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Protein

AP-1 complex subunit mu-2

Gene

AP1M2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-164940 Nef mediated downregulation of MHC class I complex cell surface expression
R-HSA-2132295 MHC class II antigen presentation
R-HSA-432720 Lysosome Vesicle Biogenesis
R-HSA-432722 Golgi Associated Vesicle Biogenesis
SignaLinkiQ9Y6Q5

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit mu-2
Alternative name(s):
AP-mu chain family member mu1B
Adaptor protein complex AP-1 subunit mu-2
Adaptor-related protein complex 1 subunit mu-2
Clathrin assembly protein complex 1 mu-2 medium chain 2
Golgi adaptor HA1/AP1 adaptin mu-2 subunit
Mu-adaptin 2
Mu1B-adaptin
Gene namesi
Name:AP1M2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000129354.11
HGNCiHGNC:558 AP1M2
MIMi607309 gene
neXtProtiNX_Q9Y6Q5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10053
OpenTargetsiENSG00000129354
PharmGKBiPA24849

Polymorphism and mutation databases

BioMutaiAP1M2
DMDMi13123953

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001937721 – 423AP-1 complex subunit mu-2Add BLAST423

Post-translational modificationi

Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y6Q5
PaxDbiQ9Y6Q5
PeptideAtlasiQ9Y6Q5
PRIDEiQ9Y6Q5
ProteomicsDBi86763
86764 [Q9Y6Q5-2]

PTM databases

iPTMnetiQ9Y6Q5
PhosphoSitePlusiQ9Y6Q5

Expressioni

Gene expression databases

BgeeiENSG00000129354 Expressed in 132 organ(s), highest expression level in mucosa of transverse colon
CleanExiHS_AP1M2
ExpressionAtlasiQ9Y6Q5 baseline and differential
GenevisibleiQ9Y6Q5 HS

Organism-specific databases

HPAiHPA054999

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).

Binary interactionsi

WithEntry#Exp.IntActNotes
AP2B1P630104EBI-752250,EBI-432924

Protein-protein interaction databases

BioGridi115364, 35 interactors
CORUMiQ9Y6Q5
IntActiQ9Y6Q5, 7 interactors
STRINGi9606.ENSP00000250244

Structurei

3D structure databases

ProteinModelPortaliQ9Y6Q5
SMRiQ9Y6Q5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini168 – 421MHDPROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0937 Eukaryota
ENOG410XPFS LUCA
GeneTreeiENSGT00530000062779
HOGENOMiHOG000173247
HOVERGENiHBG050516
InParanoidiQ9Y6Q5
KOiK12393
OMAiNAIVWSI
OrthoDBiEOG091G06XI
PhylomeDBiQ9Y6Q5
TreeFamiTF300393

Family and domain databases

InterProiView protein in InterPro
IPR036168 AP2_Mu_C_sf
IPR022775 AP_mu_sigma_su
IPR001392 Clathrin_mu
IPR018240 Clathrin_mu_CS
IPR011012 Longin-like_dom_sf
IPR028565 MHD
PfamiView protein in Pfam
PF00928 Adap_comp_sub, 1 hit
PF01217 Clat_adaptor_s, 1 hit
PIRSFiPIRSF005992 Clathrin_mu, 1 hit
PRINTSiPR00314 CLATHRINADPT
SUPFAMiSSF49447 SSF49447, 1 hit
SSF64356 SSF64356, 1 hit
PROSITEiView protein in PROSITE
PS00990 CLAT_ADAPTOR_M_1, 1 hit
PS00991 CLAT_ADAPTOR_M_2, 1 hit
PS51072 MHD, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Y6Q5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSASAVFILD VKGKPLISRN YKGDVAMSKI EHFMPLLVQR EEEGALAPLL
60 70 80 90 100
SHGQVHFLWI KHSNLYLVAT TSKNANASLV YSFLYKTIEV FCEYFKELEE
110 120 130 140 150
ESIRDNFVIV YELLDELMDF GFPQTTDSKI LQEYITQQSN KLETGKSRVP
160 170 180 190 200
PTVTNAVSWR SEGIKYKKNE VFIDVIESVN LLVNANGSVL LSEIVGTIKL
210 220 230 240 250
KVFLSGMPEL RLGLNDRVLF ELTGRSKNKS VELEDVKFHQ CVRLSRFDND
260 270 280 290 300
RTISFIPPDG DFELMSYRLS TQVKPLIWIE SVIEKFSHSR VEIMVKAKGQ
310 320 330 340 350
FKKQSVANGV EISVPVPSDA DSPRFKTSVG SAKYVPERNV VIWSIKSFPG
360 370 380 390 400
GKEYLMRAHF GLPSVEKEEV EGRPPIGVKF EIPYFTVSGI QVRYMKIIEK
410 420
SGYQALPWVR YITQSGDYQL RTS
Length:423
Mass (Da):48,108
Last modified:January 23, 2007 - v4
Checksum:iA3F6FDF8659DD6F7
GO
Isoform 2 (identifier: Q9Y6Q5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-225: R → LSG

Note: No experimental confirmation available.
Show »
Length:425
Mass (Da):48,210
Checksum:i93CA6C8D3A74ABBF
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7EPR4K7EPR4_HUMAN
AP-1 complex subunit mu-2
AP1M2
239Annotation score:
K7EMG5K7EMG5_HUMAN
AP-1 complex subunit mu-2
AP1M2
299Annotation score:
K7EJJ1K7EJJ1_HUMAN
AP-1 complex subunit mu-2
AP1M2
245Annotation score:
K7ERH5K7ERH5_HUMAN
AP-1 complex subunit mu-2
AP1M2
103Annotation score:
K7EL08K7EL08_HUMAN
AP-1 complex subunit mu-2
AP1M2
128Annotation score:
K7EK69K7EK69_HUMAN
AP-1 complex subunit mu-2
AP1M2
44Annotation score:
K7EPI9K7EPI9_HUMAN
AP-1 complex subunit mu-2
AP1M2
38Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti364S → R in AAH05021 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000169225R → LSG in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020797 mRNA Translation: AAD25870.2
AK315689 mRNA Translation: BAG38052.1
CH471106 Genomic DNA Translation: EAW84124.1
BC003387 mRNA Translation: AAH03387.1
BC003612 mRNA Translation: AAH03612.1
BC005021 mRNA Translation: AAH05021.1
CCDSiCCDS45964.1 [Q9Y6Q5-1]
CCDS77234.1 [Q9Y6Q5-2]
RefSeqiNP_001287816.1, NM_001300887.1 [Q9Y6Q5-2]
NP_005489.2, NM_005498.4 [Q9Y6Q5-1]
UniGeneiHs.18894

Genome annotation databases

EnsembliENST00000250244; ENSP00000250244; ENSG00000129354 [Q9Y6Q5-1]
ENST00000590923; ENSP00000465685; ENSG00000129354 [Q9Y6Q5-2]
GeneIDi10053
KEGGihsa:10053
UCSCiuc002mpc.4 human [Q9Y6Q5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020797 mRNA Translation: AAD25870.2
AK315689 mRNA Translation: BAG38052.1
CH471106 Genomic DNA Translation: EAW84124.1
BC003387 mRNA Translation: AAH03387.1
BC003612 mRNA Translation: AAH03612.1
BC005021 mRNA Translation: AAH05021.1
CCDSiCCDS45964.1 [Q9Y6Q5-1]
CCDS77234.1 [Q9Y6Q5-2]
RefSeqiNP_001287816.1, NM_001300887.1 [Q9Y6Q5-2]
NP_005489.2, NM_005498.4 [Q9Y6Q5-1]
UniGeneiHs.18894

3D structure databases

ProteinModelPortaliQ9Y6Q5
SMRiQ9Y6Q5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115364, 35 interactors
CORUMiQ9Y6Q5
IntActiQ9Y6Q5, 7 interactors
STRINGi9606.ENSP00000250244

PTM databases

iPTMnetiQ9Y6Q5
PhosphoSitePlusiQ9Y6Q5

Polymorphism and mutation databases

BioMutaiAP1M2
DMDMi13123953

Proteomic databases

EPDiQ9Y6Q5
PaxDbiQ9Y6Q5
PeptideAtlasiQ9Y6Q5
PRIDEiQ9Y6Q5
ProteomicsDBi86763
86764 [Q9Y6Q5-2]

Protocols and materials databases

DNASUi10053
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000250244; ENSP00000250244; ENSG00000129354 [Q9Y6Q5-1]
ENST00000590923; ENSP00000465685; ENSG00000129354 [Q9Y6Q5-2]
GeneIDi10053
KEGGihsa:10053
UCSCiuc002mpc.4 human [Q9Y6Q5-1]

Organism-specific databases

CTDi10053
DisGeNETi10053
EuPathDBiHostDB:ENSG00000129354.11
GeneCardsiAP1M2
HGNCiHGNC:558 AP1M2
HPAiHPA054999
MIMi607309 gene
neXtProtiNX_Q9Y6Q5
OpenTargetsiENSG00000129354
PharmGKBiPA24849
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0937 Eukaryota
ENOG410XPFS LUCA
GeneTreeiENSGT00530000062779
HOGENOMiHOG000173247
HOVERGENiHBG050516
InParanoidiQ9Y6Q5
KOiK12393
OMAiNAIVWSI
OrthoDBiEOG091G06XI
PhylomeDBiQ9Y6Q5
TreeFamiTF300393

Enzyme and pathway databases

ReactomeiR-HSA-164940 Nef mediated downregulation of MHC class I complex cell surface expression
R-HSA-2132295 MHC class II antigen presentation
R-HSA-432720 Lysosome Vesicle Biogenesis
R-HSA-432722 Golgi Associated Vesicle Biogenesis
SignaLinkiQ9Y6Q5

Miscellaneous databases

ChiTaRSiAP1M2 human
GeneWikiiAP1M2
GenomeRNAii10053
PROiPR:Q9Y6Q5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000129354 Expressed in 132 organ(s), highest expression level in mucosa of transverse colon
CleanExiHS_AP1M2
ExpressionAtlasiQ9Y6Q5 baseline and differential
GenevisibleiQ9Y6Q5 HS

Family and domain databases

InterProiView protein in InterPro
IPR036168 AP2_Mu_C_sf
IPR022775 AP_mu_sigma_su
IPR001392 Clathrin_mu
IPR018240 Clathrin_mu_CS
IPR011012 Longin-like_dom_sf
IPR028565 MHD
PfamiView protein in Pfam
PF00928 Adap_comp_sub, 1 hit
PF01217 Clat_adaptor_s, 1 hit
PIRSFiPIRSF005992 Clathrin_mu, 1 hit
PRINTSiPR00314 CLATHRINADPT
SUPFAMiSSF49447 SSF49447, 1 hit
SSF64356 SSF64356, 1 hit
PROSITEiView protein in PROSITE
PS00990 CLAT_ADAPTOR_M_1, 1 hit
PS00991 CLAT_ADAPTOR_M_2, 1 hit
PS51072 MHD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAP1M2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6Q5
Secondary accession number(s): B2RDV5, Q9BSI8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 156 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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