UniProtKB - Q9Y5Y2 (NUBP2_HUMAN)
Protein
Cytosolic Fe-S cluster assembly factor NUBP2
Gene
NUBP2
Organism
Homo sapiens (Human)
Status
Functioni
Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.UniRule annotationBy similarity
Cofactori
[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 196 | Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation | 1 | |
Metal bindingi | 199 | Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 22 – 29 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-KW
- iron-sulfur cluster binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- nucleotide binding Source: ProtInc
GO - Biological processi
- cell projection organization Source: UniProtKB-KW
- iron-sulfur cluster assembly Source: GO_Central
Keywordsi
Biological process | Cilium biogenesis/degradation |
Ligand | 4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9Y5Y2 |
Reactomei | R-HSA-2564830, Cytosolic iron-sulfur cluster assembly |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic Fe-S cluster assembly factor NUBP2UniRule annotationAlternative name(s): Nucleotide-binding protein 2UniRule annotation Short name: NBP 2UniRule annotation |
Gene namesi | Name:NUBP2UniRule annotation |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8042, NUBP2 |
MIMi | 610779, gene |
neXtProti | NX_Q9Y5Y2 |
VEuPathDBi | HostDB:ENSG00000095906.16 |
Subcellular locationi
Cytoskeleton
- centrosome UniRule annotation
- cilium axoneme By similarity
- centriole By similarity
- microtubule organizing center By similarity
Nucleus
- Nucleus UniRule annotation
Other locations
- Cytoplasm 1 Publication
Note: Enriched at the centrosomes during mitosis. Enriched in centrioles of microtubule asters during prophase, prometaphase and telophase stages of mitosis (By similarity). Localized at centrioles and in the nucleus at interphase (By similarity). Colocalizes with nubp-1 at prometaphase (By similarity).UniRule annotationBy similarity
Cytoskeleton
- centriole Source: UniProtKB-SubCell
- spindle pole centrosome Source: Ensembl
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- cilium Source: UniProtKB-KW
Keywords - Cellular componenti
Cell projection, Cilium, Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 10101 |
OpenTargetsi | ENSG00000095906 |
PharmGKBi | PA31824 |
Miscellaneous databases
Pharosi | Q9Y5Y2, Tbio |
Genetic variation databases
BioMutai | NUBP2 |
DMDMi | 13632176 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000184945 | 1 – 271 | Cytosolic Fe-S cluster assembly factor NUBP2Add BLAST | 271 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineUniRule annotationCombined sources1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9Y5Y2 |
jPOSTi | Q9Y5Y2 |
MassIVEi | Q9Y5Y2 |
MaxQBi | Q9Y5Y2 |
PaxDbi | Q9Y5Y2 |
PeptideAtlasi | Q9Y5Y2 |
PRIDEi | Q9Y5Y2 |
ProteomicsDBi | 86538 |
PTM databases
iPTMneti | Q9Y5Y2 |
PhosphoSitePlusi | Q9Y5Y2 |
Expressioni
Tissue specificityi
Widely expressed with highest expression in skeletal muscle.
Developmental stagei
Expressed in fetal brain, lung, liver and kidney.
Gene expression databases
Bgeei | ENSG00000095906, Expressed in stomach and 121 other tissues |
ExpressionAtlasi | Q9Y5Y2, baseline and differential |
Organism-specific databases
HPAi | ENSG00000095906, Low tissue specificity |
Interactioni
Subunit structurei
Heterotetramer of 2 NUBP1 and 2 NUBP2 chains (By similarity).
Interacts with KIFC1 (By similarity).
Interacts with NUBP1 (PubMed:18573874).
UniRule annotationBy similarity1 PublicationBinary interactionsi
Hide detailsQ9Y5Y2
Protein-protein interaction databases
BioGRIDi | 115408, 86 interactors |
IntActi | Q9Y5Y2, 21 interactors |
MINTi | Q9Y5Y2 |
STRINGi | 9606.ENSP00000262302 |
Miscellaneous databases
RNActi | Q9Y5Y2, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3022, Eukaryota |
GeneTreei | ENSGT00950000183193 |
HOGENOMi | CLU_024839_0_1_1 |
InParanoidi | Q9Y5Y2 |
OMAi | SDEHMAV |
OrthoDBi | 1166096at2759 |
PhylomeDBi | Q9Y5Y2 |
TreeFami | TF354321 |
Family and domain databases
HAMAPi | MF_02040, Mrp_NBP35, 1 hit MF_03039, NUBP2, 1 hit |
InterProi | View protein in InterPro IPR019591, Mrp/NBP35_ATP-bd IPR000808, Mrp_CS IPR028600, NUBP2/Cfd1_eukaryotes IPR027417, P-loop_NTPase IPR033756, YlxH/NBP35 |
PANTHERi | PTHR23264, PTHR23264, 1 hit |
Pfami | View protein in Pfam PF10609, ParA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS01215, MRP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All
Q9Y5Y2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEAAAEPGNL AGVRHIILVL SGKGGVGKST ISTELALALR HAGKKVGILD
60 70 80 90 100
VDLCGPSIPR MLGAQGRAVH QCDRGWAPVF LDREQSISLM SVGFLLEKPD
110 120 130 140 150
EAVVWRGPKK NALIKQFVSD VAWGELDYLV VDTPPGTSDE HMATIEALRP
160 170 180 190 200
YQPLGALVVT TPQAVSVGDV RRELTFCRKT GLRVMGIVEN MSGFTCPHCT
210 220 230 240 250
ECTSVFSRGG GEELAQLAGV PFLGSVPLDP ALMRTLEEGH DFIQEFPGSP
260 270
AFAALTSIAQ KILDATPACL P
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BNF0 | H3BNF0_HUMAN | Cytosolic Fe-S cluster assembly fac... | NUBP2 | 251 | Annotation score: | ||
B7Z6P0 | B7Z6P0_HUMAN | Cytosolic Fe-S cluster assembly fac... | NUBP2 hCG_42691 | 130 | Annotation score: | ||
H3BQR2 | H3BQR2_HUMAN | Cytosolic Fe-S cluster assembly fac... | NUBP2 | 211 | Annotation score: | ||
H3BNS4 | H3BNS4_HUMAN | Cytosolic Fe-S cluster assembly fac... | NUBP2 | 271 | Annotation score: | ||
H3BRE1 | H3BRE1_HUMAN | Cytosolic Fe-S cluster assembly fac... | NUBP2 | 144 | Annotation score: | ||
H3BMW1 | H3BMW1_HUMAN | Cytosolic Fe-S cluster assembly fac... | NUBP2 | 139 | Annotation score: | ||
H3BRK5 | H3BRK5_HUMAN | Cytosolic Fe-S cluster assembly fac... | NUBP2 | 46 | Annotation score: | ||
H3BQE5 | H3BQE5_HUMAN | Cytosolic Fe-S cluster assembly fac... | NUBP2 | 58 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 242 | F → S in BAA91471 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_061353 | 200 | T → A. Corresponds to variant dbSNP:rs57822546Ensembl. | 1 | |
Natural variantiVAR_050099 | 250 | P → S. Corresponds to variant dbSNP:rs35030308Ensembl. | 1 | |
Natural variantiVAR_050100 | 266 | T → M. Corresponds to variant dbSNP:rs34028164Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF118394 mRNA Translation: AAD45242.1 AK001023 mRNA Translation: BAA91471.1 CH471112 Genomic DNA Translation: EAW85613.1 CH471112 Genomic DNA Translation: EAW85617.1 BC002768 mRNA Translation: AAH02768.1 BC008005 mRNA Translation: AAH08005.1 |
CCDSi | CCDS10445.1 |
RefSeqi | NP_001271430.1, NM_001284501.1 NP_036357.1, NM_012225.3 |
Genome annotation databases
Ensembli | ENST00000262302; ENSP00000262302; ENSG00000095906 |
GeneIDi | 10101 |
KEGGi | hsa:10101 |
UCSCi | uc002cmw.6, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF118394 mRNA Translation: AAD45242.1 AK001023 mRNA Translation: BAA91471.1 CH471112 Genomic DNA Translation: EAW85613.1 CH471112 Genomic DNA Translation: EAW85617.1 BC002768 mRNA Translation: AAH02768.1 BC008005 mRNA Translation: AAH08005.1 |
CCDSi | CCDS10445.1 |
RefSeqi | NP_001271430.1, NM_001284501.1 NP_036357.1, NM_012225.3 |
3D structure databases
SMRi | Q9Y5Y2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 115408, 86 interactors |
IntActi | Q9Y5Y2, 21 interactors |
MINTi | Q9Y5Y2 |
STRINGi | 9606.ENSP00000262302 |
PTM databases
iPTMneti | Q9Y5Y2 |
PhosphoSitePlusi | Q9Y5Y2 |
Genetic variation databases
BioMutai | NUBP2 |
DMDMi | 13632176 |
Proteomic databases
EPDi | Q9Y5Y2 |
jPOSTi | Q9Y5Y2 |
MassIVEi | Q9Y5Y2 |
MaxQBi | Q9Y5Y2 |
PaxDbi | Q9Y5Y2 |
PeptideAtlasi | Q9Y5Y2 |
PRIDEi | Q9Y5Y2 |
ProteomicsDBi | 86538 |
Protocols and materials databases
Antibodypediai | 23260, 81 antibodies |
Genome annotation databases
Ensembli | ENST00000262302; ENSP00000262302; ENSG00000095906 |
GeneIDi | 10101 |
KEGGi | hsa:10101 |
UCSCi | uc002cmw.6, human |
Organism-specific databases
CTDi | 10101 |
DisGeNETi | 10101 |
GeneCardsi | NUBP2 |
HGNCi | HGNC:8042, NUBP2 |
HPAi | ENSG00000095906, Low tissue specificity |
MIMi | 610779, gene |
neXtProti | NX_Q9Y5Y2 |
OpenTargetsi | ENSG00000095906 |
PharmGKBi | PA31824 |
VEuPathDBi | HostDB:ENSG00000095906.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3022, Eukaryota |
GeneTreei | ENSGT00950000183193 |
HOGENOMi | CLU_024839_0_1_1 |
InParanoidi | Q9Y5Y2 |
OMAi | SDEHMAV |
OrthoDBi | 1166096at2759 |
PhylomeDBi | Q9Y5Y2 |
TreeFami | TF354321 |
Enzyme and pathway databases
PathwayCommonsi | Q9Y5Y2 |
Reactomei | R-HSA-2564830, Cytosolic iron-sulfur cluster assembly |
Miscellaneous databases
BioGRID-ORCSi | 10101, 541 hits in 891 CRISPR screens |
ChiTaRSi | NUBP2, human |
GeneWikii | NUBP2 |
GenomeRNAii | 10101 |
Pharosi | Q9Y5Y2, Tbio |
PROi | PR:Q9Y5Y2 |
RNActi | Q9Y5Y2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000095906, Expressed in stomach and 121 other tissues |
ExpressionAtlasi | Q9Y5Y2, baseline and differential |
Family and domain databases
HAMAPi | MF_02040, Mrp_NBP35, 1 hit MF_03039, NUBP2, 1 hit |
InterProi | View protein in InterPro IPR019591, Mrp/NBP35_ATP-bd IPR000808, Mrp_CS IPR028600, NUBP2/Cfd1_eukaryotes IPR027417, P-loop_NTPase IPR033756, YlxH/NBP35 |
PANTHERi | PTHR23264, PTHR23264, 1 hit |
Pfami | View protein in Pfam PF10609, ParA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS01215, MRP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUBP2_HUMAN | |
Accessioni | Q9Y5Y2Primary (citable) accession number: Q9Y5Y2 Secondary accession number(s): D3DU80, Q9NWB2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 10, 2021 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families