UniProtKB - Q9Y8G7 (C505_FUSOX)
Bifunctional cytochrome P450/NADPH--P450 reductase
CYP505
Functioni
Functions as a fatty acid monooxygenase (PubMed:8830036, PubMed:11985584).
Catalyzes hydroxylation of fatty acids at omega-1, omega-2 and omega-3 positions (PubMed:11985584).
Shows activity toward fatty acids with a chain length of 9-18 carbons with optimum chain lengths of 12-14 carbons (lauric, tridecylic and myristic acids) (PubMed:8830036, PubMed:11985584).
Can also use shorter saturated fatty acids with a chain length of 9 or 10 carbons as substrates (PubMed:11985584).
Also displays a NADPH-dependent reductase activity in the C-terminal domain, which allows electron transfer from NADPH to the heme iron of the cytochrome P450 N-terminal domain (PubMed:8830036, PubMed:11985584).
2 PublicationsCatalytic activityi
- an organic molecule + O2 + reduced [NADPH—hemoprotein reductase] = an alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]2 PublicationsEC:1.14.14.12 Publications
- EC:1.6.2.42 Publications
Cofactori
Protein has several cofactor binding sites:- FAD1 PublicationNote: Binds 1 FAD.By similarity
- FMN1 PublicationNote: Binds 1 FMN.By similarity
- heme1 Publication
Activity regulationi
Kineticsi
- KM=0.16 mM for NADH1 Publication
- KM=0.15 mM for laurate1 Publication
- KM=3200 µM for nonanoic acid1 Publication
- KM=260 µM for decanoic acid1 Publication
- KM=160 µM for undecanoic acid1 Publication
- KM=30 µM for laurate/dodecanoic acid1 Publication
- KM=36 µM for tridecanoic acid1 Publication
- KM=19 µM for tetradecanoic acid1 Publication
- KM=8 µM for pentadecanoic acid1 Publication
- KM=10 µM for hexadecanoic acid1 Publication
- KM=74 µM for NADH1 Publication
pH dependencei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 270 | Important for catalytic activityBy similarity | 1 | |
Metal bindingi | 407 | Iron (heme axial ligand)By similarity | 1 | |
Binding sitei | 588 | FMNBy similarity | 1 | |
Binding sitei | 596 | FMNBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 506 – 511 | FMNBy similarity | 6 | |
Nucleotide bindingi | 554 – 557 | FMNBy similarity | 4 |
GO - Molecular functioni
- aromatase activity Source: UniProtKB-EC
- FMN binding Source: InterPro
- heme binding Source: InterPro
- iron ion binding Source: UniProtKB
- NADPH-hemoprotein reductase activity Source: UniProtKB-EC
GO - Biological processi
- metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Monooxygenase, Multifunctional enzyme, Oxidoreductase |
Biological process | Electron transport, Transport |
Ligand | FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP |
Enzyme and pathway databases
BRENDAi | 1.11.2.4, 2351 |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional cytochrome P450/NADPH--P450 reductaseCuratedAlternative name(s): Cytochrome P450foxy1 Publication Fatty acid omega-hydroxylase P450foxy1 Publication Including the following 2 domains: |
Gene namesi | Name:CYP5051 Publication |
Organismi | Fusarium oxysporum (Fusarium vascular wilt) |
Taxonomic identifieri | 5507 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Fusarium › Fusarium oxysporum species complex |
Organism-specific databases
VEuPathDBi | FungiDB:FOC1_g10006735 FungiDB:FOC4_g10006868 FungiDB:FOIG_06751 FungiDB:FOMG_03037 FungiDB:FOXG_04152 FungiDB:FOZG_02961 |
Subcellular locationi
Other locations
- Membrane 2 Publications; Peripheral membrane protein 2 Publications
Other locations
- membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000052210 | 1 – 1066 | Bifunctional cytochrome P450/NADPH--P450 reductaseAdd BLAST | 1066 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 500 – 641 | Flavodoxin-likePROSITE-ProRule annotationAdd BLAST | 142 | |
Domaini | 676 – 904 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 229 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 480 | Cytochrome P450Add BLAST | 480 | |
Regioni | 481 – 1066 | NADPH-P-450 reductaseAdd BLAST | 586 |
Sequence similaritiesi
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit 1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR023206, Bifunctional_P450_P450_red IPR003097, CysJ-like_FAD-binding IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf IPR017927, FAD-bd_FR_type IPR008254, Flavodoxin/NO_synth IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF00067, p450, 1 hit |
PIRSFi | PIRSF000209, Bifunctional_P450_P450R, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAESVPIPEP PGYPLIGNLG EFTSNPLSDL NRLADTYGPI FRLRLGAKAP
60 70 80 90 100
IFVSSNSLIN EVCDEKRFKK TLKSVLSQVR EGVHDGLFTA FEDEPNWGKA
110 120 130 140 150
HRILVPAFGP LSIRGMFPEM HDIATQLCMK FARHGPRTPI DTSDNFTRLA
160 170 180 190 200
LDTLALCAMD FRFYSYYKEE LHPFIEAMGD FLTESGNRNR RPPFAPNFLY
210 220 230 240 250
RAANEKFYGD IALMKSVADE VVAARKASPS DRKDLLAAML NGVDPQTGEK
260 270 280 290 300
LSDENITNQL ITFLIAGHET TSGTLSFAMY QLLKNPEAYS KVQKEVDEVV
310 320 330 340 350
GRGPVLVEHL TKLPYISAVL RETLRLNSPI TAFGLEAIDD TFLGGKYLVK
360 370 380 390 400
KGEIVTALLS RGHVDPVVYG NDADKFIPER MLDDEFARLN KEYPNCWKPF
410 420 430 440 450
GNGKRACIGR PFAWQESLLA MVVLFQNFNF TMTDPNYALE IKQTLTIKPD
460 470 480 490 500
HFYINATLRH GMTPTELEHV LAGNGATSSS THNIKAAANL DAKAGSGKPM
510 520 530 540 550
AIFYGSNSGT CEALANRLAS DAPSHGFSAT TVGPLDQAKQ NLPEDRPVVI
560 570 580 590 600
VTASYEGQPP SNAAHFIKWM EDLDGNDMEK VSYAVFACGH HDWVETFHRI
610 620 630 640 650
PKLVDSTLEK RGGTRLVPMG SADAATSDMF SDFEAWEDIV LWPGLKEKYK
660 670 680 690 700
ISDEESGGQK GLLVEVSTPR KTSLRQDVEE ALVVAEKTLT KSGPAKKHIE
710 720 730 740 750
IQLPSAMTYK AGDYLAILPL NPKSTVARVF RRFSLAWDSF LKIQSEGPTT
760 770 780 790 800
LPTNVAISAF DVFSAYVELS QPATKRNILA LAEATEDKDT IQELERLAGD
810 820 830 840 850
AYQAEISPKR VSVLDLLEKF PAVALPISSY LAMLPPMRVR QYSISSSPFA
860 870 880 890 900
DPSKLTLTYS LLDAPSLSGQ GRHVGVATNF LSHLTAGDKL HVSVRASSEA
910 920 930 940 950
FHLPSDAEKT PIICVAAGTG LAPLRGFIQE RAAMLAAGRT LAPALLFFGC
960 970 980 990 1000
RNPEIDDLYA EEFERWEKMG AVDVRRAYSR ATDKSEGCKY VQDRVYHDRA
1010 1020 1030 1040 1050
DVFKVWDQGA KVFICGSREI GKAVEDVCVR LAIEKAQQNG RDVTEEMARA
1060
WFERSRNERF ATDVFD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB030037 Genomic DNA Translation: BAA82526.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB030037 Genomic DNA Translation: BAA82526.1 |
3D structure databases
AlphaFoldDBi | Q9Y8G7 |
SMRi | Q9Y8G7 |
ModBasei | Search... |
Organism-specific databases
VEuPathDBi | FungiDB:FOC1_g10006735 FungiDB:FOC4_g10006868 FungiDB:FOIG_06751 FungiDB:FOMG_03037 FungiDB:FOXG_04152 FungiDB:FOZG_02961 |
Enzyme and pathway databases
BRENDAi | 1.11.2.4, 2351 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit 1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR023206, Bifunctional_P450_P450_red IPR003097, CysJ-like_FAD-binding IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf IPR017927, FAD-bd_FR_type IPR008254, Flavodoxin/NO_synth IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF00067, p450, 1 hit |
PIRSFi | PIRSF000209, Bifunctional_P450_P450R, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | C505_FUSOX | |
Accessioni | Q9Y8G7Primary (citable) accession number: Q9Y8G7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2002 |
Last sequence update: | November 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families