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Entry version 116 (07 Apr 2021)
Sequence version 1 (01 Nov 1999)
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Protein

Bifunctional cytochrome P450/NADPH--P450 reductase

Gene

CYP505

Organism
Fusarium oxysporum (Fusarium vascular wilt)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Functions as a fatty acid monooxygenase (PubMed:8830036, PubMed:11985584). Catalyzes hydroxylation of fatty acids at omega-1, omega-2 and omega-3 positions (PubMed:11985584). Shows activity toward fatty acids with a chain length of 9-18 carbons with optimum chain lengths of 12-14 carbons (lauric, tridecylic and myristic acids) (PubMed:8830036, PubMed:11985584). Can also use shorter saturated fatty acids with a chain length of 9 or 10 carbons as substrates (PubMed:11985584). Also displays a NADPH-dependent reductase activity in the C-terminal domain, which allows electron transfer from NADPH to the heme iron of the cytochrome P450 N-terminal domain (PubMed:8830036, PubMed:11985584).2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Stimulated NADPH--cytochrome reductase activity in the presence of substrate. Inhibited by fatty acid substrates longer than 13 carbons and the degree of inhibition increases with increasing chain length.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.16 mM for NADH1 Publication
  2. KM=0.15 mM for laurate1 Publication
  3. KM=3200 µM for nonanoic acid1 Publication
  4. KM=260 µM for decanoic acid1 Publication
  5. KM=160 µM for undecanoic acid1 Publication
  6. KM=30 µM for laurate/dodecanoic acid1 Publication
  7. KM=36 µM for tridecanoic acid1 Publication
  8. KM=19 µM for tetradecanoic acid1 Publication
  9. KM=8 µM for pentadecanoic acid1 Publication
  10. KM=10 µM for hexadecanoic acid1 Publication
  11. KM=74 µM for NADH1 Publication

    pH dependencei

    Optimum pH is 6.5.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei270Important for catalytic activityBy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi407Iron (heme axial ligand)By similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei588FMNBy similarity1
    Binding sitei596FMNBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi506 – 511FMNBy similarity6
    Nucleotide bindingi554 – 557FMNBy similarity4

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionMonooxygenase, Multifunctional enzyme, Oxidoreductase
    Biological processElectron transport, Transport
    LigandFAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BRENDA Comprehensive Enzyme Information System

    More...
    BRENDAi
    1.11.2.4, 2351

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Bifunctional cytochrome P450/NADPH--P450 reductaseCurated
    Alternative name(s):
    Cytochrome P450foxy1 Publication
    Fatty acid omega-hydroxylase
    P450foxy1 Publication
    Including the following 2 domains:
    Cytochrome P450 505 (EC:1.14.14.12 Publications)
    NADPH--cytochrome P450 reductase (EC:1.6.2.42 Publications)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:CYP5051 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiFusarium oxysporum (Fusarium vascular wilt)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri5507 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium oxysporum species complex

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Keywords - Cellular componenti

    Membrane

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000522101 – 1066Bifunctional cytochrome P450/NADPH--P450 reductaseAdd BLAST1066

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    Q9Y8G7

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini500 – 641Flavodoxin-likePROSITE-ProRule annotationAdd BLAST142
    Domaini676 – 904FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST229

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 480Cytochrome P450Add BLAST480
    Regioni481 – 1066NADPH-P-450 reductaseAdd BLAST586

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    In the N-terminal section; belongs to the cytochrome P450 family.Curated

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    1.10.630.10, 1 hit
    1.20.990.10, 1 hit
    3.40.50.360, 1 hit
    3.40.50.80, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR023206, Bifunctional_P450_P450_red
    IPR003097, CysJ-like_FAD-binding
    IPR001128, Cyt_P450
    IPR017972, Cyt_P450_CS
    IPR002401, Cyt_P450_E_grp-I
    IPR036396, Cyt_P450_sf
    IPR017927, FAD-bd_FR_type
    IPR008254, Flavodoxin/NO_synth
    IPR029039, Flavoprotein-like_sf
    IPR039261, FNR_nucleotide-bd
    IPR023173, NADPH_Cyt_P450_Rdtase_alpha
    IPR001433, OxRdtase_FAD/NAD-bd
    IPR017938, Riboflavin_synthase-like_b-brl

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00667, FAD_binding_1, 1 hit
    PF00258, Flavodoxin_1, 1 hit
    PF00175, NAD_binding_1, 1 hit
    PF00067, p450, 1 hit

    PIRSF; a whole-protein classification database

    More...
    PIRSFi
    PIRSF000209, Bifunctional_P450_P450R, 1 hit

    Protein Motif fingerprint database; a protein domain database

    More...
    PRINTSi
    PR00463, EP450I
    PR00385, P450

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF48264, SSF48264, 1 hit
    SSF52218, SSF52218, 1 hit
    SSF52343, SSF52343, 1 hit
    SSF63380, SSF63380, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS00086, CYTOCHROME_P450, 1 hit
    PS51384, FAD_FR, 1 hit
    PS50902, FLAVODOXIN_LIKE, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    Q9Y8G7-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MAESVPIPEP PGYPLIGNLG EFTSNPLSDL NRLADTYGPI FRLRLGAKAP
    60 70 80 90 100
    IFVSSNSLIN EVCDEKRFKK TLKSVLSQVR EGVHDGLFTA FEDEPNWGKA
    110 120 130 140 150
    HRILVPAFGP LSIRGMFPEM HDIATQLCMK FARHGPRTPI DTSDNFTRLA
    160 170 180 190 200
    LDTLALCAMD FRFYSYYKEE LHPFIEAMGD FLTESGNRNR RPPFAPNFLY
    210 220 230 240 250
    RAANEKFYGD IALMKSVADE VVAARKASPS DRKDLLAAML NGVDPQTGEK
    260 270 280 290 300
    LSDENITNQL ITFLIAGHET TSGTLSFAMY QLLKNPEAYS KVQKEVDEVV
    310 320 330 340 350
    GRGPVLVEHL TKLPYISAVL RETLRLNSPI TAFGLEAIDD TFLGGKYLVK
    360 370 380 390 400
    KGEIVTALLS RGHVDPVVYG NDADKFIPER MLDDEFARLN KEYPNCWKPF
    410 420 430 440 450
    GNGKRACIGR PFAWQESLLA MVVLFQNFNF TMTDPNYALE IKQTLTIKPD
    460 470 480 490 500
    HFYINATLRH GMTPTELEHV LAGNGATSSS THNIKAAANL DAKAGSGKPM
    510 520 530 540 550
    AIFYGSNSGT CEALANRLAS DAPSHGFSAT TVGPLDQAKQ NLPEDRPVVI
    560 570 580 590 600
    VTASYEGQPP SNAAHFIKWM EDLDGNDMEK VSYAVFACGH HDWVETFHRI
    610 620 630 640 650
    PKLVDSTLEK RGGTRLVPMG SADAATSDMF SDFEAWEDIV LWPGLKEKYK
    660 670 680 690 700
    ISDEESGGQK GLLVEVSTPR KTSLRQDVEE ALVVAEKTLT KSGPAKKHIE
    710 720 730 740 750
    IQLPSAMTYK AGDYLAILPL NPKSTVARVF RRFSLAWDSF LKIQSEGPTT
    760 770 780 790 800
    LPTNVAISAF DVFSAYVELS QPATKRNILA LAEATEDKDT IQELERLAGD
    810 820 830 840 850
    AYQAEISPKR VSVLDLLEKF PAVALPISSY LAMLPPMRVR QYSISSSPFA
    860 870 880 890 900
    DPSKLTLTYS LLDAPSLSGQ GRHVGVATNF LSHLTAGDKL HVSVRASSEA
    910 920 930 940 950
    FHLPSDAEKT PIICVAAGTG LAPLRGFIQE RAAMLAAGRT LAPALLFFGC
    960 970 980 990 1000
    RNPEIDDLYA EEFERWEKMG AVDVRRAYSR ATDKSEGCKY VQDRVYHDRA
    1010 1020 1030 1040 1050
    DVFKVWDQGA KVFICGSREI GKAVEDVCVR LAIEKAQQNG RDVTEEMARA
    1060
    WFERSRNERF ATDVFD
    Length:1,066
    Mass (Da):117,926
    Last modified:November 1, 1999 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i6B8123698C223DBA
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AB030037 Genomic DNA Translation: BAA82526.1

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB030037 Genomic DNA Translation: BAA82526.1

    3D structure databases

    SMRiQ9Y8G7
    ModBaseiSearch...

    Enzyme and pathway databases

    BRENDAi1.11.2.4, 2351

    Family and domain databases

    Gene3Di1.10.630.10, 1 hit
    1.20.990.10, 1 hit
    3.40.50.360, 1 hit
    3.40.50.80, 1 hit
    InterProiView protein in InterPro
    IPR023206, Bifunctional_P450_P450_red
    IPR003097, CysJ-like_FAD-binding
    IPR001128, Cyt_P450
    IPR017972, Cyt_P450_CS
    IPR002401, Cyt_P450_E_grp-I
    IPR036396, Cyt_P450_sf
    IPR017927, FAD-bd_FR_type
    IPR008254, Flavodoxin/NO_synth
    IPR029039, Flavoprotein-like_sf
    IPR039261, FNR_nucleotide-bd
    IPR023173, NADPH_Cyt_P450_Rdtase_alpha
    IPR001433, OxRdtase_FAD/NAD-bd
    IPR017938, Riboflavin_synthase-like_b-brl
    PfamiView protein in Pfam
    PF00667, FAD_binding_1, 1 hit
    PF00258, Flavodoxin_1, 1 hit
    PF00175, NAD_binding_1, 1 hit
    PF00067, p450, 1 hit
    PIRSFiPIRSF000209, Bifunctional_P450_P450R, 1 hit
    PRINTSiPR00463, EP450I
    PR00385, P450
    SUPFAMiSSF48264, SSF48264, 1 hit
    SSF52218, SSF52218, 1 hit
    SSF52343, SSF52343, 1 hit
    SSF63380, SSF63380, 1 hit
    PROSITEiView protein in PROSITE
    PS00086, CYTOCHROME_P450, 1 hit
    PS51384, FAD_FR, 1 hit
    PS50902, FLAVODOXIN_LIKE, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiC505_FUSOX
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9Y8G7
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
    Last sequence update: November 1, 1999
    Last modified: April 7, 2021
    This is version 116 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
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