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Entry version 75 (31 Jul 2019)
Sequence version 1 (01 May 1999)
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Protein

Replicase large subunit

Gene
N/A
Organism
Tobacco mosaic virus (strain B935A) (TMV)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Replicase large subunit: is an RNA-dependent RNA polymerase active in viral RNA replication.By similarity
Replicase small subunit: is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Helicase region probably exhibits NTPase and RNA unwinding activities (Potential). It also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May mediate silencing suppression through either inhibition of HEN1-mediated siRNA or siRNA demethylation (By similarity).By similarityCurated

Miscellaneous

This protein is translated as a fusion protein by episodic readthrough of a termination codon. When readthrough of the terminator codon TGA occurs between the codons for Gln-1116 and Gln-1118, this results in the addition of the RdRp region to the replicase.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi833 – 840ATPSequence analysis8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHelicase, Hydrolase, Nucleotidyltransferase, RNA-directed RNA polymerase, Suppressor of RNA silencing, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Replicase large subunit (EC:2.1.1.-, EC:2.7.7.-, EC:2.7.7.48, EC:3.6.4.13)
Alternative name(s):
183 kDa protein
RNA-directed RNA polymerase
Cleaved into the following chain:
Replicase small subunit (EC:2.1.1.-, EC:2.7.7.-, EC:3.6.4.13)
Alternative name(s):
126 kDa protein
Methyltransferase/RNA helicase
Short name:
MT/HEL
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiTobacco mosaic virus (strain B935A) (TMV)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri138309 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaVirgaviridaeTobamovirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiNicotiana tabacum (Common tobacco) [TaxID: 4097]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000279 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000411641 – 1616Replicase large subunitAdd BLAST1616
ChainiPRO_00000411651 – 1116Replicase small subunitAdd BLAST1116

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterodimer of a large and a small subunit.

By similarity

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini72 – 281Alphavirus-like MTPROSITE-ProRule annotationAdd BLAST210
Domaini801 – 963(+)RNA virus helicase ATP-bindingAdd BLAST163
Domaini964 – 1116(+)RNA virus helicase C-terminalAdd BLAST153
Domaini1380 – 1493RdRp catalyticPROSITE-ProRule annotationAdd BLAST114

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni50 – 458MethyltransferaseSequence analysisAdd BLAST409
Regioni830 – 1085HelicaseSequence analysisAdd BLAST256

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR027351 (+)RNA_virus_helicase_core_dom
IPR002588 Alphavirus-like_MT_dom
IPR027417 P-loop_NTPase
IPR007094 RNA-dir_pol_PSvirus
IPR001788 Tymovirus_RNA-dep_RNA_pol

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00978 RdRP_2, 1 hit
PF01443 Viral_helicase1, 1 hit
PF01660 Vmethyltransf, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52540 SSF52540, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51743 ALPHAVIRUS_MT, 1 hit
PS51657 PSRV_HELICASE, 1 hit
PS50507 RDRP_SSRNA_POS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

Q9YQ08-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAYTQTATTS ALLDTVRGNN SLVNDLAKRR LYDTAVEEFN ARDRRPKVNF
60 70 80 90 100
SKVISEEQTL IATRAYPEFQ ITFYNTQNAV HSLAGGLRSL ELEYLMMQIP
110 120 130 140 150
YGSLTYDIGG NFASHLFKGR AYVHCCMPNL DVRDIMRHEG QKDSIELYLS
160 170 180 190 200
RLERGGKTVP NFQKEAFDRY AEIPEDAVCH NTFQTCEHQP MQQSGRVYAI
210 220 230 240 250
ALHSIYDIPA DEFGAALLRK NVHTCYAAFH FSENLLLEDS YVNLDEINAC
260 270 280 290 300
FSRDGDKLTF SFASESTLNY CHSYSNILKY VCKTYFPASN REVYMKEFLV
310 320 330 340 350
TRVNTWFCKF SRIDTFLLYK GVAHKSVDSE QFYTAMEDAW HYKKTLAMCN
360 370 380 390 400
SERILLEDSS SVNYWFPKMR DMVIVPLFDI SLETSKRTRK EVLVSKDFVF
410 420 430 440 450
TVLNHIRTYQ AKALTYANVL SFVESIRSRV IINGVTARSE WDVDKSLLQS
460 470 480 490 500
LSMTFYLHTK LAVLKDDLLI SKFSLGSKTV CQHVWDEISL AFGNAFPSVK
510 520 530 540 550
ERLLNRKLIR VAGDALEIRV PDLYVTFHDR LVTEYKASVD MPALDIRKKM
560 570 580 590 600
EETEVMYNAL SELSVLRESD KFDVDVFSQM CKSLEVDPMT AAKVIVADMS
610 620 630 640 650
NESGLTLTFE RPTEANVALA LQDQEKASEG ALVVTSREVE EPSMKGSMAR
660 670 680 690 700
GELQLAGLAG DHPESSYSKN EEIESLEQFH MATADSLIRK QMSSIVYTGP
710 720 730 740 750
IKVQQMKNFI DSLVASLSAA VSNLVKILKD TAAIDLETRQ KFGVLDVASR
760 770 780 790 800
KWLIKPTAKS HAWGVVETHA RKYHVALLEY DEQGVVTCDD WRRVAVSSES
810 820 830 840 850
VVYSDMAKLR TLRRLLRNGE PHVSSAKVVL VDGVPGCGKT KEILSRVNFD
860 870 880 890 900
EDLILVPGKQ AAEMIRRRAN SSGIIVATKD NVKTVDSFMM NFGKSTRCQF
910 920 930 940 950
KRLFIDEGLM LHTGCVNFLV AMSLCEIAYV YGDTQQIPYI NRVSGFPYPA
960 970 980 990 1000
HFAKLEVDEV ETRRTTLRCP ADVTHYLNRR YEGFVMSTSS VKKSVSQEMV
1010 1020 1030 1040 1050
GGAAVINPIS KPSHGKILTF TQSDKEALLS RGYSDVHTVH EVQGETYSDV
1060 1070 1080 1090 1100
SLVRLTPTPV SIIAGDSPHV LVALSRHTCS LKYYTVVMDP LVSIIRDLEK
1110 1120 1130 1140 1150
LSSYLSDMYK VDAGTQXQLQ IDSVFKGSNL FVAAPKTGDI SDMQFYYDKC
1160 1170 1180 1190 1200
LPGNSTMMNN FDAVTMRLTD ISLNVKDCIL DMSKSVAAPK DQIKPLIPMV
1210 1220 1230 1240 1250
RTAAEMPRQT GLLENLVAMI KRNFNAPELS GIIDIENTAS LVVDKFFDSY
1260 1270 1280 1290 1300
LLKEKRKPNK NVSLFSRESL NRWLEKQEQV TIGQLADFDF VDLPAVDQYR
1310 1320 1330 1340 1350
HMIKAQPKQK LDTSIQTEYP ALQTIVYHSK KISAIFGPLF SELTRQLLDS
1360 1370 1380 1390 1400
VDSSRFLFFT RKTPAQIEDF FGDLDSHVPM DVLELDISKY DKSQNEFHCA
1410 1420 1430 1440 1450
VEYEIWRRLG FEDFLGEVWK QGHRKTTLKD YTAGIKTCIW YQRKSGDVTT
1460 1470 1480 1490 1500
FIGNTVIIAA CLASMLPMEK IIKGAFCGDD SLLYFPKGCE FPDVQHSANL
1510 1520 1530 1540 1550
MWNFEAKLFK KQYGYFCGRY VIHHDRGCIV YYDPLKLISK LGAKHIKDWE
1560 1570 1580 1590 1600
HLEEFRRSLC DVAVSLNNCA YYTQLDDAVW EVHKTAPPGS FVYKSLVKYL
1610
SDKVLFRSLF IDGSSC
Length:1,616
Mass (Da):183,299
Last modified:May 1, 1999 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i99A63B441290E6CF
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AJ011933 Genomic RNA Translation: CAA09875.1
AJ011933 Genomic RNA Translation: CAA09874.1

Keywords - Coding sequence diversityi

RNA suppression of termination

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011933 Genomic RNA Translation: CAA09875.1
AJ011933 Genomic RNA Translation: CAA09874.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiView protein in InterPro
IPR027351 (+)RNA_virus_helicase_core_dom
IPR002588 Alphavirus-like_MT_dom
IPR027417 P-loop_NTPase
IPR007094 RNA-dir_pol_PSvirus
IPR001788 Tymovirus_RNA-dep_RNA_pol
PfamiView protein in Pfam
PF00978 RdRP_2, 1 hit
PF01443 Viral_helicase1, 1 hit
PF01660 Vmethyltransf, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51743 ALPHAVIRUS_MT, 1 hit
PS51657 PSRV_HELICASE, 1 hit
PS50507 RDRP_SSRNA_POS, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRDRP_TMVB
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9YQ08
Secondary accession number(s): Q9WKD4
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: July 31, 2019
This is version 75 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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