Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calcium and integrin-binding protein 1

Gene

Cib1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-binding protein that plays a role in the regulation of numerous cellular processes, such as cell differentiation, cell division, cell proliferation, cell migration, thrombosis, angiogenesis, cardiac hypertrophy and apoptosis. Involved in bone marrow megakaryocyte differentiation by negatively regulating thrombopoietin-mediated signaling pathway. Participates in the endomitotic cell cycle of megakaryocyte, a form of mitosis in which both karyokinesis and cytokinesis are interrupted. Plays a role in integrin signaling by negatively regulating alpha-IIb/beta3 activation in thrombin-stimulated megakaryocytes preventing platelet aggregation. Up-regulates PTK2/FAK1 activity, and is also needed for the recruitment of PTK2/FAK1 to focal adhesions; it thus appears to play an important role in focal adhesion formation. Positively regulates cell migration on fibronectin in a CDC42-dependent manner, the effect being negatively regulated by PAK1. Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. Down-regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Involved in sphingosine kinase SPHK1 translocation to the plasma membrane in a N-myristoylation-dependent manner preventing TNF-alpha-induced apoptosis. Regulates serine/threonine-protein kinase PLK3 activity for proper completion of cell division progression. Plays a role in microtubule (MT) dynamics during neuronal development; disrupts the MT depolymerization activity of STMN2 attenuating NGF-induced neurite outgrowth and the MT reorganization at the edge of lamellipodia. Promotes cardiomyocyte hypertrophy via activation of the calcineurin/NFAT signaling pathway. Stimulates calcineurin PPP3R1 activity by mediating its anchoring to the sarcolemma. In ischemia-induced (pathological or adaptive) angiogenesis, stimulates endothelial cell proliferation, migration and microvessel formation by activating the PAK1 and ERK1/ERK2 signaling pathway. Promotes also cancer cell survival and proliferation. May regulate cell cycle and differentiation of spermatogenic germ cells, and/or differentiation of supporting Sertoli cells.5 Publications

Miscellaneous

The binding of either calcium or magnesium significantly increases the structural stability of the protein in comparison to apo-CIB (calcium- and magnesium-free form).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi116 – 1271PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi161 – 1722PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium-dependent protein kinase inhibitor activity Source: MGI
  • calcium ion binding Source: HGNC
  • ion channel binding Source: BHF-UCL
  • protein C-terminus binding Source: MGI
  • protein kinase binding Source: MGI
  • protein membrane anchor Source: BHF-UCL
  • protein serine/threonine kinase inhibitor activity Source: MGI
  • Ras GTPase binding Source: MGI

GO - Biological processi

Keywordsi

Biological processAngiogenesis, Apoptosis, Cell adhesion, Cell cycle, Cell division, Differentiation, Spermatogenesis
LigandCalcium, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium and integrin-binding protein 1
Short name:
CIB
Alternative name(s):
Calmyrin
DNA-PKcs-interacting protein
Kinase-interacting protein
Short name:
KIP
Gene namesi
Name:Cib1
Synonyms:Cib, Kip, Prkdcip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1344418 Cib1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice grow normally and are healthy; other family members (CIB2, CIB3 and CIB4) may probably compensate for CIB1 loss (PubMed:18989529). Males are sterile: spermatogenic cells can complete both mitotic and meiotic divisions, but postmeiotic spermatids do not develop normally and sperm is not produced (PubMed:16982698). Display increased bone marrow megakaryocytes and circulating platelets (PubMed:22128142). Mice display tail bleeding time increase, impaired thrombus formation and angiogenesis defect after ischemia (PubMed:19691476). Show compromised tumor growth (PubMed:20804551).5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000735322 – 191Calcium and integrin-binding protein 1Add BLAST190

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

EPDiQ9Z0F4
MaxQBiQ9Z0F4
PaxDbiQ9Z0F4
PeptideAtlasiQ9Z0F4
PRIDEiQ9Z0F4

PTM databases

PhosphoSitePlusiQ9Z0F4

Expressioni

Tissue specificityi

Expressed in testis. Expressed in cardiac myocytes and endothelial cells. Expressed strongly in Sertoli cells, weakly in pachytene spermatocytes, round spermatids and condensing spermatids (at protein level). Ubiquitous.3 Publications

Developmental stagei

Expressed in the heart at 16 dpc (at protein level).1 Publication

Inductioni

Up-regulated upon cardiomyocytes hypertrophy (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000030538 Expressed in 261 organ(s), highest expression level in large intestine
CleanExiMM_CIB1
ExpressionAtlasiQ9Z0F4 baseline and differential
GenevisibleiQ9Z0F4 MM

Interactioni

Subunit structurei

Monomer. Interacts with the heterodimeric integrin alpha-IIb/beta3 (ITGA2B-ITGB3). Interacts with ITGA2B (via cytoplasmic domain); the interaction is direct and calcium-dependent. Interacts with the protein kinases PLK2/SNK and PRKDC (via the region immediately upstream of the kinase domain). Interacts with PLK3; the interaction inhibits PLK3 kinase activity. Interacts with PSEN2. Interacts (via C-terminus) with F8. Interacts with NBR1 (via C-terminus). Interacts with FEZ1 (via C-terminus). Interacts with UBR5 (via C-terminus); the interaction is sensitive to DNA damage, and may target CIB1 for ubiquitin-mediated degradation. Interacts with IFI6; the interaction is direct. Interacts with BCL2. Interacts with TAS1R2 (via C-terminus); the interaction is independent of the myristoylation state of CIB1. Interacts with ITPR3; the interaction occurs in a calcium dependent manner. Interacts with PTK2/FAK1. Interacts with MAP3K5; the interaction inhibits MAP3K5 activation by phosphorylation, and its subsequent interaction with TRAF2. Interacts (via C-terminal region) with STMN2 (via the N-terminal region); the interaction is direct, occurs in a calcium-dependent manner and attenuates the STMN2-induced neurite outgrowth inhibition. Interacts with SPHK1, the interaction occurs in a calcium-dependent manner. Interacts with ITGA2B (via C-terminal cytoplasmic tail); the interaction occurs upon platelet aggregation and is stabilized/increased in a calcium and magnesium-dependent manner. Interacts with PAK1 (via N-terminal region); the interaction is direct and occurs in a calcium-dependent manner. Interacts with RAC3 (via C-terminal region); the interaction induces their association with the cytoskeleton upon alpha-IIb/beta3 integrin-mediated adhesion. Interacts with ITGA5 and ITGAV (By similarity). Interacts with MYO1C. Interacts with ITGA2B (via C-terminal cytoplasmic tail region). Interacts (via C-terminal region) with PPP3R1 isoform 1 and isoform 2; the interactions increase upon cardiomyocytes hypertrophy. Interacts with CACNA1C; the interaction increases upon cardiomyocytes hypertrophy.By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070901

Structurei

3D structure databases

ProteinModelPortaliQ9Z0F4
SMRiQ9Z0F4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini103 – 138EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini148 – 183EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Domaini

The EF-hands may also bind magnesium ions in the presence of high Mg2+ levels and low Ca2+ levels.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0034 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00860000133737
HOGENOMiHOG000233019
HOVERGENiHBG107344
InParanoidiQ9Z0F4
KOiK17259
OMAiQDGDCTF
OrthoDBiEOG091G0OJZ
PhylomeDBiQ9Z0F4
TreeFamiTF313865

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

Q9Z0F4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGGSGSRLSK ELLAEYQDLT FLTKQEILLA HRRFCELLPP EQRTVEESLH
60 70 80 90 100
TRVSFEQILS LPELKANPFK ERICMVFSTS PTRDSLSFED FLDLLSVFSD
110 120 130 140 150
TATPDIKSHY AFRIFDFDDD GTLDREDLSQ LVNCLTGEGE DTRLSASEMK
160 170 180 190
QLIDNILEES DIDRDGTINL SEFQHVISRS PDFASSFKIV L
Length:191
Mass (Da):21,763
Last modified:January 23, 2007 - v3
Checksum:iC85B603A19F9D9AC
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8C2K4Q8C2K4_MOUSE
Calcium and integrin binding 1 (Cal...
Cib1 mCG_19448
143Annotation score:
A0A0U1RPF3A0A0U1RPF3_MOUSE
Calcium and integrin binding 1 (Cal...
Cib1 mCG_19448
186Annotation score:
D3YY46D3YY46_MOUSE
Calcium and integrin-binding protei...
Cib1
113Annotation score:
A0A0U1RPW6A0A0U1RPW6_MOUSE
Calcium and integrin-binding protei...
Cib1
87Annotation score:
A0A0U1RNH3A0A0U1RNH3_MOUSE
Calcium and integrin-binding protei...
Cib1
43Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006463 mRNA Translation: BAA74429.1
AB017361 Genomic DNA Translation: BAA36165.1
AF173010 mRNA Translation: AAG38960.1
AK010345 mRNA Translation: BAB26868.1
AK027915 mRNA Translation: BAC25663.1
AK152805 mRNA Translation: BAE31510.1
AK165472 mRNA Translation: BAE38209.1
BC003714 mRNA Translation: AAH03714.1
BC054385 mRNA Translation: AAH54385.1
CCDSiCCDS21392.1
RefSeqiNP_001278204.1, NM_001291275.1
NP_001278205.1, NM_001291276.1
NP_036000.1, NM_011870.5
UniGeneiMm.30217

Genome annotation databases

EnsembliENSMUST00000065163; ENSMUSP00000070901; ENSMUSG00000030538
GeneIDi23991
KEGGimmu:23991
UCSCiuc009hzs.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006463 mRNA Translation: BAA74429.1
AB017361 Genomic DNA Translation: BAA36165.1
AF173010 mRNA Translation: AAG38960.1
AK010345 mRNA Translation: BAB26868.1
AK027915 mRNA Translation: BAC25663.1
AK152805 mRNA Translation: BAE31510.1
AK165472 mRNA Translation: BAE38209.1
BC003714 mRNA Translation: AAH03714.1
BC054385 mRNA Translation: AAH54385.1
CCDSiCCDS21392.1
RefSeqiNP_001278204.1, NM_001291275.1
NP_001278205.1, NM_001291276.1
NP_036000.1, NM_011870.5
UniGeneiMm.30217

3D structure databases

ProteinModelPortaliQ9Z0F4
SMRiQ9Z0F4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070901

PTM databases

PhosphoSitePlusiQ9Z0F4

Proteomic databases

EPDiQ9Z0F4
MaxQBiQ9Z0F4
PaxDbiQ9Z0F4
PeptideAtlasiQ9Z0F4
PRIDEiQ9Z0F4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065163; ENSMUSP00000070901; ENSMUSG00000030538
GeneIDi23991
KEGGimmu:23991
UCSCiuc009hzs.2 mouse

Organism-specific databases

CTDi10519
MGIiMGI:1344418 Cib1

Phylogenomic databases

eggNOGiKOG0034 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00860000133737
HOGENOMiHOG000233019
HOVERGENiHBG107344
InParanoidiQ9Z0F4
KOiK17259
OMAiQDGDCTF
OrthoDBiEOG091G0OJZ
PhylomeDBiQ9Z0F4
TreeFamiTF313865

Miscellaneous databases

PROiPR:Q9Z0F4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030538 Expressed in 261 organ(s), highest expression level in large intestine
CleanExiMM_CIB1
ExpressionAtlasiQ9Z0F4 baseline and differential
GenevisibleiQ9Z0F4 MM

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCIB1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0F4
Secondary accession number(s): Q3TN80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 23, 2007
Last modified: October 10, 2018
This is version 155 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again