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Protein

Adenylate kinase isoenzyme 5

Gene

AK5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Also displays broad nucleoside diphosphate kinase activity.2 Publications

Catalytic activityi

ATP + AMP = 2 ADP.1 Publication
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei168AMP 1By similarity1
Binding sitei226AMP 1By similarity1
Binding sitei257ATP 1By similarity1
Binding sitei263AMP 1By similarity1
Binding sitei274AMP 1By similarity1
Binding sitei407AMP 2By similarity1
Binding sitei412AMP 2By similarity1
Binding sitei469AMP 2By similarity1
Binding sitei500ATP 2By similarity1
Binding sitei517AMP 2By similarity1
Binding sitei545ATP 2; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi142 – 147ATP 1By similarity6
Nucleotide bindingi191 – 193AMP 1By similarity3
Nucleotide bindingi219 – 222AMP 1By similarity4
Nucleotide bindingi386 – 391ATP 2By similarity6
Nucleotide bindingi433 – 435AMP 2By similarity3
Nucleotide bindingi462 – 465AMP 2By similarity4

GO - Molecular functioni

  • adenylate kinase activity Source: ProtInc
  • ATP binding Source: UniProtKB-KW
  • nucleoside diphosphate kinase activity Source: UniProtKB
  • nucleoside kinase activity Source: Reactome

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS07943-MONOMER
BRENDAi2.7.4.3 2681
ReactomeiR-HSA-499943 Interconversion of nucleotide di- and triphosphates

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase isoenzyme 5 (EC:2.7.4.3, EC:2.7.4.6)
Short name:
AK 5
Alternative name(s):
ATP-AMP transphosphorylase 5
Gene namesi
Name:AK5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000154027.18
HGNCiHGNC:365 AK5
MIMi608009 gene
neXtProtiNX_Q9Y6K8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi26289
OpenTargetsiENSG00000154027
PharmGKBiPA24659

Polymorphism and mutation databases

BioMutaiAK5
DMDMi257051028

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001589301 – 562Adenylate kinase isoenzyme 5Add BLAST562

Proteomic databases

EPDiQ9Y6K8
PaxDbiQ9Y6K8
PeptideAtlasiQ9Y6K8
PRIDEiQ9Y6K8
ProteomicsDBi86713
86714 [Q9Y6K8-2]
86715 [Q9Y6K8-3]

PTM databases

iPTMnetiQ9Y6K8
PhosphoSitePlusiQ9Y6K8

Expressioni

Tissue specificityi

Brain specific.1 Publication

Gene expression databases

BgeeiENSG00000154027 Expressed in 143 organ(s), highest expression level in corpus callosum
CleanExiHS_AK5
ExpressionAtlasiQ9Y6K8 baseline and differential
GenevisibleiQ9Y6K8 HS

Organism-specific databases

HPAiHPA019128
HPA057255

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi117670, 8 interactors
IntActiQ9Y6K8, 9 interactors
STRINGi9606.ENSP00000346577

Structurei

Secondary structure

1562
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y6K8
SMRiQ9Y6K8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y6K8

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 316Adenylate kinase 11 PublicationAdd BLAST184
Regioni162 – 193NMPbind 1By similarityAdd BLAST32
Regioni256 – 266LID 1By similarityAdd BLAST11
Regioni377 – 559Adenylate kinase 21 PublicationAdd BLAST183
Regioni406 – 435NMPbind 21 PublicationAdd BLAST30
Regioni499 – 509LID 21 PublicationAdd BLAST11

Sequence similaritiesi

Belongs to the adenylate kinase family.Curated

Phylogenomic databases

eggNOGiKOG3079 Eukaryota
COG0563 LUCA
GeneTreeiENSGT00390000016215
HOVERGENiHBG059001
InParanoidiQ9Y6K8
KOiK00939
OMAiCSNQRLK
OrthoDBiEOG091G0OQ7
PhylomeDBiQ9Y6K8
TreeFamiTF313747

Family and domain databases

CDDicd01428 ADK, 2 hits
HAMAPiMF_00235 Adenylate_kinase_Adk, 2 hits
InterProiView protein in InterPro
IPR000850 Adenylat/UMP-CMP_kin
IPR033690 Adenylat_kinase_CS
IPR006267 AK1/5
IPR027417 P-loop_NTPase
PANTHERiPTHR23359 PTHR23359, 1 hit
PRINTSiPR00094 ADENYLTKNASE
SUPFAMiSSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR01360 aden_kin_iso1, 1 hit
PROSITEiView protein in PROSITE
PS00113 ADENYLATE_KINASE, 2 hits

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Y6K8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNTNDAKEYL ARREIPQLFE SLLNGLMCSK PEDPVEYLES CLQKVKELGG
60 70 80 90 100
CDKVKWDTFV SQEKKTLPPL NGGQSRRSFL RNVMPENSNF PYRRYDRLPP
110 120 130 140 150
IHQFSIESDT DLSETAELIE EYEVFDPTRP RPKIILVIGG PGSGKGTQSL
160 170 180 190 200
KIAERYGFQY ISVGELLRKK IHSTSSNRKW SLIAKIITTG ELAPQETTIT
210 220 230 240 250
EIKQKLMQIP DEEGIVIDGF PRDVAQALSF EDQICTPDLV VFLACANQRL
260 270 280 290 300
KERLLKRAEQ QGRPDDNVKA TQRRLMNFKQ NAAPLVKYFQ EKGLIMTFDA
310 320 330 340 350
DRDEDEVFYD ISMAVDNKLF PNKEAAAGSS DLDPSMILDT GEIIDTGSDY
360 370 380 390 400
EDQGDDQLNV FGEDTMGGFM EDLRKCKIIF IIGGPGSGKG TQCEKLVEKY
410 420 430 440 450
GFTHLSTGEL LREELASESE RSKLIRDIME RGDLVPSGIV LELLKEAMVA
460 470 480 490 500
SLGDTRGFLI DGYPREVKQG EEFGRRIGDP QLVICMDCSA DTMTNRLLQR
510 520 530 540 550
SRSSLPVDDT TKTIAKRLEA YYRASIPVIA YYETKTQLHK INAEGTPEDV
560
FLQLCTAIDS IF
Length:562
Mass (Da):63,333
Last modified:September 1, 2009 - v2
Checksum:iCF7DB084924A782F
GO
Isoform 2 (identifier: Q9Y6K8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-365: Missing.

Note: It is unsure whether Met-1 or Met-5 is the initiator.
Show »
Length:197
Mass (Da):21,974
Checksum:iB63514CB7C3B58AF
GO
Isoform 3 (identifier: Q9Y6K8-3) [UniParc]FASTAAdd to basket
Also known as: Adenylate kinase 6

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Show »
Length:536
Mass (Da):60,315
Checksum:iD263FD0BABB4B82A
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PQQ8E9PQQ8_HUMAN
Adenylate kinase isoenzyme 5
AK5
176Annotation score:
E9PIS7E9PIS7_HUMAN
Adenylate kinase isoenzyme 5
AK5
77Annotation score:
H0YEZ1H0YEZ1_HUMAN
Adenylate kinase isoenzyme 5
AK5
62Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10L → M in AAH36666 (PubMed:15489334).Curated1
Sequence conflicti86E → G in AAH36666 (PubMed:15489334).Curated1
Sequence conflicti182L → P in AAO16520 (Ref. 2) Curated1
Sequence conflicti182L → P in AAH33896 (PubMed:15489334).Curated1
Sequence conflicti321P → S in AAO16520 (Ref. 2) Curated1
Sequence conflicti343I → T in AAO16520 (Ref. 2) Curated1
Sequence conflicti348S → Y in AAH33896 (PubMed:15489334).Curated1
Sequence conflicti385P → T in AAH33896 (PubMed:15489334).Curated1
Sequence conflicti561Missing in AAP97322 (Ref. 2) Curated1
Sequence conflicti561Missing in AAH33896 (PubMed:15489334).Curated1
Sequence conflicti561Missing in AAH12467 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_059435465R → Q. Corresponds to variant dbSNP:rs2803140Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0378781 – 365Missing in isoform 2. 3 PublicationsAdd BLAST365
Alternative sequenceiVSP_0378791 – 26Missing in isoform 3. 2 PublicationsAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062595 mRNA Translation: AAD27956.1
AY171600 mRNA Translation: AAO16520.2
AF445193 mRNA Translation: AAP97322.1
CR541890 mRNA Translation: CAG46688.1
BC033896 mRNA Translation: AAH33896.1
BC036666 mRNA Translation: AAH36666.1
BC012467 mRNA Translation: AAH12467.2
CCDSiCCDS675.1 [Q9Y6K8-1]
CCDS676.1 [Q9Y6K8-3]
RefSeqiNP_036225.2, NM_012093.3 [Q9Y6K8-3]
NP_777283.1, NM_174858.2 [Q9Y6K8-1]
XP_006710635.1, XM_006710572.3 [Q9Y6K8-3]
XP_016856497.1, XM_017001008.1 [Q9Y6K8-3]
XP_016856498.1, XM_017001009.1 [Q9Y6K8-3]
UniGeneiHs.559718
Hs.597002

Genome annotation databases

EnsembliENST00000344720; ENSP00000341430; ENSG00000154027 [Q9Y6K8-3]
ENST00000354567; ENSP00000346577; ENSG00000154027 [Q9Y6K8-1]
GeneIDi26289
KEGGihsa:26289
UCSCiuc001dhn.4 human [Q9Y6K8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062595 mRNA Translation: AAD27956.1
AY171600 mRNA Translation: AAO16520.2
AF445193 mRNA Translation: AAP97322.1
CR541890 mRNA Translation: CAG46688.1
BC033896 mRNA Translation: AAH33896.1
BC036666 mRNA Translation: AAH36666.1
BC012467 mRNA Translation: AAH12467.2
CCDSiCCDS675.1 [Q9Y6K8-1]
CCDS676.1 [Q9Y6K8-3]
RefSeqiNP_036225.2, NM_012093.3 [Q9Y6K8-3]
NP_777283.1, NM_174858.2 [Q9Y6K8-1]
XP_006710635.1, XM_006710572.3 [Q9Y6K8-3]
XP_016856497.1, XM_017001008.1 [Q9Y6K8-3]
XP_016856498.1, XM_017001009.1 [Q9Y6K8-3]
UniGeneiHs.559718
Hs.597002

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BWJX-ray2.30A/B/C/D/E/F366-562[»]
ProteinModelPortaliQ9Y6K8
SMRiQ9Y6K8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117670, 8 interactors
IntActiQ9Y6K8, 9 interactors
STRINGi9606.ENSP00000346577

PTM databases

iPTMnetiQ9Y6K8
PhosphoSitePlusiQ9Y6K8

Polymorphism and mutation databases

BioMutaiAK5
DMDMi257051028

Proteomic databases

EPDiQ9Y6K8
PaxDbiQ9Y6K8
PeptideAtlasiQ9Y6K8
PRIDEiQ9Y6K8
ProteomicsDBi86713
86714 [Q9Y6K8-2]
86715 [Q9Y6K8-3]

Protocols and materials databases

DNASUi26289
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000344720; ENSP00000341430; ENSG00000154027 [Q9Y6K8-3]
ENST00000354567; ENSP00000346577; ENSG00000154027 [Q9Y6K8-1]
GeneIDi26289
KEGGihsa:26289
UCSCiuc001dhn.4 human [Q9Y6K8-1]

Organism-specific databases

CTDi26289
DisGeNETi26289
EuPathDBiHostDB:ENSG00000154027.18
GeneCardsiAK5
H-InvDBiHIX0020812
HGNCiHGNC:365 AK5
HPAiHPA019128
HPA057255
MIMi608009 gene
neXtProtiNX_Q9Y6K8
OpenTargetsiENSG00000154027
PharmGKBiPA24659
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3079 Eukaryota
COG0563 LUCA
GeneTreeiENSGT00390000016215
HOVERGENiHBG059001
InParanoidiQ9Y6K8
KOiK00939
OMAiCSNQRLK
OrthoDBiEOG091G0OQ7
PhylomeDBiQ9Y6K8
TreeFamiTF313747

Enzyme and pathway databases

BioCyciMetaCyc:HS07943-MONOMER
BRENDAi2.7.4.3 2681
ReactomeiR-HSA-499943 Interconversion of nucleotide di- and triphosphates

Miscellaneous databases

ChiTaRSiAK5 human
EvolutionaryTraceiQ9Y6K8
GenomeRNAii26289
PROiPR:Q9Y6K8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000154027 Expressed in 143 organ(s), highest expression level in corpus callosum
CleanExiHS_AK5
ExpressionAtlasiQ9Y6K8 baseline and differential
GenevisibleiQ9Y6K8 HS

Family and domain databases

CDDicd01428 ADK, 2 hits
HAMAPiMF_00235 Adenylate_kinase_Adk, 2 hits
InterProiView protein in InterPro
IPR000850 Adenylat/UMP-CMP_kin
IPR033690 Adenylat_kinase_CS
IPR006267 AK1/5
IPR027417 P-loop_NTPase
PANTHERiPTHR23359 PTHR23359, 1 hit
PRINTSiPR00094 ADENYLTKNASE
SUPFAMiSSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR01360 aden_kin_iso1, 1 hit
PROSITEiView protein in PROSITE
PS00113 ADENYLATE_KINASE, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiKAD5_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6K8
Secondary accession number(s): Q5U622
, Q6FH66, Q7Z4T5, Q86YS0, Q8N464, Q96EC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: September 1, 2009
Last modified: November 7, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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