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Protein

Serine/threonine-protein kinase 24

Gene

STK24

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. In association with STK26 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation (PubMed:27807006). Regulates also cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve.6 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Co2+1 Publication, Zn2+1 Publication

Kineticsi

  1. KM=12.8 µM for manganese ions1 Publication
  2. KM=34.9 µM for cobalt ions1 Publication
  3. KM=22.7 µM for magnesium ions1 Publication
  4. KM=4.1 µM for zinc ions1 Publication
  1. Vmax=2623.1 pmol/min/mg enzyme for manganese ions1 Publication
  2. Vmax=1746.1 pmol/min/mg enzyme for cobalt ions1 Publication
  3. Vmax=129.1 pmol/min/mg enzyme for magnesium ions1 Publication
  4. Vmax=22.3 pmol/min/mg enzyme for zinc ions1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65ATP1
Active sitei156Proton acceptorPROSITE-ProRule annotation1
Metal bindingi161Magnesium1
Metal bindingi174Magnesium1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 50ATP9
Nucleotide bindingi112 – 114ATP3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cadherin binding Source: BHF-UCL
  • metal ion binding Source: UniProtKB-KW
  • protein kinase activity Source: ProtInc
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-111465 Apoptotic cleavage of cellular proteins
R-HSA-75153 Apoptotic execution phase
SignaLinkiQ9Y6E0
SIGNORiQ9Y6E0

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 24 (EC:2.7.11.1)
Alternative name(s):
Mammalian STE20-like protein kinase 3
Short name:
MST-3
STE20-like kinase MST3
Cleaved into the following 2 chains:
Alternative name(s):
Mammalian STE20-like protein kinase 3 N-terminal
Short name:
MST3/N
Alternative name(s):
Mammalian STE20-like protein kinase 3 C-terminal
Short name:
MST3/C
Gene namesi
Name:STK24
Synonyms:MST3, STK3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000102572.14
HGNCiHGNC:11403 STK24
MIMi604984 gene
neXtProtiNX_Q9Y6E0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi18T → A: Loss of phosphorylation by PKA. 1 Publication1
Mutagenesisi65K → A: Loss of activity and autophosphorylation. 1 Publication1
Mutagenesisi190T → A: Loss of activity and autophosphorylation. 1 Publication1
Mutagenesisi321D → N: Loss of proteolytic cleavage by caspases. 1 Publication1

Organism-specific databases

DisGeNETi8428
OpenTargetsiENSG00000102572
PharmGKBiPA36210

Chemistry databases

ChEMBLiCHEMBL5082
GuidetoPHARMACOLOGYi2217

Polymorphism and mutation databases

DMDMi13626607

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867111 – 443Serine/threonine-protein kinase 24Add BLAST443
ChainiPRO_00004136181 – 325Serine/threonine-protein kinase 24 36 kDa subunitAdd BLAST325
ChainiPRO_0000413619326 – 443Serine/threonine-protein kinase 24 12 kDa subunitAdd BLAST118
Isoform A (identifier: Q9Y6E0-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18Phosphothreonine; by PKA1 Publication1
Modified residuei190Phosphothreonine; by autocatalysisCombined sources3 Publications1
Modified residuei320PhosphoserineCombined sources1
Isoform A (identifier: Q9Y6E0-2)
Modified residuei2N-acetylalanineCombined sources1
Modified residuei4PhosphoserineCombined sources1

Post-translational modificationi

Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.1 Publication
Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei325 – 326Cleavage; by caspase-3, caspase-7 and caspase-82

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Y6E0
PaxDbiQ9Y6E0
PeptideAtlasiQ9Y6E0
PRIDEiQ9Y6E0
ProteomicsDBi86657
86658 [Q9Y6E0-2]

PTM databases

iPTMnetiQ9Y6E0
PhosphoSitePlusiQ9Y6E0
SwissPalmiQ9Y6E0

Expressioni

Tissue specificityi

Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex.

Gene expression databases

BgeeiENSG00000102572 Expressed in 237 organ(s), highest expression level in secondary oocyte
CleanExiHS_STK24
HS_STK3
ExpressionAtlasiQ9Y6E0 baseline and differential
GenevisibleiQ9Y6E0 HS

Organism-specific databases

HPAiHPA026435
HPA026502

Interactioni

Subunit structurei

Monomer (PubMed:20124694). Interacts with CTTNBP2NL (PubMed:18782753). Interacts with RIPOR1 (via C-terminus); this interaction occurs in a PDCD10-dependent and Rho-independent manner (PubMed:27807006). Interacts with PDCD10; this interaction is required for the association of STK24 with RIPOR1 (PubMed:27807006).3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114011, 79 interactors
DIPiDIP-40608N
IntActiQ9Y6E0, 37 interactors
MINTiQ9Y6E0
STRINGi9606.ENSP00000365730

Chemistry databases

BindingDBiQ9Y6E0

Structurei

Secondary structure

1443
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y6E0
SMRiQ9Y6E0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y6E0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 286Protein kinasePROSITE-ProRule annotationAdd BLAST251

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi278 – 292Bipartite nuclear localization signalAdd BLAST15
Motifi335 – 386Nuclear export signal (NES)Add BLAST52

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0201 Eukaryota
ENOG410XP9G LUCA
GeneTreeiENSGT00930000150827
HOGENOMiHOG000234203
HOVERGENiHBG108518
InParanoidiQ9Y6E0
KOiK08838
OMAiDTDGQAS
OrthoDBiEOG091G0F24
PhylomeDBiQ9Y6E0
TreeFamiTF354217

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform B (identifier: Q9Y6E0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDSRAQLWGL ALNKRRATLP HPGGSTNLKA DPEELFTKLE KIGKGSFGEV
60 70 80 90 100
FKGIDNRTQK VVAIKIIDLE EAEDEIEDIQ QEITVLSQCD SPYVTKYYGS
110 120 130 140 150
YLKDTKLWII MEYLGGGSAL DLLEPGPLDE TQIATILREI LKGLDYLHSE
160 170 180 190 200
KKIHRDIKAA NVLLSEHGEV KLADFGVAGQ LTDTQIKRNT FVGTPFWMAP
210 220 230 240 250
EVIKQSAYDS KADIWSLGIT AIELARGEPP HSELHPMKVL FLIPKNNPPT
260 270 280 290 300
LEGNYSKPLK EFVEACLNKE PSFRPTAKEL LKHKFILRNA KKTSYLTELI
310 320 330 340 350
DRYKRWKAEQ SHDDSSSEDS DAETDGQASG GSDSGDWIFT IREKDPKNLE
360 370 380 390 400
NGALQPSDLD RNKMKDIPKR PFSQCLSTII SPLFAELKEK SQACGGNLGS
410 420 430 440
IEELRGAIYL AEEACPGISD TMVAQLVQRL QRYSLSGGGT SSH
Length:443
Mass (Da):49,308
Last modified:November 1, 1999 - v1
Checksum:i4A9FF1F6B6A88A97
GO
Isoform A (identifier: Q9Y6E0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: MDSRAQLWGLALNKRRATLPHPGGST → MAHSPVQSGLPGMQ

Show »
Length:431
Mass (Da):47,913
Checksum:i26D863FDFEDF90AD
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B4DR80B4DR80_HUMAN
Serine/threonine-protein kinase 24
STK24
412Annotation score:
Q5JV98Q5JV98_HUMAN
Serine/threonine-protein kinase 24
STK24
209Annotation score:
H0Y630H0Y630_HUMAN
Serine/threonine-protein kinase 24
STK24
369Annotation score:
Q5JV99Q5JV99_HUMAN
Serine/threonine-protein kinase 24
STK24
142Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_041148414A → V2 PublicationsCorresponds to variant dbSNP:rs55953606Ensembl.1
Natural variantiVAR_041149426L → I1 PublicationCorresponds to variant dbSNP:rs55897869Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0048741 – 26MDSRA…PGGST → MAHSPVQSGLPGMQ in isoform A. 2 PublicationsAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024636 mRNA Translation: AAB82560.1
AF083420 mRNA Translation: AAD42039.1
AL356423 Genomic DNA No translation available.
AL137249 Genomic DNA No translation available.
CH471085 Genomic DNA Translation: EAX08986.1
BC035578 mRNA Translation: AAH35578.1
CCDSiCCDS32001.1 [Q9Y6E0-2]
CCDS9488.1 [Q9Y6E0-1]
RefSeqiNP_001027467.2, NM_001032296.3 [Q9Y6E0-2]
NP_003567.2, NM_003576.4 [Q9Y6E0-1]
UniGeneiHs.508514

Genome annotation databases

EnsembliENST00000376547; ENSP00000365730; ENSG00000102572 [Q9Y6E0-1]
ENST00000539966; ENSP00000442539; ENSG00000102572 [Q9Y6E0-2]
GeneIDi8428
KEGGihsa:8428
UCSCiuc001vnm.3 human [Q9Y6E0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024636 mRNA Translation: AAB82560.1
AF083420 mRNA Translation: AAD42039.1
AL356423 Genomic DNA No translation available.
AL137249 Genomic DNA No translation available.
CH471085 Genomic DNA Translation: EAX08986.1
BC035578 mRNA Translation: AAH35578.1
CCDSiCCDS32001.1 [Q9Y6E0-2]
CCDS9488.1 [Q9Y6E0-1]
RefSeqiNP_001027467.2, NM_001032296.3 [Q9Y6E0-2]
NP_003567.2, NM_003576.4 [Q9Y6E0-1]
UniGeneiHs.508514

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A7FX-ray1.55A27-315[»]
3A7GX-ray2.00A/B27-315[»]
3A7HX-ray1.96A/B27-315[»]
3A7IX-ray1.45A27-315[»]
3A7JX-ray1.50A27-315[»]
3CKWX-ray1.96A31-323[»]
3CKXX-ray2.70A31-323[»]
3ZHPX-ray2.90C/D31-301[»]
4O27X-ray3.19B30-309[»]
4QMLX-ray1.88A24-315[»]
4QMMX-ray1.85A24-315[»]
4QMNX-ray2.09A27-315[»]
4QMOX-ray1.90A24-315[»]
4QMPX-ray2.00A24-315[»]
4QMQX-ray1.77A24-315[»]
4QMSX-ray1.88A24-315[»]
4QMTX-ray1.50A24-315[»]
4QMUX-ray1.55A24-315[»]
4QMVX-ray2.40A24-315[»]
4QMWX-ray1.60A24-315[»]
4QMXX-ray1.88A24-315[»]
4QMYX-ray1.88A24-315[»]
4QMZX-ray1.88A24-315[»]
4QNAX-ray1.85A27-315[»]
4QO9X-ray2.20A/B24-315[»]
4U8ZX-ray1.63A24-310[»]
4W8DX-ray1.77A24-310[»]
4W8EX-ray1.79A24-311[»]
ProteinModelPortaliQ9Y6E0
SMRiQ9Y6E0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114011, 79 interactors
DIPiDIP-40608N
IntActiQ9Y6E0, 37 interactors
MINTiQ9Y6E0
STRINGi9606.ENSP00000365730

Chemistry databases

BindingDBiQ9Y6E0
ChEMBLiCHEMBL5082
GuidetoPHARMACOLOGYi2217

PTM databases

iPTMnetiQ9Y6E0
PhosphoSitePlusiQ9Y6E0
SwissPalmiQ9Y6E0

Polymorphism and mutation databases

DMDMi13626607

Proteomic databases

EPDiQ9Y6E0
PaxDbiQ9Y6E0
PeptideAtlasiQ9Y6E0
PRIDEiQ9Y6E0
ProteomicsDBi86657
86658 [Q9Y6E0-2]

Protocols and materials databases

DNASUi8428
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000376547; ENSP00000365730; ENSG00000102572 [Q9Y6E0-1]
ENST00000539966; ENSP00000442539; ENSG00000102572 [Q9Y6E0-2]
GeneIDi8428
KEGGihsa:8428
UCSCiuc001vnm.3 human [Q9Y6E0-1]

Organism-specific databases

CTDi8428
DisGeNETi8428
EuPathDBiHostDB:ENSG00000102572.14
GeneCardsiSTK24
HGNCiHGNC:11403 STK24
HPAiHPA026435
HPA026502
MIMi604984 gene
neXtProtiNX_Q9Y6E0
OpenTargetsiENSG00000102572
PharmGKBiPA36210
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0201 Eukaryota
ENOG410XP9G LUCA
GeneTreeiENSGT00930000150827
HOGENOMiHOG000234203
HOVERGENiHBG108518
InParanoidiQ9Y6E0
KOiK08838
OMAiDTDGQAS
OrthoDBiEOG091G0F24
PhylomeDBiQ9Y6E0
TreeFamiTF354217

Enzyme and pathway databases

ReactomeiR-HSA-111465 Apoptotic cleavage of cellular proteins
R-HSA-75153 Apoptotic execution phase
SignaLinkiQ9Y6E0
SIGNORiQ9Y6E0

Miscellaneous databases

ChiTaRSiSTK24 human
EvolutionaryTraceiQ9Y6E0
GeneWikiiSTK24
GenomeRNAii8428
PROiPR:Q9Y6E0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000102572 Expressed in 237 organ(s), highest expression level in secondary oocyte
CleanExiHS_STK24
HS_STK3
ExpressionAtlasiQ9Y6E0 baseline and differential
GenevisibleiQ9Y6E0 HS

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSTK24_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6E0
Secondary accession number(s): O14840, Q5JV92
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 181 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  7. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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