UniProtKB - Q9Z0P7 (SUFU_MOUSE)
Suppressor of fused homolog
Sufu
Functioni
GO - Molecular functioni
- beta-catenin binding Source: MGI
- protein kinase binding Source: MGI
- transcription factor binding Source: MGI
GO - Biological processi
- aorta development Source: MGI
- coronary vasculature development Source: MGI
- cytoplasmic sequestering of transcription factor Source: MGI
- determination of left/right symmetry Source: MGI
- heart looping Source: MGI
- negative regulation of DNA-binding transcription factor activity Source: GO_Central
- negative regulation of smoothened signaling pathway Source: MGI
- negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- negative regulation of ubiquitin-dependent protein catabolic process Source: MGI
- neural tube closure Source: MGI
- skin development Source: MGI
- smoothened signaling pathway Source: MGI
- smoothened signaling pathway involved in spinal cord motor neuron cell fate specification Source: MGI
- smoothened signaling pathway involved in ventral spinal cord interneuron specification Source: MGI
- spinal cord dorsal/ventral patterning Source: MGI
- ventricular septum development Source: MGI
Keywordsi
Molecular function | Developmental protein |
Enzyme and pathway databases
Reactomei | R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5610787, Hedgehog 'off' state R-MMU-5632684, Hedgehog 'on' state |
Names & Taxonomyi
Protein namesi | Recommended name: Suppressor of fused homolog1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1345643, Sufu |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000072303 | 1 – 484 | Suppressor of fused homologAdd BLAST | 484 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 257 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 301 | PhosphoserineBy similarity | 1 | |
Modified residuei | 303 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 321 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 342 | PhosphoserineBy similarity | 1 | |
Modified residuei | 346 | PhosphoserineCombined sources | 1 | |
Modified residuei | 352 | PhosphoserineBy similarity | 1 | |
Modified residuei | 353 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 481 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q9Z0P7 |
PaxDbi | Q9Z0P7 |
PeptideAtlasi | Q9Z0P7 |
PRIDEi | Q9Z0P7 |
PTM databases
iPTMneti | Q9Z0P7 |
PhosphoSitePlusi | Q9Z0P7 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000025231, Expressed in ear vesicle and 295 other tissues |
ExpressionAtlasi | Q9Z0P7, baseline and differential |
Genevisiblei | Q9Z0P7, MM |
Interactioni
Subunit structurei
May form homodimers (By similarity).
Interacts with ULK3; inactivating the protein kinase activity of ULK3.
Interacts with RAB23 (By similarity).
Part of a DNA-bound corepressor complex containing SAP18, GLI1 and SIN3 (PubMed:11960000).
Part of a complex containing CTNNB1 (PubMed:11477086). Binds BTRC, GLI2, GLI3, SAP18 and STK36 (PubMed:20360384, PubMed:23034632). Binds both free and DNA-bound GLI1 (PubMed:10531011).
Interacts with KIF7 (PubMed:19592253).
Interacts with GLI3FL and this interaction regulates the formation of either repressor or activator forms of GLI3 (PubMed:20360384). Its association with GLI3FL is regulated by Hh signaling and dissociation of the SUFU-GLI3 interaction requires the presence of the ciliary motor KIF3A (PubMed:20360384).
By similarity6 PublicationsBinary interactionsi
Hide detailsQ9Z0P7
With | #Exp. | IntAct |
---|---|---|
Lgals3 [P16110] | 5 | EBI-3508336,EBI-3508325 |
Pias1 [O88907] | 3 | EBI-3508336,EBI-3508327 |
Rnf19a [P50636] | 3 | EBI-3508336,EBI-3508340 |
Sap18 [O55128] | 6 | EBI-3508336,EBI-3508332 |
GO - Molecular functioni
- beta-catenin binding Source: MGI
- protein kinase binding Source: MGI
- transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204881, 24 interactors |
ComplexPortali | CPX-147, GLI1-SUFU complex CPX-149, GLI2-SUFU complex CPX-151, GLI3-SUFU complex |
CORUMi | Q9Z0P7 |
IntActi | Q9Z0P7, 21 interactors |
STRINGi | 10090.ENSMUSP00000107498 |
Miscellaneous databases
RNActi | Q9Z0P7, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 279 – 360 | Intrinsically disorderedBy similarityAdd BLAST | 82 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QT57, Eukaryota |
GeneTreei | ENSGT00390000009747 |
HOGENOMi | CLU_033906_0_0_1 |
InParanoidi | Q9Z0P7 |
OMAi | GDNIPWR |
PhylomeDBi | Q9Z0P7 |
TreeFami | TF324548 |
Family and domain databases
DisProti | DP01397 |
Gene3Di | 3.30.1360.230, 1 hit |
InterProi | View protein in InterPro IPR020941, SUFU-like_domain IPR024314, SUFU_C IPR038489, SUFU_C_sf IPR037181, SUFU_N IPR007768, Suppressor_of_fused IPR016591, Suppressor_of_fused_euk |
PANTHERi | PTHR10928, PTHR10928, 1 hit |
Pfami | View protein in Pfam PF05076, SUFU, 1 hit PF12470, SUFU_C, 1 hit |
PIRSFi | PIRSF011844, Suppressor_of_fused_protein, 1 hit |
SUPFAMi | SSF103359, SSF103359, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAELRPSVAP GPAAPPASGP SAPPAFASLF PPGLHAIYGE CRRLYPDQPN
60 70 80 90 100
PLQVTAIVKY WLGGPDPLDY VSMYRNMGSP SANIPEHWHY ISFGLSDLYG
110 120 130 140 150
DNRVHEFTGT DGPSGFGFEL TFRLKRETGE SAPPTWPAEL MQGLARYVFQ
160 170 180 190 200
SENTFCSGDH VSWHSPLDNS ESRIQHMLLT EDPQMQPVRT PFGVVTFLQI
210 220 230 240 250
VGVCTEELHS AQQWNGQGIL ELLRTVPIAG GPWLITDMRR GETIFEIDPH
260 270 280 290 300
LQERVDKGIE TDGSNLSGVS AKCAWDDLSR PPEDEEDSRS ICLGTQPRRL
310 320 330 340 350
SGKDTEQIRE TLRRGLEINS KPVLPPINSQ RQNGLTHDRA PSRKDSLGSD
360 370 380 390 400
SSTAIIPHEL IRTRQLESVH LKFNQESGAL IPLCLRGRLL HGRHFTYKSI
410 420 430 440 450
TGDMAITFVS TGVEGAFATE EHPYAAHGPW LQILLTEEFV EKMLEDLEDL
460 470 480
TSPEEFKLPK EYSWPEKKLK VSILPDVVFD SPLH
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3Z7B6 | D3Z7B6_MOUSE | Suppressor of fused homolog | Sufu | 467 | Annotation score: | ||
A0A0R4J1M0 | A0A0R4J1M0_MOUSE | Suppressor of fused homolog | Sufu | 297 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 79 | S → C in AAF61412 (PubMed:10531011).Curated | 1 | |
Sequence conflicti | 220 | L → Q in AAF61412 (PubMed:10531011).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013281 | 62 – 106 | Missing in isoform 2. 1 PublicationAdd BLAST | 45 | |
Alternative sequenceiVSP_013282 | 252 | Q → QQ in isoform 2, isoform 3 and isoform 4. 2 Publications | 1 | |
Alternative sequenceiVSP_013283 | 433 – 484 | ILLTE…DSPLH → VRRSLSSFSSSSCSSLAACP PLPHHPKDRPLWLPC in isoform 3. 1 PublicationAdd BLAST | 52 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF134893 mRNA Translation: AAF61412.1 AJ131692 mRNA Translation: CAB38081.1 AJ308625 mRNA Translation: CAC34257.1 AJ308626 mRNA Translation: CAC34258.1 AJ308627 , AJ308628, AJ308629, AJ308630, AJ308632, AJ308634, AJ308635, AJ308633, AJ308631 Genomic DNA Translation: CAC34271.1 AK015885 mRNA Translation: BAB30017.1 AK047603 mRNA Translation: BAC33095.1 BC048168 mRNA Translation: AAH48168.1 BC056997 mRNA Translation: AAH56997.1 |
CCDSi | CCDS29879.1 [Q9Z0P7-1] CCDS38008.1 [Q9Z0P7-4] |
RefSeqi | NP_001020562.1, NM_001025391.2 [Q9Z0P7-1] NP_056567.2, NM_015752.3 [Q9Z0P7-4] |
Genome annotation databases
Ensembli | ENSMUST00000039922; ENSMUSP00000049109; ENSMUSG00000025231 [Q9Z0P7-1] ENSMUST00000111867; ENSMUSP00000107498; ENSMUSG00000025231 [Q9Z0P7-4] |
GeneIDi | 24069 |
KEGGi | mmu:24069 |
UCSCi | uc008htn.2, mouse [Q9Z0P7-2] uc008hto.2, mouse [Q9Z0P7-3] uc008htq.2, mouse [Q9Z0P7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF134893 mRNA Translation: AAF61412.1 AJ131692 mRNA Translation: CAB38081.1 AJ308625 mRNA Translation: CAC34257.1 AJ308626 mRNA Translation: CAC34258.1 AJ308627 , AJ308628, AJ308629, AJ308630, AJ308632, AJ308634, AJ308635, AJ308633, AJ308631 Genomic DNA Translation: CAC34271.1 AK015885 mRNA Translation: BAB30017.1 AK047603 mRNA Translation: BAC33095.1 BC048168 mRNA Translation: AAH48168.1 BC056997 mRNA Translation: AAH56997.1 |
CCDSi | CCDS29879.1 [Q9Z0P7-1] CCDS38008.1 [Q9Z0P7-4] |
RefSeqi | NP_001020562.1, NM_001025391.2 [Q9Z0P7-1] NP_056567.2, NM_015752.3 [Q9Z0P7-4] |
3D structure databases
SMRi | Q9Z0P7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204881, 24 interactors |
ComplexPortali | CPX-147, GLI1-SUFU complex CPX-149, GLI2-SUFU complex CPX-151, GLI3-SUFU complex |
CORUMi | Q9Z0P7 |
IntActi | Q9Z0P7, 21 interactors |
STRINGi | 10090.ENSMUSP00000107498 |
PTM databases
iPTMneti | Q9Z0P7 |
PhosphoSitePlusi | Q9Z0P7 |
Proteomic databases
jPOSTi | Q9Z0P7 |
PaxDbi | Q9Z0P7 |
PeptideAtlasi | Q9Z0P7 |
PRIDEi | Q9Z0P7 |
Protocols and materials databases
Antibodypediai | 1768, 276 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000039922; ENSMUSP00000049109; ENSMUSG00000025231 [Q9Z0P7-1] ENSMUST00000111867; ENSMUSP00000107498; ENSMUSG00000025231 [Q9Z0P7-4] |
GeneIDi | 24069 |
KEGGi | mmu:24069 |
UCSCi | uc008htn.2, mouse [Q9Z0P7-2] uc008hto.2, mouse [Q9Z0P7-3] uc008htq.2, mouse [Q9Z0P7-1] |
Organism-specific databases
CTDi | 51684 |
MGIi | MGI:1345643, Sufu |
Phylogenomic databases
eggNOGi | ENOG502QT57, Eukaryota |
GeneTreei | ENSGT00390000009747 |
HOGENOMi | CLU_033906_0_0_1 |
InParanoidi | Q9Z0P7 |
OMAi | GDNIPWR |
PhylomeDBi | Q9Z0P7 |
TreeFami | TF324548 |
Enzyme and pathway databases
Reactomei | R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5610787, Hedgehog 'off' state R-MMU-5632684, Hedgehog 'on' state |
Miscellaneous databases
BioGRID-ORCSi | 24069, 6 hits in 20 CRISPR screens |
ChiTaRSi | Sufu, mouse |
PROi | PR:Q9Z0P7 |
RNActi | Q9Z0P7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025231, Expressed in ear vesicle and 295 other tissues |
ExpressionAtlasi | Q9Z0P7, baseline and differential |
Genevisiblei | Q9Z0P7, MM |
Family and domain databases
DisProti | DP01397 |
Gene3Di | 3.30.1360.230, 1 hit |
InterProi | View protein in InterPro IPR020941, SUFU-like_domain IPR024314, SUFU_C IPR038489, SUFU_C_sf IPR037181, SUFU_N IPR007768, Suppressor_of_fused IPR016591, Suppressor_of_fused_euk |
PANTHERi | PTHR10928, PTHR10928, 1 hit |
Pfami | View protein in Pfam PF05076, SUFU, 1 hit PF12470, SUFU_C, 1 hit |
PIRSFi | PIRSF011844, Suppressor_of_fused_protein, 1 hit |
SUPFAMi | SSF103359, SSF103359, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SUFU_MOUSE | |
Accessioni | Q9Z0P7Primary (citable) accession number: Q9Z0P7 Secondary accession number(s): Q8C8B4 Q9JLU1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2005 |
Last sequence update: | May 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families