UniProtKB - Q9Y6W5 (WASF2_HUMAN)
Wiskott-Aldrich syndrome protein family member 2
WASF2
Functioni
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
2 PublicationsGO - Molecular functioni
- actin binding Source: ProtInc
- Arp2/3 complex binding Source: GO_Central
- cadherin binding Source: BHF-UCL
- protein kinase A binding Source: UniProtKB
- protein kinase A regulatory subunit binding Source: GO_Central
- SH3 domain binding Source: UniProtKB
GO - Biological processi
- actin cytoskeleton organization Source: GO_Central
- actin filament-based movement Source: Ensembl
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: ProtInc
- ameboidal-type cell migration Source: Ensembl
- angiogenesis Source: Ensembl
- endocytosis Source: Ensembl
- lamellipodium assembly Source: Ensembl
- lamellipodium morphogenesis Source: Ensembl
- megakaryocyte development Source: Ensembl
- negative regulation of stress fiber assembly Source: UniProtKB
- positive regulation of Arp2/3 complex-mediated actin nucleation Source: GO_Central
- positive regulation of lamellipodium assembly Source: UniProtKB
- postsynaptic actin cytoskeleton organization Source: Ensembl
- Rac protein signal transduction Source: Ensembl
Keywordsi
Molecular function | Actin-binding |
Biological process | Host-virus interaction |
Enzyme and pathway databases
PathwayCommonsi | Q9Y6W5 |
Reactomei | R-HSA-2029482, Regulation of actin dynamics for phagocytic cup formation R-HSA-4420097, VEGFA-VEGFR2 Pathway R-HSA-5663213, RHO GTPases Activate WASPs and WAVEs R-HSA-9013149, RAC1 GTPase cycle R-HSA-9013404, RAC2 GTPase cycle R-HSA-9013423, RAC3 GTPase cycle R-HSA-9664422, FCGR3A-mediated phagocytosis |
SignaLinki | Q9Y6W5 |
SIGNORi | Q9Y6W5 |
Names & Taxonomyi
Protein namesi | Recommended name: Wiskott-Aldrich syndrome protein family member 2Short name: WASP family protein member 2 Alternative name(s): Protein WAVE-2 Verprolin homology domain-containing protein 2 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12733, WASF2 |
MIMi | 605875, gene |
neXtProti | NX_Q9Y6W5 |
VEuPathDBi | HostDB:ENSG00000158195 |
Subcellular locationi
Plasma membrane
- Basolateral cell membrane 1 Publication
Cytoskeleton
- cytoskeleton By similarity
Other locations
- lamellipodium By similarity
Note: At the interface between the lamellipodial actin meshwork and the membrane.By similarity
Cytoskeleton
- actin cytoskeleton Source: ProtInc
Cytosol
- cytosol Source: Reactome
Endosome
- early endosome Source: Ensembl
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- basolateral plasma membrane Source: UniProtKB-SubCell
Other locations
- cell-cell junction Source: Ensembl
- lamellipodium Source: MGI
- protein-containing complex Source: UniProtKB
- ruffle Source: Ensembl
- SCAR complex Source: UniProtKB
- synapse Source: Ensembl
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 40 | L → D: Decreased interaction with WAVE complex; when associated with D-51. 1 Publication | 1 | |
Mutagenesisi | 50 | I → D: Decreased interaction with WAVE complex; when associated with D-54. 1 Publication | 1 | |
Mutagenesisi | 51 | F → D: Decreased interaction with WAVE complex; when associated with D-40. 1 Publication | 1 | |
Mutagenesisi | 54 | L → D: Decreased interaction with WAVE complex; when associated with D-50. 1 Publication | 1 | |
Mutagenesisi | 124 | Y → D: Constitutive induction of the formation of actin filaments. Strongly enhances formation of lamellipodia. 1 Publication | 1 | |
Mutagenesisi | 150 | Y → D: Constitutive induction of the formation of actin filaments. Strongly enhances formation of lamellipodia. 1 Publication | 1 | |
Mutagenesisi | 160 – 161 | WK → ED: Constitutive induction of the formation of actin filaments. Strongly enhances formation of lamellipodia. 1 Publication | 2 |
Organism-specific databases
DisGeNETi | 10163 |
OpenTargetsi | ENSG00000158195 |
PharmGKBi | PA37344 |
Miscellaneous databases
Pharosi | Q9Y6W5, Tbio |
Genetic variation databases
BioMutai | WASF2 |
DMDMi | 59800456 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000188994 | 1 – 498 | Wiskott-Aldrich syndrome protein family member 2Add BLAST | 498 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 474 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9Y6W5 |
jPOSTi | Q9Y6W5 |
MassIVEi | Q9Y6W5 |
MaxQBi | Q9Y6W5 |
PaxDbi | Q9Y6W5 |
PeptideAtlasi | Q9Y6W5 |
PRIDEi | Q9Y6W5 |
ProteomicsDBi | 86801 [Q9Y6W5-1] 86802 [Q9Y6W5-2] |
PTM databases
GlyGeni | Q9Y6W5, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9Y6W5 |
PhosphoSitePlusi | Q9Y6W5 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000158195, Expressed in female reproductive system and 247 other tissues |
Genevisiblei | Q9Y6W5, HS |
Organism-specific databases
HPAi | ENSG00000158195, Low tissue specificity |
Interactioni
Subunit structurei
Binds actin and the Arp2/3 complex.
Interacts with BAIAP2.
Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 (NCKAP1l/HEM1 in hematopoietic cells) and WASF2/WAVE2 (PubMed:16417406). Directly interacts with BRK1.
Interacts with FNBP1L (via the SH3 domain) (PubMed:19798448).
7 Publications(Microbial infection) Interacts with human cytomegalovirus protein UL135.
1 PublicationBinary interactionsi
Q9Y6W5
GO - Molecular functioni
- actin binding Source: ProtInc
- cadherin binding Source: BHF-UCL
- protein kinase A binding Source: UniProtKB
- protein kinase A regulatory subunit binding Source: GO_Central
- SH3 domain binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 115465, 102 interactors |
CORUMi | Q9Y6W5 |
DIPi | DIP-41817N |
ELMi | Q9Y6W5 |
IntActi | Q9Y6W5, 36 interactors |
MINTi | Q9Y6W5 |
STRINGi | 9606.ENSP00000483313 |
Miscellaneous databases
RNActi | Q9Y6W5, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9Y6W5 |
SMRi | Q9Y6W5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9Y6W5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 436 – 453 | WH2PROSITE-ProRule annotationAdd BLAST | 18 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 173 – 203 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 240 – 435 | DisorderedSequence analysisAdd BLAST | 196 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 184 – 203 | Basic and acidic residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 240 – 262 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 295 – 413 | Pro residuesSequence analysisAdd BLAST | 119 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1830, Eukaryota |
GeneTreei | ENSGT00950000182962 |
HOGENOMi | CLU_036022_2_0_1 |
InParanoidi | Q9Y6W5 |
OMAi | WEKIKMG |
OrthoDBi | 1183697at2759 |
PhylomeDBi | Q9Y6W5 |
TreeFami | TF315031 |
Family and domain databases
InterProi | View protein in InterPro IPR028288, SCAR/WAVE_fam IPR003124, WH2_dom |
PANTHERi | PTHR12902, PTHR12902, 1 hit |
Pfami | View protein in Pfam PF02205, WH2, 1 hit |
SMARTi | View protein in SMART SM00246, WH2, 1 hit |
PROSITEi | View protein in PROSITE PS51082, WH2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPLVTRNIEP RHLCRQTLPS VRSELECVTN ITLANVIRQL GSLSKYAEDI
60 70 80 90 100
FGELFTQANT FASRVSSLAE RVDRLQVKVT QLDPKEEEVS LQGINTRKAF
110 120 130 140 150
RSSTIQDQKL FDRNSLPVPV LETYNTCDTP PPLNNLTPYR DDGKEALKFY
160 170 180 190 200
TDPSYFFDLW KEKMLQDTKD IMKEKRKHRK EKKDNPNRGN VNPRKIKTRK
210 220 230 240 250
EEWEKMKMGQ EFVESKEKLG TSGYPPTLVY QNGSIGCVEN VDASSYPPPP
260 270 280 290 300
QSDSASSPSP SFSEDNLPPP PAEFSYPVDN QRGSGLAGPK RSSVVSPSHP
310 320 330 340 350
PPAPPLGSPP GPKPGFAPPP APPPPPPPMI GIPPPPPPVG FGSPGTPPPP
360 370 380 390 400
SPPSFPPHPD FAAPPPPPPP PAADYPTLPP PPLSQPTGGA PPPPPPPPPP
410 420 430 440 450
GPPPPPFTGA DGQPAIPPPL SDTTKPKSSL PAVSDARSDL LSAIRQGFQL
460 470 480 490
RRVEEQREQE KRDVVGNDVA TILSRRIAVE YSDSEDDSSE FDEDDWSD
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042514 | 275 – 281 | SYPVDNQ → RFSAAQG in isoform 2. 1 Publication | 7 | |
Alternative sequenceiVSP_042515 | 282 – 498 | Missing in isoform 2. 1 PublicationAdd BLAST | 217 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB026542 mRNA Translation: BAA81795.1 AK303011 mRNA Translation: BAG64142.1 AL096774 Genomic DNA Translation: CAC18518.1 AL663122 Genomic DNA No translation available. BX293535 Genomic DNA No translation available. BC040943 mRNA Translation: AAH40943.1 |
CCDSi | CCDS304.1 [Q9Y6W5-1] CCDS55582.1 [Q9Y6W5-2] |
RefSeqi | NP_001188333.1, NM_001201404.2 [Q9Y6W5-2] NP_008921.1, NM_006990.4 [Q9Y6W5-1] |
Genome annotation databases
Ensembli | ENST00000536657.1; ENSP00000439883.1; ENSG00000158195.11 [Q9Y6W5-2] ENST00000618852.5; ENSP00000483313.1; ENSG00000158195.11 |
GeneIDi | 10163 |
KEGGi | hsa:10163 |
MANE-Selecti | ENST00000618852.5; ENSP00000483313.1; NM_006990.5; NP_008921.1 |
UCSCi | uc057dvq.1, human [Q9Y6W5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB026542 mRNA Translation: BAA81795.1 AK303011 mRNA Translation: BAG64142.1 AL096774 Genomic DNA Translation: CAC18518.1 AL663122 Genomic DNA No translation available. BX293535 Genomic DNA No translation available. BC040943 mRNA Translation: AAH40943.1 |
CCDSi | CCDS304.1 [Q9Y6W5-1] CCDS55582.1 [Q9Y6W5-2] |
RefSeqi | NP_001188333.1, NM_001201404.2 [Q9Y6W5-2] NP_008921.1, NM_006990.4 [Q9Y6W5-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2A40 | X-ray | 1.80 | C/F | 433-464 | [»] | |
AlphaFoldDBi | Q9Y6W5 | |||||
SMRi | Q9Y6W5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115465, 102 interactors |
CORUMi | Q9Y6W5 |
DIPi | DIP-41817N |
ELMi | Q9Y6W5 |
IntActi | Q9Y6W5, 36 interactors |
MINTi | Q9Y6W5 |
STRINGi | 9606.ENSP00000483313 |
PTM databases
GlyGeni | Q9Y6W5, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9Y6W5 |
PhosphoSitePlusi | Q9Y6W5 |
Genetic variation databases
BioMutai | WASF2 |
DMDMi | 59800456 |
Proteomic databases
EPDi | Q9Y6W5 |
jPOSTi | Q9Y6W5 |
MassIVEi | Q9Y6W5 |
MaxQBi | Q9Y6W5 |
PaxDbi | Q9Y6W5 |
PeptideAtlasi | Q9Y6W5 |
PRIDEi | Q9Y6W5 |
ProteomicsDBi | 86801 [Q9Y6W5-1] 86802 [Q9Y6W5-2] |
Protocols and materials databases
Antibodypediai | 30784, 353 antibodies from 32 providers |
DNASUi | 10163 |
Genome annotation databases
Ensembli | ENST00000536657.1; ENSP00000439883.1; ENSG00000158195.11 [Q9Y6W5-2] ENST00000618852.5; ENSP00000483313.1; ENSG00000158195.11 |
GeneIDi | 10163 |
KEGGi | hsa:10163 |
MANE-Selecti | ENST00000618852.5; ENSP00000483313.1; NM_006990.5; NP_008921.1 |
UCSCi | uc057dvq.1, human [Q9Y6W5-1] |
Organism-specific databases
CTDi | 10163 |
DisGeNETi | 10163 |
GeneCardsi | WASF2 |
HGNCi | HGNC:12733, WASF2 |
HPAi | ENSG00000158195, Low tissue specificity |
MIMi | 605875, gene |
neXtProti | NX_Q9Y6W5 |
OpenTargetsi | ENSG00000158195 |
PharmGKBi | PA37344 |
VEuPathDBi | HostDB:ENSG00000158195 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1830, Eukaryota |
GeneTreei | ENSGT00950000182962 |
HOGENOMi | CLU_036022_2_0_1 |
InParanoidi | Q9Y6W5 |
OMAi | WEKIKMG |
OrthoDBi | 1183697at2759 |
PhylomeDBi | Q9Y6W5 |
TreeFami | TF315031 |
Enzyme and pathway databases
PathwayCommonsi | Q9Y6W5 |
Reactomei | R-HSA-2029482, Regulation of actin dynamics for phagocytic cup formation R-HSA-4420097, VEGFA-VEGFR2 Pathway R-HSA-5663213, RHO GTPases Activate WASPs and WAVEs R-HSA-9013149, RAC1 GTPase cycle R-HSA-9013404, RAC2 GTPase cycle R-HSA-9013423, RAC3 GTPase cycle R-HSA-9664422, FCGR3A-mediated phagocytosis |
SignaLinki | Q9Y6W5 |
SIGNORi | Q9Y6W5 |
Miscellaneous databases
BioGRID-ORCSi | 10163, 88 hits in 1078 CRISPR screens |
ChiTaRSi | WASF2, human |
EvolutionaryTracei | Q9Y6W5 |
GeneWikii | WASF2 |
GenomeRNAii | 10163 |
Pharosi | Q9Y6W5, Tbio |
PROi | PR:Q9Y6W5 |
RNActi | Q9Y6W5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000158195, Expressed in female reproductive system and 247 other tissues |
Genevisiblei | Q9Y6W5, HS |
Family and domain databases
InterProi | View protein in InterPro IPR028288, SCAR/WAVE_fam IPR003124, WH2_dom |
PANTHERi | PTHR12902, PTHR12902, 1 hit |
Pfami | View protein in Pfam PF02205, WH2, 1 hit |
SMARTi | View protein in SMART SM00246, WH2, 1 hit |
PROSITEi | View protein in PROSITE PS51082, WH2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | WASF2_HUMAN | |
Accessioni | Q9Y6W5Primary (citable) accession number: Q9Y6W5 Secondary accession number(s): B4DZN0, O60794, Q9UDY7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | February 1, 2005 | |
Last modified: | May 25, 2022 | |
This is version 191 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families