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Protein

Ancient ubiquitous protein 1

Gene

AUP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.1 Publication

GO - Molecular functioni

  • ubiquitin binding Source: GO_Central
  • ubiquitin-protein transferase activator activity Source: GO_Central

GO - Biological processi

  • retrograde protein transport, ER to cytosol Source: ParkinsonsUK-UCL
  • ubiquitin-dependent ERAD pathway Source: GO_Central

Keywordsi

Biological processUbl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ancient ubiquitous protein 1
Gene namesi
Name:AUP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115307.16
HGNCiHGNC:891 AUP1
MIMi602434 gene
neXtProtiNX_Q9Y679

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20LumenalSequence analysisAdd BLAST20
Transmembranei21 – 41Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini42 – 410CytoplasmicSequence analysisAdd BLAST369

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000115307
PharmGKBiPA25182

Polymorphism and mutation databases

BioMutaiAUP1
DMDMi12643958

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000207651 – 410Ancient ubiquitous protein 1Add BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei5PhosphoserineCombined sources1
Modified residuei288PhosphoserineCombined sources1
Modified residuei292PhosphoserineCombined sources1
Modified residuei363PhosphoserineCombined sources1
Modified residuei367PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Y679
PaxDbiQ9Y679
PeptideAtlasiQ9Y679
PRIDEiQ9Y679
ProteomicsDBi86616
86617 [Q9Y679-2]
86618 [Q9Y679-3]

PTM databases

iPTMnetiQ9Y679
PhosphoSitePlusiQ9Y679
SwissPalmiQ9Y679

Expressioni

Tissue specificityi

Detected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain.1 Publication

Gene expression databases

BgeeiENSG00000115307 Expressed in 227 organ(s), highest expression level in body of pancreas
CleanExiHS_AUP1
GenevisibleiQ9Y679 HS

Organism-specific databases

HPAiHPA007674

Interactioni

Subunit structurei

Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1. Interacts with the cytoplasmic tail of ITGA2B, ITGA1, ITGA2, ITGA5, ITGAV and ITGAM.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
OPRM1P353724EBI-1058701,EBI-2624570

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107031, 61 interactors
CORUMiQ9Y679
IntActiQ9Y679, 32 interactors
MINTiQ9Y679
STRINGi9606.ENSP00000366748

Structurei

Secondary structure

1410
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y679
SMRiQ9Y679
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y679

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini296 – 338CUEPROSITE-ProRule annotationAdd BLAST43

Sequence similaritiesi

Belongs to the AUP1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2898 Eukaryota
ENOG410Y6KF LUCA
GeneTreeiENSGT00390000016110
HOGENOMiHOG000034232
HOVERGENiHBG004321
InParanoidiQ9Y679
KOiK19716
OMAiLKFSSWP
OrthoDBiEOG091G0CVF
PhylomeDBiQ9Y679
TreeFamiTF313372

Family and domain databases

InterProiView protein in InterPro
IPR003892 CUE
PfamiView protein in Pfam
PF02845 CUE, 1 hit
SMARTiView protein in SMART
SM00546 CUE, 1 hit
PROSITEiView protein in PROSITE
PS51140 CUE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9Y679-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELPSGPGPE RLFDSHRLPG DCFLLLVLLL YAPVGFCLLV LRLFLGIHVF
60 70 80 90 100
LVSCALPDSV LRRFVVRTMC AVLGLVARQE DSGLRDHSVR VLISNHVTPF
110 120 130 140 150
DHNIVNLLTT CSTPLLNSPP SFVCWSRGFM EMNGRGELVE SLKRFCASTR
160 170 180 190 200
LPPTPLLLFP EEEATNGREG LLRFSSWPFS IQDVVQPLTL QVQRPLVSVT
210 220 230 240 250
VSDASWVSEL LWSLFVPFTV YQVRWLRPVH RQLGEANEEF ALRVQQLVAK
260 270 280 290 300
ELGQTGTRLT PADKAEHMKR QRHPRLRPQS AQSSFPPSPG PSPDVQLATL
310 320 330 340 350
AQRVKEVLPH VPLGVIQRDL AKTGCVDLTI TNLLEGAVAF MPEDITKGTQ
360 370 380 390 400
SLPTASASKF PSSGPVTPQP TALTFAKSSW ARQESLQERK QALYEYARRR
410
FTERRAQEAD
Length:410
Mass (Da):45,787
Last modified:September 12, 2018 - v2
Checksum:i1A06476225C5DFDC
GO
Isoform 2 (identifier: Q9Y679-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     360-373: FPSSGPVTPQPTAL → AFDACLMMMTPQAL
     374-410: Missing.

Show »
Length:373
Mass (Da):41,385
Checksum:iAAB256D01055538E
GO

Sequence cautioni

The sequence AAD43018 differs from that shown. Reason: Frameshift at position 114.Curated
The sequence AAH33646 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti288S → P in AAD43010 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_059683360 – 373FPSSG…QPTAL → AFDACLMMMTPQAL in isoform 2. Add BLAST14
Alternative sequenceiVSP_059684374 – 410Missing in isoform 2. Add BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100754 mRNA Translation: AAD43018.1 Frameshift.
AF100753 mRNA Translation: AAD43017.1
AF100746 mRNA Translation: AAD43010.1
AF165515 mRNA Translation: AAF86645.1
AK023983 mRNA Translation: BAB14753.1
CH471053 Genomic DNA Translation: EAW99623.1
BC001658 mRNA Translation: AAH01658.1
BC033646 mRNA Translation: AAH33646.2 Different initiation.
CCDSiCCDS42702.1 [Q9Y679-2]
RefSeqiNP_853553.1, NM_181575.4 [Q9Y679-2]
UniGeneiHs.411480

Genome annotation databases

EnsembliENST00000377526; ENSP00000366748; ENSG00000115307 [Q9Y679-2]
ENST00000425118; ENSP00000403430; ENSG00000115307 [Q9Y679-3]
GeneIDi550
KEGGihsa:550
UCSCiuc002smf.4 human [Q9Y679-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100754 mRNA Translation: AAD43018.1 Frameshift.
AF100753 mRNA Translation: AAD43017.1
AF100746 mRNA Translation: AAD43010.1
AF165515 mRNA Translation: AAF86645.1
AK023983 mRNA Translation: BAB14753.1
CH471053 Genomic DNA Translation: EAW99623.1
BC001658 mRNA Translation: AAH01658.1
BC033646 mRNA Translation: AAH33646.2 Different initiation.
CCDSiCCDS42702.1 [Q9Y679-2]
RefSeqiNP_853553.1, NM_181575.4 [Q9Y679-2]
UniGeneiHs.411480

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EKFNMR-A292-345[»]
ProteinModelPortaliQ9Y679
SMRiQ9Y679
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107031, 61 interactors
CORUMiQ9Y679
IntActiQ9Y679, 32 interactors
MINTiQ9Y679
STRINGi9606.ENSP00000366748

PTM databases

iPTMnetiQ9Y679
PhosphoSitePlusiQ9Y679
SwissPalmiQ9Y679

Polymorphism and mutation databases

BioMutaiAUP1
DMDMi12643958

Proteomic databases

EPDiQ9Y679
PaxDbiQ9Y679
PeptideAtlasiQ9Y679
PRIDEiQ9Y679
ProteomicsDBi86616
86617 [Q9Y679-2]
86618 [Q9Y679-3]

Protocols and materials databases

DNASUi550
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000377526; ENSP00000366748; ENSG00000115307 [Q9Y679-2]
ENST00000425118; ENSP00000403430; ENSG00000115307 [Q9Y679-3]
GeneIDi550
KEGGihsa:550
UCSCiuc002smf.4 human [Q9Y679-2]

Organism-specific databases

CTDi550
EuPathDBiHostDB:ENSG00000115307.16
GeneCardsiAUP1
HGNCiHGNC:891 AUP1
HPAiHPA007674
MIMi602434 gene
neXtProtiNX_Q9Y679
OpenTargetsiENSG00000115307
PharmGKBiPA25182
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2898 Eukaryota
ENOG410Y6KF LUCA
GeneTreeiENSGT00390000016110
HOGENOMiHOG000034232
HOVERGENiHBG004321
InParanoidiQ9Y679
KOiK19716
OMAiLKFSSWP
OrthoDBiEOG091G0CVF
PhylomeDBiQ9Y679
TreeFamiTF313372

Miscellaneous databases

ChiTaRSiAUP1 human
EvolutionaryTraceiQ9Y679
GeneWikiiAUP1
GenomeRNAii550
PROiPR:Q9Y679
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115307 Expressed in 227 organ(s), highest expression level in body of pancreas
CleanExiHS_AUP1
GenevisibleiQ9Y679 HS

Family and domain databases

InterProiView protein in InterPro
IPR003892 CUE
PfamiView protein in Pfam
PF02845 CUE, 1 hit
SMARTiView protein in SMART
SM00546 CUE, 1 hit
PROSITEiView protein in PROSITE
PS51140 CUE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAUP1_HUMAN
AccessioniPrimary (citable) accession number: Q9Y679
Secondary accession number(s): C0H5W8
, Q9H866, Q9UNQ6, Q9Y685
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: September 12, 2018
Last modified: November 7, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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