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Entry version 189 (26 Feb 2020)
Sequence version 2 (03 Oct 2012)
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Protein

Intersectin-1

Gene

Itsn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery (PubMed:10064583). Acts as guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex (By similarity). Plays a role in the assembly and maturation of clathrin-coated vesicles (By similarity). Recruits FCHSD2 to clathrin-coated pits (By similarity). Involved in endocytosis of activated EGFR, and probably also other growth factor receptors (PubMed:16914641). Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2 (By similarity). Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways (PubMed:16914641). In chromaffin cells, required for normal exocytosis of catecholamines (PubMed:18676989). Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones (PubMed:23633571). Inhibits ARHGAP31 activity toward RAC1 (By similarity).By similarity4 Publications
Plays a role in synaptic vesicle endocytosis in brain neurons.1 Publication

Miscellaneous

Overexpression results in the inhibition of the transferrin uptake and the blockage of the clathrin-mediated endocytosis.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) of the calcium-binding region(s) within the protein. One common calcium-binding motif is the EF-hand, but other calcium-binding motifs also exist.<p><a href='/help/ca_bind' target='_top'>More...</a></p>Calcium bindingi66 – 781PROSITE-ProRule annotationAdd BLAST13
Calcium bindingi267 – 2792PROSITE-ProRule annotationAdd BLAST13

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processEndocytosis, Exocytosis, Protein transport, Transport
LigandCalcium, Metal-binding

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-MMU-193648 NRAGE signals death through JNK
R-MMU-194840 Rho GTPase cycle
R-MMU-3928662 EPHB-mediated forward signaling
R-MMU-416482 G alpha (12/13) signalling events
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Intersectin-1
Alternative name(s):
EH and SH3 domains protein 11 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Itsn1
Synonyms:Ese11 Publication, Itsn
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 16

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:1338069 Itsn1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Coated pit, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane, Nucleus, Synapse, Synaptosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

No visible phenotype, except that about 13% of the pups do not thrive. Mice are born at the expected Mendelian rate (PubMed:18676989). Mutant mice display no obvious defects in synaptic responses to single stimuli at the calyx of Held. Repetitive stimulation gives rise to decreased synaptic responses, due to perturbation of the replenishment of release-ready synaptic vesicles (PubMed:23633571).2 Publications

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000809581 – 1714Intersectin-1Add BLAST1714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei203PhosphoserineCombined sources1
Modified residuei318PhosphoserineCombined sources1
Modified residuei334PhosphoserineCombined sources1
Modified residuei335PhosphoserineCombined sources1
Modified residuei685PhosphoserineBy similarity1
Modified residuei890PhosphothreonineCombined sources1
Modified residuei894PhosphoserineBy similarity1
Modified residuei895PhosphoserineCombined sources1
Modified residuei897PhosphoserineCombined sources1
Modified residuei971PhosphoserineBy similarity1
Modified residuei977PhosphothreonineCombined sources1
Modified residuei979PhosphoserineBy similarity1
Modified residuei988PhosphoserineBy similarity1
Modified residuei1130PhosphoserineCombined sources1
Modified residuei1137PhosphothreonineCombined sources1
Modified residuei1638PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
Q9Z0R4

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
Q9Z0R4

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q9Z0R4

PeptideAtlas

More...
PeptideAtlasi
Q9Z0R4

PRoteomics IDEntifications database

More...
PRIDEi
Q9Z0R4

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q9Z0R4

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q9Z0R4

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Detected in brain, adrenal gland and heart (PubMed:16914641, PubMed:18676989). Detected in neurons at the calyx of Held (at protein level) (PubMed:23633571). Isoform 1: Primarily detected in brain neurons. Isoform 2: Primarily detected in glia (at protein level) (PubMed:18676989). Widely expressed. Expressed at high levels in brain, heart and skeletal muscle (PubMed:10064583).4 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000022957 Expressed in floor plate of midbrain and 321 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q9Z0R4 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q9Z0R4 MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts (via DH domain) with CDC42.

Interacts (via SH3 domain 1) with WASL (By similarity).

Interacts with dynamin, SNAP25 and SNAP23 (By similarity).

Interacts with clathrin-associated proteins and other components of the endocytic machinery, such as SPIN90, EPS15, EPN1, EPN2, STON2, FCHO1, FCHO2 and DAB2 (PubMed:10064583, PubMed:20448150).

Interacts (via SH3 domains) with REPS1 and SGIP1.

Interacts with ARHGAP31 (PubMed:11744688).

Interacts with ADAM15 (By similarity).

Interacts with PRRT2 (PubMed:26797119).

Interacts (via SH3 domain 4) with FCHSD2 (via SH3 domain 2).

Interacts (via SH3 domain 1) with DENND2B (By similarity).

Interacts (via SH3 domains) with CBL (PubMed:16914641). Isoform 2:

Interacts with CBL and DNM1. Isoform 2:

Interacts with LMNA (By similarity). Isoform 2:

Interacts with importin subunit KPNA1; this is likely to mediate its import into the nucleus (PubMed:29599122).

By similarity6 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

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BioGridi
200851, 12 interactors

Protein interaction database and analysis system

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IntActi
Q9Z0R4, 17 interactors

Molecular INTeraction database

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MINTi
Q9Z0R4

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000109635

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
Q9Z0R4 protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11714
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q9Z0R4

Database of comparative protein structure models

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ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q9Z0R4

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini21 – 109EH 1PROSITE-ProRule annotationAdd BLAST89
Domaini53 – 88EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini221 – 310EH 2PROSITE-ProRule annotationAdd BLAST90
Domaini254 – 289EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini738 – 799SH3 1PROSITE-ProRule annotationAdd BLAST62
Domaini906 – 964SH3 2PROSITE-ProRule annotationAdd BLAST59
Domaini995 – 1053SH3 3PROSITE-ProRule annotationAdd BLAST59
Domaini1067 – 1131SH3 4PROSITE-ProRule annotationAdd BLAST65
Domaini1148 – 1207SH3 5PROSITE-ProRule annotationAdd BLAST60
Domaini1230 – 1416DHPROSITE-ProRule annotationAdd BLAST187
Domaini1455 – 1564PHPROSITE-ProRule annotationAdd BLAST110
Domaini1576 – 1672C2PROSITE-ProRule annotationAdd BLAST97

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni326 – 702KLERQAdd BLAST377
Regioni1067 – 1131Required for interaction with FCHSD2By similarityAdd BLAST65

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili354 – 658Sequence analysisAdd BLAST305

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi1097 – 1120Bipartite nuclear localization signal; in isoform 2By similarityAdd BLAST24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi321 – 324Poly-Ser4

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

SH3-3, SH3-4 and SH3-5, but not SH3-1 and SH3-2 domains, bind to dynamin (By similarity). SH3-1 and SH3-4 bind to ARHGAP31.By similarity
The KLERQ domain binds to SNAP-25 and SNAP-23.By similarity
In an autoinhibited form the SH3 domain 5 may bind intramolecularly to the DH domain, thus blocking the CDC42-binding site.1 Publication

Keywords - Domaini

Coiled coil, Repeat, SH3 domain

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1029 Eukaryota
KOG4305 Eukaryota
COG5422 LUCA

Ensembl GeneTree

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GeneTreei
ENSGT00940000157065

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_002819_2_0_1

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q9Z0R4

KEGG Orthology (KO)

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KOi
K20045

Database of Orthologous Groups

More...
OrthoDBi
807060at2759

TreeFam database of animal gene trees

More...
TreeFami
TF324293

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00052 EH, 2 hits
cd00160 RhoGEF, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.20.900.10, 1 hit
2.30.29.30, 1 hit
2.60.40.150, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR000261 EH_dom
IPR001331 GDS_CDC24_CS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00168 C2, 1 hit
PF12763 EF-hand_4, 2 hits
PF16652 PH_13, 1 hit
PF00621 RhoGEF, 1 hit
PF00018 SH3_1, 3 hits
PF14604 SH3_9, 2 hits

Protein Motif fingerprint database; a protein domain database

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PRINTSi
PR00452 SH3DOMAIN

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM00239 C2, 1 hit
SM00054 EFh, 2 hits
SM00027 EH, 2 hits
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SM00326 SH3, 5 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF47473 SSF47473, 2 hits
SSF48065 SSF48065, 1 hit
SSF50044 SSF50044, 5 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50004 C2, 1 hit
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS50031 EH, 2 hits
PS50003 PH_DOMAIN, 1 hit
PS50002 SH3, 5 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.

This entry has 2 described isoforms and 17 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Z0R4-1) [UniParc]FASTAAdd to basket
Also known as: Ese1L

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAQFPTPFGG SLDVWAITVE ERAKHDQQFL SLKPIAGFIT GDQARNFFFQ
60 70 80 90 100
SGLPQPVLAQ IWALADMNND GRMDQVEFSI AMKLIKLKLQ GYQLPSTLPP
110 120 130 140 150
VMKQQPVAIS SAPAFGIGGI ASMPPLTAVA PVPMGSIPVV GMSPPLVSSV
160 170 180 190 200
PPAAVPPLAN GAPPVIQPLP AFAHPAATLP KSSSFSRSGP GSQLNTKLQK
210 220 230 240 250
AQSFDVASAP PAAEWAVPQS SRLKYRQLFN SHDKTMSGHL TGPQARTILM
260 270 280 290 300
QSSLPQAQLA SIWNLSDIDQ DGKLTAEEFI LAMHLIDVAM SGQPLPPVLP
310 320 330 340 350
PEYIPPSFRR VRSGSGMSVI SSSSVDQRLP EEPSSEDEQQ PEKKLPVTFE
360 370 380 390 400
DKKRENFERG SVELEKRRQA LLEQQRKEQE RLAQLERAEQ ERKERERQEQ
410 420 430 440 450
ERKRQLELEK QLEKQRELER QREEERRKEI ERREAAKREL ERQRQLEWER
460 470 480 490 500
NRRQELLNQR NKEQEGTVVL KARRKTLEFE LEALNDKKHQ LEGKLQDIRC
510 520 530 540 550
RLATQRQEIE STNKSRELRI AEITHLQQQL QESQQMLGRL IPEKQILSDQ
560 570 580 590 600
LKQVQQNSLH RDSLLTLKRA LEAKELARQQ LREQLDEVER ETRSKLQEID
610 620 630 640 650
VFNNQLKELR EIHSKQQLQK QRSLEAARLK QKEQERKSLE LEKQKEDAQR
660 670 680 690 700
RVQERDKQWL EHVQQEEQPR PRKPHEEDRL KREDSVRKKE AEERAKPEMQ
710 720 730 740 750
DKQSRLFHPH QEPAKLATQA PWSTTEKGPL TISAQESVKV VYYRALYPFE
760 770 780 790 800
SRSHDEITIQ PGDIVMVDES QTGEPGWLGG ELKGKTGWFP ANYAEKIPEN
810 820 830 840 850
EVPTPAKPVT DLTSAPAPKL ALRETPAPLP VTSSEPSTTP NNWADFSSTW
860 870 880 890 900
PSSSNEKPET DNWDTWAAQP SLTVPSAGQL RQRSAFTPAT ATGSSPSPVL
910 920 930 940 950
GQGEKVEGLQ AQALYPWRAK KDNHLNFNKS DVITVLEQQD MWWFGEVQGQ
960 970 980 990 1000
KGWFPKSYVK LISGPVRKST SIDTGPTESP ASLKRVASPA AKPAIPGEEF
1010 1020 1030 1040 1050
IAMYTYESSE QGDLTFQQGD VIVVTKKDGD WWTGTVGDKS GVFPSNYVRL
1060 1070 1080 1090 1100
KDSEGSGTAG KTGSLGKKPE IAQVIASYAA TGPEQLTLAP GQLILIRKKN
1110 1120 1130 1140 1150
PGGWWEGELQ ARGKKRQIGW FPANYVKLLS PGTSKITPTE LPKTAVQPAV
1160 1170 1180 1190 1200
CQVIGMYDYT AQNDDELAFS KGQIINVLNK EDPDWWKGEV SGQVGLFPSN
1210 1220 1230 1240 1250
YVKLTTDMDP SQQWCSDLHL LDMLTPTERK RQGYIHELIV TEENYVNDLQ
1260 1270 1280 1290 1300
LVTEIFQKPL TESELLTEKE VAMIFVNWKE LIMCNIKLLK ALRVRKKMSG
1310 1320 1330 1340 1350
EKMPVKMIGD ILSAQLPHMQ PYIRFCSCQL NGAALIQQKT DEAPDFKEFV
1360 1370 1380 1390 1400
KRLAMDPRCK GMPLSSFILK PMQRVTRYPL IIKNILENTP ENHPDHSHLK
1410 1420 1430 1440 1450
HALEKAEELC SQVNEGVREK ENSDRLEWIQ AHVQCEGLSE QLVFNSVTNC
1460 1470 1480 1490 1500
LGPRKFLHSG KLYKAKSNKE LYGFLFNDFL LLTQITKPLG SSGTDKVFSP
1510 1520 1530 1540 1550
KSNLQYKMYK TPIFLNEVLV KLPTDPSGDE PIFHISHIDR VYTLRAESIN
1560 1570 1580 1590 1600
ERTAWVQKIK AASELYIETE KKKREKAYLV RSQRATGIGR LMVNVVEGIE
1610 1620 1630 1640 1650
LKPCRSHGKS NPYCEVTMGS QCHITKTIQD TLNPKWNSNC QFFIRDLEQE
1660 1670 1680 1690 1700
VLCITVFERD QFSPDDFLGR TEIRVADIKK DQGSKGPVTK CLLLHEVPTG
1710
EIVVRLDLQL FDEP
Length:1,714
Mass (Da):194,297
Last modified:October 3, 2012 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iBCF5038160E8208E
GO
Isoform 2 (identifier: Q9Z0R4-2) [UniParc]FASTAAdd to basket
Also known as: ITSN-sBy similarity

The sequence of this isoform differs from the canonical sequence as follows:
     1214-1714: Missing.

Show »
Length:1,213
Mass (Da):136,607
Checksum:i1376AD0DFBD503C2
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 17 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q0N0E9Q0N0_MOUSE
Intersectin-1
Itsn1
1,719Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9Q3I4E9Q3I4_MOUSE
Intersectin-1
Itsn1
1,176Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
Q6NZJ5Q6NZJ5_MOUSE
Intersectin-1
Itsn1
621Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9Q3I5E9Q3I5_MOUSE
Intersectin-1
Itsn1
1,218Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9Q3I8E9Q3I8_MOUSE
Intersectin-1
Itsn1
1,142Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9Q3I9E9Q3I9_MOUSE
Intersectin-1
Itsn1
1,147Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
D3Z6P4D3Z6P4_MOUSE
Intersectin-1
Itsn1
359Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F6R6C8F6R6C8_MOUSE
Intersectin-1
Itsn1
256Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A338P6K7A0A338P6K7_MOUSE
Intersectin-1
Itsn1
187Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A338P6L8A0A338P6L8_MOUSE
Intersectin-1
Itsn1
131Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
There are more potential isoformsShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti179L → W in AAD19749 (PubMed:10064583).Curated1
Sequence conflicti179L → W in AAD19746 (PubMed:10064583).Curated1
Sequence conflicti402R → A in AAD19749 (PubMed:10064583).Curated1
Sequence conflicti402R → A in AAD19746 (PubMed:10064583).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0042961214 – 1714Missing in isoform 2. 1 PublicationAdd BLAST501

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF132481 mRNA Translation: AAD19749.1
AF132478 mRNA Translation: AAD19746.1
AC126053 Genomic DNA No translation available.
AC131691 Genomic DNA No translation available.
AC134837 Genomic DNA No translation available.
AF169621 mRNA Translation: AAD48848.1
AF356517 Genomic DNA Translation: AAK40228.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS37402.1 [Q9Z0R4-1]
CCDS49913.1 [Q9Z0R4-2]

NCBI Reference Sequences

More...
RefSeqi
NP_001103745.1, NM_001110275.1 [Q9Z0R4-2]
NP_034717.2, NM_010587.2 [Q9Z0R4-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSMUST00000056482; ENSMUSP00000056011; ENSMUSG00000022957 [Q9Z0R4-2]
ENSMUST00000114002; ENSMUSP00000109635; ENSMUSG00000022957 [Q9Z0R4-1]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
16443

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mmu:16443

UCSC genome browser

More...
UCSCi
uc007zyi.2 mouse [Q9Z0R4-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132481 mRNA Translation: AAD19749.1
AF132478 mRNA Translation: AAD19746.1
AC126053 Genomic DNA No translation available.
AC131691 Genomic DNA No translation available.
AC134837 Genomic DNA No translation available.
AF169621 mRNA Translation: AAD48848.1
AF356517 Genomic DNA Translation: AAK40228.1
CCDSiCCDS37402.1 [Q9Z0R4-1]
CCDS49913.1 [Q9Z0R4-2]
RefSeqiNP_001103745.1, NM_001110275.1 [Q9Z0R4-2]
NP_034717.2, NM_010587.2 [Q9Z0R4-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3HS8X-ray1.90P840-851[»]
3JV3X-ray2.40A/B1151-1431[»]
SMRiQ9Z0R4
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGridi200851, 12 interactors
IntActiQ9Z0R4, 17 interactors
MINTiQ9Z0R4
STRINGi10090.ENSMUSP00000109635

PTM databases

iPTMnetiQ9Z0R4
PhosphoSitePlusiQ9Z0R4

Proteomic databases

EPDiQ9Z0R4
jPOSTiQ9Z0R4
PaxDbiQ9Z0R4
PeptideAtlasiQ9Z0R4
PRIDEiQ9Z0R4

Genome annotation databases

EnsembliENSMUST00000056482; ENSMUSP00000056011; ENSMUSG00000022957 [Q9Z0R4-2]
ENSMUST00000114002; ENSMUSP00000109635; ENSMUSG00000022957 [Q9Z0R4-1]
GeneIDi16443
KEGGimmu:16443
UCSCiuc007zyi.2 mouse [Q9Z0R4-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
6453
MGIiMGI:1338069 Itsn1

Phylogenomic databases

eggNOGiKOG1029 Eukaryota
KOG4305 Eukaryota
COG5422 LUCA
GeneTreeiENSGT00940000157065
HOGENOMiCLU_002819_2_0_1
InParanoidiQ9Z0R4
KOiK20045
OrthoDBi807060at2759
TreeFamiTF324293

Enzyme and pathway databases

ReactomeiR-MMU-193648 NRAGE signals death through JNK
R-MMU-194840 Rho GTPase cycle
R-MMU-3928662 EPHB-mediated forward signaling
R-MMU-416482 G alpha (12/13) signalling events
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
Itsn1 mouse
EvolutionaryTraceiQ9Z0R4

Protein Ontology

More...
PROi
PR:Q9Z0R4
RNActiQ9Z0R4 protein

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000022957 Expressed in floor plate of midbrain and 321 other tissues
ExpressionAtlasiQ9Z0R4 baseline and differential
GenevisibleiQ9Z0R4 MM

Family and domain databases

CDDicd00052 EH, 2 hits
cd00160 RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR000261 EH_dom
IPR001331 GDS_CDC24_CS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF12763 EF-hand_4, 2 hits
PF16652 PH_13, 1 hit
PF00621 RhoGEF, 1 hit
PF00018 SH3_1, 3 hits
PF14604 SH3_9, 2 hits
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00054 EFh, 2 hits
SM00027 EH, 2 hits
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SM00326 SH3, 5 hits
SUPFAMiSSF47473 SSF47473, 2 hits
SSF48065 SSF48065, 1 hit
SSF50044 SSF50044, 5 hits
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS50031 EH, 2 hits
PS50003 PH_DOMAIN, 1 hit
PS50002 SH3, 5 hits

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiITSN1_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9Z0R4
Secondary accession number(s): F8VQE5, Q9R143
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 3, 2012
Last modified: February 26, 2020
This is version 189 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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