UniProtKB - Q9Z0D9 (CX3C1_MOUSE)
Protein
CX3C chemokine receptor 1
Gene
Cx3cr1
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor for the CX3C chemokine fractalkine (CX3CL1); binds to CX3CL1 and mediates both its adhesive and migratory functions.1 Publication
GO - Molecular functioni
- C-C chemokine binding Source: GO_Central
- C-C chemokine receptor activity Source: GO_Central
- chemokine binding Source: GO_Central
- chemokine receptor activity Source: MGI
- C-X3-C chemokine binding Source: ARUK-UCL
- C-X3-C chemokine receptor activity Source: MGI
- cytokine receptor activity Source: MGI
- G protein-coupled receptor activity Source: ARUK-UCL
GO - Biological processi
- autocrine signaling Source: MGI
- calcium-mediated signaling Source: GO_Central
- cell adhesion Source: ARUK-UCL
- cell chemotaxis Source: MGI
- cellular response to lipopolysaccharide Source: ARUK-UCL
- cellular response to transforming growth factor beta stimulus Source: Ensembl
- central nervous system maturation Source: ARUK-UCL
- cerebral cortex cell migration Source: MGI
- chemokine-mediated signaling pathway Source: MGI
- chemotaxis Source: MGI
- cytokine-mediated signaling pathway Source: MGI
- immune response Source: GO_Central
- innate immune response Source: ARUK-UCL
- leukocyte tethering or rolling Source: ARUK-UCL
- macrophage chemotaxis Source: MGI
- memory Source: MGI
- microglial cell activation Source: MGI
- microglial cell activation involved in immune response Source: MGI
- modulation of chemical synaptic transmission Source: ARUK-UCL
- multiple spine synapse organization, single dendrite Source: ARUK-UCL
- negative regulation of angiogenesis Source: MGI
- negative regulation of apoptotic signaling pathway Source: MGI
- negative regulation of cell migration Source: MGI
- negative regulation of chronic inflammatory response to non-antigenic stimulus Source: MGI
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- negative regulation of hippocampal neuron apoptotic process Source: MGI
- negative regulation of interleukin-1 beta production Source: MGI
- negative regulation of long-term synaptic potentiation Source: MGI
- phospholipase C-activating G protein-coupled receptor signaling pathway Source: ARUK-UCL
- positive regulation of angiogenesis Source: MGI
- positive regulation of cytosolic calcium ion concentration Source: GO_Central
- positive regulation of I-kappaB phosphorylation Source: MGI
- positive regulation of microglial cell migration Source: MGI
- positive regulation of neuroblast proliferation Source: MGI
- positive regulation of neurogenesis Source: ARUK-UCL
- positive regulation of NF-kappaB transcription factor activity Source: MGI
- positive regulation of protein kinase B signaling Source: MGI
- regulation of nitric oxide biosynthetic process Source: ARUK-UCL
- regulation of tumor necrosis factor biosynthetic process Source: ARUK-UCL
- response to ischemia Source: MGI
- social behavior Source: ARUK-UCL
- synapse maturation Source: ARUK-UCL
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-MMU-380108, Chemokine receptors bind chemokines R-MMU-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: CX3C chemokine receptor 1Short name: C-X3-C CKR-1 Short name: CX3CR1 Alternative name(s): Fractalkine receptor |
Gene namesi | Name:Cx3cr1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1333815, Cx3cr1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- external side of plasma membrane Source: GO_Central
- neuronal cell body membrane Source: MGI
Other locations
- cell surface Source: ARUK-UCL
- dendritic tree Source: MGI
- integral component of membrane Source: UniProtKB-KW
- neuron projection Source: MGI
- perinuclear region of cytoplasm Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 32 | ExtracellularSequence analysisAdd BLAST | 32 | |
Transmembranei | 33 – 60 | Helical; Name=1Sequence analysisAdd BLAST | 28 | |
Topological domaini | 61 – 70 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 71 – 91 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 92 – 104 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 105 – 126 | Helical; Name=3Sequence analysisAdd BLAST | 22 | |
Topological domaini | 127 – 143 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 144 – 168 | Helical; Name=4Sequence analysisAdd BLAST | 25 | |
Topological domaini | 169 – 196 | ExtracellularSequence analysisAdd BLAST | 28 | |
Transmembranei | 197 – 216 | Helical; Name=5Sequence analysisAdd BLAST | 20 | |
Topological domaini | 217 – 232 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 233 – 257 | Helical; Name=6Sequence analysisAdd BLAST | 25 | |
Topological domaini | 258 – 274 | ExtracellularSequence analysisAdd BLAST | 17 | |
Transmembranei | 275 – 298 | Helical; Name=7Sequence analysisAdd BLAST | 24 | |
Topological domaini | 299 – 354 | CytoplasmicSequence analysisAdd BLAST | 56 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069327 | 1 – 354 | CX3C chemokine receptor 1Add BLAST | 354 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 103 ↔ 176 | PROSITE-ProRule annotation | ||
Modified residuei | 345 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
This protein is not N-glycosylated which is unusual for G-protein-coupled receptors.By similarity
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
PaxDbi | Q9Z0D9 |
PRIDEi | Q9Z0D9 |
PTM databases
iPTMneti | Q9Z0D9 |
PhosphoSitePlusi | Q9Z0D9 |
SwissPalmi | Q9Z0D9 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000052336, Expressed in brain blood vessel and 227 other tissues |
ExpressionAtlasi | Q9Z0D9, baseline and differential |
Genevisiblei | Q9Z0D9, MM |
Interactioni
Subunit structurei
Found in a ternary complex with CX3CL1 and ITGAV:ITGB3 or ITGA4:ITGB1.
By similarityGO - Molecular functioni
- C-C chemokine binding Source: GO_Central
- chemokine binding Source: GO_Central
- C-X3-C chemokine binding Source: ARUK-UCL
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000063986 |
Miscellaneous databases
RNActi | Q9Z0D9, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QVQK, Eukaryota |
GeneTreei | ENSGT01000000214577 |
HOGENOMi | CLU_009579_8_3_1 |
InParanoidi | Q9Z0D9 |
OMAi | SNFTHYT |
OrthoDBi | 900867at2759 |
PhylomeDBi | Q9Z0D9 |
TreeFami | TF330966 |
Family and domain databases
InterProi | View protein in InterPro IPR005387, Chemokine_CX3CR1 IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01562, FRACTALKINER PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9Z0D9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTSFPELDL ENFEYDDSAE ACYLGDIVAF GTIFLSVFYA LVFTFGLVGN
60 70 80 90 100
LLVVLALTNS RKPKSITDIY LLNLALSDLL FVATLPFWTH YLISHEGLHN
110 120 130 140 150
AMCKLTTAFF FIGFFGGIFF ITVISIDRYL AIVLAANSMN NRTVQHGVTI
160 170 180 190 200
SLGVWAAAIL VASPQFMFTK RKDNECLGDY PEVLQEMWPV LRNSEVNILG
210 220 230 240 250
FALPLLIMSF CYFRIIQTLF SCKNRKKARA VRLILLVVFA FFLFWTPYNI
260 270 280 290 300
MIFLETLKFY NFFPSCDMKR DLRLALSVTE TVAFSHCCLN PFIYAFAGEK
310 320 330 340 350
FRRYLGHLYR KCLAVLCGHP VHTGFSPESQ RSRQDSILSS FTHYTSEGDG
SLLL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF074912 Genomic DNA Translation: AAD08665.1 AF102269 mRNA Translation: AAC72408.1 BC012653 mRNA Translation: AAH12653.1 |
CCDSi | CCDS40806.1 |
RefSeqi | NP_034117.3, NM_009987.4 |
Genome annotation databases
Ensembli | ENSMUST00000064165; ENSMUSP00000063986; ENSMUSG00000052336 ENSMUST00000177637; ENSMUSP00000136413; ENSMUSG00000052336 ENSMUST00000215016; ENSMUSP00000150463; ENSMUSG00000052336 |
GeneIDi | 13051 |
KEGGi | mmu:13051 |
UCSCi | uc009sbz.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF074912 Genomic DNA Translation: AAD08665.1 AF102269 mRNA Translation: AAC72408.1 BC012653 mRNA Translation: AAH12653.1 |
CCDSi | CCDS40806.1 |
RefSeqi | NP_034117.3, NM_009987.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000063986 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
iPTMneti | Q9Z0D9 |
PhosphoSitePlusi | Q9Z0D9 |
SwissPalmi | Q9Z0D9 |
Proteomic databases
PaxDbi | Q9Z0D9 |
PRIDEi | Q9Z0D9 |
Protocols and materials databases
Antibodypediai | 6554, 734 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000064165; ENSMUSP00000063986; ENSMUSG00000052336 ENSMUST00000177637; ENSMUSP00000136413; ENSMUSG00000052336 ENSMUST00000215016; ENSMUSP00000150463; ENSMUSG00000052336 |
GeneIDi | 13051 |
KEGGi | mmu:13051 |
UCSCi | uc009sbz.2, mouse |
Organism-specific databases
CTDi | 1524 |
MGIi | MGI:1333815, Cx3cr1 |
Phylogenomic databases
eggNOGi | ENOG502QVQK, Eukaryota |
GeneTreei | ENSGT01000000214577 |
HOGENOMi | CLU_009579_8_3_1 |
InParanoidi | Q9Z0D9 |
OMAi | SNFTHYT |
OrthoDBi | 900867at2759 |
PhylomeDBi | Q9Z0D9 |
TreeFami | TF330966 |
Enzyme and pathway databases
Reactomei | R-MMU-380108, Chemokine receptors bind chemokines R-MMU-418594, G alpha (i) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 13051, 2 hits in 17 CRISPR screens |
ChiTaRSi | Cx3cr1, mouse |
PROi | PR:Q9Z0D9 |
RNActi | Q9Z0D9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000052336, Expressed in brain blood vessel and 227 other tissues |
ExpressionAtlasi | Q9Z0D9, baseline and differential |
Genevisiblei | Q9Z0D9, MM |
Family and domain databases
InterProi | View protein in InterPro IPR005387, Chemokine_CX3CR1 IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01562, FRACTALKINER PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CX3C1_MOUSE | |
Accessioni | Q9Z0D9Primary (citable) accession number: Q9Z0D9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries