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Protein

Heparan sulfate glucosamine 3-O-sulfotransferase 3A1

Gene

HS3ST3A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in IdoUA2S-GlcNS and also in IdoUA2S-GlcNH2. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes simplex virus-1 (HSV-1) and permits its entry. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei166Substrate1
Binding sitei243PAPS1
Binding sitei251PAPS1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi162 – 166PAPS5
Nucleotide bindingi368 – 372PAPS5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BioCyciMetaCyc:HS07937-MONOMER
ReactomeiR-HSA-2022928 HS-GAG biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan sulfate glucosamine 3-O-sulfotransferase 3A1 (EC:2.8.2.30)
Alternative name(s):
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3A1
Short name:
3-OST-3A
Short name:
Heparan sulfate 3-O-sulfotransferase 3A1
Short name:
h3-OST-3A
Gene namesi
Name:HS3ST3A1
Synonyms:3OST3A1, HS3ST3A
ORF Names:UNQ2551/PRO6180
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000153976.2
HGNCiHGNC:5196 HS3ST3A1
MIMi604057 gene
neXtProtiNX_Q9Y663

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24CytoplasmicSequence analysisAdd BLAST24
Transmembranei25 – 43Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST19
Topological domaini44 – 406LumenalSequence analysisAdd BLAST363

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi161K → A: 99.6% loss of enzymatic activity. 1 Publication1
Mutagenesisi162K → A: 99.6% loss of enzymatic activity; no HSV1 entry activity. 2 Publications1
Mutagenesisi166R → E: 99.8% loss of enzymatic activity. 1 Publication1
Mutagenesisi170E → Q: 17% loss of enzymatic activity. 1 Publication1
Mutagenesisi173R → S: 44.1% loss of enzymatic activity. 1 Publication1
Mutagenesisi182G → A: No effect on enzymatic activity. 1 Publication1
Mutagenesisi184E → Q: 99.9% loss of enzymatic activity. 1 Publication1
Mutagenesisi186H → F: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi189D → N: 99.1% loss of enzymatic activity. 1 Publication1
Mutagenesisi190R → E: 32% loss of enzymatic activity. 1 Publication1
Mutagenesisi194K → A: 99.5% loss of enzymatic activity. 1 Publication1
Mutagenesisi215K → A: 99.9% loss of enzymatic activity. 1 Publication1
Mutagenesisi218S → A: 23.3% loss of enzymatic activity. 1 Publication1
Mutagenesisi224E → Q: 47.6% loss of enzymatic activity. 1 Publication1
Mutagenesisi255Q → A: 99.6% loss of enzymatic activity. 1 Publication1
Mutagenesisi259K → A: 48.3% loss of enzymatic activity. 1 Publication1
Mutagenesisi288I → A: 65% loss of enzymatic activity. 1 Publication1
Mutagenesisi293K → A: 33.6% loss of enzymatic activity. 1 Publication1
Mutagenesisi362H → A: No effect on enzymatic activity. 1 Publication1
Mutagenesisi365G → A: 43% loss of enzymatic activity. 1 Publication1
Mutagenesisi366K → A: 99.8% loss of enzymatic activity. 1 Publication1
Mutagenesisi368K → A: 99.9% loss of enzymatic activity. 1 Publication1
Mutagenesisi370R → E: 99.2% loss of enzymatic activity. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000153976
PharmGKBiPA29469

Chemistry databases

DrugBankiDB01812 Adenosine-3'-5'-Diphosphate
DB04272 Citric Acid
DB02264 O2-Sulfo-Glucuronic Acid

Polymorphism and mutation databases

BioMutaiHS3ST3A1
DMDMi61214551

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000852171 – 406Heparan sulfate glucosamine 3-O-sulfotransferase 3A1Add BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi273N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi344N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi351 ↔ 3631 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9Y663
PeptideAtlasiQ9Y663
PRIDEiQ9Y663
ProteomicsDBi86606

PTM databases

iPTMnetiQ9Y663
PhosphoSitePlusiQ9Y663

Expressioni

Tissue specificityi

Ubiquitous. Most abundant in heart and placenta, followed by liver and kidney.1 Publication

Gene expression databases

BgeeiENSG00000153976 Expressed in 133 organ(s), highest expression level in tibia
CleanExiHS_HS3ST3A1
ExpressionAtlasiQ9Y663 baseline and differential
GenevisibleiQ9Y663 HS

Organism-specific databases

HPAiHPA062518
HPA071530

Interactioni

Protein-protein interaction databases

BioGridi115280, 1 interactor
STRINGi9606.ENSP00000284110

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9Y663
SMRiQ9Y663
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y663

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 190Substrate binding7
Regioni215 – 218Substrate binding4
Regioni255 – 259Substrate binding5
Regioni283 – 284Substrate binding2
Regioni367 – 370Substrate binding4

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3704 Eukaryota
ENOG410XS59 LUCA
GeneTreeiENSGT00760000119023
HOGENOMiHOG000036663
HOVERGENiHBG053377
InParanoidiQ9Y663
KOiK07809
OMAiHCLGKNK
OrthoDBiEOG091G0CS5
PhylomeDBiQ9Y663
TreeFamiTF350755

Family and domain databases

InterProiView protein in InterPro
IPR037359 NST/OST
IPR027417 P-loop_NTPase
IPR000863 Sulfotransferase_dom
PANTHERiPTHR10605 PTHR10605, 1 hit
PfamiView protein in Pfam
PF00685 Sulfotransfer_1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9Y663-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPPGPASAL STSAEPLSRS IFRKFLLMLC SLLTSLYVFY CLAERCQTLS
60 70 80 90 100
GPVVGLSGGG EEAGAPGGGV LAGGPRELAV WPAAAQRKRL LQLPQWRRRR
110 120 130 140 150
PPAPRDDGEE AAWEEESPGL SGGPGGSGAG STVAEAPPGT LALLLDEGSK
160 170 180 190 200
QLPQAIIIGV KKGGTRALLE FLRVHPDVRA VGAEPHFFDR SYDKGLAWYR
210 220 230 240 250
DLMPRTLDGQ ITMEKTPSYF VTREAPARIS AMSKDTKLIV VVRDPVTRAI
260 270 280 290 300
SDYTQTLSKR PDIPTFESLT FKNRTAGLID TSWSAIQIGI YAKHLEHWLR
310 320 330 340 350
HFPIRQMLFV SGERLISDPA GELGRVQDFL GLKRIITDKH FYFNKTKGFP
360 370 380 390 400
CLKKAEGSSR PHCLGKTKGR THPEIDREVV RRLREFYRPF NLKFYQMTGH

DFGWDG
Length:406
Mass (Da):44,900
Last modified:November 1, 1999 - v1
Checksum:i57B6A0ABC897577A
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KSX5J3KSX5_HUMAN
Sulfotransferase
HS3ST3A1
204Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105376 mRNA Translation: AAD30208.1
AY358838 mRNA Translation: AAQ89197.1
AK292047 mRNA Translation: BAF84736.1
CH471108 Genomic DNA Translation: EAW89959.1
BC044647 mRNA Translation: AAH44647.1
CCDSiCCDS11165.1
RefSeqiNP_006033.1, NM_006042.2
UniGeneiHs.462270
Hs.562745

Genome annotation databases

EnsembliENST00000284110; ENSP00000284110; ENSG00000153976
GeneIDi9955
KEGGihsa:9955
UCSCiuc002gob.1 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF105376 mRNA Translation: AAD30208.1
AY358838 mRNA Translation: AAQ89197.1
AK292047 mRNA Translation: BAF84736.1
CH471108 Genomic DNA Translation: EAW89959.1
BC044647 mRNA Translation: AAH44647.1
CCDSiCCDS11165.1
RefSeqiNP_006033.1, NM_006042.2
UniGeneiHs.462270
Hs.562745

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T8TX-ray1.85A/B139-406[»]
1T8UX-ray1.95A/B139-406[»]
ProteinModelPortaliQ9Y663
SMRiQ9Y663
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115280, 1 interactor
STRINGi9606.ENSP00000284110

Chemistry databases

DrugBankiDB01812 Adenosine-3'-5'-Diphosphate
DB04272 Citric Acid
DB02264 O2-Sulfo-Glucuronic Acid

PTM databases

iPTMnetiQ9Y663
PhosphoSitePlusiQ9Y663

Polymorphism and mutation databases

BioMutaiHS3ST3A1
DMDMi61214551

Proteomic databases

PaxDbiQ9Y663
PeptideAtlasiQ9Y663
PRIDEiQ9Y663
ProteomicsDBi86606

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000284110; ENSP00000284110; ENSG00000153976
GeneIDi9955
KEGGihsa:9955
UCSCiuc002gob.1 human

Organism-specific databases

CTDi9955
EuPathDBiHostDB:ENSG00000153976.2
GeneCardsiHS3ST3A1
HGNCiHGNC:5196 HS3ST3A1
HPAiHPA062518
HPA071530
MIMi604057 gene
neXtProtiNX_Q9Y663
OpenTargetsiENSG00000153976
PharmGKBiPA29469
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3704 Eukaryota
ENOG410XS59 LUCA
GeneTreeiENSGT00760000119023
HOGENOMiHOG000036663
HOVERGENiHBG053377
InParanoidiQ9Y663
KOiK07809
OMAiHCLGKNK
OrthoDBiEOG091G0CS5
PhylomeDBiQ9Y663
TreeFamiTF350755

Enzyme and pathway databases

BioCyciMetaCyc:HS07937-MONOMER
ReactomeiR-HSA-2022928 HS-GAG biosynthesis

Miscellaneous databases

ChiTaRSiHS3ST3A1 human
EvolutionaryTraceiQ9Y663
GeneWikiiHS3ST3A1
GenomeRNAii9955
PROiPR:Q9Y663
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000153976 Expressed in 133 organ(s), highest expression level in tibia
CleanExiHS_HS3ST3A1
ExpressionAtlasiQ9Y663 baseline and differential
GenevisibleiQ9Y663 HS

Family and domain databases

InterProiView protein in InterPro
IPR037359 NST/OST
IPR027417 P-loop_NTPase
IPR000863 Sulfotransferase_dom
PANTHERiPTHR10605 PTHR10605, 1 hit
PfamiView protein in Pfam
PF00685 Sulfotransfer_1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHS3SA_HUMAN
AccessioniPrimary (citable) accession number: Q9Y663
Secondary accession number(s): A8K7N2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1999
Last modified: September 12, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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