EID1

Functionⁱ

Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.2 Publications

Miscellaneous

Inhibition of MYOD1 may be partly due to the ability of EID1 to bind and inhibit EP300 histone acetyltransferase activity.1 Publication

GO - Molecular functionⁱ

histone acetyltransferase binding
Source: UniProtKB
Inferred from direct assayⁱ
- 11073990
histone acetyltransferase regulator activity
Source: UniProtKB
Inferred from direct assayⁱ
- 11073990
transcription corepressor activity Source: UniProtKB

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell cycle Source: UniProtKB-KW
cell differentiation
Source: UniProtKB
Inferred from direct assayⁱ
- 11073989
negative regulation of transcription, DNA-templated
Source: UniProtKB
Inferred from direct assayⁱ
- 11073990
negative regulation of transcription by RNA polymerase II
Source: MGI
Inferred from direct assayⁱ
- 11073990
regulation of cell differentiation Source: InterPro
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Repressor
Biological process	Cell cycle, Differentiation, Transcription, Transcription regulation

Enzyme and pathway databases

SIGNORⁱ	Q9Y6B2

SIGNORⁱ

Q9Y6B2

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: EP300-interacting inhibitor of differentiation 1 Alternative name(s): 21 kDa pRb-associated protein CREBBP/EP300 inhibitory protein 1 E1A-like inhibitor of differentiation 1 Short name: EID-1
Gene namesⁱ	Name:EID1Imported Synonyms:C15orf3, CRI1Imported, RBP21 ORF Names:PNAS-22, PTD014
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 15

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000255302.3
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:1191 EID1
MIMⁱ	605894 gene
neXtProtⁱ	NX_Q9Y6B2

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	178	L → S: Abolishes RB1 binding. 1 Publication	1
Mutagenesisⁱ	180	C → G: Abolishes RB1 binding. 2 Publications	1
Mutagenesisⁱ	182	E → Q: Abolishes RB1 binding. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	23741
Open Targets More...OpenTargetsⁱ	ENSG00000255302
PharmGKBⁱ	PA26876

Polymorphism and mutation databases

BioMutaⁱ	EID1
DMDMⁱ	74721525

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000289156	1 – 187	EP300-interacting inhibitor of differentiation 1Add BLAST		187

Post-translational modificationⁱ

Ubiquitinated in U2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.1 Publication

Keywords - PTMⁱ

Ubl conjugation

Proteomic databases

PaxDbⁱ	Q9Y6B2
PeptideAtlas More...PeptideAtlasⁱ	Q9Y6B2
PRIDEⁱ	Q9Y6B2
ProteomicsDBⁱ	86644 86645 [Q9Y6B2-2]

PTM databases

iPTMnetⁱ	Q9Y6B2
PhosphoSitePlusⁱ	Q9Y6B2

Expressionⁱ

Tissue specificityⁱ

Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A-549 and various leukemia cell lines.2 Publications

Developmental stageⁱ

Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level).1 Publication

Inductionⁱ

Down-regulated in differentiating U-937 leukemia cells.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000255302 Expressed in 236 organ(s), highest expression level in tendon of biceps brachii
CleanExⁱ	HS_EID1
ExpressionAtlasⁱ	Q9Y6B2 baseline and differential
Genevisibleⁱ	Q9Y6B2 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA051122 HPA051123

HPAⁱ

HPA051122
HPA051123

Interactionⁱ

Subunit structureⁱ

Interacts via its LXCXE motif with the entire pocket region of RB1. Interacts with EP300, NR0B2 and TRIM27.By similarity4 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Ndn	P25233	5	EBI-1049975,EBI-1801080	From Mus musculus.

With

Entry

#Exp.

IntAct

Notes

Ndn

P25233

5

EBI-1049975,EBI-1801080

From Mus musculus.

GO - Molecular functionⁱ

histone acetyltransferase binding
Source: UniProtKB
Inferred from direct assayⁱ
- 11073990

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	117243, 48 interactors
ELMⁱ	Q9Y6B2
IntActⁱ	Q9Y6B2, 10 interactors
Molecular INTeraction database More...MINTⁱ	Q9Y6B2
STRINGⁱ	9606.ENSP00000431162

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9Y6B2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	54 – 120	Interaction with NR0B2By similarityAdd BLAST		67

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	178 – 182	LXCXE motif1 Publication		5

Phylogenomic databases

eggNOGⁱ	ENOG410J09N Eukaryota ENOG41118MI LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00500000045230
HOVERGENⁱ	HBG098825
InParanoidⁱ	Q9Y6B2
OMAⁱ	MAEPQGE
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0YVS
PhylomeDBⁱ	Q9Y6B2
TreeFamⁱ	TF337633

Family and domain databases

InterProⁱ	View protein in InterPro IPR033258 EID IPR033255 EID-1
PANTHERⁱ	PTHR15556 PTHR15556, 1 hit PTHR15556:SF5 PTHR15556:SF5, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.ⁱShow all

Isoform 14 Publications (identifier: Q9Y6B2-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEMAELSEL YEESSDLQMD VMPGEGDLPQ MEVGSGSREL SLRPSRSGAQ 
        60         70         80         90        100
QLEEEGPMEE EEAQPMAAPE GKRSLANGPN AGEQPGQVAG ADFESEDEGE 
       110        120        130        140        150
EFDDWEDDYD YPEEEQLSGA GYRVSAALEE ADKMFLRTRE PALDGGFQMH 
       160        170        180 
YEKTPFDQLA FIEELFSLMV VNRLTEELGC DEIIDRE

Length:187

Mass (Da):20,876

Last modified:November 1, 1999 - v1

Checksum:ⁱA2815FA78ED0736D

GO

Isoform 21 Publication (identifier: Q9Y6B2-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
65-87: Missing.

« Hide

        10         20         30         40         50
MSEMAELSEL YEESSDLQMD VMPGEGDLPQ MEVGSGSREL SLRPSRSGAQ 
        60         70         80         90        100
QLEEEGPMEE EEAQVAGADF ESEDEGEEFD DWEDDYDYPE EEQLSGAGYR 
       110        120        130        140        150
VSAALEEADK MFLRTREPAL DGGFQMHYEK TPFDQLAFIE ELFSLMVVNR 
       160 
LTEELGCDEI IDRE

Note: No experimental confirmation available.Curated

Show »

Length:164

Mass (Da):18,616

Checksum:ⁱFB44C04D87381C53

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation
	H0YN68	H0YN68_HUMAN		EP300-interacting inhibitor of diff... EP300-interacting inhibitor of differentiation 1	EID1	165	Annotation score:

Sequence cautionⁱ

The sequence AAK07524 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_052454	65 – 87	Missing in isoform 2. 1 PublicationAdd BLAST		23

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF349444 mRNA Translation: AAK29640.1 AF109873 mRNA Translation: AAG35179.1 AF092135 mRNA Translation: AAD40377.1 AL109701 mRNA Translation: CAB52022.1 AL357456 mRNA Translation: CAB93108.1 AK098383 mRNA Translation: BAC05296.1 AK315365 mRNA Translation: BAG37758.1 CH471082 Genomic DNA Translation: EAW77357.1 BC114944 mRNA Translation: AAI14945.1 BC114946 mRNA Translation: AAI14947.1 AF274947 mRNA Translation: AAK07524.1 Different initiation.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS53941.1 [Q9Y6B2-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_055150.1, NM_014335.2 [Q9Y6B2-1]
UniGeneⁱ	Hs.255973

Genome annotation databases

Ensemblⁱ	ENST00000530028; ENSP00000431162; ENSG00000255302 [Q9Y6B2-1]
GeneIDⁱ	23741
KEGGⁱ	hsa:23741
UCSC genome browser More...UCSCⁱ	uc001zxc.2 human [Q9Y6B2-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9Y6B2	EP300-interacting inhibitor of differentiation 1		PONAB		188	UniRef90_Q9Y6B2
EP300-interacting inhibitor of differentiation 1		CALJA		191
EP300-interacting inhibitor of differentiation 1		TARSY		191
Uncharacterized protein		OTOGA		191
EP300-interacting inhibitor of differentiation 1		MACMU		188
+18

Protein	Similar proteins		Species	Score	Length	Source
Q9Y6B2	EP300-interacting inhibitor of differentiation 1		PONAB		188	UniRef50_Q9Y6B2
EP300-interacting inhibitor of differentiation 1	)	MOUSE		169
EP300-interacting inhibitor of differentiation 1		TARSY		191
Uncharacterized protein		OTOGA		191
EP300-interacting inhibitor of differentiation 1		CALJA		191
+120

Entry informationⁱ

Entry nameⁱ	EID1_HUMAN
Accessionⁱ	Q9Y6B2Primary (citable) accession number: Q9Y6B2 Secondary accession number(s): B2RD11, Q8N7I4, Q9BZT9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
	Last sequence update:	November 1, 1999
	Last modified:	September 12, 2018
	This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9Y6B2 (EID1_HUMAN)

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EP300-interacting inhibitor of differentiation 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Motif

Phylogenomic databases

Family and domain databases

Computationally mapped potential isoform sequencesi

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Computationally mapped potential isoform sequencesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ