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Protein

Choline/ethanolaminephosphotransferase 1

Gene

CEPT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis from CDP-choline and CDP-ethanolamine, respectively. Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity.2 Publications

Catalytic activityi

CDP-ethanolamine + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylethanolamine.1 Publication
CDP-choline + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylcholine.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 Publication

Kineticsi

  1. KM=37 µM for CDP-choline2 Publications
  2. KM=101 µM for CDP-ethanolamine2 Publications
  1. Vmax=10.5 nmol/min/mg enzyme with CDP-choline as substrate2 Publications
  2. Vmax=4.35 nmol/min/mg enzyme with CDP-ethanolamine as substrate2 Publications

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Choline/ethanolamine kinase (CHKB), Ethanolamine kinase 2 (ETNK2), Choline kinase alpha (CHKA), Ethanolamine kinase 1 (ETNK1)
  2. Ethanolamine-phosphate cytidylyltransferase (PCYT2)
  3. Ethanolaminephosphotransferase 1 (SELENOI), Choline/ethanolaminephosphotransferase 1 (CEPT1)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Choline-phosphate cytidylyltransferase A (PCYT1A), Choline-phosphate cytidylyltransferase B (PCYT1B)
  2. Cholinephosphotransferase 1 (CHPT1), Choline/ethanolaminephosphotransferase 1 (CEPT1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS05844-MONOMER
ReactomeiR-HSA-1483191 Synthesis of PC
R-HSA-1483213 Synthesis of PE
SABIO-RKiQ9Y6K0
UniPathwayiUPA00558; UER00743
UPA00753; UER00740

Protein family/group databases

TCDBi4.F.1.1.1 the choline/ethanolaminephosphotransferase 1 (cept1) family

Chemistry databases

SwissLipidsiSLP:000000137

Names & Taxonomyi

Protein namesi
Recommended name:
Choline/ethanolaminephosphotransferase 1 (EC:2.7.8.1, EC:2.7.8.2)
Short name:
hCEPT1
Gene namesi
Name:CEPT1
ORF Names:PRO1101
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000134255.13
HGNCiHGNC:24289 CEPT1
MIMi616751 gene
neXtProtiNX_Q9Y6K0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Transmembranei115 – 135HelicalSequence analysisAdd BLAST21
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
Transmembranei209 – 229HelicalSequence analysisAdd BLAST21
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Transmembranei283 – 303HelicalSequence analysisAdd BLAST21
Transmembranei317 – 337HelicalSequence analysisAdd BLAST21
Transmembranei365 – 385HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi138K → M: Induces a reduction in both cholinephosphotransferase and ethanolaminephosphotransferase activities. 1
Mutagenesisi144N → G: No effect. 1 Publication1
Mutagenesisi146S → Q or C: No effect. 1 Publication1
Mutagenesisi156G → C, S or A: Induces a reduction in cholinephosphotransferase activity and abolishes ethanolaminephosphotransferase activity. 1 Publication1
Mutagenesisi214T → A: Alters the profile of diacylglycerol utilization and results in modest reduction in enzyme activity. 1 Publication1
Mutagenesisi215E → A or D: Induces a strong reduction in enzyme activity without altering diacylglycerol specificity. 1 Publication1
Mutagenesisi215E → Q: Induces a strong reduction in enzyme activity and alters diacylglycerol specificity. 1 Publication1
Mutagenesisi216V → A: Alters the profile of diacylglycerol utilization and results in modest reduction in enzyme activity. 1 Publication1
Mutagenesisi221I → A: Alters the profile of diacylglycerol utilization and results in modest reduction in enzyme activity. 1 Publication1
Mutagenesisi226L → A: Does not affect either the enzyme activity or the diacylglycerol specificity. 1 Publication1
Mutagenesisi228V → A: Does not affect either the enzyme activity or the diacylglycerol specificity. 1 Publication1

Organism-specific databases

DisGeNETi10390
OpenTargetsiENSG00000134255
PharmGKBiPA134892657

Chemistry databases

DrugBankiDB00122 Choline

Polymorphism and mutation databases

BioMutaiCEPT1
DMDMi74753524

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002892451 – 416Choline/ethanolaminephosphotransferase 1Add BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineCombined sources1
Modified residuei40PhosphothreonineCombined sources1
Glycosylationi144N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9Y6K0
MaxQBiQ9Y6K0
PaxDbiQ9Y6K0
PeptideAtlasiQ9Y6K0
PRIDEiQ9Y6K0
ProteomicsDBi86708

PTM databases

iPTMnetiQ9Y6K0
PhosphoSitePlusiQ9Y6K0
SwissPalmiQ9Y6K0

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000134255
CleanExiHS_CEPT1
ExpressionAtlasiQ9Y6K0 baseline and differential
GenevisibleiQ9Y6K0 HS

Interactioni

Protein-protein interaction databases

BioGridi115662, 31 interactors
IntActiQ9Y6K0, 32 interactors
MINTiQ9Y6K0
STRINGi9606.ENSP00000349696

Structurei

3D structure databases

ProteinModelPortaliQ9Y6K0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2877 Eukaryota
COG5050 LUCA
GeneTreeiENSGT00530000063048
HOGENOMiHOG000034808
HOVERGENiHBG107647
InParanoidiQ9Y6K0
KOiK13644
OMAiWGLQFAH
OrthoDBiEOG091G07XY
PhylomeDBiQ9Y6K0
TreeFamiTF313270

Family and domain databases

InterProiView protein in InterPro
IPR000462 CDP-OH_P_trans
IPR014472 CHOPT
PANTHERiPTHR10414 PTHR10414, 1 hit
PfamiView protein in Pfam
PF01066 CDP-OH_P_transf, 1 hit
PIRSFiPIRSF015665 CHOPT, 1 hit
PROSITEiView protein in PROSITE
PS00379 CDP_ALCOHOL_P_TRANSF, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Y6K0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGHRSTRKR CGDSHPESPV GFGHMSTTGC VLNKLFQLPT PPLSRHQLKR
60 70 80 90 100
LEEHRYQSAG RSLLEPLMQG YWEWLVRRVP SWIAPNLITI IGLSINICTT
110 120 130 140 150
ILLVFYCPTA TEQAPLWAYI ACACGLFIYQ SLDAIDGKQA RRTNSSSPLG
160 170 180 190 200
ELFDHGCDSL STVFVVLGTC IAVQLGTNPD WMFFCCFAGT FMFYCAHWQT
210 220 230 240 250
YVSGTLRFGI IDVTEVQIFI IIMHLLAVIG GPPFWQSMIP VLNIQMKIFP
260 270 280 290 300
ALCTVAGTIF SCTNYFRVIF TGGVGKNGST IAGTSVLSPF LHIGSVITLA
310 320 330 340 350
AMIYKKSAVQ LFEKHPCLYI LTFGFVSAKI TNKLVVAHMT KSEMHLHDTA
360 370 380 390 400
FIGPALLFLD QYFNSFIDEY IVLWIALVFS FFDLIRYCVS VCNQIASHLH
410
IHVFRIKVST AHSNHH
Length:416
Mass (Da):46,554
Last modified:November 1, 1999 - v1
Checksum:iA25FED1193342FD9
GO

Sequence cautioni

The sequence AAF61194 differs from that shown. Reason: Frameshift at position 276.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068302 mRNA Translation: AAD25170.1
AF138862 mRNA Translation: AAF61194.1 Frameshift.
AL355816 Genomic DNA No translation available.
BC032610 mRNA Translation: AAH32610.1
BC049196 mRNA Translation: AAH49196.1
AL833102 mRNA Translation: CAH10403.1
CCDSiCCDS830.1
RefSeqiNP_001007795.1, NM_001007794.2
NP_001317672.1, NM_001330743.1
NP_006081.1, NM_006090.4
UniGeneiHs.363572
Hs.636850

Genome annotation databases

EnsembliENST00000357172; ENSP00000349696; ENSG00000134255
ENST00000545121; ENSP00000441980; ENSG00000134255
GeneIDi10390
KEGGihsa:10390
UCSCiuc001eah.1 human

Similar proteinsi

Entry informationi

Entry nameiCEPT1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6K0
Secondary accession number(s): Q69YJ9, Q9P0Y8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 1, 1999
Last modified: July 18, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

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