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UniProtKB - Q9Z0M1 (KLK4_MOUSE)
Protein
Kallikrein-4
Gene
Klk4
Organism
Mus musculus (Mouse)
Status
Functioni
Has a major role in enamel formation. Required during the maturation stage of tooth development for clearance of enamel proteins and normal structural patterning of the crystalline matrix.
1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 41 | ZincBy similarity | 1 | |
Active sitei | 72 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Metal bindingi | 92 | ZincBy similarity | 1 | |
Active sitei | 117 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 208 | Charge relay systemPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- serine-type endopeptidase activity Source: InterPro
GO - Biological processi
- amelogenesis Source: MGI
- biomineral tissue development Source: UniProtKB-KW
- extracellular matrix disassembly Source: MGI
- protein catabolic process Source: MGI
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Biomineralization |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.4.21.B12, 3474 |
Protein family/group databases
MEROPSi | S01.251 |
Names & Taxonomyi
Protein namesi | Recommended name: Kallikrein-4By similarity (EC:3.4.21.-PROSITE-ProRule annotation)Alternative name(s): Enamel matrix serine proteinase 11 Publication Kallikrein-like protein 1By similarity Serine protease 171 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1861379, Klk4 |
VEuPathDBi | HostDB:ENSMUSG00000006948 |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Other locations
- secretory granule Source: GO_Central
Keywords - Cellular componenti
SecretedPathology & Biotechi
Disruption phenotypei
Viable and fertile, when reared on a soft diet. Tooth morphology is grossly normal but the enamel surface is fragile and rapidly abraded. Although formation of the enamel layer is initially normal, the crystallites fail to thicken and interlock. The enamel proteins enamelin and amelogenin are not cleared and persist in the matrix during the maturation stage.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
PropeptideiPRO_0000436024 | 26 – 31 | By similarity | 6 | |
ChainiPRO_5006493980 | 32 – 255 | Kallikrein-4Sequence analysisAdd BLAST | 224 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 57 ↔ 73 | PROSITE-ProRule annotation | ||
Glycosylationi | 124 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 149 ↔ 214 | PROSITE-ProRule annotation | ||
Glycosylationi | 170 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 179 ↔ 193 | PROSITE-ProRule annotation | ||
Disulfide bondi | 204 ↔ 229 | PROSITE-ProRule annotation |
Post-translational modificationi
N-glycosylated. The N-glycan structures are of complex diantennary or triantennary type, which may be further modified with up to 2 sialic acid residues.1 Publication
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | Q9Z0M1 |
ProteomicsDBi | 264852 |
PTM databases
GlyGeni | Q9Z0M1, 2 sites |
PhosphoSitePlusi | Q9Z0M1 |
Expressioni
Developmental stagei
In developing teeth, expressed in ameloblasts during transition and maturation stages (PubMed:10863090, PubMed:10690663, PubMed:19578120). Expressed weakly in odontoblasts (PubMed:10863090). Not detected in odontoblasts (PubMed:19578120). Detected in the epithelium surrounding the erupted first molar (PubMed:10690663).3 Publications
Gene expression databases
Bgeei | ENSMUSG00000006948, Expressed in molar tooth and 41 other tissues |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000007161 |
Miscellaneous databases
RNActi | Q9Z0M1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 253 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 222 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT01020000230389 |
HOGENOMi | CLU_006842_1_1_1 |
InParanoidi | Q9Z0M1 |
OMAi | QCPTAGD |
OrthoDBi | 1314811at2759 |
PhylomeDBi | Q9Z0M1 |
TreeFami | TF331065 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9Z0M1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMVTARTPWG WFLGCLILEV TGASASSVSS RIIQGQDCSP HSQPWQAALF
60 70 80 90 100
SEDGFFCSGV LVHPQWVLSA AHCLQESYIV GLGLHNLKGS QEPGSRMLEA
110 120 130 140 150
HLSIQHPNFN DPSFANDLML IKLNESVIES NTIRSIPVAT QCPTPGDTCL
160 170 180 190 200
VSGWGQLKNG KLPSLLQCVN LSVASEETCR LLYDPVYHLS MFCAGGGQDQ
210 220 230 240 250
KDSCNGDSGG PIVCNRSLQG LVSMGQGKCG QPGIPSVYTN LCKFTNWIQT
IIQTN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | C → Y in AAF85937 (PubMed:10863090).Curated | 1 | |
Sequence conflicti | 251 | I → T in AAF85937 (PubMed:10863090).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF198031 Genomic DNA Translation: AAF85937.1 AF019979 mRNA Translation: AAC98894.1 AC134858 Genomic DNA No translation available. CH466603 Genomic DNA Translation: EDL22687.1 BC100716 mRNA Translation: AAI00717.1 BC100717 mRNA Translation: AAI00718.1 BC100718 mRNA Translation: AAI00719.1 BC100719 mRNA Translation: AAI00720.1 |
CCDSi | CCDS21186.1 |
RefSeqi | NP_064312.1, NM_019928.1 |
Genome annotation databases
Ensembli | ENSMUST00000007161; ENSMUSP00000007161; ENSMUSG00000006948 |
GeneIDi | 56640 |
KEGGi | mmu:56640 |
UCSCi | uc009gny.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF198031 Genomic DNA Translation: AAF85937.1 AF019979 mRNA Translation: AAC98894.1 AC134858 Genomic DNA No translation available. CH466603 Genomic DNA Translation: EDL22687.1 BC100716 mRNA Translation: AAI00717.1 BC100717 mRNA Translation: AAI00718.1 BC100718 mRNA Translation: AAI00719.1 BC100719 mRNA Translation: AAI00720.1 |
CCDSi | CCDS21186.1 |
RefSeqi | NP_064312.1, NM_019928.1 |
3D structure databases
SMRi | Q9Z0M1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000007161 |
Protein family/group databases
MEROPSi | S01.251 |
PTM databases
GlyGeni | Q9Z0M1, 2 sites |
PhosphoSitePlusi | Q9Z0M1 |
Proteomic databases
PaxDbi | Q9Z0M1 |
ProteomicsDBi | 264852 |
Protocols and materials databases
Antibodypediai | 18932, 296 antibodies from 34 providers |
DNASUi | 56640 |
Genome annotation databases
Ensembli | ENSMUST00000007161; ENSMUSP00000007161; ENSMUSG00000006948 |
GeneIDi | 56640 |
KEGGi | mmu:56640 |
UCSCi | uc009gny.1, mouse |
Organism-specific databases
CTDi | 9622 |
MGIi | MGI:1861379, Klk4 |
VEuPathDBi | HostDB:ENSMUSG00000006948 |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT01020000230389 |
HOGENOMi | CLU_006842_1_1_1 |
InParanoidi | Q9Z0M1 |
OMAi | QCPTAGD |
OrthoDBi | 1314811at2759 |
PhylomeDBi | Q9Z0M1 |
TreeFami | TF331065 |
Enzyme and pathway databases
BRENDAi | 3.4.21.B12, 3474 |
Miscellaneous databases
BioGRID-ORCSi | 56640, 2 hits in 64 CRISPR screens |
PROi | PR:Q9Z0M1 |
RNActi | Q9Z0M1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000006948, Expressed in molar tooth and 41 other tissues |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KLK4_MOUSE | |
Accessioni | Q9Z0M1Primary (citable) accession number: Q9Z0M1 Secondary accession number(s): Q9JIS2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2016 |
Last sequence update: | May 1, 1999 | |
Last modified: | February 23, 2022 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families