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Protein

Cell cycle checkpoint control protein RAD9A

Gene

Rad9a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity (By similarity).By similarity

Catalytic activityi

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: MGI
  • cellular response to ionizing radiation Source: MGI
  • DNA damage checkpoint Source: MGI
  • DNA repair Source: InterPro
  • intra-S DNA damage checkpoint Source: MGI
  • positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease
Biological processDNA damage

Enzyme and pathway databases

ReactomeiR-MMU-176187 Activation of ATR in response to replication stress
R-MMU-5685938 HDR through Single Strand Annealing (SSA)
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-69473 G2/M DNA damage checkpoint

Names & Taxonomyi

Protein namesi
Recommended name:
Cell cycle checkpoint control protein RAD9A (EC:3.1.11.2)
Short name:
mRAD9
Alternative name(s):
DNA repair exonuclease rad9 homolog A
Rad9-like protein
Gene namesi
Name:Rad9a
Synonyms:Rad9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1328356 Rad9a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002250011 – 389Cell cycle checkpoint control protein RAD9AAdd BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphotyrosineBy similarity1
Modified residuei272PhosphoserineBy similarity1
Modified residuei277PhosphoserineBy similarity1
Modified residuei341PhosphoserineBy similarity1
Modified residuei373PhosphoserineCombined sources1
Modified residuei385PhosphoserineCombined sources1

Post-translational modificationi

Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Z0F6
MaxQBiQ9Z0F6
PaxDbiQ9Z0F6
PeptideAtlasiQ9Z0F6
PRIDEiQ9Z0F6

PTM databases

iPTMnetiQ9Z0F6
PhosphoSitePlusiQ9Z0F6

Expressioni

Tissue specificityi

Expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000024824 Expressed in 217 organ(s), highest expression level in bone marrow
CleanExiMM_RAD9
GenevisibleiQ9Z0F6 MM

Interactioni

Subunit structurei

Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1 and RPA2. Interacts with DNAJC7 and RHNO1 (By similarity). Interacts with ATAD5.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202570, 2 interactors
STRINGi10090.ENSMUSP00000025740

Structurei

3D structure databases

ProteinModelPortaliQ9Z0F6
SMRiQ9Z0F6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 91Possesses 3'-5' exonuclease activityBy similarityAdd BLAST41
Regioni266 – 389Sufficient for interaction with ABL1By similarityAdd BLAST124

Sequence similaritiesi

Belongs to the rad9 family.Curated

Phylogenomic databases

eggNOGiKOG2810 Eukaryota
ENOG410XYYN LUCA
GeneTreeiENSGT00390000005767
HOGENOMiHOG000059650
HOVERGENiHBG058989
InParanoidiQ9Z0F6
KOiK10994
OMAiTLHCKHG
OrthoDBiEOG091G0B2E
PhylomeDBiQ9Z0F6
TreeFamiTF101212

Family and domain databases

InterProiView protein in InterPro
IPR026584 Rad9
IPR007268 Rad9/Ddc1
PANTHERiPTHR15237 PTHR15237, 1 hit
PfamiView protein in Pfam
PF04139 Rad9, 1 hit
PIRSFiPIRSF009303 Cell_cycle_RAD9, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z0F6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKCLITGGNV KVLGKAVHSL SRIGDELYLE PLKDGLSLRT VNSSRSAYAC
60 70 80 90 100
FLFAPLFFQQ YQAASPGQDL LRCKILMKAF LSVFRSLAIV EKSVEKCCIS
110 120 130 140 150
LSGSHSHLVV QLHCKYGVKK THNLSFQDCE SLQAVFDPAS CPHLLRTPAR
160 170 180 190 200
VLAEAVLSFP LALTEVTLGI GRGRRVILRS YQEEEADSTS KAMVTETSIG
210 220 230 240 250
DEDFQQLHAP EGIAVTFCLK EFRGLLSFAE SANLPLTIHF DVPGRPVIFT
260 270 280 290 300
IEDSLLDAHF VLATLLEQDS CSQGPCSPKP HQPVPQKQAH STPHLDDFTS
310 320 330 340 350
DDIDCYMIAM ETTGGNEGSG AQPSTSLPPV SLASHDLAPT SEEEAEPSTV
360 370 380
PGTPPPKKFR SLFFGSILAP VHSPQGPNPV LAEDSDGEG
Length:389
Mass (Da):42,059
Last modified:May 1, 1999 - v1
Checksum:i2AE8FF75385C668D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045663 mRNA Translation: AAC77365.1
AF045662 Genomic DNA Translation: AAC77364.1
AK134293 mRNA Translation: BAE22086.1
BC082556 mRNA Translation: AAH82556.1
AF469747 Genomic DNA Translation: AAL82448.1
AF469748 Genomic DNA Translation: AAL82449.1
AF469749 Genomic DNA Translation: AAL82450.1
AF469750 Genomic DNA Translation: AAL82451.1
AF469752 Genomic DNA Translation: AAL82453.1
AF469753 Genomic DNA Translation: AAL82454.1
AF469751 Genomic DNA Translation: AAL82452.1
CCDSiCCDS29422.1
RefSeqiNP_035367.1, NM_011237.2
UniGeneiMm.277629

Genome annotation databases

EnsembliENSMUST00000025740; ENSMUSP00000025740; ENSMUSG00000024824
ENSMUST00000181329; ENSMUSP00000137976; ENSMUSG00000097909
GeneIDi19367
KEGGimmu:19367
UCSCiuc008fzh.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045663 mRNA Translation: AAC77365.1
AF045662 Genomic DNA Translation: AAC77364.1
AK134293 mRNA Translation: BAE22086.1
BC082556 mRNA Translation: AAH82556.1
AF469747 Genomic DNA Translation: AAL82448.1
AF469748 Genomic DNA Translation: AAL82449.1
AF469749 Genomic DNA Translation: AAL82450.1
AF469750 Genomic DNA Translation: AAL82451.1
AF469752 Genomic DNA Translation: AAL82453.1
AF469753 Genomic DNA Translation: AAL82454.1
AF469751 Genomic DNA Translation: AAL82452.1
CCDSiCCDS29422.1
RefSeqiNP_035367.1, NM_011237.2
UniGeneiMm.277629

3D structure databases

ProteinModelPortaliQ9Z0F6
SMRiQ9Z0F6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202570, 2 interactors
STRINGi10090.ENSMUSP00000025740

PTM databases

iPTMnetiQ9Z0F6
PhosphoSitePlusiQ9Z0F6

Proteomic databases

EPDiQ9Z0F6
MaxQBiQ9Z0F6
PaxDbiQ9Z0F6
PeptideAtlasiQ9Z0F6
PRIDEiQ9Z0F6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025740; ENSMUSP00000025740; ENSMUSG00000024824
ENSMUST00000181329; ENSMUSP00000137976; ENSMUSG00000097909
GeneIDi19367
KEGGimmu:19367
UCSCiuc008fzh.1 mouse

Organism-specific databases

CTDi5883
MGIiMGI:1328356 Rad9a

Phylogenomic databases

eggNOGiKOG2810 Eukaryota
ENOG410XYYN LUCA
GeneTreeiENSGT00390000005767
HOGENOMiHOG000059650
HOVERGENiHBG058989
InParanoidiQ9Z0F6
KOiK10994
OMAiTLHCKHG
OrthoDBiEOG091G0B2E
PhylomeDBiQ9Z0F6
TreeFamiTF101212

Enzyme and pathway databases

ReactomeiR-MMU-176187 Activation of ATR in response to replication stress
R-MMU-5685938 HDR through Single Strand Annealing (SSA)
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-69473 G2/M DNA damage checkpoint

Miscellaneous databases

ChiTaRSiRad9a mouse
PROiPR:Q9Z0F6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024824 Expressed in 217 organ(s), highest expression level in bone marrow
CleanExiMM_RAD9
GenevisibleiQ9Z0F6 MM

Family and domain databases

InterProiView protein in InterPro
IPR026584 Rad9
IPR007268 Rad9/Ddc1
PANTHERiPTHR15237 PTHR15237, 1 hit
PfamiView protein in Pfam
PF04139 Rad9, 1 hit
PIRSFiPIRSF009303 Cell_cycle_RAD9, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAD9A_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0F6
Secondary accession number(s): Q8QZZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: May 1, 1999
Last modified: November 7, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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