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Protein

2'-5'-oligoadenylate synthase 3

Gene

OAS3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes preferentially dimers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. Displays antiviral activity against Chikungunya virus (CHIKV), Dengue virus, Sindbis virus (SINV) and Semliki forest virus (SFV).3 Publications

Catalytic activityi

3 ATP = pppA2'p5'A2'p5'A + 2 diphosphate.2 Publications

Cofactori

Mg2+1 Publication

Activity regulationi

Produced as a latent enzyme which is activated by dsRNA generated during the course of viral infection (Probable). Strongly activated by long dsRNAs at least 50 nucleotides in length (PubMed:25775560). ssRNA does not activate the enzyme (PubMed:25775560).Curated1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei804ATPBy similarity1
Metal bindingi816Magnesium; catalytic1 Publication1
Metal bindingi818Magnesium; catalytic1 Publication1
Metal bindingi888Magnesium; catalyticBy similarity1
Binding sitei947ATPBy similarity1
Binding sitei950ATPBy similarity1
Binding sitei969ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
Biological processAntiviral defense, Immunity, Innate immunity
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000111331-MONOMER
ReactomeiR-HSA-877300 Interferon gamma signaling
R-HSA-909733 Interferon alpha/beta signaling

Names & Taxonomyi

Protein namesi
Recommended name:
2'-5'-oligoadenylate synthase 3 (EC:2.7.7.842 Publications)
Short name:
(2-5')oligo(A) synthase 3
Short name:
2-5A synthase 3
Alternative name(s):
p100 OAS
Short name:
p100OAS
Gene namesi
Name:OAS3
ORF Names:P/OKcl.4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000111331.12
HGNCiHGNC:8088 OAS3
MIMi603351 gene
neXtProtiNX_Q9Y6K5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30R → A: Impaired dsRNA binding. 1 Publication1
Mutagenesisi41R → A: Impaired dsRNA binding. 1 Publication1
Mutagenesisi76E → D: No effect on catalytic activity; when associated with D-145. 1 Publication1
Mutagenesisi145S → D: No effect on catalytic activity. No effect on catalytic activity; when associated with D-76. 1 Publication1
Mutagenesisi816 – 818DAD → AAA: Abolishes catalytic activity. 1 Publication3

Organism-specific databases

DisGeNETi4940
OpenTargetsiENSG00000111331
PharmGKBiPA31877

Polymorphism and mutation databases

BioMutaiOAS3
DMDMi317373408

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001602651 – 10872'-5'-oligoadenylate synthase 3Add BLAST1087

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei365PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Y6K5
MaxQBiQ9Y6K5
PaxDbiQ9Y6K5
PeptideAtlasiQ9Y6K5
PRIDEiQ9Y6K5
ProteomicsDBi86712

PTM databases

iPTMnetiQ9Y6K5
PhosphoSitePlusiQ9Y6K5

Expressioni

Tissue specificityi

Present at high level in placenta trophoblast.

Inductioni

By type I interferon (IFN) and viruses.

Gene expression databases

BgeeiENSG00000111331 Expressed in 189 organ(s), highest expression level in epithelium of bronchus
CleanExiHS_OAS3
ExpressionAtlasiQ9Y6K5 baseline and differential
GenevisibleiQ9Y6K5 HS

Organism-specific databases

HPAiHPA041253
HPA041372

Interactioni

Subunit structurei

Monomer.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei155Interaction with dsRNA1 Publication1
Sitei244Interaction with dsRNA1 Publication1

Binary interactionsi

Protein-protein interaction databases

BioGridi110994, 17 interactors
IntActiQ9Y6K5, 15 interactors
STRINGi9606.ENSP00000228928

Structurei

Secondary structure

11087
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y6K5
SMRiQ9Y6K5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni6 – 343OAS domain 1CuratedAdd BLAST338
Regioni12 – 57Interaction with dsRNA1 PublicationAdd BLAST46
Regioni186 – 200Interaction with dsRNA1 PublicationAdd BLAST15
Regioni344 – 410LinkerCuratedAdd BLAST67
Regioni411 – 742OAS domain 2CuratedAdd BLAST332
Regioni750 – 1084OAS domain 3CuratedAdd BLAST335

Domaini

OAS domain 3 is catalytically active. OAS domain 1 has no catalytic activity but is essential for recognition of long dsRNAs.1 Publication

Sequence similaritiesi

Belongs to the 2-5A synthase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0001 Eukaryota
COG5272 LUCA
GeneTreeiENSGT00510000046406
HOGENOMiHOG000013200
HOVERGENiHBG007856
KOiK14216
OMAiFNMAEGF
OrthoDBiEOG091G0160
PhylomeDBiQ9Y6K5
TreeFamiTF329749

Family and domain databases

Gene3Di1.10.1410.20, 3 hits
InterProiView protein in InterPro
IPR006117 2-5-oligoadenylate_synth_CS
IPR006116 2-5-oligoadenylate_synth_N
IPR018952 2-5-oligoAdlate_synth_1_dom2/C
IPR038121 2-5-oligoAdlate_synth_2_sf
IPR026774 2-5A_synthase
IPR002934 Polymerase_NTP_transf_dom
PANTHERiPTHR11258 PTHR11258, 4 hits
PfamiView protein in Pfam
PF01909 NTP_transf_2, 1 hit
PF10421 OAS1_C, 3 hits
PROSITEiView protein in PROSITE
PS00832 25A_SYNTH_1, 3 hits
PS00833 25A_SYNTH_2, 2 hits
PS50152 25A_SYNTH_3, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.iShow all

Q9Y6K5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDLYSTPAAA LDRFVARRLQ PRKEFVEKAR RALGALAAAL RERGGRLGAA
60 70 80 90 100
APRVLKTVKG GSSGRGTALK GGCDSELVIF LDCFKSYVDQ RARRAEILSE
110 120 130 140 150
MRASLESWWQ NPVPGLRLTF PEQSVPGALQ FRLTSVDLED WMDVSLVPAF
160 170 180 190 200
NVLGQAGSGV KPKPQVYSTL LNSGCQGGEH AACFTELRRN FVNIRPAKLK
210 220 230 240 250
NLILLVKHWY HQVCLQGLWK ETLPPVYALE LLTIFAWEQG CKKDAFSLAE
260 270 280 290 300
GLRTVLGLIQ QHQHLCVFWT VNYGFEDPAV GQFLQRQLKR PRPVILDPAD
310 320 330 340 350
PTWDLGNGAA WHWDLLAQEA ASCYDHPCFL RGMGDPVQSW KGPGLPRAGC
360 370 380 390 400
SGLGHPIQLD PNQKTPENSK SLNAVYPRAG SKPPSCPAPG PTGAASIVPS
410 420 430 440 450
VPGMALDLSQ IPTKELDRFI QDHLKPSPQF QEQVKKAIDI ILRCLHENCV
460 470 480 490 500
HKASRVSKGG SFGRGTDLRD GCDVELIIFL NCFTDYKDQG PRRAEILDEM
510 520 530 540 550
RAQLESWWQD QVPSLSLQFP EQNVPEALQF QLVSTALKSW TDVSLLPAFD
560 570 580 590 600
AVGQLSSGTK PNPQVYSRLL TSGCQEGEHK ACFAELRRNF MNIRPVKLKN
610 620 630 640 650
LILLVKHWYR QVAAQNKGKG PAPASLPPAY ALELLTIFAW EQGCRQDCFN
660 670 680 690 700
MAQGFRTVLG LVQQHQQLCV YWTVNYSTED PAMRMHLLGQ LRKPRPLVLD
710 720 730 740 750
PADPTWNVGH GSWELLAQEA AALGMQACFL SRDGTSVQPW DVMPALLYQT
760 770 780 790 800
PAGDLDKFIS EFLQPNRQFL AQVNKAVDTI CSFLKENCFR NSPIKVIKVV
810 820 830 840 850
KGGSSAKGTA LRGRSDADLV VFLSCFSQFT EQGNKRAEII SEIRAQLEAC
860 870 880 890 900
QQERQFEVKF EVSKWENPRV LSFSLTSQTM LDQSVDFDVL PAFDALGQLV
910 920 930 940 950
SGSRPSSQVY VDLIHSYSNA GEYSTCFTEL QRDFIISRPT KLKSLIRLVK
960 970 980 990 1000
HWYQQCTKIS KGRGSLPPQH GLELLTVYAW EQGGKDSQFN MAEGFRTVLE
1010 1020 1030 1040 1050
LVTQYRQLCI YWTINYNAKD KTVGDFLKQQ LQKPRPIILD PADPTGNLGH
1060 1070 1080
NARWDLLAKE AAACTSALCC MGRNGIPIQP WPVKAAV
Length:1,087
Mass (Da):121,170
Last modified:January 11, 2011 - v3
Checksum:i36DAADE4574DE961
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VS35F8VS35_HUMAN
2'-5'-oligoadenylate synthase 3
OAS3
223Annotation score:
F8VWK9F8VWK9_HUMAN
2'-5'-oligoadenylate synthase 3
OAS3
177Annotation score:
H0YHL3H0YHL3_HUMAN
2'-5'-oligoadenylate synthase 3
OAS3
166Annotation score:

Sequence cautioni

The sequence AAD28543 differs from that shown. Reason: Frameshift at positions 394 and 398.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti159G → A in AAD28543 (PubMed:9880533).Curated1
Sequence conflicti249A → G in AAD28543 (PubMed:9880533).Curated1
Sequence conflicti287 – 288QL → HV in AAD28543 (PubMed:9880533).Curated2
Sequence conflicti316L → H in AAD28543 (PubMed:9880533).Curated1
Sequence conflicti393G → A in AAD28543 (PubMed:9880533).Curated1
Sequence conflicti503 – 504QL → HV in AAD28543 (PubMed:9880533).Curated2
Sequence conflicti503 – 504QL → HV in BAB18647 (PubMed:11280764).Curated2
Sequence conflicti984G → R in BAB18647 (PubMed:11280764).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06007618R → K1 PublicationCorresponds to variant dbSNP:rs1859330Ensembl.1
Natural variantiVAR_06007718R → M. Corresponds to variant dbSNP:rs1859330Ensembl.1
Natural variantiVAR_06007818R → T. Corresponds to variant dbSNP:rs1859330Ensembl.1
Natural variantiVAR_05766065R → W. Corresponds to variant dbSNP:rs12819767Ensembl.1
Natural variantiVAR_062127378R → K. Corresponds to variant dbSNP:rs45519442Ensembl.1
Natural variantiVAR_057661381S → R1 PublicationCorresponds to variant dbSNP:rs2285933Ensembl.1
Natural variantiVAR_057662869R → H. Corresponds to variant dbSNP:rs16942374Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063613 mRNA Translation: AAD28543.1 Frameshift.
AB044545 mRNA Translation: BAB18647.1
AC004551 Genomic DNA No translation available.
BC113746 mRNA Translation: AAI13747.1
AF251351 Genomic DNA No translation available.
CCDSiCCDS44981.1
RefSeqiNP_006178.2, NM_006187.3
UniGeneiHs.528634
Hs.744211

Genome annotation databases

EnsembliENST00000228928; ENSP00000228928; ENSG00000111331
GeneIDi4940
KEGGihsa:4940
UCSCiuc001tug.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiOAS3_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6K5
Secondary accession number(s): Q2HJ14, Q9H3P5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 11, 2011
Last modified: September 12, 2018
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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