Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Gamma-glutamyl hydrolase

Gene

Ggh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Acts as endopeptidase. Lysosomal enzyme is activated by sulfhydryl compounds.

Catalytic activityi

Hydrolysis of a gamma-glutamyl bond.

pH dependencei

Optimum pH is 4. Active from pH 3 to 5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei133NucleophilePROSITE-ProRule annotation1
Active sitei243Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Protein family/group databases

MEROPSiC26.001

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyl hydrolase (EC:3.4.19.9)
Alternative name(s):
Conjugase
FGPH
Folylpolyglutamate hydrolase
GH
Gamma-Glu-x carboxypeptidase
Gene namesi
Name:Ggh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1329035 Ggh

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24By similarityAdd BLAST24
ChainiPRO_000002654025 – 317Gamma-glutamyl hydrolaseAdd BLAST293

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi100N-linked (GlcNAc...) (high mannose) asparagine1 Publication1
Glycosylationi162N-linked (GlcNAc...) (high mannose) asparagine1 Publication1
Glycosylationi188N-linked (GlcNAc...) (high mannose) asparagine1 Publication1
Glycosylationi202N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi306N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9Z0L8
PRIDEiQ9Z0L8

PTM databases

iPTMnetiQ9Z0L8
PhosphoSitePlusiQ9Z0L8
UniCarbKBiQ9Z0L8

Expressioni

Tissue specificityi

Isoform I (more expressed than isoform II in all tissues) is highly expressed in salivary gland, followed by kidney, liver, lung, stomach and uterus, and weakly expressed in small intestine, brain and fetal liver. Also expressed at a lower level in thymus, spleen and skeletal muscle. Also expressed in tumors.

Gene expression databases

BgeeiENSMUSG00000073987
CleanExiMM_GGH
GenevisibleiQ9Z0L8 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000095843

Structurei

3D structure databases

ProteinModelPortaliQ9Z0L8
SMRiQ9Z0L8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 317Gamma-glutamyl hydrolasePROSITE-ProRule annotationAdd BLAST293

Sequence similaritiesi

Belongs to the peptidase C26 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1559 Eukaryota
ENOG410XQKI LUCA
GeneTreeiENSGT00490000043388
HOGENOMiHOG000006721
HOVERGENiHBG005833
InParanoidiQ9Z0L8
KOiK01307
OMAiGILMQKC
OrthoDBiEOG091G0E82
TreeFamiTF323437

Family and domain databases

Gene3Di3.40.50.880, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR015527 Pept_C26_g-glut_hydrolase
IPR011697 Peptidase_C26
PANTHERiPTHR11315 PTHR11315, 1 hit
PfamiView protein in Pfam
PF07722 Peptidase_C26, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
PROSITEiView protein in PROSITE
PS51275 PEPTIDASE_C26_GGH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform I (identifier: Q9Z0L8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANLGYLLCL LGLLLCGLSS PGMSRPYNHG SERPIIGVVM QECFGKMAKL
60 70 80 90 100
GNYYIAASYV KYIESAGARV VPIRPDLSDA EYEELFRSIN GVLLPGGGAN
110 120 130 140 150
LTDSGYSRVA KIFFSKALES FDNGDHFPVW GTCLGFEELS VLVSGENLLT
160 170 180 190 200
STDTKSKKLP LNFTEGARKS RMFKHFPTEL LDSLALENLT ANFHKWSLSV
210 220 230 240 250
KNFTENEKLK KFFNILTTNT DGKTEFISSM EGFKYPVYAV QWHPEKAAFE
260 270 280 290 300
WKNLGGISHA PNAVKTSFYL AEFLVSEARK NSHHFENVVK ETASLIYKFN
310
PIYTGNISSF QQAYMFD
Length:317
Mass (Da):35,470
Last modified:July 27, 2011 - v2
Checksum:i139F56E1EFE6F801
GO
Isoform II (identifier: Q9Z0L8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: MANLGYLLCLLGLLLC → MVRGWRLLGVLMLS

Show »
Length:315
Mass (Da):35,377
Checksum:i331F7F9C9D2A9B62
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti125D → G in AAC70002 (PubMed:9756990).Curated1
Sequence conflicti125D → G in AAC70003 (PubMed:9756990).Curated1
Sequence conflicti125D → G in AAD47388 (PubMed:10393243).Curated1
Sequence conflicti170 – 171SR → NK in AAC70002 (PubMed:9756990).Curated2
Sequence conflicti170 – 171SR → NK in AAC70003 (PubMed:9756990).Curated2
Sequence conflicti170 – 171SR → NK in AAD47388 (PubMed:10393243).Curated2
Sequence conflicti248A → P in AAC70002 (PubMed:9756990).Curated1
Sequence conflicti248A → P in AAC70003 (PubMed:9756990).Curated1
Sequence conflicti248A → P in AAD47388 (PubMed:10393243).Curated1
Sequence conflicti299 – 300FN → VY in AAC70002 (PubMed:9756990).Curated2
Sequence conflicti299 – 300FN → VY in AAC70003 (PubMed:9756990).Curated2
Sequence conflicti299 – 300FN → VY in AAD47388 (PubMed:10393243).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0054471 – 16MANLG…GLLLC → MVRGWRLLGVLMLS in isoform II. CuratedAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051102 mRNA Translation: AAC70002.1
AF051103 mRNA Translation: AAC70003.1
AF090732
, AF090725, AF090726, AF090727, AF090728, AF090729, AF090730, AF090731 Genomic DNA Translation: AAD47388.1
AL732527, CR931798 Genomic DNA Translation: CAM18838.2
CR931798, AL732527 Genomic DNA Translation: CAO78172.1
CCDSiCCDS51126.1 [Q9Z0L8-1]
RefSeqiNP_034411.2, NM_010281.2 [Q9Z0L8-1]
UniGeneiMm.20461

Genome annotation databases

EnsembliENSMUST00000098242; ENSMUSP00000095843; ENSMUSG00000073987 [Q9Z0L8-1]
GeneIDi14590
KEGGimmu:14590
UCSCiuc008sck.1 mouse [Q9Z0L8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGGH_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0L8
Secondary accession number(s): B1AWC1, Q9Z0L7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health