMUSP00000041616; ENSMUSG00000022132 [Q9Z0S6-2] ENSMUST00000071546; ENSMUSP00000071476; ENSMUSG00000022132 [Q9Z0S6-3] ENSMUST00000100314; ENSMUSP00000097889; ENSMUSG00000022132 [Q9Z0S6-1] |
GeneIDi | 58187 |
KEGGi | mmu:58187 |
UCSCi | uc007uyw.2, mouse [Q9Z0S6-2] uc007uyx.2, mouse [Q9Z0S6-1] uc011zqb.1, mouse [Q9Z0S6-4] uc011zqc.1, mouse [Q9Z0S6-5] uc011zqd.1, mouse [Q9Z0S6-3] uc011zqe.1, mouse [Q9Z0S6-6] |
Organism-specific databases
CTDi | 9071 |
MGIi | MGI:1913101, Cldn10 |
Phylogenomic databases
eggNOGi | ENOG502QPNP, Eukaryota |
GeneTreei | ENSGT00940000155232 |
HOGENOMi | CLU_076370_0_0_1 |
InParanoidi | Q9Z0S6 |
OMAi | WILESST |
OrthoDBi | 1164514at2759 |
TreeFami | TF331936 |
Miscellaneous databases
BioGRID-ORCSi | 58187, 1 hit in 17 CRISPR screens |
ChiTaRSi | Cldn10, mouse |
PROi | PR:Q9Z0S6 |
RNActi | Q9Z0S6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022132, Expressed in submandibular gland and 200 other tissues |
Genevisiblei | Q9Z0S6, MM |
Family and domain databases
InterProi | View protein in InterPro IPR006187, Claudin IPR003554, Claudin10 IPR017974, Claudin_CS IPR004031, PMP22/EMP/MP20/Claudin |
PANTHERi | PTHR12002, PTHR12002, 1 hit PTHR12002:SF13, PTHR12002:SF13, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01383, CLAUDIN10 |
PROSITEi | View protein in PROSITE PS01346, CLAUDIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CLD10_MOUSE | |
Accessioni | Q9Z0S6Primary (citable) accession number: Q9Z0S6 Secondary accession number(s): E9PVC8 Q9CX57 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | January 22, 2014 | |
Last modified: | December 2, 2020 | |
This is version 132 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families