UniProtKB - Q9Y6B2 (EID1_HUMAN)
Protein
EP300-interacting inhibitor of differentiation 1
Gene
EID1
Organism
Homo sapiens (Human)
Status
Functioni
Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.2 Publications
Miscellaneous
Inhibition of MYOD1 may be partly due to the ability of EID1 to bind and inhibit EP300 histone acetyltransferase activity.1 Publication
GO - Molecular functioni
- histone acetyltransferase binding Source: UniProtKB
- histone acetyltransferase regulator activity Source: UniProtKB
- transcription corepressor activity Source: UniProtKB
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell differentiation Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: MGI
- regulation of cell differentiation Source: InterPro
Keywordsi
Molecular function | Repressor |
Biological process | Cell cycle, Differentiation, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q9Y6B2 |
SIGNORi | Q9Y6B2 |
Names & Taxonomyi
Protein namesi | Recommended name: EP300-interacting inhibitor of differentiation 1Alternative name(s): 21 kDa pRb-associated protein CREBBP/EP300 inhibitory protein 1 E1A-like inhibitor of differentiation 1 Short name: EID-1 |
Gene namesi | ORF Names:PNAS-22, PTD014 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1191, EID1 |
MIMi | 605894, gene |
neXtProti | NX_Q9Y6B2 |
VEuPathDBi | HostDB:ENSG00000255302.3 |
Subcellular locationi
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: MGI
Other locations
- cytoplasmic ribonucleoprotein granule Source: HPA
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 178 | L → S: Abolishes RB1 binding. 1 Publication | 1 | |
Mutagenesisi | 180 | C → G: Abolishes RB1 binding. 2 Publications | 1 | |
Mutagenesisi | 182 | E → Q: Abolishes RB1 binding. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 23741 |
OpenTargetsi | ENSG00000255302 |
PharmGKBi | PA26876 |
Miscellaneous databases
Pharosi | Q9Y6B2, Tbio |
Genetic variation databases
BioMutai | EID1 |
DMDMi | 74721525 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000289156 | 1 – 187 | EP300-interacting inhibitor of differentiation 1Add BLAST | 187 |
Post-translational modificationi
Ubiquitinated in U2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.1 Publication
Keywords - PTMi
Ubl conjugationProteomic databases
MassIVEi | Q9Y6B2 |
PaxDbi | Q9Y6B2 |
PeptideAtlasi | Q9Y6B2 |
PRIDEi | Q9Y6B2 |
ProteomicsDBi | 86644 [Q9Y6B2-1] 86645 [Q9Y6B2-2] |
PTM databases
iPTMneti | Q9Y6B2 |
PhosphoSitePlusi | Q9Y6B2 |
Expressioni
Tissue specificityi
Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A-549 and various leukemia cell lines.2 Publications
Developmental stagei
Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level).1 Publication
Inductioni
Down-regulated in differentiating U-937 leukemia cells.1 Publication
Gene expression databases
Bgeei | ENSG00000255302, Expressed in tendon of biceps brachii and 247 other tissues |
ExpressionAtlasi | Q9Y6B2, baseline and differential |
Genevisiblei | Q9Y6B2, HS |
Organism-specific databases
HPAi | ENSG00000255302, Low tissue specificity |
Interactioni
Subunit structurei
Interacts via its LXCXE motif with the entire pocket region of RB1.
Interacts with EP300, NR0B2 and TRIM27.
By similarity4 PublicationsBinary interactionsi
Hide detailsQ9Y6B2
With | #Exp. | IntAct |
---|---|---|
NDN [Q99608] | 2 | EBI-1049975,EBI-718177 |
Ndn [P25233] from Mus musculus. | 5 | EBI-1049975,EBI-1801080 |
GO - Molecular functioni
- histone acetyltransferase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 117243, 48 interactors |
ELMi | Q9Y6B2 |
IntActi | Q9Y6B2, 22 interactors |
MINTi | Q9Y6B2 |
STRINGi | 9606.ENSP00000431162 |
Miscellaneous databases
RNActi | Q9Y6B2, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 54 – 120 | Interaction with NR0B2By similarityAdd BLAST | 67 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 178 – 182 | LXCXE motif1 Publication | 5 |
Phylogenomic databases
eggNOGi | ENOG502RQIS, Eukaryota |
GeneTreei | ENSGT00940000154796 |
InParanoidi | Q9Y6B2 |
OMAi | ESNELQM |
OrthoDBi | 1497086at2759 |
PhylomeDBi | Q9Y6B2 |
TreeFami | TF337633 |
Family and domain databases
InterProi | View protein in InterPro IPR033258, EID IPR033255, EID-1 |
PANTHERi | PTHR15556, PTHR15556, 1 hit PTHR15556:SF5, PTHR15556:SF5, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 14 Publications (identifier: Q9Y6B2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSEMAELSEL YEESSDLQMD VMPGEGDLPQ MEVGSGSREL SLRPSRSGAQ
60 70 80 90 100
QLEEEGPMEE EEAQPMAAPE GKRSLANGPN AGEQPGQVAG ADFESEDEGE
110 120 130 140 150
EFDDWEDDYD YPEEEQLSGA GYRVSAALEE ADKMFLRTRE PALDGGFQMH
160 170 180
YEKTPFDQLA FIEELFSLMV VNRLTEELGC DEIIDRE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH0YN68 | H0YN68_HUMAN | EP300-interacting inhibitor of diff... | EID1 | 165 | Annotation score: |
Sequence cautioni
The sequence AAK07524 differs from that shown. Reason: Erroneous initiation.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_052454 | 65 – 87 | Missing in isoform 2. 1 PublicationAdd BLAST | 23 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF349444 mRNA Translation: AAK29640.1 AF109873 mRNA Translation: AAG35179.1 AF092135 mRNA Translation: AAD40377.1 AL109701 mRNA Translation: CAB52022.1 AL357456 mRNA Translation: CAB93108.1 AK098383 mRNA Translation: BAC05296.1 AK315365 mRNA Translation: BAG37758.1 CH471082 Genomic DNA Translation: EAW77357.1 BC114944 mRNA Translation: AAI14945.1 BC114946 mRNA Translation: AAI14947.1 AF274947 mRNA Translation: AAK07524.1 Different initiation. |
CCDSi | CCDS53941.1 [Q9Y6B2-1] |
RefSeqi | NP_055150.1, NM_014335.2 [Q9Y6B2-1] |
Genome annotation databases
Ensembli | ENST00000530028; ENSP00000431162; ENSG00000255302 [Q9Y6B2-1] |
GeneIDi | 23741 |
KEGGi | hsa:23741 |
UCSCi | uc001zxc.2, human [Q9Y6B2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF349444 mRNA Translation: AAK29640.1 AF109873 mRNA Translation: AAG35179.1 AF092135 mRNA Translation: AAD40377.1 AL109701 mRNA Translation: CAB52022.1 AL357456 mRNA Translation: CAB93108.1 AK098383 mRNA Translation: BAC05296.1 AK315365 mRNA Translation: BAG37758.1 CH471082 Genomic DNA Translation: EAW77357.1 BC114944 mRNA Translation: AAI14945.1 BC114946 mRNA Translation: AAI14947.1 AF274947 mRNA Translation: AAK07524.1 Different initiation. |
CCDSi | CCDS53941.1 [Q9Y6B2-1] |
RefSeqi | NP_055150.1, NM_014335.2 [Q9Y6B2-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 117243, 48 interactors |
ELMi | Q9Y6B2 |
IntActi | Q9Y6B2, 22 interactors |
MINTi | Q9Y6B2 |
STRINGi | 9606.ENSP00000431162 |
PTM databases
iPTMneti | Q9Y6B2 |
PhosphoSitePlusi | Q9Y6B2 |
Genetic variation databases
BioMutai | EID1 |
DMDMi | 74721525 |
Proteomic databases
MassIVEi | Q9Y6B2 |
PaxDbi | Q9Y6B2 |
PeptideAtlasi | Q9Y6B2 |
PRIDEi | Q9Y6B2 |
ProteomicsDBi | 86644 [Q9Y6B2-1] 86645 [Q9Y6B2-2] |
Protocols and materials databases
Antibodypediai | 53733, 136 antibodies |
Genome annotation databases
Ensembli | ENST00000530028; ENSP00000431162; ENSG00000255302 [Q9Y6B2-1] |
GeneIDi | 23741 |
KEGGi | hsa:23741 |
UCSCi | uc001zxc.2, human [Q9Y6B2-1] |
Organism-specific databases
CTDi | 23741 |
DisGeNETi | 23741 |
GeneCardsi | EID1 |
HGNCi | HGNC:1191, EID1 |
HPAi | ENSG00000255302, Low tissue specificity |
MIMi | 605894, gene |
neXtProti | NX_Q9Y6B2 |
OpenTargetsi | ENSG00000255302 |
PharmGKBi | PA26876 |
VEuPathDBi | HostDB:ENSG00000255302.3 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502RQIS, Eukaryota |
GeneTreei | ENSGT00940000154796 |
InParanoidi | Q9Y6B2 |
OMAi | ESNELQM |
OrthoDBi | 1497086at2759 |
PhylomeDBi | Q9Y6B2 |
TreeFami | TF337633 |
Enzyme and pathway databases
PathwayCommonsi | Q9Y6B2 |
SIGNORi | Q9Y6B2 |
Miscellaneous databases
BioGRID-ORCSi | 23741, 5 hits in 999 CRISPR screens |
ChiTaRSi | EID1, human |
GeneWikii | EID1 |
GenomeRNAii | 23741 |
Pharosi | Q9Y6B2, Tbio |
PROi | PR:Q9Y6B2 |
RNActi | Q9Y6B2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000255302, Expressed in tendon of biceps brachii and 247 other tissues |
ExpressionAtlasi | Q9Y6B2, baseline and differential |
Genevisiblei | Q9Y6B2, HS |
Family and domain databases
InterProi | View protein in InterPro IPR033258, EID IPR033255, EID-1 |
PANTHERi | PTHR15556, PTHR15556, 1 hit PTHR15556:SF5, PTHR15556:SF5, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EID1_HUMAN | |
Accessioni | Q9Y6B2Primary (citable) accession number: Q9Y6B2 Secondary accession number(s): B2RD11, Q8N7I4, Q9BZT9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | November 1, 1999 | |
Last modified: | April 7, 2021 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot