UniProtKB - Q9Y6K0 (CEPT1_HUMAN)
Protein
Choline/ethanolaminephosphotransferase 1
Gene
CEPT1
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis from CDP-choline and CDP-ethanolamine, respectively. Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity.3 Publications
Catalytic activityi
- a 1,2-diacyl-sn-glycerol + CDP-ethanolamine = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CMP + H+1 PublicationEC:2.7.8.11 PublicationThis reaction proceeds in the forward1 Publication direction.
- a 1,2-diacyl-sn-glycerol + CDP-choline = a 1,2-diacyl-sn-glycero-3-phosphocholine + CMP + H+1 PublicationEC:2.7.8.21 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-dioctanoyl-sn-glycerol + CDP-choline = 1,2-dioctanoyl-sn-glycero-3-phosphocholine + CMP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-didecanoyl-sn-glycerol + CDP-choline = 1,2-didecanoyl-sn-glycero-3-phosphocholine + CMP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-di-(9Z-octadecenoyl)-sn-glycerol + CDP-choline = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + CMP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CDP-choline = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + CMP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-di-(9Z-octadecenoyl)-sn-glycerol + CDP-ethanolamine = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + CMP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CDP-ethanolamine = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + CMP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycerol + CDP-choline = 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + CMP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-di-(9Z-hexadecenoyl)-sn-glycerol + CDP-choline = 1,2-di-(9Z-hexadecenoyl)-sn-glycero-3-phosphocholine + CMP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-di-(9Z-hexadecenoyl)-sn-glycerol + CDP-ethanolamine = 1,2-di-(9Z-hexadecenoyl)-sn-glycero-3-phosphoethanolamine + CMP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-O-hexadecyl-2-acetyl-sn-glycerol + CDP-choline = 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + CMP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + CDP-choline = 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine + CMP + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Kineticsi
- KM=37 µM for CDP-choline2 Publications
- KM=101 µM for CDP-ethanolamine2 Publications
- Vmax=10.5 nmol/min/mg enzyme with CDP-choline as substrate2 Publications
- Vmax=4.35 nmol/min/mg enzyme with CDP-ethanolamine as substrate2 Publications
: phosphatidylethanolamine biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.2 PublicationsProteins known to be involved in the 3 steps of the subpathway in this organism are:
- Choline/ethanolamine kinase (CHKB), Ethanolamine kinase 2 (ETNK2), Ethanolamine kinase 1 (ETNK1), Choline kinase alpha (CHKA)
- Ethanolamine-phosphate cytidylyltransferase (PCYT2)
- Ethanolaminephosphotransferase 1 (SELENOI), Choline/ethanolaminephosphotransferase 1 (CEPT1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.
Pathwayi: phosphatidylcholine biosynthesis
This protein is involved in step 2 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.2 PublicationsProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Choline-phosphate cytidylyltransferase B (PCYT1B), Choline-phosphate cytidylyltransferase A (PCYT1A)
- Cholinephosphotransferase 1 (CHPT1), Choline/ethanolaminephosphotransferase 1 (CEPT1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.
GO - Molecular functioni
- diacylglycerol cholinephosphotransferase activity Source: GO_Central
- ethanolaminephosphotransferase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- phosphotransferase activity, for other substituted phosphate groups Source: GO_Central
GO - Biological processi
- lipid metabolic process Source: ProtInc
- phosphatidylcholine biosynthetic process Source: Reactome
- phosphatidylethanolamine biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Ligand | Magnesium, Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:HS05844-MONOMER |
PathwayCommonsi | Q9Y6K0 |
Reactomei | R-HSA-1483191, Synthesis of PC R-HSA-1483213, Synthesis of PE |
SABIO-RKi | Q9Y6K0 |
UniPathwayi | UPA00558;UER00743 UPA00753;UER00740 |
Protein family/group databases
TCDBi | 4.F.1.1.1, the choline/ethanolaminephosphotransferase 1 (cept1) family |
Chemistry databases
SwissLipidsi | SLP:000000137 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CEPT1Imported ORF Names:PRO1101 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24289, CEPT1 |
MIMi | 616751, gene |
neXtProti | NX_Q9Y6K0 |
VEuPathDBi | HostDB:ENSG00000134255.13 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication
Nucleus
- Nucleus membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: GO_Central
Nucleus
- nuclear membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: ProtInc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 87 – 107 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 115 – 135 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 186 – 206 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 209 – 229 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 239 – 259 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 283 – 303 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 317 – 337 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 365 – 385 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 138 | K → M: Induces a reduction in both cholinephosphotransferase and ethanolaminephosphotransferase activities. | 1 | |
Mutagenesisi | 144 | N → G: No effect. 1 Publication | 1 | |
Mutagenesisi | 146 | S → Q or C: No effect. 1 Publication | 1 | |
Mutagenesisi | 156 | G → C, S or A: Induces a reduction in cholinephosphotransferase activity and abolishes ethanolaminephosphotransferase activity. 1 Publication | 1 | |
Mutagenesisi | 214 | T → A: Alters the profile of diacylglycerol utilization and results in modest reduction in enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 215 | E → A or D: Induces a strong reduction in enzyme activity without altering diacylglycerol specificity. 1 Publication | 1 | |
Mutagenesisi | 215 | E → Q: Induces a strong reduction in enzyme activity and alters diacylglycerol specificity. 1 Publication | 1 | |
Mutagenesisi | 216 | V → A: Alters the profile of diacylglycerol utilization and results in modest reduction in enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 221 | I → A: Alters the profile of diacylglycerol utilization and results in modest reduction in enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 226 | L → A: Does not affect either the enzyme activity or the diacylglycerol specificity. 1 Publication | 1 | |
Mutagenesisi | 228 | V → A: Does not affect either the enzyme activity or the diacylglycerol specificity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10390 |
OpenTargetsi | ENSG00000134255 |
PharmGKBi | PA134892657 |
Miscellaneous databases
Pharosi | Q9Y6K0, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4105740 |
DrugBanki | DB00122, Choline DB14006, Choline salicylate |
Genetic variation databases
BioMutai | CEPT1 |
DMDMi | 74753524 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000289245 | 1 – 416 | Choline/ethanolaminephosphotransferase 1Add BLAST | 416 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 18 | PhosphoserineCombined sources | 1 | |
Modified residuei | 40 | PhosphothreonineCombined sources | 1 | |
Glycosylationi | 144 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q9Y6K0 |
jPOSTi | Q9Y6K0 |
MassIVEi | Q9Y6K0 |
MaxQBi | Q9Y6K0 |
PaxDbi | Q9Y6K0 |
PeptideAtlasi | Q9Y6K0 |
PRIDEi | Q9Y6K0 |
ProteomicsDBi | 86708 |
PTM databases
GlyGeni | Q9Y6K0, 1 site |
iPTMneti | Q9Y6K0 |
PhosphoSitePlusi | Q9Y6K0 |
SwissPalmi | Q9Y6K0 |
Expressioni
Tissue specificityi
Ubiquitously expressed.1 Publication
Gene expression databases
Bgeei | ENSG00000134255, Expressed in lymph node and 231 other tissues |
ExpressionAtlasi | Q9Y6K0, baseline and differential |
Genevisiblei | Q9Y6K0, HS |
Organism-specific databases
HPAi | ENSG00000134255, Low tissue specificity |
Interactioni
Binary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 115662, 43 interactors |
IntActi | Q9Y6K0, 39 interactors |
MINTi | Q9Y6K0 |
STRINGi | 9606.ENSP00000441980 |
Chemistry databases
BindingDBi | Q9Y6K0 |
Miscellaneous databases
RNActi | Q9Y6K0, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the CDP-alcohol phosphatidyltransferase class-I family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2877, Eukaryota |
GeneTreei | ENSGT00950000183117 |
HOGENOMi | CLU_035066_1_0_1 |
InParanoidi | Q9Y6K0 |
OMAi | EWYMVQG |
OrthoDBi | 847100at2759 |
PhylomeDBi | Q9Y6K0 |
TreeFami | TF313270 |
Family and domain databases
Gene3Di | 1.20.120.1760, 1 hit |
InterProi | View protein in InterPro IPR000462, CDP-OH_P_trans IPR043130, CDP-OH_PTrfase_TM_dom IPR014472, CHOPT |
PANTHERi | PTHR10414, PTHR10414, 1 hit |
Pfami | View protein in Pfam PF01066, CDP-OH_P_transf, 1 hit |
PIRSFi | PIRSF015665, CHOPT, 1 hit |
PROSITEi | View protein in PROSITE PS00379, CDP_ALCOHOL_P_TRANSF, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9Y6K0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGHRSTRKR CGDSHPESPV GFGHMSTTGC VLNKLFQLPT PPLSRHQLKR
60 70 80 90 100
LEEHRYQSAG RSLLEPLMQG YWEWLVRRVP SWIAPNLITI IGLSINICTT
110 120 130 140 150
ILLVFYCPTA TEQAPLWAYI ACACGLFIYQ SLDAIDGKQA RRTNSSSPLG
160 170 180 190 200
ELFDHGCDSL STVFVVLGTC IAVQLGTNPD WMFFCCFAGT FMFYCAHWQT
210 220 230 240 250
YVSGTLRFGI IDVTEVQIFI IIMHLLAVIG GPPFWQSMIP VLNIQMKIFP
260 270 280 290 300
ALCTVAGTIF SCTNYFRVIF TGGVGKNGST IAGTSVLSPF LHIGSVITLA
310 320 330 340 350
AMIYKKSAVQ LFEKHPCLYI LTFGFVSAKI TNKLVVAHMT KSEMHLHDTA
360 370 380 390 400
FIGPALLFLD QYFNSFIDEY IVLWIALVFS FFDLIRYCVS VCNQIASHLH
410
IHVFRIKVST AHSNHH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0C4DGS9 | A0A0C4DGS9_HUMAN | Choline/ethanolaminephosphotransfer... | CEPT1 | 280 | Annotation score: |
Sequence cautioni
The sequence AAF61194 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF068302 mRNA Translation: AAD25170.1 AF138862 mRNA Translation: AAF61194.1 Frameshift. AL355816 Genomic DNA No translation available. BC032610 mRNA Translation: AAH32610.1 BC049196 mRNA Translation: AAH49196.1 AL833102 mRNA Translation: CAH10403.1 |
CCDSi | CCDS830.1 |
RefSeqi | NP_001007795.1, NM_001007794.2 NP_001317672.1, NM_001330743.1 NP_006081.1, NM_006090.4 |
Genome annotation databases
Ensembli | ENST00000357172; ENSP00000349696; ENSG00000134255 ENST00000545121; ENSP00000441980; ENSG00000134255 |
GeneIDi | 10390 |
KEGGi | hsa:10390 |
UCSCi | uc001eah.1, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF068302 mRNA Translation: AAD25170.1 AF138862 mRNA Translation: AAF61194.1 Frameshift. AL355816 Genomic DNA No translation available. BC032610 mRNA Translation: AAH32610.1 BC049196 mRNA Translation: AAH49196.1 AL833102 mRNA Translation: CAH10403.1 |
CCDSi | CCDS830.1 |
RefSeqi | NP_001007795.1, NM_001007794.2 NP_001317672.1, NM_001330743.1 NP_006081.1, NM_006090.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 115662, 43 interactors |
IntActi | Q9Y6K0, 39 interactors |
MINTi | Q9Y6K0 |
STRINGi | 9606.ENSP00000441980 |
Chemistry databases
BindingDBi | Q9Y6K0 |
ChEMBLi | CHEMBL4105740 |
DrugBanki | DB00122, Choline DB14006, Choline salicylate |
SwissLipidsi | SLP:000000137 |
Protein family/group databases
TCDBi | 4.F.1.1.1, the choline/ethanolaminephosphotransferase 1 (cept1) family |
PTM databases
GlyGeni | Q9Y6K0, 1 site |
iPTMneti | Q9Y6K0 |
PhosphoSitePlusi | Q9Y6K0 |
SwissPalmi | Q9Y6K0 |
Genetic variation databases
BioMutai | CEPT1 |
DMDMi | 74753524 |
Proteomic databases
EPDi | Q9Y6K0 |
jPOSTi | Q9Y6K0 |
MassIVEi | Q9Y6K0 |
MaxQBi | Q9Y6K0 |
PaxDbi | Q9Y6K0 |
PeptideAtlasi | Q9Y6K0 |
PRIDEi | Q9Y6K0 |
ProteomicsDBi | 86708 |
Protocols and materials databases
Antibodypediai | 46938, 96 antibodies |
DNASUi | 10390 |
Genome annotation databases
Ensembli | ENST00000357172; ENSP00000349696; ENSG00000134255 ENST00000545121; ENSP00000441980; ENSG00000134255 |
GeneIDi | 10390 |
KEGGi | hsa:10390 |
UCSCi | uc001eah.1, human |
Organism-specific databases
CTDi | 10390 |
DisGeNETi | 10390 |
GeneCardsi | CEPT1 |
HGNCi | HGNC:24289, CEPT1 |
HPAi | ENSG00000134255, Low tissue specificity |
MIMi | 616751, gene |
neXtProti | NX_Q9Y6K0 |
OpenTargetsi | ENSG00000134255 |
PharmGKBi | PA134892657 |
VEuPathDBi | HostDB:ENSG00000134255.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2877, Eukaryota |
GeneTreei | ENSGT00950000183117 |
HOGENOMi | CLU_035066_1_0_1 |
InParanoidi | Q9Y6K0 |
OMAi | EWYMVQG |
OrthoDBi | 847100at2759 |
PhylomeDBi | Q9Y6K0 |
TreeFami | TF313270 |
Enzyme and pathway databases
UniPathwayi | UPA00558;UER00743 UPA00753;UER00740 |
BioCyci | MetaCyc:HS05844-MONOMER |
PathwayCommonsi | Q9Y6K0 |
Reactomei | R-HSA-1483191, Synthesis of PC R-HSA-1483213, Synthesis of PE |
SABIO-RKi | Q9Y6K0 |
Miscellaneous databases
BioGRID-ORCSi | 10390, 102 hits in 879 CRISPR screens |
ChiTaRSi | CEPT1, human |
GenomeRNAii | 10390 |
Pharosi | Q9Y6K0, Tbio |
PROi | PR:Q9Y6K0 |
RNActi | Q9Y6K0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000134255, Expressed in lymph node and 231 other tissues |
ExpressionAtlasi | Q9Y6K0, baseline and differential |
Genevisiblei | Q9Y6K0, HS |
Family and domain databases
Gene3Di | 1.20.120.1760, 1 hit |
InterProi | View protein in InterPro IPR000462, CDP-OH_P_trans IPR043130, CDP-OH_PTrfase_TM_dom IPR014472, CHOPT |
PANTHERi | PTHR10414, PTHR10414, 1 hit |
Pfami | View protein in Pfam PF01066, CDP-OH_P_transf, 1 hit |
PIRSFi | PIRSF015665, CHOPT, 1 hit |
PROSITEi | View protein in PROSITE PS00379, CDP_ALCOHOL_P_TRANSF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CEPT1_HUMAN | |
Accessioni | Q9Y6K0Primary (citable) accession number: Q9Y6K0 Secondary accession number(s): Q69YJ9, Q9P0Y8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 10, 2021 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families