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Protein

EP300-interacting inhibitor of differentiation 1

Gene

EID1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.2 Publications

Miscellaneous

Inhibition of MYOD1 may be partly due to the ability of EID1 to bind and inhibit EP300 histone acetyltransferase activity.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor
Biological processCell cycle, Differentiation, Transcription, Transcription regulation

Enzyme and pathway databases

SIGNORiQ9Y6B2

Names & Taxonomyi

Protein namesi
Recommended name:
EP300-interacting inhibitor of differentiation 1
Alternative name(s):
21 kDa pRb-associated protein
CREBBP/EP300 inhibitory protein 1
E1A-like inhibitor of differentiation 1
Short name:
EID-1
Gene namesi
Name:EID1Imported
Synonyms:C15orf3, CRI1Imported, RBP21
ORF Names:PNAS-22, PTD014
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000255302.3
HGNCiHGNC:1191 EID1
MIMi605894 gene
neXtProtiNX_Q9Y6B2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi178L → S: Abolishes RB1 binding. 1 Publication1
Mutagenesisi180C → G: Abolishes RB1 binding. 2 Publications1
Mutagenesisi182E → Q: Abolishes RB1 binding. 1 Publication1

Organism-specific databases

DisGeNETi23741
OpenTargetsiENSG00000255302
PharmGKBiPA26876

Polymorphism and mutation databases

BioMutaiEID1
DMDMi74721525

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002891561 – 187EP300-interacting inhibitor of differentiation 1Add BLAST187

Post-translational modificationi

Ubiquitinated in U2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9Y6B2
PeptideAtlasiQ9Y6B2
PRIDEiQ9Y6B2
ProteomicsDBi86644
86645 [Q9Y6B2-2]

PTM databases

iPTMnetiQ9Y6B2
PhosphoSitePlusiQ9Y6B2

Expressioni

Tissue specificityi

Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A-549 and various leukemia cell lines.2 Publications

Developmental stagei

Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level).1 Publication

Inductioni

Down-regulated in differentiating U-937 leukemia cells.1 Publication

Gene expression databases

BgeeiENSG00000255302 Expressed in 236 organ(s), highest expression level in tendon of biceps brachii
CleanExiHS_EID1
ExpressionAtlasiQ9Y6B2 baseline and differential
GenevisibleiQ9Y6B2 HS

Organism-specific databases

HPAiHPA051122
HPA051123

Interactioni

Subunit structurei

Interacts via its LXCXE motif with the entire pocket region of RB1. Interacts with EP300, NR0B2 and TRIM27.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NdnP252335EBI-1049975,EBI-1801080From Mus musculus.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi117243, 48 interactors
ELMiQ9Y6B2
IntActiQ9Y6B2, 10 interactors
MINTiQ9Y6B2
STRINGi9606.ENSP00000431162

Structurei

3D structure databases

ProteinModelPortaliQ9Y6B2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 120Interaction with NR0B2By similarityAdd BLAST67

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi178 – 182LXCXE motif1 Publication5

Phylogenomic databases

eggNOGiENOG410J09N Eukaryota
ENOG41118MI LUCA
GeneTreeiENSGT00500000045230
HOVERGENiHBG098825
InParanoidiQ9Y6B2
OMAiMAEPQGE
OrthoDBiEOG091G0YVS
PhylomeDBiQ9Y6B2
TreeFamiTF337633

Family and domain databases

InterProiView protein in InterPro
IPR033258 EID
IPR033255 EID-1
PANTHERiPTHR15556 PTHR15556, 1 hit
PTHR15556:SF5 PTHR15556:SF5, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.iShow all

Isoform 14 Publications (identifier: Q9Y6B2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSEMAELSEL YEESSDLQMD VMPGEGDLPQ MEVGSGSREL SLRPSRSGAQ
60 70 80 90 100
QLEEEGPMEE EEAQPMAAPE GKRSLANGPN AGEQPGQVAG ADFESEDEGE
110 120 130 140 150
EFDDWEDDYD YPEEEQLSGA GYRVSAALEE ADKMFLRTRE PALDGGFQMH
160 170 180
YEKTPFDQLA FIEELFSLMV VNRLTEELGC DEIIDRE
Length:187
Mass (Da):20,876
Last modified:November 1, 1999 - v1
Checksum:iA2815FA78ED0736D
GO
Isoform 21 Publication (identifier: Q9Y6B2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     65-87: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:164
Mass (Da):18,616
Checksum:iFB44C04D87381C53
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YN68H0YN68_HUMAN
EP300-interacting inhibitor of diff...
EID1
165Annotation score:

Sequence cautioni

The sequence AAK07524 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05245465 – 87Missing in isoform 2. 1 PublicationAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF349444 mRNA Translation: AAK29640.1
AF109873 mRNA Translation: AAG35179.1
AF092135 mRNA Translation: AAD40377.1
AL109701 mRNA Translation: CAB52022.1
AL357456 mRNA Translation: CAB93108.1
AK098383 mRNA Translation: BAC05296.1
AK315365 mRNA Translation: BAG37758.1
CH471082 Genomic DNA Translation: EAW77357.1
BC114944 mRNA Translation: AAI14945.1
BC114946 mRNA Translation: AAI14947.1
AF274947 mRNA Translation: AAK07524.1 Different initiation.
CCDSiCCDS53941.1 [Q9Y6B2-1]
RefSeqiNP_055150.1, NM_014335.2 [Q9Y6B2-1]
UniGeneiHs.255973

Genome annotation databases

EnsembliENST00000530028; ENSP00000431162; ENSG00000255302 [Q9Y6B2-1]
GeneIDi23741
KEGGihsa:23741
UCSCiuc001zxc.2 human [Q9Y6B2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEID1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6B2
Secondary accession number(s): B2RD11, Q8N7I4, Q9BZT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 1, 1999
Last modified: September 12, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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