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Protein

Epsin-1

Gene

EPN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei8Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei11Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei25Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei30Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei63Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei73Phosphatidylinositol lipid headgroupBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-HSA-182971 EGFR downregulation
R-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-HSA-8856828 Clathrin-mediated endocytosis
SignaLinkiQ9Y6I3
SIGNORiQ9Y6I3

Protein family/group databases

MoonDBiQ9Y6I3 Curated

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-1
Alternative name(s):
EH domain-binding mitotic phosphoprotein
EPS-15-interacting protein 1
Gene namesi
Name:EPN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000063245.14
HGNCiHGNC:21604 EPN1
MIMi607262 gene
neXtProtiNX_Q9Y6I3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Coated pit, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi382S → A: Abolishes phosphorylation by CDK1. 1 Publication1
Mutagenesisi382S → D: Abolishes phosphorylation by CDK1 and reduces REPS2 binding. 1 Publication1
Mutagenesisi404F → A: Reduces interaction with AP2B1. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000063245
PharmGKBiPA134860916

Chemistry databases

ChEMBLiCHEMBL3259465

Polymorphism and mutation databases

BioMutaiEPN1
DMDMi332278179

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745131 – 576Epsin-1Add BLAST576

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei382Phosphoserine; by CDK11 Publication1
Modified residuei419PhosphoserineCombined sources1
Modified residuei420PhosphoserineCombined sources1
Modified residuei435PhosphoserineCombined sources1
Modified residuei447PhosphoserineCombined sources1
Modified residuei454PhosphoserineCombined sources1
Modified residuei460PhosphothreonineCombined sources1
Modified residuei464PhosphothreonineCombined sources1
Modified residuei470PhosphothreonineCombined sources1
Modified residuei473PhosphoserineBy similarity1
Modified residuei494PhosphothreonineCombined sources1
Modified residuei534Omega-N-methylarginineBy similarity1

Post-translational modificationi

Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.1 Publication
Ubiquitinated.By similarity

Keywords - PTMi

Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Y6I3
MaxQBiQ9Y6I3
PeptideAtlasiQ9Y6I3
PRIDEiQ9Y6I3
ProteomicsDBi86689
86690 [Q9Y6I3-1]
86691 [Q9Y6I3-3]

PTM databases

iPTMnetiQ9Y6I3
PhosphoSitePlusiQ9Y6I3

Expressioni

Gene expression databases

BgeeiENSG00000063245 Expressed in 206 organ(s), highest expression level in body of stomach
CleanExiHS_EPN1
ExpressionAtlasiQ9Y6I3 baseline and differential
GenevisibleiQ9Y6I3 HS

Organism-specific databases

HPAiCAB009729
HPA061136

Interactioni

Subunit structurei

Monomer. Binds clathrin, ZBTB16/ZNF145 and ITSN1 (By similarity). Binds ubiquitinated proteins (By similarity). Binds REPS2, EPS15, AP2A1 and AP2A2. Interacts with RALBP1 in a complex also containing NUMB and TFAP2A during interphase and mitosis. Interacts with AP2B1. Interacts with UBQLN2.By similarity5 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi118965, 69 interactors
CORUMiQ9Y6I3
ELMiQ9Y6I3
IntActiQ9Y6I3, 25 interactors
MINTiQ9Y6I3

Chemistry databases

BindingDBiQ9Y6I3

Structurei

Secondary structure

1576
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y6I3
SMRiQ9Y6I3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y6I3

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 144ENTHPROSITE-ProRule annotationAdd BLAST133
Domaini183 – 202UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini208 – 227UIM 2PROSITE-ProRule annotationAdd BLAST20
Domaini233 – 252UIM 3PROSITE-ProRule annotationAdd BLAST20
Repeati274 – 27613
Repeati294 – 29623
Repeati306 – 30833
Repeati319 – 32143
Repeati332 – 33453
Repeati349 – 35163
Repeati367 – 36973
Repeati377 – 37983
Repeati502 – 50413
Repeati518 – 52023
Repeati572 – 57433

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni274 – 3798 X 3 AA repeats of [ED]-P-WAdd BLAST106
Regioni502 – 5743 X 3 AA repeats of N-P-FAdd BLAST73

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi402 – 411[DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi267 – 573Ala/Gly/Pro-richAdd BLAST307

Domaini

The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP2A2 and clathrin binding.
The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction with the AP-2 complex subunit AP2B1.By similarity

Sequence similaritiesi

Belongs to the epsin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074611
HOGENOMiHOG000008298
HOVERGENiHBG006690
InParanoidiQ9Y6I3
KOiK12471
OMAiITNPFQP
OrthoDBiEOG091G0L45
TreeFamiTF313361

Family and domain databases

CDDicd03571 ENTH_epsin, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR039416 Epsin_ENTH
IPR003903 UIM_dom
PfamiView protein in Pfam
PF01417 ENTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SM00726 UIM, 3 hits
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
PS50330 UIM, 3 hits

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9Y6I3-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSTSSLRRQM KNIVHNYSEA EIKVREATSN DPWGPSSSLM SEIADLTYNV
60 70 80 90 100
VAFSEIMSMI WKRLNDHGKN WRHVYKAMTL MEYLIKTGSE RVSQQCKENM
110 120 130 140 150
YAVQTLKDFQ YVDRDGKDQG VNVREKAKQL VALLRDEDRL REERAHALKT
160 170 180 190 200
KEKLAQTATA SSAAVGSGPP PEAEQAWPQS SGEEELQLQL ALAMSKEEAD
210 220 230 240 250
QPPSCGPEDD AQLQLALSLS REEHDKEERI RRGDDLRLQM AIEESKRETG
260 270 280 290 300
GKEESSLMDL ADVFTAPAPA PTTDPWGGPA PMAAAVPTAA PTSDPWGGPP
310 320 330 340 350
VPPAADPWGG PAPTPASGDP WRPAAPAGPS VDPWGGTPAP AAGEGPTPDP
360 370 380 390 400
WGSSDGGVPV SGPSASDPWT PAPAFSDPWG GSPAKPSTNG TTAAGGFDTE
410 420 430 440 450
PDEFSDFDRL RTALPTSGSS AGELELLAGE VPARSPGAFD MSGVRGSLAE
460 470 480 490 500
AVGSPPPAAT PTPTPPTRKT PESFLGPNAA LVDLDSLVSR PGPTPPGAKA
510 520 530 540 550
SNPFLPGGGP ATGPSVTNPF QPAPPATLTL NQLRLSPVPP VPGAPPTYIS
560 570
PLGGGPGLPP MMPPGPPAPN TNPFLL
Length:576
Mass (Da):60,293
Last modified:May 3, 2011 - v2
Checksum:i68DD433F3168E975
GO
Isoform 2 (identifier: Q9Y6I3-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGDQSWLWNQ...CPLLLQPGTM
     202-226: Missing.

Show »
Length:662
Mass (Da):69,040
Checksum:iBE8DD37499E2DB6A
GO
Isoform 3 (identifier: Q9Y6I3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     202-226: Missing.
     393-393: Missing.

Note: May be due to a competing donor splice site. No experimental confirmation available.
Show »
Length:550
Mass (Da):57,504
Checksum:iDC8BA5EBEB1D5111
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7EMP4K7EMP4_HUMAN
Epsin-1
EPN1
60Annotation score:

Sequence cautioni

Isoform 2 : The sequence AAD38326 differs from that shown. Reason: Frameshift at position 98.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0410101M → MGDQSWLWNQAAPGVRSPVF ACSVEKGNVPLVLSEHLAHS RDPGSGAVRFLISPEPWASA ILGTSGLLASPVLPAALDAV TCQHLPQPSSGSRPISPRIG ALCPLLLQPGTM in isoform 2. 1 Publication1
Alternative sequenceiVSP_041011202 – 226Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST25
Alternative sequenceiVSP_041012393Missing in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073727 mRNA Translation: AAD38326.1 Frameshift.
AK022454 mRNA Translation: BAB14041.1
AC008735 Genomic DNA No translation available.
AC010525 Genomic DNA No translation available.
BC044651 mRNA Translation: AAH44651.1
CCDSiCCDS46198.1 [Q9Y6I3-1]
CCDS46199.1 [Q9Y6I3-2]
CCDS46200.1 [Q9Y6I3-3]
RefSeqiNP_001123543.1, NM_001130071.1 [Q9Y6I3-1]
NP_001123544.1, NM_001130072.1 [Q9Y6I3-2]
NP_037465.2, NM_013333.3 [Q9Y6I3-3]
XP_005258886.1, XM_005258829.2 [Q9Y6I3-2]
XP_011525183.1, XM_011526881.1
XP_016882211.1, XM_017026722.1 [Q9Y6I3-3]
UniGeneiHs.279953

Genome annotation databases

EnsembliENST00000085079; ENSP00000085079; ENSG00000063245 [Q9Y6I3-3]
ENST00000270460; ENSP00000270460; ENSG00000063245 [Q9Y6I3-2]
ENST00000411543; ENSP00000406209; ENSG00000063245 [Q9Y6I3-1]
GeneIDi29924
KEGGihsa:29924
UCSCiuc002qlv.4 human [Q9Y6I3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Protein Spotlight

The bubble's bend - Issue 42 of January 2004

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073727 mRNA Translation: AAD38326.1 Frameshift.
AK022454 mRNA Translation: BAB14041.1
AC008735 Genomic DNA No translation available.
AC010525 Genomic DNA No translation available.
BC044651 mRNA Translation: AAH44651.1
CCDSiCCDS46198.1 [Q9Y6I3-1]
CCDS46199.1 [Q9Y6I3-2]
CCDS46200.1 [Q9Y6I3-3]
RefSeqiNP_001123543.1, NM_001130071.1 [Q9Y6I3-1]
NP_001123544.1, NM_001130072.1 [Q9Y6I3-2]
NP_037465.2, NM_013333.3 [Q9Y6I3-3]
XP_005258886.1, XM_005258829.2 [Q9Y6I3-2]
XP_011525183.1, XM_011526881.1
XP_016882211.1, XM_017026722.1 [Q9Y6I3-3]
UniGeneiHs.279953

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1INZNMR-A1-144[»]
1KYDX-ray2.00P366-370[»]
ProteinModelPortaliQ9Y6I3
SMRiQ9Y6I3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118965, 69 interactors
CORUMiQ9Y6I3
ELMiQ9Y6I3
IntActiQ9Y6I3, 25 interactors
MINTiQ9Y6I3

Chemistry databases

BindingDBiQ9Y6I3
ChEMBLiCHEMBL3259465

Protein family/group databases

MoonDBiQ9Y6I3 Curated

PTM databases

iPTMnetiQ9Y6I3
PhosphoSitePlusiQ9Y6I3

Polymorphism and mutation databases

BioMutaiEPN1
DMDMi332278179

Proteomic databases

EPDiQ9Y6I3
MaxQBiQ9Y6I3
PeptideAtlasiQ9Y6I3
PRIDEiQ9Y6I3
ProteomicsDBi86689
86690 [Q9Y6I3-1]
86691 [Q9Y6I3-3]

Protocols and materials databases

DNASUi29924
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000085079; ENSP00000085079; ENSG00000063245 [Q9Y6I3-3]
ENST00000270460; ENSP00000270460; ENSG00000063245 [Q9Y6I3-2]
ENST00000411543; ENSP00000406209; ENSG00000063245 [Q9Y6I3-1]
GeneIDi29924
KEGGihsa:29924
UCSCiuc002qlv.4 human [Q9Y6I3-2]

Organism-specific databases

CTDi29924
EuPathDBiHostDB:ENSG00000063245.14
GeneCardsiEPN1
H-InvDBiHIX0202721
HGNCiHGNC:21604 EPN1
HPAiCAB009729
HPA061136
MIMi607262 gene
neXtProtiNX_Q9Y6I3
OpenTargetsiENSG00000063245
PharmGKBiPA134860916
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00550000074611
HOGENOMiHOG000008298
HOVERGENiHBG006690
InParanoidiQ9Y6I3
KOiK12471
OMAiITNPFQP
OrthoDBiEOG091G0L45
TreeFamiTF313361

Enzyme and pathway databases

ReactomeiR-HSA-182971 EGFR downregulation
R-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-HSA-8856828 Clathrin-mediated endocytosis
SignaLinkiQ9Y6I3
SIGNORiQ9Y6I3

Miscellaneous databases

EvolutionaryTraceiQ9Y6I3
GeneWikiiEPN1
GenomeRNAii29924
PROiPR:Q9Y6I3
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000063245 Expressed in 206 organ(s), highest expression level in body of stomach
CleanExiHS_EPN1
ExpressionAtlasiQ9Y6I3 baseline and differential
GenevisibleiQ9Y6I3 HS

Family and domain databases

CDDicd03571 ENTH_epsin, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR039416 Epsin_ENTH
IPR003903 UIM_dom
PfamiView protein in Pfam
PF01417 ENTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SM00726 UIM, 3 hits
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
PS50330 UIM, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiEPN1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6I3
Secondary accession number(s): Q86ST3, Q9HA18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: May 3, 2011
Last modified: November 7, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
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